ACS Applied Bio Materials
·
2019
·
IF 0
Peihong Li,Chunling Zhang,Rui Li,Lijie Qu,Xueyan Dai,Yanlong Sui,Jiazi Hou
Pluronic F127 is a thermosensitive polymer that has been extensively studied and utilized in biomedicine. To improve the gelation properties of F127, α-cyclodextrin was introduced by physical interactions, such as forming inclusion complexes, hydrogen bond self-assembly, and hydrophobic interactions, to prepare F127−α-CD hydrogels. This study explored the temperature-dependent sol–gel transition behavior, gelation mechanism, interior morphology, and controlled release of the anticancer drug 5-fluorouracil. Results showed that hydrogel could be obtained at 1.0%–8.0% weight content of F127. The cross-linking points in this system were PEO−α-CD microcrystalline region and micelle with poly(propylene oxide) as the core. The network inside F127−α-CD hydrogels made it stable and conducive for controlled release. Therefore, this hydrogel is a promising drug release system.
研究领域:
药剂学
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Chong Sun,Muhan Zhang,Qiqi Fei,Daoying Wang,Zhilan Sun,Zhiming Geng,Weimin Xu,Fang Liu
In this paper, a novel immunosensor was built for quantitative detection of heat shock protein 90 (HSP90) based on graphite-like carbon nitride nanosheets (g-C 3 N 4 NSs) hybridized with gold-F127 nanoparticles (g-C 3 N 4 -F127-Au NSs) modified electrode. The transmission electron microscope (TEM) was used to observe the structure and morphology of g-C 3 N 4 -F127-Au NSs. The successful preparation of g-C 3 N 4 -F127-Au NSs was characterized by UV–vis spectra, fourier transform infrared spectra (FTIR) and Raman spectra. Meanwhile, the electrochemical properties were measured by linear sweep voltammetric (LSV) and differential pulse voltammetry (DPV). Under the optimized conditions, the peak currents were linear with the concentration of HSP90 ranging from 3.5 μg/mL to 2.43 mg/mL. The linear regression equation was I (μA) = 10.31–10.05logc (R 2 = 0.9986, n = 9) with the detection limit of 2.67 μg/mL. Finally, the immunosensor exhibited good selectivity performance and provided a new approach for meat quality evaluation.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
·
2025
·
IF 2.9
Li Caixia,Zhou Qin,Chao Zhenzhen,Li Liang
In this study, the preparation of pH- and reduction-responsive doxorubicin (DOX)-loaded hyaluronate acid-F127 supramolecular hydrogels (DOX@Gels) with targeting ability due to the incorporation of hyaluronic acid is described. By combining Pluronic F127-modified hyaluronic acid (HA-F127), disulfide-modified β-cyclodextrin, α-cyclodextrin, and DOX, hydrogels were constructed through host–guest complexation and hydrogen bonding interactions, and a tailored platform for targeted drug delivery was provided. The chemical composition and rheological properties were comprehensively analyzed. Considering the controlled complexation between DOX and the hydrogel skeleton and the ability of HA to target cancer cells, controlled drug release from the hydrogel G[15,5,5] was investigated under various conditions in vitro. The cumulative amount of DOX released from hydrogel G[15,5,5] showed superior pH- and reduction-controlled release behavior. These results were likely caused by the HA-F127 ester bonds cleavage in acidic environments and the S‒S bond cleavage within the hydrogel networks in the presence of glutathione. Furthermore, the DOX release kinetics were analyzed using Higuchi and Korsmeyer-Peppas models. The results demonstrated that the experimental data was effectively fit with these two models, and the data fitted by using Korsmeyer-Peppas also indicated nonFickian diffusion kinetics of the DOX being released from hydrogels. Owing to the ability of HA to target SKOV-3 cells, the cytotoxicity experiments revealed that DOX@G[15,5,5] exhibited more significant anticancer effects than free DOX. Consequently, DOX@Gels containing HA have emerged as promising candidates for the targeted delivery and release of anticancer drugs.
研究领域:
药剂学
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生物材料
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2015
·
IF 3.545
Juan Li,Hangqing Xie,Yuhong Liu,Hang Ren,Wenbo Zhao,Xiaohua Huang
Nanomaterial-based signal-amplification strategies hold a great promise in realizing sensitive biological detection. A simple label-free electrochemical immunosensor for sensitive detection of carcinoembryonic antigen (CEA) was developed by immobilizing anti-CEA antibodies onto the Au-F127 strawberry-like nanospheres modified glassy carbon electrode (Au-F127/GCE). The Au-F127 strawberry-like nanospheres offered a large surface and multifunctional substrate for the effective immobilization of anti-CEA and the existence of Au could accelerate electron transfer and make the electrochemical signal amplified. The Au-F127 nanocomposites and anti-CEA were characterized by transmission electron microscopy (TEM), polycrystalline electron diffraction ring pattern, ultra-violet visible (UV–vis) spectra and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were employed to verify the stepwise assembly of the immunosensor and evaluated the analytical performance of the fabricated immunosensor, respectively. The immunosensor showed a wide liner response range between 0.01 and 80 ng mL −1 with a low detection limit of 0.24 pg mL −1 at a signal-to-noise (S/N) ratio of 3. Additionally, the proposed method was successfully applied to determine CEA in human serum samples with satisfactory results.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 3
·1461-1471
·
IF 3.361
Wenzhao Han,Fanwei Meng,Hao Gan,Feng Guo,Junfeng Ke,Liping Wang
The treatment of breast cancer mainly relies on chemotherapy drugs, which present significant side effects. The most typical example is the cardiotoxicity and bone marrow suppression associated with doxorubicin (DOX). Therefore, this drug is not the first choice in clinical treatment. We designed ATN-FFPFF-ATN, a new targeted antitumor drug carrier, polymerized from phenylalanine dipeptide (FF), ATN-161 peptide, and Pluronic® F-127. The peptide and Pluronic® F-127 are linked with acetal and are, therefore, acid-sensitive. As cancer can reduce pH through complex mechanisms and subsequently maintain acid ambience, our vehicle can smartly unravel at a peculiar position, through which the drug can specifically accumulate inside the tumor. ATN-161 is a protein ligand of integrin α5β1, which is highly expressed on the surface of some breast cancer cells. This targeting peptide sequence can play a role in the selective delivery of DOX to tumor cells. The DOX-carrying vector was able to significantly inhibit cell proliferation and promote cell apoptosis in MDA-MB-231 cells. Based on these results, ATN-FFPFF-ATN with pH response is a promising vehicle for DOX delivery.
研究领域:
药学
·
癌症研究
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR AND CELLULAR BIOCHEMISTRY
·
2022
·Vol. 478
·期 2
·241-247
·
IF 3.842
Zhou Quan,Cai Xu,Huang Ying,Zhou Youliang
Curcumin (CUR) is an extract of Curcuma longa Linn., which has various pharmacological activities. The instability, low water solubility and bioavailability of CUR greatly limit its clinical application. This work prepared Pluronic F127-liposome-encapsulated curcumin (CUR-LIP-F127) and explored its functional role in wound healing. Liposome-encapsulated curcumin (CUR-LIP) and CUR-LIP-F127 were prepared. Human keratinocyte cell line (HaCaT) was treated with CUR, Pluronic F127-liposome (LIP-F127) and CUR-LIP-F127, or combined with ML385 (Nrf2 inhibitor). The expression of mRNAs and proteins was detected by quantitative real-time PCR and western blotting. MTT and wound healing assays were performed to detect cell viability and migration. CUR, LIP-F127 and CUR-LIP-F127 all had no influence on cell viability of HaCaT cells. CUR-LIP-F127 treatment significantly accelerated cell migration and enhanced the expression of nuclear factor erythroid-related factor 2 (Nrf2) and kelch-like erythroid cell-derived protein 1 (Keap1) in HaCaT cells with respect to CUR or LIP-F127 treatment. ML385 treatment impaired CUR-LIP-F127-mediated promotion of migration and up-regulation of Nrf2 and Keap1 in HaCaT cells. This work demonstrated that CUR-LIP-F127 activated Nrf2/Keap1 signaling pathway to promote migration of HaCaT cells, suggesting that CUR-LIP-F127 may contribute to wound healing.
研究领域:
药理学
·
细胞生物学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2017
·Vol. 35
·期 7
·1109-1116
·
IF 1.852
Naixu Li,Jun Yin,Lingfei Wei,Quanhao Shen,Wei Tian,Jing Li,Yong Chen,Jing Jin,Hongcheng Teng,Jiancheng Zhou
We fabricate a novel cellulose acetate (CA) ultrafiltration membrane modified by block copolymer F127-b- PDMAEMA, which is synthesized using F127 and DMAEMA via the ARGET ATRP method. Compared to conventional ultrafiltration membranes, the incorporation of both F127 and PDMAEMA can not only readily increase the hydrophilicity of the membrane, but also exhibit stimuli-responsiveness to temperature and pH. Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR), and gel permeation chromatography (GPC) are employed to analyze the structure of the F127-b-PDMAEMA. The membrane properties are evaluated via scanning electron microscope (SEM) imaging, porosity test, automatic target recognition Fourier transform infrared spectroscopy (ATR-FTIR), water contact angle test and permeation test. The results indicate that the F127-b-PDMAEMA is an excellent pore agent, which contributes to an enhancement of the membrane in sensitivity to temperature and pH. The modified membrane also exhibits lower water contact angle (64.5°), which is attributed to the good anti-fouling performance and high water permeation.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Science of Advanced Materials
·
2019
·Vol. 11
·期 2
·166-177
·
IF 1.158
Cao Zhenhao,Li Wenting,Pang Juanjuan,Xu Jingjing,Jiao Yapei,Li Xue
Polyethyleneimine (PEI) modified PluronicF127 block copolymers (PEI-F127-PEI) were synthesized via a chemical reaction of isocyanate and hydroxyl group. By using PEI-F127-PEI block copolymers as templates combined with a sol–gel process, followed by evaporation-driven assembly
and calcination process, mesoporous TiO2 nanomaterials with different phase composition (rutile, anatase/rutile mixture, and anatase) and morphology were prepared. The prepared TiO2 samples were characterized by transmission electron microscopy (TEM), X-ray diffraction
(XRD), UV–vis diffuse reflectance spectrophotometry and N2 adsorption analysis. It is found that the mass ratio of TiCl4/PEI-F127-PEI and calcination temperature have an important influence on the phase composition and morphology of TiO2. Compared to
reported values, the transformation temperature from anatase to rutile decrease. When the mass ratio of TiCl4/PEI-F127-PEI is 2.0/1, the prepared sample exhibits the highest photocatalytic activity for the degradation of methylene blue (MB) under UV light irradiation, indicating
that the mixed anatase/rutile phase TiO2 is an efficient photodegradation catalyst of MB pollutant in wastewater treatment.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
ACS Omega
·
2021
·
IF 3.512
Jingjing Zong,Hao Peng,Xin Qing,Zhe Fan,Wenjing Xu,Xuanlong Du,Ruihua Shi,Yewei Zhang
Combination therapy such as photodynamic therapy (PDT)-enhanced chemotherapy is regarded as a promising strategy for cancer treatment. Boron-dipyrromethene (BODIPY), as close relatives of porphyrins, was widely used in PDT. However, poor water solubility, rapid metabolism by the body and lack of targeting limits its clinical application. Lenvatinib, as the first-line drug for molecular-targeted therapy of liver cancer, restricted its clinical application for its side effects. Herein, to achieve the synergy between PDT and chemotherapy, we synthesized two halogenated BODIPY, BDPBr2 and BDPCl2, which were prepared into self-assembly nanoparticles with lenvatinib, and were encapsulated with Pluronic F127 through the nanoprecipitation method, namely, LBPNPs (LBBr2 NPs and LBCl2 NPs). The fluorescence quantum yields of LBPNPs were 0.73 and 0.71, respectively. The calculated loading rates of lenvatinib for LBBr2 NPs and LBCl2 NPs were 11.8 and 10.2%, respectively. LBPNPs can be hydrolyzed under weakly acidic conditions (pH 5.0) to generate reactive oxygen species (ROS), and the release rate of lenvatinib reached 88.5 and 82.4%. Additionally, LBPNPs can be effectively taken up by Hep3B and Huh7 liver cancer cells, releasing halogenated BODIPY and lenvatinib in the acidic environment of tumor cells to enhance the targeting performance of chemotherapeutics. Compared with free lenvatinib and separate halogenated BODIPY, LBPNPs can inhibit tumor growth more effectively through pH-responsive chemo/photodynamic synergistic therapy and significantly promote the cascade of caspase apoptotic protease. This study shows that LBPNPs can be a promising nanotheranostic agent for synergetic chemo/photodynamic liver cancer therapy.
研究领域:
药学
·
纳米技术
·
癌症研究
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 48
·30250-30261
·
IF 3.108
Xin Liu,Yuebin Li,Jian He,Tingting Zhao,Changmei Chen,Haoshuang Gu,Xingguo Wang
A novel anticancer drug PFPSNT, paclitaxel (PTX)-loaded polymeric F127/P123 silica nanocapsules conjugated with TRAIL (tumor necrosis factor (TNF)-related apoptosis-inducing ligand), was designed and synthesized. Transmission electron microscopy (TEM), dynamic light scattering (DLS), UV-vis absorption and particle size analysis revealed that the synthesized PFPSNT was a spherical particle with a size of 24 nm and excellent colloidal stability. Drug loading efficiency (DL%) and encapsulation ratio (ER%) were 0.39% and 26.5%, and its cumulative release profile was linear. In vitro study showed that PFPSNT had marked cytotoxic and apoptotic activities towards HepG2 (liver cancer cell line) and CaSki (cervical cell line) cells with the IC50 values of 921.07 ng mL−1 and 236.24 ng mL−1, respectively. The antitumor efficacy of PFPSNT was evaluated by using a HepG2-xenografted BALB/c nu/nu mice model. In vivo study showed PFPSNT markedly inhibited HepG2 hepatocellular carcinoma, and its antitumor efficacy was more superior than those of TRAIL, PTX or PTX/F127/P123 silica nanocapsules. Our studies provided a novel strategy to synthesize anticancer nanodrugs with mutiple functions, and demonstrated that PFPSNT was a very effective anticancer drug. This novel nanodrug should be a promising drug for targeted cancer therapy to treat complex cancers.
研究领域:
药学
·
纳米技术
·
肿瘤学
·
药物递送系统
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 54
·49263-49271
·
IF 3.289
Shaoyu Lü,Nannan Gao,Zhen Cao,Chunmei Gao,Xiubin Xu,Xiao Bai,Chen Feng,Mingzhu Liu
Tumor-specific drug delivery is still a challenge in cancer therapy. Passive tumor targeting strategies, such as the enhanced permeability and retention (EPR) effect, cause nanocarriers to accumulate in tumors. However, this strategy can not provide specific tumor targeting. In this study, Pluronic F127 (PF127), a block copolymer which can inhibit drug efflux transporters in cancer therapy, was modified to form tumor-specific micelles with a natural polysaccharide, chondroitin sulfate (ChS), which imparts the site-specific property. A facile and efficient method based on Schiff base reaction was developed to facilitate both basic and clinical research. A series of PF127–ChS micelles with different ratios of PF127 and ChS were fabricated and evaluated in terms of size, morphology, drug loading efficiency and drug release behavior. Spherical micelles with a mean diameter of 155–241 nm were obtained. Their critical micelle concentration (CMC) was significantly reduced in contrast to PF127 micelles and their stability was enhanced. Doxorubicin (DOX) was loaded into the hydrophobic core of PF127 or adsorbed by ChS through electrostatic interactions with the negative charges of chondroitin sulfate. In vitro DOX release studies showed that DOX release from the micelles was enhanced at acidic pH values compared to physiological pH. A cytotoxicity assay (MTT) determined that the micelles possess significantly lower toxicity. Confocal microscopy and flow cytometry analysis indicated that DOX loaded micelles could efficiently release DOX inside cells by specific cellular uptake. These outcomes revealed that PF127–ChS micelles could be exploited as carriers for anti-tumor drugs for site-specific therapy of solid tumors.
研究领域:
药剂学
·
癌症治疗
·
药物递送系统
·
聚合物化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Yuhan Zheng,Feiyang Sun,Siyi Liu,Gang Wang,Huan Chen,Yongxin Guo,Xiufeng Wang,Maia Lia Escobar Bonora,Sitong Zhang,Yanli Li,Guang Chen
Background: The production of D-lactic acid (D-LA) from non-detoxified corn stover hydrolysate is hindered by substrate-mediated inhibition and low cell utilization times. In this study, we developed a novel temperature-sensitive hydrogel, F127-IEA, for efficient D-LA production using a cell-recycle batch fermentation process.Results: F127-IEA exhibited a porous structure with an average pore size of approximately 1 μm, facilitating the formation of stable Lactobacillus bulgaricus clusters within the gel matrix. It also maintains excellent mechanical properties. It also maintains excellent mechanical properties. F127-IEA immobilized Lactobacillus bulgaricus T15 (F127-IEA-T15) can be used in cell-recycle fermentation for over 150 days from glucose and 50 days from corn stover hydrolysate, achieving high production rates of D-LA from glucose (2.71 ± 0.85 g/L h) and corn stover hydrolysate (1.29 ± 0.39 g/L h). F127-IEA-T15 enhanced D-LA production by adsorbing and blocking toxic substances present in corn stover hydrolysate that are detrimental to cellular activity.Conclusions: The newly developed hydrogels in this study provide a robust platform for large-scale extraction of D-LA from non-detoxified corn stover.
研究领域:
微生物学
·
生物工程
·
发酵工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）