Analytical Methods
·
2019
·Vol. 12
·期 1
·104-111
·
IF 2.378
Jianmin Wang,Wei Yang,Qiling Peng,Daobin Han,Liangsheng Kong,Lu Fan,Min Zhao,Shijia Ding
Rapid and simple detection of carbapenem-resistant Enterobacteriaceae (CRE) is important to manage infection and prevent the spread of these bacteria. In this work, we utilize pH response to detect CRE in urine rapidly. By using vancomycin-modified Fe3O4@Au nanoparticles (Fe3O4@Au@VanNPs), CRE in urine were enriched and separated efficiently via vancomycin–cell wall interaction. More importantly, carbapenemases released via CRE lysis were detected using a pH metre which responded to hydrogen ions generated from imipenem hydrolysis. Thus, the concentration of CRE in urine was detected via the carbapenemase hydrolysis reaction. The proposed method demonstrated a good linear relationship in the range of 104–108 cfu mL−1 for CRE with the limit of detection estimated to be 1.0 × 103 cfu mL−1. More importantly, this method successfully detected clinically isolated CRE strains in urine samples within 3.5 h. Thus, this biosensing strategy might have the potential for practical application in the rapid detection of various resistant bacteria.
研究领域:
微生物学
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医学检验
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纳米技术
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高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2021
·Vol. 32
·期 2
·478-485
·
IF 3.543
Abdallah Rusul Mohammed,Al-Haddad Raad M. S.
Core–shell nanoparticles (CSNPs) have attracted attention in biomedical applications as they have highly useful materials with modified characteristics, such as high stability, dispersibility, higher permeability to certain target cells and reduction in consumption of precious materials. Synthesis of core–multishell nanoparticles with suitable sizes, structural characteristics and absorption using simple methods continues to be a challenge. In this study, \({\text{F}\text{e}}_{3}{\text{O}}_{4}@\text{A}\text{u} @{\text{S}\text{i}\text{O}}_{2}\) CSNPs were synthesized in three stages to control their size and the potential for tuning their properties. Field emission scanning electron microscope images confirmed that \({\text{F}\text{e}}_{3}{\text{O}}_{4}@\text{A}\text{u} @{\text{S}\text{i}\text{O}}_{2}\) CSNPs have a small particle size of about 22.5 nm, average crystalline size in X-ray diffractometer analysis was 22.8 nm, stability was about − 49.1 mV, and synthesis with magnetic and optical properties improved their biocompatibility. Treatment of CAL-51 and HBL-100 cell lines by \({\text{F}\text{e}}_{3}{\text{O}}_{4}@\text{A}\text{u} @{\text{S}\text{i}\text{O}}_{2}\) CSNPs under NIR laser and alternating magnetic field generated enough heat to increase cell death.
研究领域:
纳米医学
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生物材料
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肿瘤学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Congming Sun,Weiru Dong,Junxiang Peng,Xiaoliang Wan,Zhenli Sun,Dehao Li,Suhua Wang
Increasingly serious uranium pollution necessitates a new method for rapid qualitative and quantitative uranium detection. Some methods based on fluorescence spectroscopy and surface-enhanced Raman scattering (SERS) have been reported to detect uranyl ions (UO 2 2+ ), the main species of uranium. However, various factors affect the fluorescence selectivity and SERS quantitative analysis. Herein we report on a composite material for a dual-mode fluorescence–SERS detection protocol for simultaneous quantitative and qualitative analysis of UO 2 2+ , based on a layer-by-layer assembly strategy, combining the advantages of the two complementary techniques. The composite material consists of a Fe 3 O 4 @SiO 2 core coated by gold nanoparticles (FA) via electrostatic adsorption. The CdTe quantum dots (CdTe QDs) outside the FA are linked with amide bonds to form a satellite Fe 3 O 4 -Au@CdTe nanostructure (FA-CdTe). The fluorescence signal of FA-CdTe was selectively quenched by UO 2 2+ , with a detection limit as low as 1.2 nM; the material was also used as a SERS substrate to detect the Raman mode of uranyl at 828 cm −1 . Furthermore, this method has been applied for UO 2 2+ detection in seawater, river water, and tap water, demonstrating the broad application prospects and providing novel development concepts.
研究领域:
环境科学
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分析化学
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纳米技术
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材料科学
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高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Ting Zhou,Min Fan,Ruiyun You,Yudong Lu,Luqiang Huang,Yunchao Xu,Shangyuan Feng,Yang Wu,Huiying Shen,Lanjin Zhu
We have successfully prepared a highly sensitive sandwich nanosensor combined Fe 3 O 4 and [email protected] @Ag nanorods for histamine detection based on surface-enhanced Raman spectroscopy (SERS). The Fe 3 O 4 beads with –COOH served as a capture part to enrich histamine. The [email protected] @Ag core-shell nanorods functionalized with Nalpha,Nalpha-Bis(carboxymethyl)- l -lysine (AB-NTA) were then used to connect with the imidazolyl group of histamine, simultaneously the internal standard 4-aminothiophenol (4-ATP) in the core-shell structure was used as the SERS signal. PLS regression model based on concentration range 10 −3 -10 −8 mol/L showed a linear trend with R 2 = 0.9907. Our new approach can quickly and reliably determine histamine in fish sample and RAW264.7 cell lysates. This protocol for histamine extraction and SERS analysis enables the development of ultra-sensitive method for histamine detection.
研究领域:
分析化学
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纳米技术
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食品科学
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生物化学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Haixia Hu,Mingqin Wu,Liping Yao,Huiling Deng,Jiang Hu,Yukun Huang,Xiao Yang,Lijun Wang,Xianggui Chen
Recently, aptamer-enhanced nanozyme activity sensing platforms have gained considerable attention in the bioanalytical and diagnostic fields due to their simplified operations, rapid speed of analysis and visual observation convenience. However, the ability of an aptamer to enhance nanozyme activity has thus far been restricted by oligonucleotide type and length, in turn resulting in the typically high limit of detection (LOD) of aptamer-enhanced nanozyme activity sensing platforms. In this study, a novel cytosine-rich single-stranded DNA (C-rich ssDNA) was designed to overcome these drawbacks and, thereby, significantly enhance the peroxidase-like activity of Fe 3 O 4 @Au nanoparticles (NPs). The ability of this C-rich ssDNA to enhance the peroxidase-like activity of Fe 3 O 4 @Au NPs was shown to be 30% more effective than aptamers. To verify this outstanding performance, a C-rich ssDNA-enhanced Fe 3 O 4 @Au NPs activity sensing platform was developed for the detection of Salmonella Typhimurium ( S. Typhimurium). Under optimum conditions, the novel colorimetric method realized quantitative S. Typhimurium determination ranging from 2.8 × 10 1 to 2.8 × 10 6 CFU/mL, with an ultralow detection limit of 1.8 CFU/mL. Additionally, the feasibility of the proposed colorimetric method was validated in spiked lettuce and milk, with LODs of 2.7 and 3.2 CFU/mL, respectively. These results indicate the significant potential of this novel colorimetric method for effective S . Typhimurium detection in food samples.
研究领域:
生物分析化学
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纳米技术
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食品安全
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微生物学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2018
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IF 8.173
Shuli Lv,Jinliang Sheng,Shiyi Zhao,Mingchao Liu,Lihua Chen
It is a novel competitive challenge for electrochemical biosensor to directly, rapidly and ultrasensitively detect the disease markers in the whole serum due to biofouling caused by the complexity of actual samples. In this paper, poly (ethylene glycol) (PEG) and hyaluronic acid (HA) were utilized to modify Fe 3 O 4 @Au nanoparticles (NPs). Based on the successfully preparation and characterization of Fe 3 O 4 @ [email protected] @HA NPs with TEM , SEM, XRD, FTIR and EDS , respectively, a novel immunosensor of brucellosis with high selectivity , sensitivity and almost perfect protein-resistant properties in various external environments, especially, in complex biological systems was fabricated. More importantly, this immunosensor is capable of assaying brucellosis antibody in 100% serum without suffering from any significant biological interference. In addition, a wide linear response range from 10 –15 g mL −1 to 10 –11 g mL −1 towards antibody in 100% serum and a low limit of detection (LOD) of 0.36 fg mL −1 (3σ, n = 13) are demonstrated, which indicates that this immunosensor has a promising potential in clinical diagnosis.
研究领域:
生物传感器
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纳米技术
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分析化学
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临床诊断
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DOI
PubMed
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2019
·Vol. 125
·期 5
·1-10
·
IF 1.784
Guo Huiling,Wang Wenjing,Zhou Fengzhen
In this study, core–shell Fe 3 O 4 @Au nanoparticles with good chemical stability and tunable particle size were synthesized via Au–S bonding, which overcomes the challenges encountered for the reported preparation of Fe 3 O 4 @Au. The surface of nanoparticles was investigated by TEM, XRD, XPS, FT-IR, VSM and DLS which characterized the size, morphology, chemical lattice, elemental analysis, functional groups, magnetic strength, and size distribution. The results showed that Fe 3 O 4 @Au/NTA-Ni 2+ magnetic nanocomposites were spherical with an average diameter of 469.5 nm, and superparamagnetic with saturation magnetization of 7 emu/g. The magnetic nanocomposites were directly utilized for one-step purification of His-tagged proteins from Description Escherichia coli lysate. The as-separated proteins were qualitatively validated by gel analysis and quantitatively measured by UV–visible spectroscopy. The binding capacity of Fe 3 O 4 @Au/NTA-Ni 2+ towards His-tagged proteins was 48.3 mg/g. The target proteins were separated with high purity and separation efficiency up to 96.6%. Their specificity and enrichment ability were maintained during six cycles of adsorption–elution operations. The advantages of this novel nanoparticle can open a window for the multifunction of nanomaterials and their biological application.
研究领域:
生物化学
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纳米技术
·
蛋白质工程
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Wenjuan Shang,Hui Xin,Xiandeng Hou,Li Wu,Lan Wu
In this work, a boric-acid-modified Fe3O4@Au@BA-MOF composite material as a multifunctional SERS substrate was ingeniously constructed for detecting both pathogens and antibiotics as well as photothermally inactivating the pathogens. Through improving the dispersity and stability of gold nanoparticles (Au NPs), leveraging the specificity of boric acid (BA) groups in recognizing cis-diol structures, and the ability of SERS technology to provide unique fingerprint spectra of targets, the sensitive and stable detection of pathogens and antibiotics was achieved. Compared with Au NPs and Fe3O4@Au, the SERS enhancement factor of Fe3O4@Au@BA-MOF was 4.31 × 106, which was about 400 times and 16 times higher than the former two, respectively. Among the existing work, the limit of detection for pathogens was lower or comparable, and it exhibited good stability, maintaining consistent performance for 23 days. Additionally, this substrate achieved efficient photothermal inactivation of pathogens under both near-infrared light and natural light excitation. Within 8 min of near-infrared light irradiation, the bactericidal rates for Staphylococcus aureus and Escherichia coli reach 100% and 99.3%, respectively.
研究领域:
分析化学
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生物医学工程
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
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2014
·
IF 2.2
Xiaofang Shen,Zhaoqiang Ge,Yuehong Pang
Gold-coated magnetic core@shell nanoparticles , which exhibit magneto-optical properties, not only enhance the chemical stability of core and biocompatibility of surface, but also provide a combination of multimodal imaging and therapeutics. The conjugation of these tiny nanoparticles with specific biomolecules allows researchers to target the desired location. In this paper, superparamagnetic Fe 3 O 4 @Au nanoparticles were synthesized and functionalized with the azide group on the surface by formation of self-assembled monolayers. Folate (FA) molecules, non-immunogenic target ligands for cancer cells, are conjugated with alkyne and then immobilized on the azide-terminated Fe 3 O 4 @Au nanoparticles through copper(I)-catalyzed azide-alkyne cycloaddition (click reaction). Myelogenous leukemia K562 cells were used as a folate receptor (FR) model, which can be targeted and extracted by magnetic field after interaction with the Fe 3 O 4 @Au–FA nanoparticles.
研究领域:
生物医学工程
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纳米医学
·
肿瘤学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 7
·496
·
IF 5.4
Yao Haixu,Wang Yan,Shi Jincheng,Gao Xinzhuo,Yang Xueqiong,Liu Mengyuan,Li Yuxin,Jin Yingyu,Zhou Qin,Cui Feiyun
Real-time, synchronous profiling of key inflammatory cytokines such as IFN-γ, IL-6 and TNF-α is critical for understanding and managing dysregulated immune responses, including cytokine storms associated with severe infections. However, conventional immunoassays are not readily compatible with the requirements of rapid, multiplexed analysis in point-of-care settings. Herein, we report a multiplexed electrochemical biosensing platform based on biomimetic interfacial encoding using Fe 3 O 4 @Au nanoplatforms (BIENs). The core sensing element consists of extracellular vesicle-mimetic nanocomposites functionalized with cell membranes overexpressing specific cytokine receptors, enabling native-like molecular recognition. This encoded biomimetic interface affords high-affinity and selective detection of IFN-γ, IL-6 and TNF-α with wide linear ranges (0.1 ~ 1000 pg/mL) and low detection limits (0.043 ~ 0.159 pg/mL). The sensor exhibits excellent selectivity, operational stability, and reproducibility, and maintains high accuracy in serum samples with minimal matrix interference. Beyond analytical performance, this work establishes a receptor-mimetic interfacial encoding strategy for multiplexed biosensing, offering a versatile and scalable paradigm for the development of next-generation intelligent biointerfaces and dynamic immune monitoring systems. Graphical
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CHEMPHYSCHEM
·
2012
·Vol. 13
·期 18
·4142-4147
·
IF 3.412
Xinmei Zhao,Narayan S. Hosmane,Aiguo Wu
Graphical Link up: ortho -Phenylenediamine (PDA) acts as a linker in the deposition of gold nanoparticles on the surfaces of Fe 3 O 4 microspheres. After coating with PDA, the morphology of the microspheres changes significantly to nanoparticle clusters, and this is ideal for the deposition of the gold nanoparticles. The resulting Fe 3 O 4 /Au nanocomposites exhibit enhanced magnetism so that they can be recovered and recycled easily. 1,2-Diaminobenzene, popularly known as ortho -phenylenediamine (PDA), is found to be a prototype spacer for the deposition of gold nanoparticles on the surfaces of Fe 3 O 4 microspheres. Upon carbonization with PDA, the morphology of the product changes significantly, and the resulting nanocomposites exhibit enhanced magnetism beyond the saturation value of Fe 3 O 4 . The Fe 3 O 4 /Au nanocomposites show good surface-enhanced Raman spectroscopy activity with a detection limit of 10 −15 M .
研究领域:
纳米技术
·
材料科学
·
化学
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DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 46
·17148-17162
·
IF 4
Yunqi Xu,Kang Wang,Yi Zhu,Jing Wang,Dazheng Ci,Min Sang,Qunling Fang,Huaxia Deng,Xinglong Gong,Ken Cham-Fai Leung,Shouhu Xuan
The global health crisis of bacterial resistance to antibiotics requires innovative antibacterial strategies. One promising solution is the exploitation of multifunctional nanoplatforms based on non-resistant antibacterial mechanisms. This work reports a novel Fe3O4@Au/polydopamine (PDA) nanodurian with excellent photothermal-magnetomechanic synergistic antibacterial effects. The one-step formed Au/PDA hybrid shell provides good photothermal properties and spiky surfaces for enhanced magnetomechanic effects. Upon near-infrared (NIR) irradiation, the Fe3O4@Au/PDA nanodurian (200 μg mL−1) achieved nearly 100% antibacterial effect against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The efficiency of photothermal antimicrobial activity was further enhanced by the application of a rotating magnetic field (RMF), with the sterilization efficiency being increased by up to more than a half compared to the action alone. Interestingly, the size of the nanodurian has a significant impact on the synergistic sterilization effect, with larger particles showing a superior performance due to stronger chain-like structures in the magnetic field. Finally, the Fe3O4@Au/PDA nanodurian also demonstrates effective biofilm removal, with larger particles exhibiting the best eradication effect under the photothermal-magnetomechanic treatment. Overall, this magnetic field enhanced photothermal antibacterial strategy provides a promising broad-spectrum antimicrobial solution to combat bacterial infections. Thus, it possesses great potential in future nanomedicine and pollution treatment.
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