Scientific Reports
·
2016
·Vol. 6
·期 1
·1-11
·
IF 5.228
Liu Xiumei,Xu Wenjuan,Mao Liaoyuan,Zhang Chao,Yan Peifang,Xu Zhanwei,Zhang Z. Conrad
Cellulosic ethanol production from lignocellulosic biomass offers a sustainable solution for transition from fossil based fuels to renewable alternatives. However, a few long-standing technical challenges remain to be addressed in the development of an economically viable fermentation process from lignocellulose. Such challenges include the needs to improve yeast tolerance to toxic inhibitory compounds and to achieve high fermentation efficiency with minimum detoxification steps after a simple biomass pretreatment. Here we report an in-situ detoxification strategy by PEG exo-protection of an industrial dry yeast (starch-base). The exo-protected yeast cells displayed remarkably boosted vitality with high tolerance to toxic inhibitory compounds and with largely improved ethanol productivity from crude hydrolysate derived from a pretreated lignocellulose. The PEG chemical exo-protection makes the industrial S . cerevisiae yeast directly applicable for the production of cellulosic ethanol with substantially improved productivity and yield, without of the need to use genetically modified microorganisms.
研究领域:
生物技术
·
可再生能源
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Yifei Wang,Kangfu Zhou,Chuanjun Ye,Yazhuo Shang,Feifei Wang
Microemulsions are thermodynamically stable and homogeneously dispersed systems formed spontaneously by water, oil, surfactants, and co-surfactants at specific proportions under specific conditions. Microemulsions can not only dissolve certain active ingredients to achieve protection and slow release, but also effectively promote their transdermal absorption, further improving the bioavailability due to the nanoscale of the emulsion droplets. In this study, microemulsions formulated with surfactant polyethylene glycol (PEG) fatty acid glycerides were prepared and the effects of oil property, the amphiphilicity of surfactant, the types of co-surfactants, the main/co-surfactant mixing ratio ( K m ) as well as the temperature, pH, ionic strength of the systems on the phase behavior of the studied system were discussed in the framework of the pseudo-ternary phase diagram. The results indicate that lower polarity oils are more likely to form microemulsions constructed by PEG fatty acid glycerides. The formation of microemulsion depends much on the amphiphilicity of surfactant; PEG fatty acid glycerides with higher hydrophile-lipophile balance (HLB) facilitate the formation of microemulsions. The shorter the chain length of co-surfactant (alcohols), the easier it is for the formation of microemulsion. For alcohols with a certain number of carbon chains, the fewer the hydroxyl groups, the easier it is to form microemulsions. This study identifies an optimal K m of 2:1. Based on the fact that PEG fatty acid glycerides are nonionic surfactants, the prepared microemulsions have a good tolerance to pH fluctuations (pH 4–9) and keep stable within a wide temperature range, which enable the microemulsions to be applied in variable environments. The higher concentration salt inhibits microemulsion formation in some degree though the lower concentration salt has no obvious effect on the formation of microemulsion. Furthermore, the types of microemulsions in the single-phase region were further identified, and the microstructure of microemulsions formulated with PEG fatty acid glycerides was investigated by transmission electron microscopy (TEM). Typical spherical oil-in-water (O/W) microemulsions, uniformly distributed water-in-oil (W/O) microemulsions, and the characteristic network structure of bicontinuous microemulsions were observed. On this basis, the water-soluble nicotinamide was singled out to study the transdermal behavior of substance in oil-in-water microemulsion and in traditional emulsion. The results indicate that the substance in microemulsion have better transdermal property compared with conventional emulsion. The present work provides not only fundamental information for the preparation of microemulsions formulated with PEG fatty acid glycerides but also the reference for its application in practice.
研究领域:
药剂学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Hexiang Sun,Zachary Magnuson,Wenwen He,Weijie Zhang,Harsh Vardhan,Xiaolong Han,Gaohong He,Shengqian Ma
The desulfurization property of conventional mixed matrix membranes (MMMs) cannot meet the necessary demand due to particles aggregation and interface defects. Here, we put forward a layer-by-layer (LBL) approach to make a novel PEG@ZIF-8/poly(vinylidene difluoride)(PVDF) composite membrane for pervaporation desulfurization. In this way, a ZIF-8 layer is covered on the surface of the PVDF porous membrane via an in situ growth method. Then, a PEG layer is covered on the ZIF-8 layer by a casting method. Compared with pristine PEG membranes, the separation performance of the ZIF-8@PEG/PVDF nanocomposite membrane increased significantly. This can be attributed to the homogeneous ZIF-8 particle layer and better compatibility between the poly(ethylene glycol) (PEG) matrix and ZIF-8 particles. The membrane achieves a maximum total flux of 3.08 kg·m–2·h–1 at the third in situ growth cycles of ZIF-8 particles and a maximum sulfur enrichment factor of 7.6 at the sixth in situ growth cycles of ZIF-8 particles.
研究领域:
化学工程
·
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Chaoyang Zhang,Conghao Gao,Shumin Yang,Xin He,Yue Chen,Xiao Qin,Yulin Tang
Chloramphenicol (CAP) is used extensively in industry and daily life, but its abuse has seriously affected the environment and public health. In this paper, a new composite PbO 2 electrode was obtained through the modification Sm and polyethylene glycol (PEG), and an electrocatalytic oxidation technology of CAP degradation was investigated. The results showed that the catalytic degradation ability and industrial service life of the PEG-Sm-PbO 2 composite electrode were significantly enhanced. Co-doping inhibited the growth of grains, resulting in the formation of refined pyramidal grains on the surface of the electrode, which increased the number of active spots. The industrial service life of the modified electrode was improved by 87.0%. In addition, the degradation effect under different conditions and mechanism of CAP were also explored. The optimal conditions for CAP degradation were explored, at which time the CAP degradation rate reached 99.1%. The degradation process was in accordance with the primary reaction kinetics, and the apparent rate constant of CAP at the PEG-Sm-PbO 2 electrode was raised by 57.1% in comparison with the unmodified electrode, indicating that the modification facilitated the degradation of CAP in the electrode. Finally, two possible CAP degradation pathways were deduced. The results will provide technical support and a theoretical basis for the degradation of persistent organic pollutants.
研究领域:
环境科学
·
电化学工程
·
污染控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2024
·Vol. 16
·期 9
·1248
·
IF 4.7
Pengzhi Bi,Xiuzhong Zhu,Li Tian,Jinbang Han,Wanbin Zhang,Tong Wang
In recent years, a great deal of work has been devoted to the development of thermoresponsive polymers that can be made into new types of smart materials. In this paper, a branched polymer, HTPB-g-(PNIPAM/PEG), with polyolefin chain segments as the backbone and having polyethylene glycol (PEG) and poly(N-isopropylacrylamide) (PNIPAM) as side chains was synthesized by ATRP and click reactions using N3-HTPB-Br as the macroinitiator. This initiator was designed and synthesized using hydroxyl-terminated polybutadiene (HTPB) as the substrate. The temperature-responsive behavior of the branched polymer was investigated. The lower critical solution temperature (LCST) of the branched polymer was determined by ultraviolet and visible spectrophotometry (UV-vis) and was found to be 35.2 °C. The relationship between the diameter size of micelles and temperature was determined by dynamic light scattering (DLS). It was found that the diameter size changed at 36 °C, which was nearly consistent with the result obtained by UV-vis. The results of the study indicate that HTPB-g-(PNIPAM/PEG) is a temperature-responsive polymer. At room temperature, the polymer can self-assemble into composite micelles, with the main chain as the core and the branched chain as the shell. When the temperature was increased beyond LCST, the polyolefin main chain along with the PNIPAM branched chain assembled to form the nucleus, and the PEG branched chain constituted the shell.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2021
·Vol. 38
·期 7
·2100062
·
IF 3.31
Pengfei Yang,Zhihuan Zhao,Shasha Yang,Jianying Lin,Jimin Fan,Bing Zhang
In this study, a chemotherapy-photothermal synergistic anti-tumor system is constructed. Both W 18 O 49 @R-C and PEG-W 18 O 49 @R-C synthesized by hydrothermal method show the potential of photothermal therapy (PTT). The structure of reflux-carbon is bubble-like spherical and W 18 O 49 @R-C obtained by hydrothermal synthesis will cause bubble collapse due to the formation of crystal W 18 O 49 ; thus, the particle size decreases sharply. Furthermore, the photothermal stability of PEG-W 18 O 49 @R-C is higher than that of W 18 O 49 @R-C, and ζ-potential measurement indicates that PEG-W 18 O 49 @R-C has excellent dispersion characteristics. The test of drug loading and drug release performance show that PEG-modified W 18 O 49 @R-C has superior performance on drug loading amount and release capacity. In the synergistic anti-tumor process, the cancer cell viability after co-incubating with PEG-W 18 O 49 @R-C+DOX . HCl (with 808nm) is only 16.6%. These results indicate that PEG-W 18 O 49 @R-C has potential in the treatment of cancer by a combination of PTT and chemotherapy.
研究领域:
肿瘤学
·
材料科学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Miaomiao Ma,Yuhong Qi,Chuanjun Gu,Zhanping Zhang
Revealing the adsorption behavior of proteins on amphiphilic polymers is an interesting study. For the first time, the adsorption behavior of bovine serum albumin (BSA) protein on the surface of polyethylene glycol polystyrene (PEG-PS) amphiphilic polymer membrane is developed by molecular dynamics (MD) simulations and experiments. We found that PEG-PS has the ability to resist BSA protein adsorption. The adsorption of BSA on its surface undergoes a process of "rotation-preadsorption-desorption-final adsorption". After adsorption, the helical structure of BSA underwent "tight-loose-unwinding" changes. The transition from PEG chain helical structure to free coil structure is a key factor leading to the adsorption and desorption of BSA. Furthermore, the hydrophobic force of PS is the main driving force for BSA adsorption. These studies can inspire the design of amphiphilic materials to realize protein antifouling or transportation materials.
研究领域:
生物物理学
·
材料科学
·
蛋白质工程
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2014
·
IF 8.312
Jing Bai,Yuwei Liu,Xiue Jiang
The synergistic therapy, the combination of photothermal therapy and chemotherapy, has become a potential treatment in the battles with cancer. Here, we developed a synergistic therapy tool that based on CuS nanoparticles-decorated graphene oxide functionalized with polyethylene glycol (PEG-GO/CuS) for cervical cancer treatment. The as-synthesized PEG-GO/CuS nanocomposites with excellent biocompatibility was revealed to have high storage capacity for anticancer drug of doxorubicin (Dox) and high photothermal conversion efficiency, and were effectively employed for the ablation of tumor. In addition, the therapeutic efficacy of Dox-loaded PEG-GO/CuS (PEG-GO/CuS/Dox) nanocomposites was evaluated in vitro and in vivo for cervical cancer therapy. In vitro cell cytotoxicity tests of PEG-GO/CuS/Dox demonstrate about 1.3 and 2.7-fold toxicity than PEG-GO/CuS and free Dox under 5 min irradiation with NIR laser at 1.0 W/cm 2 , owing to both PEG-GO/CuS-mediated photothermal ablation and cytotoxicity of light-triggered Dox release. In mouse models, mouse cervical tumor growth was found to be significantly inhibited by the chemo-photothermal effect of PEG-GO/CuS/Dox nanocomposites, resulting in effective tumor reduction. Overall, compared with chemotherapy or photothermal therapy alone, the combined treatment demonstrates better therapeutic efficacy of cancer in vitro and in vivo . These findings highlight the promise of the highly versatile multifunctional nanoparticles in biomedical application .
研究领域:
生物医学工程
·
肿瘤学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
·
2025
·
IF 8.9
Miaomiao Ma,Yuhong Qi,Qi’an Chen,Zhanping Zhang
Biofouling of underwater equipment caused by marine organisms is a major problem in the marine industry. Although hydrogel exhibits excellent antifouling properties, its mechanical performance greatly limits its practical application. In this work, a suitable marine antifouling hydrogel (PSM) was designed utilizing a polyethylene glycol–modified MXene (Ti 3 C 2 T x -PEG) toughening mechanism and a silica loaded with nitrogen-doped carbon dots (SiO 2 @N-CDs) reinforcement strategy to enhance the durability of the hydrogel. The PSM hydrogel exhibited a fracture strength of up to 1.62 MPa, elongation of 1734 %, and toughness of 17.7 MJ/m 3 . Furthermore, the PSM hydrogel exhibited excellent photothermal antibacterial properties, with antibacterial rates against E. coli and S. aureus reaching 100%. After 180 days of immersion in seawater, the PSM hydrogel morphology remained stable. Because of its long-term durability and effective antifouling properties, our PSM hydrogel can be developed for use in marine ship coatings.
研究领域:
材料科学
·
生物医学工程
·
海洋科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2011
·Vol. 21
·期 38
·14639-14645
·
IF 5.101
Xiao-Jun Cai,Hai-Qing Dong,Wen-Juan Xia,Hui-Yun Wen,Xue-Quan Li,Jin-Hai Yu,Yong-Yong Li,Dong-Lu Shi
Engineered PEG-detachable catiomers were developed as non-viral gene vectors to detach the PEG layers responsive to the intracellular reducing environment. These catiomers were found to exhibit high DNA binding ability and reduced cytotoxicity, as evidenced in agarose gel electrophoresis and MTT assays. The size of the mPEG-SS-PLL/DNA complexes was around 100 nm with a regular spherical shape, as observed under transmission electron microscopy (TEM). The complexes were stably dispersed in an aqueous medium with 10% serum. However, fast aggregation was observed in the presence of 10 mM glutathione (GSH) due to detachment of the PEG segment via disulfide cleavage. These complexes showed high transfection efficiency in 293T and Hela cells under optimized conditions. The experimental results indicated that the mPEG-SS-PLL catiomers may have promising potential as a non-viral gene vector.
研究领域:
生物化学
·
分子生物学
·
基因治疗
✓ 已验证
产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2016
·
IF 3.349
Xiangrong Chen,Bingxue Tang,Jianquan Luo,Yinhua Wan
Polysulfone (PSF) membranes were prepared by nonsolvent-induced phase separation (NIPS) method using PSF-b-PEG copolymer as pore formation additive. The effects of copolymer composition, hydrophilic chain length and copolymer dosage on membrane morphologies, permeability, stability and biocompatibility were systematically discussed in order to determine the role of the additive. The results suggest that the copolymer composition and dosage play an important role in pore formation. The increase of PEG content in the copolymer results in larger pore size and narrower pore size distribution . The modified membranes show average surface pore sizes in the range of 11.4–15.2 nm, which increase by 14–52% compared to that of the pristine PSF membrane. With increase of copolymer dosage, the pore size increases first and then decreases. The hydrophilic chain length has no significant influence on the pore size and size distribution. The results of pure water flux and rejection agree with the variation of pore size. With increasing the copolymer dosage, the static adsorption of protein and platelet to the membrane is suppressed and thus the antifouling ability is improved. The PSF-b-PEG copolymer outperforms the common water soluble additive (i.e. PVP) in terms of the better pore forming ability and membrane stability, implying that the PSF-b-PEG copolymer would be an alternative to PVP in membrane preparation.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
npj Biofilms and Microbiomes
·
2025
·Vol. 11
·期 1
·1-16
·
IF 9.2
Mwangi Kevin H.,Qu Yue,Hu Peilun,Nagayasu Toshitatsu,Liu Jia-Feng,Wang Xiumei
Critical bone defects, exacerbated by infections, pose significant challenges to bone healing and homeostasis, necessitating the development of dual-functional biomimetics that combine anti-infective and reparative capabilities. The EGaIn holds promise across various disciplines, though its interactions with microbial cells require further elucidation. This investigation evaluates the antimicrobial efficacy of PEG-EGaIn nanocapsules against a spectrum of bacterial, employing electron microscopy. Constructs containing 1.5% PEG-EGaIn hinder biofilm-producing bacteria, while 3% concentrations amplify the biocidal effect. Furthermore, the nanocapsules promoted osteogenic differentiation rBMSCs, evidenced by enhanced mineralization and upregulation of key osteogenic genes. In addressing large bone defects, PEG-EGaIn-Col-Ap-lamellar and ethanolic-mediated Col-Ap-lamellar constructs serve as potential solutions for bone resorption mitigation and osteo-angiogenesis. Bone-remodeling were validated through μ-CT and histomorphometry confirming no evidence of chronic inflammation or fibrosis. In this study, PEG-EGaIn nanocapsules emerge as potent biocide and bone repair, underscoring their potential in combating antibiotic resistance and enhancing bone healing.
研究领域:
微生物学
·
生物医学工程
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）