Materials Today Communications
·
2026
·
IF 4.9
Yong Wu,Jin Gui Li,Cheng Ming Zhang,Zi Fu Cai,Cun Chen,Zhi Xing Cao,Rui Zeng
Background The relationship between structural variations of polysaccharides and their physiological roles remains unclear. Monosaccharide composition influences their antioxidant activity, potentially linking to blood glucose regulation and microbiome modulation. Methods This study focused on Rosa roxburghii Tratt polysaccharide (RP) and Brasenia schreberi polysaccharide (BP). Fractions BSP-40, BSP-60, RRTP-40, and RRTP-60 were prepared, and their structure-function relationships were analyzed via in vitro assays and animal intervention experiments. Results RRTP-40/60 were rich in galacturonic acid and glucose, while BSP-40/60 mainly contained glucose and mannose. RRTP-60 and BSP-40 exhibited a Congo red binding pattern characteristic of a triple helix, unlike RRTP-40 and BSP-60. Polysaccharide administration reduced fasting blood glucose by 24.44% (RP group), 27.56% (RP-BP group), and 29.65% (BP group), decreased triglyceride (TG) and malondialdehyde (MDA) levels, improved hepatic cell morphology, and reduced inflammatory markers. Specifically, the abundance of Muribaculaceae has significantly increased. This bacterial family has been reported to exhibit increased abundance in health interventions and is believed to exert a positive influence on host metabolism through pathways such as the production of short-chain fatty acids, while harmful Lachnospiraceae decreased. Monosaccharide composition correlated significantly with intestinal microbiota. Conclusion The monosaccharide composition and conformational features affect their regulatory effects on blood sugar and the gut microbiota.
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高影响因子
PEST MANAGEMENT SCIENCE
·
2025
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IF 3.8
Handinghong Zheng,Jiaohong Li,Zhenxiang Guo,Guy Smagghe,Shunhua Gui,Xiaomao Wu,Xiangsheng Chen
BACKGROUND Rosa roxburghii produces a distinctive edible fruit renowned for its high vitamin C content and medicinal properties. Leaf brown spot is a newly identified fungal disease in R. roxburghii , causing considerable production losses in southwest China. RESULTS In this study, the pathogenic fungus causing brown spot in R. roxburghii was identified as Neopestalotiopsis saprophytica (CXCHB-2). Carvacrol, among seven selected phytocompounds, demonstrated strong antifungal activity against N. saprophytica with a 50% effective concentration (EC 50 ) of 19.72 mg L −1 . The mechanisms underlying the antifungal activity of carvacrol against N. saprophytica were investigated, and it showed that carvacrol disrupted N. saprophytica mycelium growth by inhibiting mycelial matrix, and compromising cell wall and membrane integrity. Moreover, the in vivo antifungal assays confirmed that carvacrol could inhibit the growth of N. saprophytica in R. roxburghii leaves with excellent therapeutic and protective effects (76.61% and 70.63%, respectively). Further investigations indicated that this phytocompound induced the upregulation of 24 differentially expressed genes related to the leaf hormone signal transduction of R. roxburghii , enhancing its resistance to N. saprophytica . CONCLUSION Carvacrol exhibited potent antifungal activity against N. saprophytica , causing leaf brown spot in R. roxburghii by disrupting its cell walls and membranes. Moreover, carvacrol could enhance the resistance of R. roxburghii leaves to N. saprophytica . Findings of this study suggested that carvacrol may serve as a promising plant-derived fungicide for controlling leaf brown spot in R. roxburghii leaves, providing a serviceable reference for its application in the agroforestry system. © 2025 Society of Chemical Industry.
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PubMed
高影响因子
Nanomaterials
·
2025
·Vol. 15
·期 19
·1513
·
IF 4.3
Dan Liu,Shilan Feng
This study usedRosa roxburghiiTratt. crude extract (RR) as a reducing, stabilizing, and modifying agent for the green synthesis of gold nanoparticles (RR-AuNPs) via the one-pot method for the first time and established a novel colorimetric sensor for detecting tiopronin. Initially, RR-AuNPs with a uniform particle size and stable dispersion were prepared using the reducing property of RR. Upon the introduction of tiopronin, the drug binds to the surface of RR-AuNPs through Au-S bonds and hydrogen bonds, inducing a significant aggregation of RR-AuNPs. The absorbance of the RR-AuNP solution exhibited a linear relationship with the tiopronin concentration in the range of 0.17 μM to 16.67 μM (y = 1.9157 − 0.0972x), with a detection limit of 0.19 μM. The colorimetric sensor was successfully applied to detect tiopronin in urine samples. Compared with other detection methods, this approach is simple to operate and has a high sensitivity, a wide linear range, and a low detection limit.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Sibu Wang,Junyu Wang,Jun Zhang,Xingjie Wu,Qianqian Guo,Yu'e Wang,Ling Tao,Xiangchun Shen,Ying Chen
Precancerous lesions of gastric carcinoma (PLGC) are the most important stage in the development of gastric cancer, accompanied by significant oxidative stress and inflammatory response. Rosa roxburghii extract (RRE) has unique advantages in anti-PLGC due to its multi-component, high antioxidant and anti-inflammatory activities. However, the astringency and instability of RRE in the digestive tract seriously hinder its clinical application. Herein, we report a chitosan-based food-grade Pickering emulsion (PE) for loading RRE to block unpleasant taste, improve stability, and promote the entry of RRE into gastric epithelial cells through the gastric adhesion of chitosan, thereby enhancing preventive and therapeutic effects against PLGC. This Pickering emulsion is constructed as a water-in-oil (W/O) emulsion stabilized by the food-grade nanoparticles composed of soybean protein isolate (SPI) and chitosan (CS) through electrostatic interaction (defined as RRE@PE). The experimental results showed that RRE@PE performed better efficacy against PLGC than RRE by scavenging or inhibiting reactive oxygen species generation and reducing inflammatory cytokines. This Pickering emulsion enhances the application potential of RRE and is expected to be used for the treatment of clinical patients with PLGC.
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高影响因子
Journal of Agriculture and Food Research
·
2025
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IF 6.2
Xiaoli Zhou,Zhicheng Qin,Yan Chen,YaPing Wang,Bin Du,Dong Lin,Lingshuai Meng
Dietary fiber (DF) is a crucial part of a healthy diet. However, the impacts of bound polyphenols (BPs) on the diversity of gut microbiota and the production of metabolites—such as short-chain fatty acids (SCFAs)—as regulated by DF during in vitro digestion and fermentation remain largely unclear. Here, we evaluated the effects of BP removal on the in vitro digestion and fermentation of DF derived from Rosa roxburghii Tratt. (RRT)—a medicinal and edible fruit. Using DF rich in BPs (RRT-DF) and dephenolic DF (RRT-dP-DF), changes in polyphenol content (PC) and antioxidant activity during digestion and fermentation were evaluated alongside fermentation parameters (pH and SCFAs), gut microbiota structure, and metabolome profiles. Polyphenol release from RRT-DF was limited during gastrointestinal digestion (4–6%) but increased significantly during fermentation (25–27%). The fermentation broth of RRT-DF exhibited a significantly higher antioxidant capacity than that of RRT-dP-DF. RRT-DF also displayed favorable prebiotic properties; BPs enhanced DF-mediated SCFA production, modulated gut bacterial composition, and promoted the metabolism of lipids/lipid molecules and organic acids and their derivatives. Overall, these findings advance understanding of the potential role of BPs in regulating the in vitro digestion and fermentation of RRT-DF.
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高影响因子
Acta Cardiologica Sinica
·
2020
·
IF 0
Pin-Hsin Wu,Samuel Chieng-Haw Han,Meng-Hsiu Wu
Background Rosa roxburghii Tratt fruit is extensively used as a medicinal and edible resource in China due to its unique bioactivities. In this research, we aimed to characterize its phenolic acid composition and investigate the potential hypolipidemic effect of this plant in a rat model of hyperlipidemia. Methods We evaluated the effects of hydroalcoholic extract of Rosa roxburghii Tratt fruit (HRT) on serum lipids, body weight, activities of lipoprotein metabolism and antioxidant enzymes, and gene expression of lipid metabolism in hyperlipidemic rats. Results HRT significantly reduced body weight gain and decreased serum and liver lipid levels in the hyperlipidemic rats. In addition, HRT treatment improved the activities of antioxidant enzymes, lipoprotein lipase, and hepatic lipase, downregulated the mRNA and protein expressions of sterol regulatory element-binding protein 1c and acetyl CoA carboxylase, and upregulated the mRNA and protein expressions of peroxisome proliferator-activated receptor α and low-density lipoprotein receptor in hepatic tissue. Conclusions The results showed that Rosa roxburghii Tratt fruit is rich in phenolic acids, and that it exerted lipid lowering effects in the hyperlipidemic rats.
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PubMed
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
·
2026
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IF 3.3
Zhangrui Luo,Jiaxing Yu,Jiaohong Li,Xiaomao Wu
Rosa roxburghii Tratt., a vitamin C-rich edible and medicinal fruit native to southwest China, plays a vital role in poverty alleviation and economic development in mountainous regions. In this study, Neofusicoccum parvum was identified for the first time as a pathogen causing fruit rot in R. roxburghii . Koch's postulates were fulfilled by re-isolating the fungus from artificially inoculated fruits, confirming its pathogenicity. Morphological and molecular analyses further validated the pathogen's identity. The optimal growth conditions for N. parvum were determined to be 28–30 °C and pH 5, with D-fructose and NH 4 Cl as the preferred carbon and nitrogen sources, respectively. Continuous light exposure was found to maximize mycelial growth. Among six tested plant extracts, osthol, Scutellariae Radix extract, and matrine exhibited notable antifungal activity, with osthol showing the highest efficacy (EC 50 = 30.57 μg/mL). Field trials demonstrated that 1% osthol emulsion in water (EW), applied at 300–500-fold dilution, achieved 60.68%–77.57% control efficacy against fruit rot after 21 d of the second application. This performance was comparable to the chemical fungicide 10% difenoconazole water dispersible granule (WG) (67.58%–81.04% efficacy at 5000–7000-fold dilution). This study provides a scientific basis for the biocontrol of N. parvum -induced fruit rot disease in R. roxburghii .
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高影响因子
Food & Function
·
2018
·Vol. 9
·期 7
·3974-3985
·
IF 3.289
Lei Wang,Chun Chen,Bin Zhang,Qiang Huang,Xiong Fu,Chao Li
A novel acidic polysaccharide (RTFP-3) was isolated and purified from Rosa roxburghii Tratt fruits. Structural analyses revealed that RTFP-3 is a homogeneous polysaccharide with a molecular weight of 67.2 kDa and mainly consists of arabinose (37.20%), galactose (34.40%), glucose (10.02%) and fucose (18.30%). The structural chain of RTFP-3 was proven to be composed of →6)-α-D-Galp-(1→, →4)-α-D-GalpA-(1→, →5)-α-L-Araf-(1→, →4)-β-D-Glcp-(1→, β-D-Glcp-(1→ and →3,4)-β-L-Fucp-(1→ residues by periodate oxidation–Smith degradation, methylation, and 1D- and 2D-NMR analyses. Bioassay results showed that RTFP-3 exerted favorable mixed type inhibitory activities against α-glucosidase, and its half inhibitory concentration (IC50) value was determined as 4.15 mg mL−1. RTFP-3 showed static type quenching of the intrinsic fluorescence of α-glucosidase, during which the binding process induced the conformational change of α-glucosidase. These results suggest that RTFP-3 has the potential to be a novel α-glucosidase inhibitory agent for use in functional foods.
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高影响因子
Foods
·
2024
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IF 4.7
Qiuqiu Zhang,Sha Wu,Qinghua Dai,Peng Hu,Guangjing Chen
Drying conditions significantly impact the compositions and microstructures of polysaccharides, leading to various effects on their chemical characteristics and bioactivities. The objective of this study was to investigate how different industrial drying techniques, i.e., hot air drying, infrared drying, microwave vacuum drying, and freeze drying, affect the structural properties and biological activities of polysaccharides extracted from Rosa roxburghii Tratt fruit (RRTP). Results revealed that these drying methods significantly altered the extraction yield, molecular weights, monosaccharide ratios, contents of uronic acid and total sugars, gelling properties, particle sizes, thermal stability, and microstructures of RRTPs. However, the monosaccharide composition and functional groups of polysaccharides remained consistent across the different drying techniques. Biological activity assays demonstrated that RRTPs, particularly those processed through microwave vacuum drying (MVD-RRTP), exhibited excellent anti-linoleic acid oxidation, robust anti-glycosylation effects, and significant α-glucosidase inhibition in vitro. The outcomes of this research demonstrate that microwave vacuum drying serves as an effective pre-extraction drying method for RRTPs, enhancing their biological activities. This technique is particularly advantageous for preparing RRTPs intended for use in functional foods and pharmaceuticals, optimizing their health-promoting properties for industrial applications.
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Food Bioscience
·
2025
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IF 5.9
Ting-Ting Tu,Xiao-Hui Yuan,Yu-Heng Mao,Yan-Chun Wang,Zheng-Hao Chen,Lei Wang,You Luo,Chun-Xiao Wang,Shu-Yi Qiu,Ang-Xin Song
This study aimed to investigated the structural and physicochemical properties of two pectic polysaccharide fractions (RTFP1-2 & RTFP2) isolated from Rosa roxburghii Tratt fruit by anion-exchange and size-exclusion chromatography purification, as well as their protection on Bifidobacterium spp. against antibiotic damage. RTFP1-2 had a relatively lower molecular weight of 1.12 × 10 5 Da and RTFP2 had a higher molecular weight of 5.06 × 10 5 Da. Chemical composition analysis revealed that both RTFP1-2 and RTFP2 consisted of galacturonic acid (53.13 %, 64.73 %), galactose (27.26 %, 20.04 %), arabinose (3.57 %, 2.94 %), rhamnose (2.32 %, 1.67 %) and glucose (13.73 %, 10.62 %), respectively. These two pectic polysaccharides mainly contained homogalacturonan and rhamnogalacturonan I domain. RTFP1-2 and RTFP2 were resistant to in vitro gastrointestinal digestion with no more than 18 % (w/w) hydrolysis. Minimum inhibitory and bactericidal concentration (MIC and MBC) determination showed RTFPs could notably promote bifidobacterial survivals against three commonly used antibiotics with MIC increasing to ≥ 64 μg/mL and MBC to ≥ 512 μg/mL. The protection attributed to the viscous layers formed by the RTFPs surrounding the bacterial cells to prevent antibiotics from accessing to the bacteria. The viscous RTFP1-2 and RTFP2 also promoted the cohesion among bacterial cells and enhanced bacterial biofilm formation. The results suggested that RTFPs could be potential prebiotic additives used alone or supplemented with probiotic bacteria.
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Foods
·
2024
·Vol. 13
·期 18
·2893
·
IF 4.7
Su Xu,Junyi Deng,Siyao Wu,Qiang Fei,Dong Lin,Haijiang Chen,Guangcan Tao,Lingshuai Meng,Yan Hu,Fengwei Ma
Rosa roxburghii (R. roxburghii), native to the southwest provinces of China, is a fruit crop of important economic value in Guizhou Province. However, the changes in fruit quality and flavor duringR. roxburghiifruit ripening have remained unknown. Here, this study investigated the changes of seven active components and volatile organic compounds (VOCs) during the ripening of theR. roxburghiifruit at five different ripening stages including 45, 65, 75, 90, and 105 days after anthesis. The results indicated that during the ripening process, the levels of total acid, vitamin C, and soluble sugar significantly increased (p< 0.05), while the levels of total flavonoids, superoxide dismutase (SOD), and soluble tannin significantly decreased (p< 0.05). Additionally, the content of total phenol exhibited a trend of first decreasing significantly and then increasing significantly (p< 0.05). A total of 145 VOCs were detected by HS-SPME-GC-MS at five mature stages, primarily consisting of aldehydes, alcohols, esters, and alkenes. AsR. roxburghiimatured, both the diversity and total quantity of VOCs in the fruit increased, with a notable rise in the contents of acids, ketones, and alkenes. By calculating the ROAV values of these VOCs, 53 key substances were identified, which included aromas such as fruit, citrus, green, caramel, grass, flower, sweet, soap, wood, and fat notes. The aromas of citrus, caramel, sweet, and wood were predominantly concentrated in the later stages ofR. roxburghiifruit ripening. Cluster heatmap analysis revealed distinct distribution patterns of VOCs across five different maturity stages, serving as characteristic chemical fingerprints for each stage. Notably, stages IV and V were primarily characterized by a dominance of alkenes. OPLS-DA analysis categorized the ripening process ofR. roxburghiifruit into three segments: the first segment encompassed the initial three stages (I, II, and III), the second segment corresponded to the fourth stage (IV), and the third segment pertained to the fifth stage (V). Following the variable importance in projection (VIP) > 1 criterion, a total of 30 key differential VOCs were identified across the five stages, predominantly comprising ester compounds, which significantly influenced the aroma profiles ofR. roxburghiifruit. By integrating the VIP > 1 and ROAV > 1 criteria, 21 differential VOCs were further identified as key contributors to the aroma changes inR. roxburghiifruit during the ripening process. This study provided data on the changes in quality and aroma ofR. roxburghiifruit during ripening and laid the foundation for the investigation of the mechanism of compound accumulation during ripening.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Zhenting Zhang,Sixiu Zhong,Ruihua Zhang,Qibing Zeng,Pinggui Lei,Wenjin Qiu,Wang Chen,Daoping Wang,Xinjing Wei,Qun Huang,Ting Hu,Peng Luo
Skeletal fluorosis is closely associated with inflammation and metabolic disorders caused by excessive fluoride exposure. Rosa roxburghii Tratt juice (RRTJ), a natural dietary supplement, has been widely used to manage diseases associated with inflammatory damage and metabolic disorders. However, it is largely unknown if RRTJ can mitigate fluoride-induced bone metabolism disorders and the underlying mechanisms. In this study, we demonstrated that RRTJ supplementation significantly improved fluoride exposure-induced bone metabolism disorders, enhanced anti-inflammatory responses, increased short-chain fatty acid concentrations (SCFAs), strengthened the intestinal barrier, and markedly enriched beneficial bacteria, particularly Ligilactobacillus spp. Notably, we identified Rosa roxburghii Tratt polysaccharides (RRTP) as a key component of RRTJ that significantly promotes the growth of Ligilactobacillus murinus . Additionally, RRTJ effectively alleviated fluoride-induced lipid metabolism disorders. A mechanistic study revealed that RRTJ fortifies intestinal barrier function via activating intestinal G-protein-coupled receptor 43 (GPR43) and inhibiting NF-κB signaling pathways, leading to ameliorating bone metabolism disorders. These findings reveal the unique mechanism of RRTJ in balancing bone metabolism and provide new insights into the interaction between natural products and the gut microbiota, offering potential dietary strategies to mitigate fluorosis-induced bone metabolism disorders.
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高影响因子
危险品化学品经营许可证（带存储）