TALANTA
·
2025
·
IF 6.1
Mingyue Luo,Xin Xue,Kehui Zhang,Honghong Rao,Ruibin Qiang,Panpan Sun,Marcin Frankowski,Zhonghua Xue
Multi-signal sensors possess immense potential for point-of-care testing, yet their widespread adoption is hindered by reliance on complex nanomaterial synthesis or fragile enzymatic systems. Herein, we propose a “six-in-one” multi-mode sensor for rapid iodide ion (I - ) detection, leveraging only three commercially available reagents: chloroplatinic acid (H 2 PtCl 6 ), hexadecyl trimethylammonium bromide (CTAB), and 3,3’,5,5’-tetramethylbenzidine (TMB). The core innovation lies in a dynamic “on-off-on” oxidation triggered by target I - ions as follows: H 2 PtCl 6 with strong oxidative capacity directly oxidizes TMB to its two-electron product (TMB 2+ ) (“On” State). CTAB coordinates with H 2 PtCl 6 thereby blocking its surface and completely inhibiting TMB oxidation (“Off” State). I - selectively displaces CTAB via competitive coordination, partially restoring H 2 PtCl 6 ’s oxidation activity to generate the one-electron product (TMBox) (“Reactivation” State). Remarkably, the resulting TMBox exhibits six distinct signal outputs including visual and fluorescent color, absorbance, fluorescence, temperature, and electrical current. Compared to conventional single- or dual-mode methods, our six-mode approach improves reliability of detection results. This “magic cube” signals enables dual-mode qualitative analysis and four-mode quantitative detection, achieving a nanomolar-level detection limit for I - detection. Critically, multi-mode sensor operates via one-step mixing without requiring nanomaterials, enzymes, or specialized equipment. We further demonstrate its utility in detecting I - in biological (urine, serum), food (iodized salt) and environmental (Yellow River water, tap water) samples with recovery rates of 96.3% - 110.0%. Statistical analysis demonstrated excellent reproducibility with relative standard deviations <3.1% across repeated measurements. This work redefines the paradigm of multi-mode sensing, offering a cost-effective, field-deployable solution for environmental monitoring and clinical diagnostics.
研究领域:
分析化学
·
医学诊断
·
环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Pengjuan Ni,Yujing Sun,Haichao Dai,Jingting Hu,Shu Jiang,Yilin Wang,Zhen Li,Zhuang Li
In this work, a facile approach to sensitively detect sulfide ions is developed. The proposed method is based on the findings that silver ions could oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to induce a blue color and an absorption peak centered at 652 nm. The introduction of sulfide ions leads to the formation of Ag 2 S nanoparticles which act as catalyst to enhance the oxidation capacity of silver ions, resulting in a blue color burning and increase of the absorbance at 652 nm. Based on the above facts, a facile method is developed to qualitatively and quantitatively detect sulfide ions by naked eyes and UV–vis spectroscopy, respectively. The color changes of the detection system in the presence of nanomolar level of sulfide ions could be distinguished by naked-eyes, demonstrating the high sensitivity of the method. Highly sensitive detection of sulfide ions can be achieved in the range of 1.0 × 10 −9 M to 8.0 × 10 −8 M, with a detection limit of 0.2 nM. In addition, this method shows excellent selectivity toward sulfide ions detection and has been successfully applied to detect sulfide ions in tap water and lake water samples. Moreover, this method is simple without the preparation of nanomaterials and the need of complex readout instruments.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Xiaoming Ma,Yue Lin,Longhua Guo,Bin Qiu,Guonan Chen,Huang-hao Yang,Zhenyu Lin
3, 3′, 5, 5′-tetramethylbenzidine (TMB) has been widely used as a chromogenic substrate for colorimetric immunoassays . Normally, the colorless TMB is oxidized into yellow TMB 2+ (in acidic solution) to indicate the presence of the target molecules. However, this kind of monochromic intensity changes seriously confine the accuracy of visual inspection. Herein, we demonstrate for the first time that TMB 2+ can quantitatively and efficiently etch gold nanorods (AuNRs). The addition of AuNRs into a solution containing different amount of TMB 2+ generates vivid color responses as colorful as a rainbow, and the etching process can be finished within 90 s. As a result, the exact concentration of TMB 2+ can be easily distinguished with the naked eyes by the corresponding solution color. Based on this finding, we incorporate AuNRs into the well-developed, commercially available horseradish peroxidase (HRP)-TMB immunoassay system, so that it can be utilized for semiquantitative detection of a broad range of disease biomarkers with the naked eyes (termed ‘NEQ-IA’). Carcinoembryonic antigen (CEA) and Prostate specific antigen (PSA) had been chosen as example targets to test the feasibility of the proposed biosensor. The results showed good accordance with the conventional methods. Because no sophisticated apparatus but human eyes are used as the readout, the proposed NEQ-IA could be a good supplementary to current state-of-the-art immunoassay methods for those applications that require the use of portable and affordable devices, for example, for the detection of disease biomarkers at home and in the field.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING RESEARCH & DESIGN
·
2021
·
IF 3.739
Han Wang,Shulan Wang,Jingqiao Zhang,Han Wang,Hui Li,Yi Zhao
Several mesostructured cellular foams (MCFs) were prepared and modified for NO adsorption. TMB, NH 4 F and n -heptane were used as swelling agents, and APTES was used as the modified agent. The morphological structure, chemical composition, thermostability were tested to study the effects of the preparation process on the characterizations of materials. NO adsorption ability of the materials were studied and discussed. NH 4 F and n -heptane had better pore swelling effects compared to TMB, however adding TMB together with NH 4 F and n -heptane could not enlarge the pore structure further. The MCFs materials could keep steady below 800 °C, but after grafting APTES on the surface of the materials, the thermogravimetric curve of materials began to decrease at 300 °C. The materials using NH 4 F and n -heptane as the swelling agents, and modified by APTES had better NO adsorption ability, and the selectivity of the materials were decided by the molecular polarity of the adsorbates. After the modification, the hydrophobic effect of the samples could increase because of the alkyl groups in APTES. The adsorption capacity of the materials could keep steady for ten times without the coexisting component, H 2 O, CO 2 and SO 2 all had negative effects on the regeneration of the materials.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Si Gao,Haiyan Zhao,Yixuan Yang,Xincai Xiao,Dan Zhao
Carbon dots (CDs) with both fluorescence and nanozyme activity are regarded as excellent probe for developing fluorescence/colorimetric dual-mode sensing systems. The sensitive, selective, rapid and portable detection of the natural polyphenol antioxidant tannic acid (TA) is of great significance. This study reports the employment of green-emitting carbon dots with peroxidase-like activity (Fe-CDs) and red-emitting carbon dots (R-CDs) as dual probes to establish a fluorescence/colorimetric dual-mode sensing biosensor to realize the detection of tannic acid. With the presence of hydrogen peroxide, Fe-CDs catalyze the oxidation of TMB to produce a blue product (OX-TMB), causing the color solution change from red to blue. Meanwhile, the fluorescence of the sensing platform weakens with blueshift in emission wavelength because of the inner filter effect (IFE). Because TA can effectively inhibit the Fe-CDs/TMB chromogenic reaction, its presence leads to the recovery of both solution color and fluorescence intensity. This sensing platform exhibits good selectivity, anti-interference ability to various antioxidants. The colorimetric and fluorescence sensors demonstrate excellent linear relationships within the concentration ranges of 8–600 nM and 8–1000 nM, respectively, with detection limits as low as 4.54 nM and 7.47 nM. A portable hydrogel-based detection platform was then developed, enabling multi-color colorimetric/fluorescence dual-channel visual detection either by the naked eye or with smartphone assistance. The system has been successfully applied to detection TA in real samples with recovery rates at 95.51–107.87%. This research provides a novel dual-signal verification strategy for the highly sensitive and selective detection of natural antioxidants and offers a new approach for the real-time analysis of active components in complex matrices.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Optical Materials
·
2025
·
IF 3.8
Yujiao Jiang,Ziyi Ye,Mengling Yu,Zhengxing Gong,Xinfeng Zhang
With the widespread use of personal electronic devices, excessive blue light exposure that causes retinal cell damage and skin hyperpigmentation has attracted increasing attention. However, an effective indicator for real-time in-house monitoring of blue light is still lacking. In this study, we developed a screen-attachable visual hydrogel indicator for facile in-house monitoring of blue light. The indicator is fabricated by encapsulating both a blue light-responsive photosensitizer (fluorescein) and 3,3′,5,5′-tetramethylbenzidine (TMB) within an agarose hydrogel. Upon exposure to blue light, the photosensitizer generates reactive oxygen species (ROS), which oxidize colorless TMB to blue oxTMB. The proximity of fluorescein and TMB within the hydrogel nanopores significantly facilitates the oxidation reaction. Hence, using a hydrogel as the reaction carrier not only makes the indicator screen-attachable but also greatly enhances its sensitivity. The color change of TMB is good linearly with the blue light doses in the range of 0.15–1.2 J/cm2 with a detection limit about 0.082 J/cm2, much lower than the threshold (0.75 J/cm2) for oxidative eye stress. Green and red light from the electronic products caused no interference for the monitoring of blue light. It was successfully applied for monitoring the cumulative dose of blue light emitted from the three types of electronic products (i.e., laptops, tablets, and light-emitting diode (LED) lamps) by simply attaching the indicator on the screen or on the desk. This hydrogel indicator, with the merits of screen-attach ability, facile use, and high sensitivity, is quite appealing for in-house monitoring of blue light from electronic products.
研究领域:
光学传感
·
材料科学
·
生物医学工程
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Yijuan Long,Xiliang Wang,Dongjun Shen,Huzhi Zheng
It was found that reduced state carbon dots (r-CDs) possessed intrinsic peroxidase-like activity, and could catalytically oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to produce a color reaction. The effects of temperature, pH, incubating time and the concentration of H 2 O 2 and TMB on catalytic activity of r-CDs were investigated. Finally we calculated the kinetic constant was ca. 0.00729 min −1 and applied r-CDs to glucose sensing by coupling glucose oxidase . As low as 2 µM H 2 O 2 could be detected with a linear range from 0.010 to 0.40 mM via this method. This study offered a simple, sensitive, and high selectivity method for glucose determination even in serum.
研究领域:
生物化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Xiangyu Jiang,Fenghua Geng,Yongxiang Wang,Jinhua Liu,Peng Qu,Maotian Xu
Quantitative fluorescence turn-on and colorimetric detection of glutathione (GSH) with rapid speed, low cost have attained much attention. Herein, we developed a sensitive fluorescence turn-on and colorimetric sensor for GSH based on the inner-filter effect (IFE), which is the first time to select oxTMB and TMPyP as the IFE absorber and fluorophore pair, respectively. The absorption band of oxTMB matches well with the emission band of TMPyP in the IFE-based fluorescent assay. In the absence of GSH, the absorption peak of oxTMB at 652 nm significantly overlaps with the emission of TMPyP, resulting in the efficient IFE and inhibition of the fluorescence of TMPyP. In the presence of GSH, the absorption intensity at 652 nm decreases, generating the recovery of the fluorescence of TMPyP. Therefore, this approach is demonstrated to be a novel candidate for detection of GSH, with high sensitivity and selectivity. The linear dynamic range for the concentrations of GSH is between 0.1 μM to 20 μM along with a limit of detection (LOD) of about 30 nM (calculated LOD as 3 σ /slope). Finally, this novel sensor was successfully applied for GSH detection in fetal calf serum , and satisfactory recovery was achieved.
研究领域:
分析化学
·
生物传感
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Ruimin Li,Mingming Zhen,Mirong Guan,Daiqin Chen,Guoqiang Zhang,Jiechao Ge,Ping Gong,Chunru Wang,Chunying Shu
C 60 -carboxyfullerene, C 60 [C(COOH) 2 ] 2 , has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H 2 O 2 to produce a blue color reaction. Kinetic studies further indicate that C 60 [C(COOH) 2 ] 2 has an even higher affinity to TMB than that of the natural enzyme , horseradish peroxidase (HRP). C 60 [C(COOH) 2 ] 2 /glucose oxidase (GO x )/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over a range of 1.0–40 μM with a limit of detection 0.5 μM (3 σ /slope). Moreover, this sensitive and selective sensor can be successfully applied for the quantitative determination of glucose in human serum. The results indicate that it is a simple, cheap, convenient, highly selective, sensitive, and easy handling colorimetric assay .
研究领域:
生物化学
·
医学检测
·
纳米材料
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yu Han,Ting Zhang,Lin Zhu,Jiawei Liu,Jianjun Liao,Long Wu
Artificial antioxidants (AOs), including BHT, BHA, PG, and TBHQ, are extensively utilized in food, pharmaceutical, and other industries to prevent oxidation and prolong the shelf life of products. However, excessive intake of these AOs may bring about potential health risks, such as allergic reactions or interference with normal physiological functions. As a result, there is a pressing need for sensitive, simple, and simultaneous detection methods for AOs. Electrochemical sensors, which usually involves modification of electrodes with complex procedures and delicate operations, has its practicality in real-world applications somewhat restricted. Herein, we developed a versatile detection system based on the catalytic activity of Pt Au nanoflowers (Pt Au NF) in the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Specifically, the Pt Au NF/TMB reaction system produces a distinct blue color, which progressively fades with AOs. This colorimetric response offers a straightforward, naked-eye readout for AOs detection. Moreover, the Pt Au NF/TMB system can also be employed for localized electrochemical detection using the redox activity of TMB. By simply mixing Pt Au NF and TMB in a one-step procedure, we established two complementary detection modes: naked-eye readout and localized electrochemical detection. The differential pulse voltammetry (DPV) mode displayed satisfactory analytical performance towards BHT, BHA, PG and TBHQ, with linear ranges of 0.1 to 200 mg/L and detection limits of 0.063, 0.048, 0.025 and 0.016 mg/L (3σ/slope), respectively. The integration of “one-step mixing” and “one-drop detection”, therefore, greatly reduces operation complexity, providing an accurate and efficient platform for AOs detection, as well as a valuable tactic for combined detection in food samples.
研究领域:
分析化学
·
纳米技术
·
食品安全
·
生物传感
✓ 已验证
产品关联
DOI
高影响因子
INORGANIC CHEMISTRY
·
2021
·
IF 5.165
Ling Yu,Hua Li,Wei Huang,Haili Yu,Yi He
Imaging the catalytic activity at the single-particle level can greatly promote the screening and rational design of highly efficient nanozymes, but conventional techniques are based on ensemble analysis. Here, we present a new absorption microscopy for in situ visualizing oxidase-mimicking activity of single MnOOH nanotubes. The particle with a size more than 700 nm roughly equally scatters all wavelengths of visible light via Mie scattering, and the scattering light is collected by dark-field optical microscopy. When the particles absorb a single color of the scattering light, each individual nanoparticle shows its complementary color, enabling a form of absorption microscopy that we name Mie scattering-based absorption microscopy. We find that MnOOH nanotubes can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate polyTMB nanowires at their tips. There are multiple active sites on the surface of the individual nanotube, and the nanozyme activity shows a large heterogeneity as well as pH-dependent characteristic.
研究领域:
纳米技术
·
催化
·
显微技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Micromachines
·
2021
·Vol. 12
·期 9
·1099
·
IF 2.891
Ning Jiang,Chuang Zhang,Meng Li,Shuai Li,Zhili Hao,Zhengqiang Li,Zhuofu Wu,Chen Li
The assessment of glutathione (GSH) levels is associated with early diagnostics and pathological analysis for various disorders. Among all kinds of techniques for detecting GSH, the colorimetric assay relying on the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) catalyzed by many nanomaterials with peroxidase-like activity attracts increasing attention owing to its outstanding merits, such as high sensitivity and high selectivity. However, the aggregation between the nanomaterials severely hinders the entrance of TMB into the “active site” of these peroxidase mimics. To address this problem, the D-amino acid incorporated nanoflowers possessing peroxidase-like activity with a diameter of 10–15 μm, TMB and H2O2were employed to establish the detection system for determining the level of glutathione. The larger diameter size of the hybrid nanoflowers substantially averts the aggregation between them. The results confirm that the hybrid nanoflowers detection system presents a low limit of detection, wide linear range, perfect selectivity, good storage stability and desired operational stability for the detection of GSH relying on the intrinsic peroxidase-like activity and favorable mechanical stability of the hybrid nanoflowers, indicating that the hybrid nanoflowers detection system has tremendous application potential in clinical diagnosis and treatment.Keywords:amino acid-incorporated nanoflowers;glutathione;peroxidase-like activity
研究领域:
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）