PROCESS BIOCHEMISTRY
·
2018
·
IF 2.616
Jinsha Huang,Wei Zhuang,Lei Ge,Kai Wang,Zhenfu Wang,Huanqing Niu,Jinglan Wu,Chenjie Zhu,Yong Chen,Hanjie Ying
Surface amine modification could not only improve the microenvironment near the active sites of enzyme, but also enhance the multi-point chemical crosslinking between the enzyme and carrier. The linear structured polymer of ε-poly - l - lysine (EPL) is an ideal donor of amino with much more exposed on the surface for enzyme attachment. Analysis of the result of dynamic light scattering (DLS) and circular dichroism (CD) demonstrated the favorable electrostatic interactions and negligible impact on the conformation of enzymes, Glucose Oxidase and Catalase (GOx&CAT). Titration and dissociation curve together with Zeta-potential characterization revealed that enzymes (EPL@GOx&CAT) under the protection of EPL had more stable structure and better activity and stability in acidic reaction environment. Meanwhile, the activity recovery of immobilized EPL@GOx&CAT increased to 1.56 times higher than that of GOx&CAT, and the acid resistance was improved by 1.44 times with the optimum pH shifting to acidic by 0.50 unit. The substrate affinity was raised with the decrease of K m from 5.98 to 4.35 mg mL −1 . Thus, the gluconic acid production in low pH system could be increased by pH-engineering of the enzyme microenvironment via conjugation with positively charged polyelectrolyte.
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高影响因子
CATALYSIS LETTERS
·
2018
·Vol. 148
·期 7
·2019-2029
·
IF 2.911
Liu Anqiu,Huang Zhong,Wang Xiaochen
Abstract A new method has been developed for the oxidation of glucose into gluconic acid over Pd/C catalysts under the room temperature and atmospheric pressure. The carbon support was prepared by the hydrothermal treatment of renewable glucose, thus contained abundant active oxygen species. The Pd/C catalyst showed high catalytic activity towards the oxidation of glucose into gluconic acid. A 100% glucose conversion and a 98% gluconic acid yield were attained within 2 h at 0.1 MPa and room temperature. Structural analysis showed that the Pd paricle sizes of the catalysts were in the range of 10.1–16.2 nm influenced by the loading of Pd. The structure/size study showed that the catalyst with optimal particle size of 10.9 nm exhibited the highest TOF (0.2388 mol glucose mol Pd /s). The catalyst showed no significant loss of activity after recycled for four times. Graphical Abstract
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高影响因子
Catalysis Science & Technology
·
2020
·Vol. 10
·期 7
·2231-2241
·
IF 5.721
Jie Yin,Quanquan Zhang,Changjun Yang,Bingguang Zhang,Kejian Deng
Selective oxidation of glucose into gluconic acid and glucaric acid with molecular oxygen under mild conditions in only water as the solvent remains a challenge. To achieve highly selective oxidation of glucose to gluconic acid and glucaric acid, a new TiO2/HPW/CoPz composite photocatalyst was prepared through modifying TiO2 with HPW (phosphotungstic acid) and CoPz (cobalt tetra(2-hydroxymethyl-1,4-dithiin)porphyrazine), which was well characterized by Raman spectroscopy, X-ray diffraction (XRD), UV-vis diffuse reflectance spectroscopy (UV-vis DRS), inductively coupled plasma-mass spectrometry (ICP-MS) and scanning electron microscopy energy-dispersive spectrometry (SEM-EDS). Remarkably, it was found that this composite photocatalyst can efficiently catalyze oxidation of glucose to gluconic acid and glucaric acid with atmospheric oxygen at high selectivity in water under simulated sunlight irradiation. For example, the total selectivity for both acids can reach up to 80.4% with 22.2% glucose conversion under the optimized conditions. The excellent photocatalytic performance of the TiO2/HPW/CoPz composite was attributed to the synergistic effect between the components. To elucidate the roles of HPW and CoPz in the photocatalytic process, a series of experiments including surface photocurrent, electrochemical impedance, electron spin resonance (ESR) spectroscopy, zeta potential and adsorption behavior measurements were performed. It has been demonstrated that the presence of HPW can effectively reduce the adsorption capacity of gluconic acid and glucaric acid on the surface of the catalyst and suppress their further oxidation, resulting in a high selectivity to both acids. Meanwhile the presence of CoPz can improve the separation efficiency of photogenerated charges and accelerate the production of active species, facilitating the conversion of glucose without sacrificing the acid selectivity.
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高影响因子
APPLIED ENERGY
·
2016
·
IF 5.746
Ke Wang,Zhongyun Zhou,Pengfei Zhao,Zeguang Yin,Zhen Su,Ji Sun
A unique gluconic acid treatment coupled with a carbon coating process was used to synthesize Li 4 SiO 4 ceramics (GAC-Li 4 SiO 4 ) with superior performance for high-temperature CO 2 capture. The effects of different synthetic methods, carbonization temperatures, acid sources and acid contents on the performance of the sorbent were studied and characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), nitrogen absorption and thermogravimetric analyses. After the gluconic acid treatment and carbon coating, the GAC-Li 4 SiO 4 had a small crystal size, hollow morphology and enhanced pore features. Significant improvements in CO 2 sorption performance (i.e., low absorption temperature, fast absorption rate, and high capacity) were thus obtained. Moreover, the carbonization temperature, acid content and acid type greatly affected the morphology and chemisorption properties of the Li 4 SiO 4 sorbents. Under the optimized synthetic conditions, the maximum CO 2 absorption capacity was 34.7% after isothermal absorption at 665 °C for 10 min. This sorbent also maintained good cyclic properties.
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高影响因子
AQUACULTURE NUTRITION
·
2021
·Vol. 27
·期 3
·897-907
·
IF 3.497
Fan Luo,Zhihuan Fu,Mingjie Wang,Zhiwei Ke,Manna Wang,WenXiong Wang,Murtaza Hasan,Xugang Shu
Zn is one of the essential elements to maintain normal fish metabolic process (including growth, development and reproduction), which have different chemical status in nature including inorganic, organic and nano-sized. In this work, the efficacy of gluconic acid zinc (Glu-Zn), nano zinc oxide (Nano ZnO), zinc sulphate (ZnSO 4 ) on the growth performance, tissue mineral elements deposition, antioxidant activity and immune response of the tilapia fish Nile tilapia ( Oreochromis niloticus ) was evaluated. Three experimental diets were prepared by adding different forms of Zn sources (ZnSO 4 as a control group) with same Zn concentration (40 mg/kg) and used to feed fish for 60 days. The biological trial results displayed that the growth performance of Nile tilapia has not obviously affected by different Zn form source supplement. However, Glu-Zn and Nano ZnO supplements have significantly stimulated the Zn and Cu absorption deposition efficiency in the fish tissues as compared to ZnSO 4 group. Besides, there was a significant increase in Cu-Zn superoxide dismutase (35.03 U/mgprot), alkaline phosphatase (79.34 U/mgprot), aspartate aminotransferase (23.42 U/L), alanine aminotransferase (18.45 U/L), glutathione peroxidase (171.24 U/mgprot) and fatty acid synthase (11.55 ng/mL) content in Glu-Zn treatment. Additionally, Glu-Zn supply significantly increased serum lysozyme (9.91 U/mL), immunoglobulin M (0.48 g/L), complement 3 (0.74 g/L) and complement 4 (0.95 g/L) activity of Nile tilapia. Also, compared to ZnSO 4 diet, lysozyme (11.44 U/mL) and complement 4 (0.63 g/L) content were obviously higher in fish fed Nano Zn diet. These findings revealed that supplementation with Glu-Zn as a Zn source in feed is better than ZnSO 4 for improving tissue mineralization, antioxidant stress, alleviate lipid peroxidation and immunity response of Nile tilapia. Meantime, Nano ZnO also played a positive role in the tissue mineralization and immunity response of Nile tilapia.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Shuo Liu,Hongli Chao,Meishan Guo,Ruifang Wang,Dongyu Gu,Yi Wang,Dajun He,Yi Yang
To achieve the biotransformation of cellulose into gluconic acid, a multi-enzyme reactor comprising cellulase, glucose oxidase, and catalase was constructed through artificial antibody-antigen-directed immobilization. Catechol was used as a template to prepare artificial antibodies, which were then used to modify the enzymes through a Schiff base reaction to form artificial antigens. The artificial antibody and antigen were subsequently assembled via affinity recognition to immobilize the enzymes. The immobilization yields for all three enzymes exceeded 85 %, with specific activities of 43.48 ± 1.54 U/mg, 0.88 ± 0.06 U/mg, and 18.47 ± 1.43 U/mg, respectively. The immobilized enzymes demonstrated excellent adaptability, storage stability, reusability, and regenerability. Through the modular assembly of artificial antigen-antibody, the multi-enzyme reactor facilitated the cascade conversion of cellulose to gluconic acid.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ziyi Yang,Xiaoyan Dong,Zhenfu Wang,Yan Sun
Gluconic acid is widely used in food and pharmaceutical. However, its bio-synthesis by glucose oxidase (GOx) is blocked by depletion of dissolved O 2 , reduction of pH value, and accumulation of hydrogen peroxide (H 2 O 2 ). To remove the by-products and replenish O 2 , developing a nanozyme with high catalase (CAT)-like activity and stability under acidic conditions is a prescription. Herein, we developed a manganese-based nanozyme (ps-MnO x -BSA) through a stepwise strategy and encapsulated it in Fe-doped zeolitic imidazolate framework-8 (FZ). The as-prepared ps-MnO x -BSA@FZ (MFZ) exhibited not only high stability and CAT-like activity but also exceptionally no peroxidase-like activity at pH 5.5. Thereafter, GOx@MFZ was fabricated by the immobilization of GOx on MFZ and utilized for gluconic acid synthesis with a yield of 98.3 % within 30 min. GOx@MFZ retained 92.3 % of its initial activity after six batches. This work provided a novel strategy for the design of acid-resistant CAT-like nanozyme toward sustainable gluconic acid production.
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PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2021
·
IF 8.128
Yuze Zhang,Lijuan Li,Dong Shi,Fugen Song
Epoxy resin (EP) is a polymer that is widely used in different aspects of life, but its flammability property limits its fields of applications. Most flame retardants at present cannot be applied practically in scale due to their toxicity, incompatibility in polymers, degraded mechanical property or high cost of raw materials and comprehensive preparation process. Layered double hydroxides intercalated by gluconic acid anion (GLDHs) may serve as a new approach. GLDHs with Mg/Al ratio of 3/1 and 2/1 were first coprecipitated with low-cost green reactants, MgCl 2 ·6H 2 O, AlCl 3 ·6H 2 O, NaOH and sodium gluconate. Their structures were confirmed by X-ray diffractions (XRD), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR) and elemental analysis; their thermal properties were analyzed by thermo gravimetric analysis (TGA). Composites containing 40 wt.% GLDHs were easily manufactured with normal magnetic stirring without any filler precipitation. The combustion properties of the composites filled with 40 wt.% GLDHs2 are as follows: the limit oxygen index (LOI) could rise to 29.8% from 25.2% of pristine EP; UL-94 can reach V-1 level with total burning time of only 12.1 s without dropping; compared to pristine EP, the heat release rate peak (PHRR) could drop to 30% with heavy decrease in the smoke production rate and CO production rate. Dynamic mechanical thermal analysis (DMA) tests showed that the addition of 40 wt.% GLDHs had little impact on the glass transition temperature of the composites and could slightly improve their rigidity and toughness. Tensile strength of the composites filled with 40 wt.% GLDHs2 was almost close to 88% of the tensile strength of pristine EP. Above all, GLDHs with good compatibility in polymers can serve as a promising environmental friendly and low-cost flame retardant for EP and other heterochain polymers.
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PubMed
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 95
·期 4
·1116-1125
·
IF 2.659
Han Zhang,Shao-Feng Hua,Lei Zhang
BACKGROUND The objective of this research was to use a step-by-step method to prepare a novel biocatalytic system (GO-CEL-E/N-GA-GOD): -OH and -COOH on the surface of graphene oxide (GO) were used to immobilize cellulase (CEL) and glucose oxidase (GOD), respectively, which may be easier to control the loading of enzymes with different kinds, to achieve the one-pot conversion of gluconic acid from carboxymethyl cellulose (CMC). RESULTS The loadings capability of CEL and GOD on GO-CEL-E/N-GA-GOD were 49.07 ± 7.47 mg g −1 and 10.22 ± 2.03 mg g −1 , respectively. The multi-enzyme systems had shallow temperature and pH optima of 40 °C and 5.0. The kinetic constants of GO-CEL-E/N-GA-GOD (of CEL-GOD) were K m = 0.15 ± 0.02 (0.43 ± 0.09) mmol L −1 , V max = 0.18 ± 0.01 (0.21 ± 0.07) μmol L −1 s −1 , and k cat / K m = 24.12 ± 0.52 (17.74 ± 0.85) s −1 mmol −1 L. Nearly 65% of the initial activity of GO-CEL-E/N-GA-GOD could be retained after seven cycles. Notably the conversion of gluconic acid was able to reach 63.82 ± 8.03% within 2 h. CONCLUSION Step-by-step immobilization method made full use of different functional groups on GO, avoiding the competition for fixed sites between CEL and GOD in the immobilization processes. The results reflected the feasibility of the strategy to build bio-microsystem as CEL and GOD immobilized on GO by covalent bonds to achieve the conversion from CMC to gluconic acid in one-step. © 2019 Society of Chemical Industry
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高影响因子
GREEN CHEMISTRY
·
2014
·Vol. 16
·期 5
·2558-2565
·
IF 6.852
Fuhua Zhao,Hui Li,Yijun Jiang,Xicheng Wang,Xindong Mu
Here we report a simple and efficient approach to fabricate a new biocatalytic system by co-immobilizing multi-enzyme on chemically reduced graphene oxide (CRGO) via non-covalent bonds. The obtained artificial biocatalyst was characterized by UV/Vis, FTIR, AFM, TEM and SEM. Compared with native and graphene oxide (GO) bounded enzymes, it was found that the glucose oxidase (GOD) or glucoamylase (GA) immobilized on CRGO exhibited significantly higher enzymatic activity, due to the positive effect of the CRGO carrier. This multi-enzyme microsystem was employed as a biocatalyst to accomplish the starch-to-gluconic acid reaction in one pot, and the yield of gluconic acid could reach 82% within 2 hours. It was also proved that the stability of the multi-enzyme biocatalyst immobilized on CRGO was dramatically enhanced compared with the GO microsystem. About 85% of the activity of the artificial biocatalyst could be preserved after four cycles. These results demonstrated the feasibility of the novel strategy to construct bio-microsystems with multi-enzyme on 2D CRGO via non-covalent bonds to accomplish some complex conversions.
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高影响因子
危险品化学品经营许可证（带存储）