CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Yili Zeng,Nan Duan,Caiping Fu,Dejin Liao,Xiaolan Song,Shengming Jin,Kuixin Cui
Using tailings to prepare constructive materials is of great significance for sustainable development of mineral processing industry. In this study, the possibility of preparing tungsten tailing-based geopolymers was explored in detail. XRD , FTIR , PLM, SEM and XPS analyses were carried out to characterize the phase composition, chemical bonding, microstructure, chemical state, and interface properties of tungsten tailing-based geopolymers. Results showed tungsten tailings presented little activity using NaOH as activator, while geopolymers with 60% non-pretreatment tungsten tailing and 40% metakaolin presented a 3-day compressive strength of 8.4 MPa and 28-day compressive strength of 9.1 MPa. The geopolymerization products of tungsten tailing-based geopolymers were N-A-S-H gels and aluminosilicate zeolite crystals, while tungsten tailings were wrapped by metakaolin-derived geopolymerization phases as aggregates with interfaces containing Si–O–Si bonding between quartz in tungsten tailings and zeolite and/or gel phase in metakaolin-derived geopolymer in the geopolymerization process. Besides, the leaching test results indicated that the immobilization efficiency of T6M4 geopolymers for Mn and Pb derived from tungsten tailings reached up to 97.28% and 99.95%, respectively. This research results provide a new idea for utilization of tungsten tailings on a large scale.
研究领域:
材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2025
·
IF 7.8
Hongyuan Liu,Xueyi Lou,Yeyao Shao,Zhichao Wang,Jiamin Xiao,Kai Cen,Dingyin Chen,Qiman Xia,Wenlong Xu,Fang Fang,Yasin Orooji,Peng Liu
To address chlorophenol wastewater pollution, immobilized mixed white rot fungi (WRF) strain microsphere was designed as a solid degradation agent. This agent uses lignin peroxidase ( LiP ), manganese peroxidase ( MnP ), and laccase ( Lac ) to degrade the wastewater. Considering the diverse physical and chemical properties of the fungal microsphere, the immobilization agent formula is optimized, and comprehensive environmental factor design response surface analysis are implemented to determine the optimal delivery conditions. Consequently, the 2,4-dichlorophenol (2,4-DCP) treatment rate and extracellular enzyme activity for a 1:1 encapsulation of T. versicolor and P. sajor-caju significantly outperform those of individual strains. The degradation efficiency reached 85.81%, with Lac activity reaching 31.2 U/mL and LiP activity reaching 29.8 U/mL. Using polyvinyl alcohol (PVA), sodium alginate (SA), and biochar as carriers, and sodium dihydrogen phosphate solution as crosslinker, with SiO 2 /zeolite as additives, the immobilized mixed fungi yielded a high-quality solid agent. This achieved a 99.33% 2,4-DCP degradation rate over 96 hours, with optimal dosage, pH, and initial 2,4-DCP concentration at 11.5 g/L, 5.5, and 40 mg/L, respectively. The degradation of 2,4-DCP by WRF selectively removes adjacent chlorine atoms to produce 4-CP, enhancing the dechlorination efficiency.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2020
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IF 4.008
Song Cao,Fei Teng,Ting Wang,Xingxing Li,Jiahui Lv,Zhonghua Cai,Yi Tao
Microalgae immobilization has been developed as a prospective method to improve the culturing and harvesting of microalgae cells in wastewater; it is also an effective method to control membrane fouling in an algal membrane system. In this study, a continuous immobilized microalgae membrane bioreactor with alginate-immobilized Chlorella vulgaris beads was developed for investigating the progress on nutrient removal and the characteristics of membrane fouling through synthetic wastewater treatment . High initial total biomass (more than 0.52 g/L) led to significant fluctuations in nutrient removal. The growth pattern of microalgae cells within the beads was explored for a better understanding of the fate of immobilized microalgae. The results showed that a moderate initial cell density of microalgae cells (7.4 × 10 5 cell/bead) in the immobilized beads achieved relatively higher specific growth rate than that of a high density, and higher nutrient removal efficiency than that of a low density. The fluorescence microscopy results of a hemisphere cut from the immobilized beads indicated that a shading layer was formed with high-density microalgae cells after a 2-day cultivation. Thus, setting a proper initial cell density of microalgae in immobilized beads was vital to achieve better performance on nutrient removal and microalgae cultivation and harvesting in the continuous operation of a reactor. Immobilization is highly effective for retarding membrane fouling through the reduction in suspended microalgal cells, mitigating the release of macromolecule algal organic matter from the immobilized beads, and regular replacement of the immobilized beads. The results and optimized directions discussed in this paper provide integrated clues and suggestions for future microalgal immobilization-related development.
研究领域:
环境生物学
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废水处理工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
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IF 13.3
Liying Yang,Xinyu Yang,Jiatai Guo,Zhengyi Yang,Yuhang Du,Qiqi Lu,Guohua Zhong
Managing invasive plant presents a significant challenge to both the environment and the economy. There is an opportunity to address this issue by utilizing invasive plants as a sustainable and cost-effective source of valuable functional materials for environmental remediation. In this study, a promising Mikania micrantha biochar (MB) was synthesized by pyrolyzing an invasive plant, which was utilized for bacterial immobilization and adsorption of contaminants. By embedding Proteus terrae ZQ02-MB within a polyvinyl alcohol-sodium alginate gel to create a biochar-modified bacterial microsphere (BBM), the superior degradation of pesticide contaminants was observed. Taking chlorothalonil (CHT) as an example, field experiments demonstrated that BBM achieved a removal efficiency of 59.8% by the third day. Additionally, BBM not only improved the richness and diversity of soil bacteria but also enhanced the microbial community structure by the seventh day. On this basis, the adsorption capacity of MB, biofilm growth promoting ability of BBM, and the CHT mineralization ability of strain ZQ02 were validated as a joint mechanism to promote efficient CHT removal. Overall, this study proposes a composite model of invasive plant-derived biochar loaded microorganisms, which has great potential in eliminating pollutant residues and paves new paths to develop strategies for reusing invasive species resources.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SOILS AND SEDIMENTS
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2021
·Vol. 21
·期 7
·2540-2550
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IF 3.308
Yang Han,Ye Zhi,Feng Fang,Xu Nan,Yang Jing,Yao Zihan,Li Zuling,Chen Jianping
Purpose Lead (Pb(II)) can be accumulated in soil and transferred to humans via the food chain. Therefore, it is essential to improve Pb(II) immobilization during soil remediation. Materials and methods Nanosized zero-valent iron (nZVI) from green tea extract (tea polyphenols, TPs) was successfully synthesized as an additive reductant. Further, the nZVI was supported on montmorillonite (Mont), resulting in a GT-nZVI@Mont composite characterized by multiple techniques, including Zeta potential, SEM, TEM, XRD, and XPS. Moreover, the transport experiments and the toxicity characteristic leaching procedure (TCLP) were conducted to identify the optimum conditions (i.e., GT-nZVI@Mont dosage, Pb(II) concentration, and pH) for the remediation. Results and discussion The experimental results showed that the enhanced transportability of GT-nZVI@Mont was about 40% more than that of GT-nZVI. Meanwhile, Pb(II) immobilization was increased at various pH values (especially under anoxic conditions). The Pb(II) immobilization capacity of GT-nZVI@Mont was 900.8 mg∙g -1 (per unit of Fe content), which was significantly higher than 530.1 mg∙g -1 per unit of Fe content in GT-nZVI. Such enhancement could be ascribed to the synergistic effects of the wrapped TP around the Fe 0 core and the Mont interlayer support, capable of protecting the Fe 0 surface from further oxidation. When GT-nZVI@Mont reacted with the Pb(II) in soil, TPs’ unwrapped coverage increased the reaction sites of Fe 0 cores for Pb(II) reduction and adsorption. Conclusions GT-nZVI@Mont with sufficient transportability provided efficient in situ remediation of Pb(II) in contaminated soil at a wide pH range.
研究领域:
环境科学
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纳米材料
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土壤学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
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2024
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IF 5.9
Siyi Liao,Jing Bai,Xue Xia,Xuanzhe Liao,Yuling Zhang,Qiulin Deng,Faqin Dong
In this work, hydroxyapatite (HAP), an environmentally friendly material was prepared with Phosphogypsum (PG) as calcium source, and uranium immobilization gel spheres were prepared by chitosan (CS) embedding, which would reduce HAP agglomeration while providing an abundance of uranium-loving groups, realizing “treating waste for waste”. The optimum pH for U(VI) removal was determined to be 4, the optimum dosage was 0.2 g/L, and q max = 350.88 mg/g through batch experiments. When C 0 = 10 mg/L, it was found that the removal rate of U(VI) by HAP-CS could be up to 99.92 %, and the residual uranium concentration was 0.008 mg/L, which meets the national emission standard. The removal rate of uranium can reach 95.92 % by applying it to high concentration uranium ore wastewater. CS was found to have good biocompatibility with HAP by SEM, FTIR, XRD, and XPS characterization. Meanwhile, we found that Ca, –OH, and P-O play a dominant role in the uranium removal process, and the main mechanism of uranium removal by Hydroxyapatite-Chitosan gel spheres (HAP-CS) is surface complexation, ion exchange, and dissolution–precipitation binding. The HAP-CS has high uranium removal efficiency, simple solid–liquid separation, and good biocompatibility, and can be used to treat actual uranium-containing wastewater.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 32
·10462-10471
·
IF 7.393
Jingjun Yang,Qingyuan Li,Congcong Ding,Lili He,Liang Xue,Wencai Cheng,Zhentao Zhang,Guilin Wei,Yi Liu,Long Huang,Yi Xie,Xirui Lu
This study is dedicated to investigating the performance of B2O3–Bi2O3–ZnO low-temperature glass-ceramics to immobilize iodine-loaded silver-coated silica-gel (AgIs). Various mass fractions of iodine (15, 20, 25, and 30 wt%) in AgIs were studied. The degree of amorphization and density increased with increasing sintering temperature for all immobilization samples, where, the sample with 20 wt% iodine in AgIs presented optimal immobilization results at 600 °C. The diffraction peak area of AgI was the lowest and the degree of amorphization was the highest (0.562) for the sample with 20 wt% iodine in silver-coated silica gel when it was sintered at 600 °C. SEM-EDS results showed that two approaches contributed to the immobilization of AgIs: the first one is that AgIs participates in the formation of the glass-ceramics phase, which makes iodine uniformly immobilized in the glass mesh; the second one is that the glass-ceramics phase physically encapsulates a small range of aggregated AgI. The results of TGA-DSC analysis showed that the samples had a high water content, which facilitated the lowering of the glass transition temperature of the samples and thus the curing of iodine. Most importantly, when the sintering temperature is below 600 °C, the evaporation of iodine is minimal to negligible. The leaching results showed that the samples were highly resistant to leaching of elemental iodine. This experiment verified the feasibility and efficiency of B2O3–Bi2O3–ZnO ternary glass-ceramics to immobilize iodine-loaded silver-coated silica-gel at low temperatures.
研究领域:
材料科学
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环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
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IF 9.038
Yi Liu,Bingsheng Li,Xiaoyan Shu,Zhentao Zhang,Guilin Wei,Yi Liu,Shunzhang Chen,Yi Xie,Xirui Lu
To better deal with the radioactive iodine generated during the development of nuclear energy, B 2 O 3 , Bi 2 O 3 , ZnO, and SiO 2 were used to sinter borosilicate glass for the immobilization of iodine. The effect of B 2 O 3 on glass formation was discussed by changing the molar ratio of B 2 O 3 in the matrix. When B 2 O 3 content is 50 mol% and sintering temperature is 600 ℃, the amorphous degree of quaternary glass is the highest. The sintered body with the highest degree of amorphous was selected to study radioactive iodine. Then, the effects of different iodine loading concentrations for sintering borosilicate glass in terms of microstructure and phase change were discussed. With the increase in iodine content on silica-gel, the degree of amorphous of the specimens presented a decreasing trend, and there are obvious SiO 2 peaks. When the content was 20 wt. %-30 wt. %, a large number of new phases were generated. When the iodine content is 20 wt. %, in addition to the enrichment of Si and O elements, the elemental distribution for B, Bi, Zn, I, and Ag was even. TEM results also showed that there was a crystalline phase in the sinter.
研究领域:
材料科学
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核能工程
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 43
·36849-36856
·
IF 3.289
Ye Yang,Ping Su,Kangle Zheng,Ting Wang,Jiayi Song,Yi Yang
This study focused on the development of a common method for the reversible and self-directed immobilization of enzymes based on a DNA-directed immobilization (DDI) technique. The successful anchoring of alkaline phosphatases to the surfaces of magnetic nanoparticles was confirmed using confocal laser scanning microscopy, thermogravimetric analysis and a vibrating sample magnetometer. The length of the DNA linker was optimized, with a base number of 24 providing maximum efficiency for the enzymolysis. Notably, the surface of alkaline phosphatase-functionalized magnetic nanoparticles could be regenerated using a mild dehybridization process of DNA. Furthermore, the resulting single-stranded probe DNA-modified magnetic nanoparticles could be reused to immobilize the alkaline phosphatase, which suggested that the DNA-functionalized surface of magnetic nanoparticles exhibited good reversibility. The biological activity of anchored alkaline phosphatases is evaluated in an enzyme inhibition assay. The results revealed that theophylline exhibited greater inhibitory activity than L-phenylalanine. The proposed protocol demonstrates a simple, mild and economic pathway for fabricating enzyme modified nanomaterial and can be applied in the high-throughput screening of inhibitors.
研究领域:
生物化学
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纳米生物学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 2
·366
·
IF 4.2
Zhuojian Lu,Rui Ge,Bin Zheng,Peng Zheng
Antibody arrays play a pivotal role in the detection and quantification of biomolecules, with their effectiveness largely dependent on efficient protein immobilization. Traditional methods often use heterobifunctional cross-linking reagents for attaching functional residues in proteins to corresponding chemical groups on the substrate surface. However, this method does not control the antibody’s anchoring point and orientation, potentially leading to reduced binding efficiency and overall performance. Another method using anti-antibodies as intermediate molecules to control the orientation can be used but it demonstrates lower efficiency. Here, we demonstrate a site-specific protein immobilization strategy utilizingOaAEP1 (asparaginyl endopeptidase) for building a nanobody array. Moreover, we used a nanobody-targeting enhanced green fluorescent protein (eGFP) as the model system to validate the protein immobilization method for building a nanobody array. Finally, by rapidly enriching eGFP, this method further highlights its potential for rapid diagnostic applications. This approach, characterized by its simplicity, high efficiency, and specificity, offers an advancement in the development of surface-modified protein arrays. It promises to enhance the sensitivity and accuracy of biomolecule detection, paving the way for broader applications in various research and diagnostic fields.Keywords:protein immobilization;enzymatic ligation;OaAEP1;nanobody
研究领域:
生物化学
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分子生物学
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诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Binbin Shao,Zhifeng Liu,Guangming Zeng,Yang Liu,Xin Yang,Chengyun Zhou,Ming Chen,Yujie Liu,Yilin Jiang,Ming Yan
In this study, the hollow mesoporous carbon spheres (HMCs) were synthesized and modified for laccase (Lac) immobilization, and the structural characteristics of HMCs materials were determined by FESEM, TEM and FTIR etc. The maximum loading of Lac on the HMCs materials could reach 835 mg/g, meanwhile, the immobilized Lac exhibited excellent thermo-stability, pH stability, storage stability and reusability. The antibiotics removal experiments indicated that the immobilized Lac possess efficient removal efficiency for both tetracycline hydrochloride (TCH) and ciprofloxacin hydrochloride (CPH) in the presence of redox mediator. The synergy of the adsorption by HMCs and the degradation by Lac could be the reasons for the high removal of antibiotics. Meanwhile, for investigating degradation mechanism, the degradation product analysis and molecular docking method had been introduced to this study. According to the degradation products, dehydroxylation and demethylation are major degradation reactions for TCH degradation, and the oxidation of the piperazinyl substituent and hydroxylation are the major degradation for CPH degradation. The docking results showed that some important residues played the key role in the degradation process. This study indicated that the immobilization of Lac on HMCs could be potentially applied in environmental remediation of antibiotics.
研究领域:
环境科学
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生物技术
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 12
·1911
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IF 4.7
Le Wang,Jianguang Liu,Yan Shen,Yanli Yin,Zifu Ni,Jun Xi,Yuansen Hu,Qipeng Yuan
The preparation of a novel composite carrier of polydopamine-modified carbon fiber/polyurethane foam (PDA-CF/PUF) was proposed to improve cell immobilization and the fermentation of xylitol, which is an important food sweetener and multifunctional food additive.Candida tropicaliswas immobilized on the composite carrier by adsorption and covalent binding. The properties and immobilization mechanism of the composite carrier and its effect on immobilized cells were investigated. It showed that the modification of PDA enhanced the loading of CF on the PUF surface and the adhesion of cells on the composite carrier surface. Also, the biocompatibility of carriers to cells was improved. In addition, the introduction of PDA increased the active groups on the surface of the carrier, enhanced the hydrophilicity, promoted the cells immobilization, and increased the xylitol yield. It was also found that expression of the related gene XYL1 in cells was significantly increased after the immobilization of the PDA-CF/PUF composite carrier during the fermentation. The PDA-CF/PUF was an immobilized carrier with the excellent biocompatibility and immobilization performance, which has great development potential in the industrial production of xylitol.
研究领域:
生物技术
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）