MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yinghua Zhu,Youduo Zhao,Jingjing Deng,Tianshu Zhou
The widespread application of neomycin (NEO) has raised pollution problem caused by the accumulation of its residues, which poses potential risk to human health and ecological safety. Therefore, the development of sensitive and reliable approaches is crucial for detecting and monitoring low levels of NEO in complex samples. In this study, an innovative spherical nucleic acid (SNA) based sensor was designed for the selective detection of NEO. The colorimetric response was attributed to hydrogen bonding, electrostatic interaction, and coordination between the SNA and NEO. Meanwhile, the fluorescence of FAM-labeled SNA was quenched by NEO due to fluorescence resonance energy transfer (FRET) effect. By integrating visual colorimetry and fluorescence analysis, the dual-responsive sensor enabled rapid, selective, and sensitive determination of NEO, with detection limits of 40.0 nM and 3.3 nM, respectively. Furthermore, the SNA exhibited good salt tolerance, maintaining stability without aggregation even in high-salt conditions. The average recoveries for detecting NEO in water samples analysis ranged from 97.6 % to 108.7 %, confirming its reliability and accuracy in practical applications. This work provides an effective strategy for NEO detection and expands the potential applications of SNA in environmental monitoring and pollution control.
研究领域:
生物化学
·
环境科学
·
分析化学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Huixiang Ouyang,Aihui Liang,Zhiliang Jiang
The stable Cu 2 O nanocubic (Cu 2 ONC) sol was prepared, based on graphene oxide (GO) catalysis of glucose-Fehling's reagent reaction, and its absorption and resonance Rayleigh scattering (RRS) spectra, transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS) were examined. Using the as-prepared Cu 2 ONC as RRS probe, and coupling with the neomycin sulfate (NEO) complex reaction , a new, simple, sensitive and selective RRS-energy transfer (RRS-ET) method was established for detection of neomycin sulfate, with a linear range of 1.4–112 μM and a detection limit of 0.4 μM. The method has been applied to the detection of neomycin sulfate in samples with satisfactory results.
研究领域:
分析化学
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生物医学检测
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产品关联
DOI
PubMed
高影响因子
ARCHIVES OF PHARMACAL RESEARCH
·
2016
·Vol. 39
·期 6
·762-770
·
IF 2.49
Wang Nan,Cui Cheng-Bin,Li Chang-Wei
A novel cyclic dipeptide, named penicimutide ( 1 ), and four known cyclic dipeptides, cyclo( l -Val- l -Pro) ( 2 ), cyclo( l -Ile- l -Pro) ( 3 ), cyclo( l -Leu- l -Pro) ( 4 ) and cyclo( l -Phe- l -Pro) ( 5 ), were isolated from a neomycin-resistant mutant of the marine-derived fungus Penicillium
purpurogenum G59. The structure of 1 , including the absolute configuration, was determined by spectroscopic and chemical methods, especially NMR and Marfey’s analysis. An unusual amino acid in 1 , 4,5-didehydro- l -leucine, was found for the first time occurring in nature. HPLC–ESI–MS analysis evidenced that 1 – 3 were produced only in the mutant strain, but 4 and 5 were produced in both the mutant and parental strains, indicating that the introduction of neomycin-resistance in the mutant activated pathways of 1 – 3 biosynthesis that were silent in the parental strain. Compound 1 selectively inhibited HeLa cells (among five tested human cancer cell lines) with an inhibition rate (IR %) of 39.4 % at 100 µg/mL, a similar inhibition intensity to that of the positive control 5-fluorouracil (IR % of 41.4 % at 100 µg/mL against HeLa cells). The present work exemplifies the effectiveness of our previous DMSO-mediated method for introducing drug-resistance in fungi to activate silent biosynthetic pathways to obtain new bioactive compounds.
研究领域:
微生物学
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药物化学
·
肿瘤学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Gang Tian,Jingyu Lin,Yu Zhou,Ziyu Huang,Xiaoping Wei,Jianping Li
A novel extended-gate field-effect transistor based molecularly imprinted polymer photoelectrochemical (EGFET-MIP-PEC) sensor was constructed for the detection of neomycin in meat samples. Bi 2 O 3 /TiO 2 NTs heterojunction materials were synthesized by anodic oxidation and electrodeposition. With neomycin as the template and 3-aminophenylboronic acid as the functional monomer, a molecularly imprinted film was prepared on the Bi 2 O 3 /TiO 2 NTs electrode by electropolymerization. The MIP/Bi 2 O 3 /TiO 2 NTs electrode was connected to an extended-gate field-effect transistor. Under the irradiation of xenon lamp simulated visible light, the holes generated on the MIP/Bi 2 O 3 /TiO 2 NTs electrodes oxidize the neomycin adsorbed by the molecularly imprinted film, generating a photovoltage, which causes the gate voltage of the extended-gate field-effect transistor to change, thereby increasing the source-drain current. The sensor showed good linearity with the source-drain current in the neomycin concentration range of 5.0 × 10 −13 –5.0 × 10 −8 mol L −1 , with a detection limit of 1.0 × 10 −13 mol L −1 (3σ/S). Owing to the amplification of EGFET on photoelectrochemical signal produced by the Bi 2 O 3 /TiO 2 NTs heterojunction with high photoelectric conversion efficiency and wider wavelength range of optical response, the sensitivity of the sensor was significantly improved. The selectivity of the sensor was remarkably enhanced due to the MIP film.
研究领域:
分析化学
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生物传感技术
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产品关联
DOI
高影响因子
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