CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Haichao Cao,Yue Chen,Daxia Zhang,Yan Jin,Peng Zhang,Beixing Li,Wei Mu,Feng Liu
In the preparation of microcapsules (MCs) by interfacial polymerization, octaphenyl polyoxyethylene (OP) is utilized as an emulsifier to control the particle size distribution of MCs. In the preparation system with polyisocyanate as the oil phase monomer, OP has a single hydroxyl (–OH) group that can react with the isocyanic group (–CNO) to prevent polymerization, and the aggregation of OP at the oil–water interface affects the polymer configuration. By the “steric hindrance” and “polymer chain end sealing” of OP, the compactness of the shells was reduced significantly to produce soft MCs. As OP-10 was added and used to increase in pyraclostrobin-loaded MCs preparation, the deformation, adhesion and release of pyraclostrobin-loaded MCs were improved. Therefore, the water scouring resistance of MCs on peanut leaves increased significantly as the MCs became soft. Unfortunately, the safety of zebrafish ( Danio rerio ) declined at the same time. By foliar spraying to control peanut leaf spot in the rainy season, MCs-2% OP-10, with the strongest resistance to rainfall, had the best effect on disease control. In conclusion, an effective strategy is to balance the foliar application efficiency and ecotoxicity of pesticide-loaded MCs by regulating the compactness and components of shells to control their deformation, adhesion and pesticide release.
研究领域:
农业科学
·
环境科学
·
材料科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Aiping Wang,Yue Sun,Zhengyi Sun,Xiao Liu,Xin Yu,Ke Li,Xianxia Zhang,Yue Xu,Wei Mu,Beixing Li
The behavior of pesticide particles or droplets might significantly influence their environmental risks. However, studies on the risk of different pesticide formulations in aqueous environments have rarely been reported. In this study, we prepared three types of pyraclostrobin formulations to evaluate their behavior in the aqueous environment and toxicological risks to zebrafish. The results showed that pyraclostrobin emulsifiable concentrate (EC) sank faster in water with increasing hydrophilicity and density of the solvent. The particles also sank faster with increasing particle size and particle density for suspension concentrate (SC) and microcapsules (MCs). Diverse behavior in water results in different temporal and spatial distributions of the active ingredient. EC-EGDA, SC-5 μm, CS-Large and EC-MO sink or float over time, therefore reducing the effective dose suspended in water. Lower toxicological risks of the pesticides were also observed by reducing the enrichment of pyraclostrobin in zebrafish. In addition to the direct toxicity of the active ingredient, the type of pesticide formulations and their specific compositions might also influence the integrated toxicity. The environmental behavior of pesticide formulations should also be considered for their systematic assessment of environmental risks to ensure the scientific application of pesticides in different scenarios.
研究领域:
环境科学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Xiaohong Zhang,Junfan Niu,Zhiyuan Zhou,Gang Tang,Guangyao Yan,Yulu Liu,Jialu Wang,Gaohua Hu,Jianhua Xiao,Weiyao Yan,Yongsong Cao
The low bioavailability of pesticides has resulted in serious resource waste, environmental pollution, and potential threats to human health. Polymeric micelles for the delivery of pesticides can obviously reduce the drift, and photolysis as well as the risks of poisoning during the application of pesticides. Herein, an amphiphilic polymer, octaneacylhydrazone propylcellulose pyruvate (OPP), was prepared by conjugating octanehydrazide with propylcellulose pyruvate and used as a micellar carrier to improve the utilization efficiency of pyraclostrobin (PYR). The results showed that OPP exhibited a lower critical micelle concentration (9.99 mg/L) than classical surfactant Tween. The prepared PYR-loaded OPP micelles (POM) exhibited excellent pH and cellulase dual responsive release properties with a pesticide loading rate of 8.94%, which could efficiently prevent PYR from photolysis to prolong the effective duration. The fungicidal activities of POM against Sclerotinia sclerotiorum and Botrytis cinerea were better than that of PYR in vitro and in vivo . Furthermore, the acute toxicity of POM to zebrafish was decreased more than eleven-fold compared with that of PYR technical. Therefore, the prepared POM with excellent fungicidal activities may be a potential alternative to conventional formulations of PYR, and OPP would be used as a promising polymeric carrier for the highly efficient delivery of pesticides.
研究领域:
材料科学
·
农业科学
·
环境科学
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产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2026
·
IF 4.8
Yunxiang Qi,Zhenghua Zhang,Xuan Wang,Haitao Long,Lumei Pu,Weibing Xu
Smart nanopesticides demonstrates excellent potential in the prevention of tomato gray mold. Herein, temperature, pH and GSH triple responsive of pyraclostrobin nanopesticide (CDC-F127-P) is fabricated base on Pluronic F127 and cinnamaldehyde-derived liposome. The maximum fungicide loading of Pyr reaches 52.9%. The particle size and zeta potential are 143 nm and + 29 mV. The release of Pyr increases from 38% to 61% after 48 h as the temperature rises from 5 °C to 35 °C. The value increases from 62% to 87% when the concentration of oxalic acid rises from 0 to 20 mM, which increases from 10% to 37% as the concentration of GSH rises from 0 to 20 mM. The EC₅₀ of CDC-F127-P is 1.96 μg/mL. The inhibition rates of CDC-F127-P for mycelial growth and spore germination are 69.3% and 73.0% at 10 μg/mL. The nucleic acid and protein leakage reaches 124.8 μg/mL and 113.5 μg/mL. A spore mortality rate of 66.4% is arrived. Mitochondrial membrane potential reduces by 96% after treatment with 2 μg/mL. The CDC-F127-P shows a hemolysis rate of 1.8% and a tomato seed germination rate of 60% at 10 μg/mL. The contact angle on the tomato leaf is 74.0 o . A solution retention capacity of 27.5 mg/cm 2 is retained after simulated rainfall. The disease inhibition rates of 64.9% and 71.0% in detached-leaf and potted-plant assays after treatment with 10 μg/mL CDC-F127-P. The triple-responsive CDC-F127-P nanopesticide offers a highly efficient and safe strategy for gray mold control and presenting a novel approach for developing green nano-pesticides.
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Tianyang You,Zhiying Zhang,Jinyu Ma,Yin Jia,Yuan Ding,Xiude Hua
Pyraclostrobin is widely employed for controlling fungal pathogens. Given its potential adverse effects on aquatic organisms , beneficial insects, and mammals, there is an urgent need to develop on-site detection methods for monitoring pyraclostrobin residues. In this study, monoclonal antibodies (mAbs) targeting pyraclostrobin were developed, and a reliable structure of the mAb 4B4C3 was predicted using AlphaFold 3. The structure showed high predicted local distance difference test (pLDDT) values for both the complementarity-determining regions (CDRs) and framework regions (FRs). Molecular docking was employed to investigate its binding pattern with pyraclostrobin. Subsequently, a highly sensitive lateral flow immunoassay (LFIA) based on gold nanoparticles (AuNPs) for detecting pyraclostrobin was established. The AuNP-LFIA method exhibited a visual limit of detection (vLOD) of 1.25 ng/mL and a completely inhibitory concentration of 5 ng/mL, surpassing the sensitivity of previously described methods. The accuracy of the established method was confirmed through spiked-recovery tests, exhibiting a sensitivity of 30 ng/g in wheat, apple, and soil samples, and 60 ng/g in Chinese cabbage samples. The reliability of the method was validated using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). This study presents a sensitive and cost-effective approach for rapidly detecting pyraclostrobin residues, demonstrating the significant potential of AlphaFold 3 in antibody engineering and small molecule on-site detection.
研究领域:
农业化学
·
分析化学
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环境科学
·
分子生物学
·
纳米技术
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Xueping Huang,Jian Luo,Haichao Cao,Aiping Wang,Fengyan Zhou,Feng Liu,Beixing Li,Wei Mu,Yong Zhang
Developing microcapsules (MCs) delivery systems can effectively mitigate toxicological risk of highly active/toxic pesticides; whereas the controlled release functions also limiting their practical effectiveness. Therefore, designing a precise regulating strategy to balance the toxicity and bioactivity of MCs is urgently needed. Here, we prepared a series of pyraclostrobin-loaded MCs with different wall materials, particle sizes, core density and shell compactness using interfacial polymerization. The results showed that the MCs released more slowly in water with increasing particle sizes and capsule compactness, and they sunk more quickly with the increasing particle sizes and core density. Additionally, MCs with slower release speed was always accompanied with lower acute toxicity levels to zebrafish. When the release dynamics slowed down to the threshold dose on demand for disease control, facilitating settlement of MCs can further reduce toxicity within spatial and temporal dimensions. The poor accumulation of MCs with larger particle sizes or dense shell in gills was closely related to their efficient detoxification. Importantly, seven of the MCs samples possessed superior selectivity between bio-performance in controlling rice blast and toxicological hazard to fish compared to commercial formulations. The results provide a comprehensive guidance for developing an efficient and safe pesticide delivery system.
研究领域:
农药科学
·
毒理学
·
农业化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2026
·Vol. 49
·期 3
·e70385
·
IF 3.6
Chunpeng Feng,Yufeng Guo,Yunlong Sheng,Meichen Liu,Jiale Liu,Yue Deng,Shuang Feng,Yalin Xi,Meiyun Shi,Lei Yin
Pyraclostrobin, a widely applied methoxyacrylate fungicide, poses considerable risks to aquatic ecosystems due to its environmental persistence and bioaccumulation potential. Understanding its tissue-specific residue profile in aquatic organisms such as zebrafish is essential for elucidating ecotoxicological mechanisms and evaluating environmental safety. In this study, a novel, rapid, and sensitive ultra‑high‑performance liquid chromatography–triple‑stage mass spectrometry (UHPLC‑MS 3 ) method was developed and fully validated for the quantification of pyraclostrobin in zebrafish liver tissue. The assay utilized an MS 3 transition ( m/z 388.0 → 194.0 → 164.0), which significantly enhanced selectivity and sensitivity. The method achieved fast separation (3 min per sample) and exhibited excellent linearity from 1 to 300 ng/mL ( R 2 ≥ 0.998). Precision (intra‑ and inter‑day coefficient of variation <10.2%), recovery (94.4%–95.4%), and matrix effects (99.2%–108.9%) all met rigorous bioanalytical criteria. This work represents the first application of UHPLC‑MS 3 for strobilurin analysis in aquatic tissues, providing a reliable high‑throughput platform for residue monitoring and mechanistic toxicology research.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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