ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2020
·Vol. 412
·期 19
·4555-4563
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IF 3.637
Huang Yukun,Wang Chong,Huo Qiuyu,Song Yaning,Du Gang,Wang Lijun,Yang Xiao,Chen Xianggui
A novel sensitive, competitive, and time-resolved luminescence sensor for the detection of ofloxacin (OFL) was developed in this study. The sensor used OFL-specific aptamer as a recognition molecule and rolling circle amplification (RCA) as a signal amplification tool. In this way, the time-resolved luminescence can not only avoid background noise from sample, but also provide robust luminescence for detection. Besides, the separation and enrichment of target veterinary drug can be conducted assisted by magnetic treatment. Under optimal conditions, the logarithmic correlation between the concentration of OFL and the luminescence intensity was found to be linear in the range of 5 × 10 −11 –5 × 10 −8 mol L −1 ( R 2 = 0.9988), with a detection limit (LOD) of 32.1 pmol L −1 . Furthermore, this method was applied to the determination of OFL in chicken and pork samples, exhibiting good recovery (72.5–100%) and repeatability (RSD < 10.0%). These results confirm that this novel established method has good application potential for the detection of OFL in food samples.
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高影响因子
BMC Complementary Medicine and Therapies
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2020
·Vol. 20
·期 1
·1-9
·
IF 0
Li Jiashun,Jie Xiang,Liang Xiaoli,Chen Ziyu,Xie Peifang,Pan Xiping,Zhou Beixian,Li Jing
Background Human respiratory system infected with influenza A virus (IAV) elicited a robust pro-inflammatory response that resulted in severe illness and even death. Currently, limited immunomodulator is available to counteract IAV-associated pneumonia in the clinic. Sinensetin, a polymethoxylated flavone with five methoxy groups, has been found to possess anti-agiogenesis, anti-inflammatory and anti-diabetic activities. However, the effects of sinensetin on IAV-triggered pro-inflammatory response remain unclear. In the present study, the anti-inflammatory effects and corresponding possible mechanism of sinensetin in IAV-infected A549 cells were subjected to investigations. Methods The cytotoxic effects of sinensetin towards A549 cells was detected by MTT and LDH assays. The antiviral activity of sinensetin against influenza A virus was assayed in A549 cells with an engineered replication-competent influenza A virus carrying Gaussia luciferase reporter gene infection. The effect of sinensetin on influenza A virus-triggered inflammatory reaction was determined by qRT-PCR, Luminex assays, ELISA and Western blot. Results Our results showed that sinensetin did not exhibit antiviral activity against A/PR/8/34 (H1N1). Meanwhile, sinensetin treatment significantly decreased IAV-induced expression of pro-inflammatory mediators at mRNA and protein levels, including IL-6, TNF-α, IP-10, IL-8 and MCP-1. Additionally, levels of cyclooxygenase (COX)-2 and the downstream product prostaglandin E 2 (PGE 2 ) up-regulated by IAV infection were dramatically suppressed by sinensetin. The mechanistic investigation revealed that sinensetin treatment suppressed the NF-κB transcriptional activity using the NF-κB reporter stable HEK293 cell line stimulated with TNF-α (20 ng/mL) or influenza H1N1 virus. Furthermore, sinensetin abrogated influenza H1N1 virus-induced activation of NF-κB, ERK1/2 MAPK and p38 MAPK signalings. Conclusion Collectively, our results indicated that sinensetin has potential capacity to attenuate IAV-triggered pro-inflammatory response via inactivation of NF-κB, ERK1/2 MAPK and p38 MAPK signalings, which implied that sinensetin may be a promising candidate drug for influenza H1N1 virus infection therapeutics.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2020
·Vol. 31
·期 9
·6866-6874
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IF 2.22
Zhao Yongke,Zhou Zheng,Chen Guangxin,Li Qifang
The metal–organic frameworks have gained high reputation due to their porous structures, high stabilities, ultralow densities, devisable organic ligands, and large specific surface area in recent years. In this work, ZIF-8@silane-modified sepiolite hybrids with a core–shell structure were synthesized successfully via an in situ grown method. The hybrids were then introduced into epoxy to prepare composites. Results showed that the epoxy composites exhibited improved dielectric and thermal properties when the amount of the fillers was only 0.6 wt%. The dielectric constant was decreased by 0.66, the thermal degradation temperature was increased by 46.2 °C, and the glass transition temperature was increased by 8.5 °C.
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JOURNAL OF MATERIALS SCIENCE
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2020
·Vol. 55
·期 19
·8403-8419
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IF 3.553
Zhang Yuhui,Lin Ligang,Wang Qi,Qiang Rongrong,Gao Yixin,Ma Sisi,Cheng Qi,Zhang Yuzhong
A new strategy was proposed that simultaneously improves the pervaporation (PV) performance and solvent resistance via the construction of a movable crosslinked structure in ethyl cellulose/polyvinylidene fluoride (EC/PVDF) composite membrane for the purpose of benzene/cyclohexane separation. Based on the molecular design of the macrocyclic compound cyclodextrin, polyrotaxane crosslinking agent (PR=) with a special sliding/rotating property and cavity structure was successfully synthesized. Furthermore, for convenient comparative study, cyclodextrin crosslinking agent (CD=) with cavity structure bereft of a sliding/rotating property and pentaerythritol triacrylate (PETA) without sliding/rotating property and cavity structure were prepared. The composite membranes were fabricated with crosslinking modified EC as active layer, together with PVDF support layer. The molecular structure and microstructure were confirmed. The pervaporation and swelling performance of the fabricated membranes for benzene/cyclohexane were investigated. PR= modified membranes showed good solvent resistance and exhibited a flux of 4332 g·m −2 ·h −1 and separation factor of 6.26, which was higher than CD= and PETA modified membranes. The results were attributed to the facilitated transport behavior, which was markedly related with the sliding/rotating property and cavity structure of polyrotaxane (PR). The related research provides interesting suggestions for PV membrane design for the separation of organic mixtures.
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CATALYSIS LETTERS
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2020
·Vol. 150
·期 9
·2703-2708
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IF 2.482
Zhao Zhaohui,Wang Yanhua
Abstract Thermoregulated phase-transfer Pd nanocatalyst was firstly employed in the selective hydrogenation of 1,5-cyclooctadiene (1,5-COD) at atmospheric hydrogen pressure. Under the optimal conditions, > 99% conversion and 95% selectivity to cyclooctene (COE) were obtained. The Pd nanocatalyst can be easily separated from products and directly reused. The turnover frequency (TOF) of 1411 h −1 is the highest value ever reported among Pd nanocatalysts at atmospheric hydrogen pressure. Graphic Abstract
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2020
·Vol. 104
·期 9
·3947-3957
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IF 3.53
Qu Yuejun,Yang Yue,Du Renjie,Zhao Min
A sensing methodology that combines Au, tobacco mosaic virus (TMV), and folic acid for selective, sensitive, and colorimetric detection of tumor cells based on the peroxidase-like activity was reported in this study. Gold nanowires with a high aspect ratio were synthesized using TMV as a template. Au@TMV nanowire (AT) complex was obtained with diameter of 4 nm and length between 200 and 300 nm. In addition, since TMV was biocompatible and had many amino and carboxyl groups on its surface, AT was conjugated by folate to form a folic acid (FA)–conjugated AT composite (ATF) and tested by FTIR measurements. Furthermore, the peroxidase-like properties were studied and the optimal conditions for mimic enzyme activity were optimized. Finally, HeLa and other tumor cells expressed excessive receptors of folate on the surface, which can specifically bind to folic acid. As the specific binding of ATF with HeLa cells, the peroxidase properties of ATF were used for detection of cancer cells (Scheme 1). The cancer cells were detected not only qualitatively but also quantitatively. In this study, as low as 2000 cancer cells/mL could be detected using the current method.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2020
·Vol. 27
·期 15
·17986-17996
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IF 3.056
Wang Yanhua,Du Yingchao,Yan Jingmin,Wang Yongliang,Yu Bo,Zhang Xiaomeng,Ye Shufeng
Compared with As(V), As(III) is a tricky issue worldwide for its higher toxicity and more difficult to remove in aqueous solution. In present study, a novel CeO x /MnO y nanoparticles anchored layered structural TiO 2 pillared montmorillonite (TiO 2 -Mt-Ce-Mn) was fabricated and applied as an efficient absorbent for As(III) removal. Under the condition of the initial As(III) concentration = 20 mg/L and adsorbent dose = 0.4 g/L, TiO 2 -Mt-Ce-Mn with a high specific surface area (148.099 m 2 /g) has an outstanding adsorption capacity (46.58 mg/g) for As(III) at pH 4.2, and the effect of oxy-anions on adsorption efficiency is slight except for H 2 PO 4 − . Interestingly, the layered structure provides sufficient attachment space for CeO x /MnO y nanoparticles, while CeO x /MnO y nanoparticles in turn endows TiO 2 -Mt a high redox potential, which further facilitates the oxidation of As(III), and this significantly reduces the toxicity of wastewater. The adsorption mechanism includes the oxidation of As(III) to As(V) by both CeO x /MnO y nanoparticles and TiO 2 and effective adsorption of the residual As(III) and the formed As(V) subsequently. In addition, the adsorption efficiency of TiO 2 -Mt-Ce-Mn can still maintain 79.6% after five cycles through a facile regeneration method. Thus, the nanocomposite with low-cost synthesis process, high adsorption capacity, and regenerability is a promising candidate for As(III) treatment of wastewater.
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JOURNAL OF MATERIALS SCIENCE
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2020
·Vol. 55
·期 17
·7321-7336
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IF 3.553
Xue Ying,Li Chunmei,Tan Jiaojun,Su Zhengzhou,Yang Yumin,Zhang Guoxian,Zhang Qiuyu
A novel microcapsule-type thermal latent curing agent with a double-shell structure was successfully fabricated. 1-Benzyl-2-methylimidazole (1B2MZ) was encapsulated by poly(methyl methacrylate- n -butyl acrylate) (PMMA/BA) using the solvent evaporation method, which served as the primary microcapsules (PMCs) to be deposited by polydopamine (PDA). Various glass transition temperatures ( T g , from 116.7 to 57.2 °C) and thermal properties of shell material PMMA/BA were obtained by tuning the mass ratio of BA to MMA monomer. PMMA/BA with 20 wt% of BA ratio was selected as the shell of PMCs for its appropriate T g (92.33 °C) and relatively good thermal resistance. (Decomposition temperature was 222.83 °C.) The obtained PMCs and the double-shell microcapsules (DMCs) had good spherical shape, and the chemical structure of DMCs was confirmed by Fourier transform infrared spectra. Thermogravimetric analysis indicated that the core contents of PMCs and DMCs were 11.95 and 11.37%, respectively. After being coated by PDA, the solvent resistances of microcapsules in weak-polar (toluene) and high-polar (dichloromethane, DCM and acetone) solvents were significantly improved. When storing at room temperature for more than 4 months, the mixture of DMCs and the diluted epoxy could be cured at 120 °C for 1 h.
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Nature Communications
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2020
·Vol. 11
·期 1
·1-11
·
IF 12.121
Wang Yang,Wen Xinrong,Jia Yanmin,Huang Ming,Wang Feifei,Zhang Xuehui,Bai Yunyang,Yuan Guoliang,Wang Yaojin
The increasing demand for a whiter smile has resulted in an increased popularity for tooth whitening procedures. The most classic hydrogen peroxide-based whitening agents are effective, but can lead to enamel demineralization, gingival irritation, or cytotoxicity. Furthermore, these techniques are excessively time-consuming. Here, we report a nondestructive, harmless and convenient tooth whitening strategy based on a piezo-catalysis effect realized by replacement of abrasives traditionally used in toothpaste with piezoelectric particles. Degradation of organic dyes via piezo-catalysis of BaTiO 3 (BTO) nanoparticles was performed under ultrasonic vibration to simulate daily tooth brushing. Teeth stained with black tea, blueberry juice, wine or a combination thereof can be notably whitened by the poled BTO turbid liquid after vibration for 3 h. A similar treatment using unpoled or cubic BTO show negligible tooth whitening effect. Furthermore, the BTO nanoparticle-based piezo-catalysis tooth whitening procedure exhibits remarkably less damage to both enamel and biological cells.
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Nano Research
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2020
·Vol. 13
·期 3
·638-645
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IF 8.183
Lei Wenjuan,Li Menggang,He Lin,Meng Xun,Mu Zijie,Yu Yongsheng,Ross Frances M.,Yang Weiwei
The morphology and size of Pt-based bimetallic alloys are known to determine their electrocatalytic performance in reactions relevant to fuel cells. Here, we report a general approach for preparing Pt-M (M = Fe, Co and Ni) bimetallic nano-branched structure (NBs) by a simple high temperature solution-phase synthesis. As-prepared Pt-M NBs show a polycrystalline structure and are rich in steps and kinks on the surface, which promote them favorable bifunctional catalytic properties in acidic electrolytes, specifically in terms of the oxygen reduction reaction (ORR) and methanol oxidation reaction (MOR). Specially, Pt-Co NBs/C catalyst shows 6.1 and 5.3 times higher in specific activity (SA) and mass activity (MA) for ORR than state-of-the-art commercial Pt/C catalysts, respectively. Moreover, it exhibits a loss of 4.0% in SA and 14.4% in MA after 10,000 cycles of accelerated durability tests (ADTs) compared with the initial activities. In addition, we also confirmed the superior MOR activity of Pt-Co NBs/C catalyst in acidic electrolytes. For Pt-M NBs with other alloying metals, the ORR and MOR activities are both higher than commercial catalysts and are in the sequence of Pt-Co/C > Pt-Fe/C > Pt-Ni/C > commercial Pt/C (or PtRu/C). The improved activities and durability can benefit from the morphological and compositional effects. This synthesis approach may be applied to develop bifunctional catalysts with enhanced ORR and MOR properties for future fuel cells designs.
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JOURNAL OF FLUORESCENCE
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2020
·Vol. 30
·期 3
·463-470
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IF 2.093
Chen Hongxia,Peng Junxiang,Yu Long,Chen Hui,Sun Mingtai,Sun Zhenli,Ni Runyan,Alamry Khalid A.,Marwani Hadi M.,Wang Suhua
Herein, we report an interesting finding about the new application of oxytetracycline (OTC), as a fluorescent probe for the detection of calcium ion (Ca 2+ ), which proved that it can offer an expeditious, highly sensitive, and selective detection method for Ca 2+ . Upon the addition of Ca 2+ , the fluorescence of OTC could be significantly enhanced with rapid response and high sensitivity, and achieved a good limit of detection as low as 125 nM in aqueous solution. The complex formed via Ca 2+ coordinating to the hydroxyl group of OTC contributes to the fluorescence enhancement, which has been proved by several characterization methods including UV-vis analysis, binding constant determination, and fluorescence titration. The method avoided complexity for EDTA measurement of Ca 2+ in running water as proposed previously. Taking advantage of good availability, stability and operability, the OTC was further successfully applied to the detection of Ca 2+ in a real environment.
Graphical Abstract
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BMC Complementary Medicine and Therapies
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2020
·Vol. 20
·期 1
·1-17
·
IF 0
Wang Le,Tan Nana,Wang Huan,Hu Jingbo,Diwu Wenbo,Wang Xiaoling
Background Flavonoids from plant medicines are supposed to be viable alternatives for the treatment of type 2 diabetes (T2D) as less toxicity and side effects. Radix scutellariae ( RS ) is a widely used traditional medicine in Asia. It has shown great potential in the research of T2D. However, the pharmacological actions remain obscured due to the complex chemical nature of plant medicines. Methods In the present study, a systematic method combining ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology was developed to screen α -glucosidase inhibitors from flavonoids of RS , and explore the underlying mechanism for the treatment of T2D. Results The n -butanol part of ethanol extract from RS showed a strong α -glucosidase inhibition activity (90.55%, IC 50 0.551 mg/mL) against positive control acarbose (90.59%, IC 50 1.079 mg/mL). A total of 32 kinds of flavonoids were identified from the extract, and their ESI-MS/MS behaviors were elucidated. Thirteen compounds were screened as α -glucosidase inhibitors, including viscidulin III, 2′,3,5,6′,7-pentahydroxyflavanone, and so on. A compound-target-pathway (CTP) network was constructed by integrating these α -glucosidase inhibitors, target proteins, and related pathways. This network exhibited an uneven distribution and approximate scale-free property. Chrysin ( k = 87), 5,8,2′-trihydroxy-7-methoxyflavone ( k = 21) and wogonin ( k = 20) were selected as the main active constituents with much higher degree values. A protein-protein interaction (PPI) weighted network was built for target proteins of these α -glucosidase inhibitors and drug targets of T2D. PPARG ( C d = 0.165, C b = 0.232, C c = 0.401), ACACB ( C d = 0.155, C b = 0.184, C c = 0.318), NFKB1 ( C d = 0.233, C b = 0.161, C c = 0.431), and PGH2 ( C d = 0.194, C b = 0.157, C c = 0.427) exhibited as key targets with the highest scores of centrality indices. Furthermore, a core subnetwork was extracted from the CTP and PPI weighted network. Type II diabetes mellitus (hsa04930) and PPAR signaling pathway (hsa03320) were confirmed as the critical pathways. Conclusions These results improved current understanding of natural flavonoids on the treatment of T2D. The combination of ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology provides a novel strategy for the research of plant medicines and complex diseases.
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