ACS Nano
·
2024
·
IF 15.8
Wenying Li,Dong Chen,Yanmei Zhu,Qiange Ye,Yang Hua,Ping Jiang,Ying Xiang,Yuejie Xu,Yinya Pan,Hua Yang,Yichun Ma,Hang Xu,Cheng Zhao,Chang Zheng,Changrong Chen,Yun Zhu,Guifang Xu
Current therapies primarily targeting inflammation often fail to address the root relationship between intestinal mucosal integrity and the resulting dysregulated cell death and ensuing inflammation in ulcerative colitis (UC). First, UC tissues from human and mice models in this article both emphasize the crucial role of Gasdermin E (GSDME)-mediated pyroptosis in intestinal epithelial cells (IECs) as it contributes to colitis by releasing proinflammatory cytokines, thereby compromising the intestinal barrier. Then, 4-octyl-itaconate (4-OI), exhibiting potential for anti-inflammatory activity in inhibiting pyroptosis, was encapsulated by butyrate-modified liposome (4-OI/BLipo) to target delivery for IECs. In brief, 4-OI/BLipo exhibited preferential accumulation in inflamed colonic epithelium, attributed to over 95% of butyrate being produced and absorbed in the colon. As expected, epithelium barriers were restored significantly by alleviating GSDME-mediated pyroptosis in colitis. Accordingly, the permeability of IECs was restored, and the resulting inflammation, mucosal epithelium, and balance of gut flora were reprogrammed, which offers a hopeful approach to the effective management of UC.
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JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Liling Wang,Yanbin Wang,Yuchuan Qin,Liang He
U.S. pecan nuts are rich in oil and phenolic compounds with important biological functions. At present, the reports about simultaneous extraction of oil and phenolic compounds are limited, and the traditional procedure is complicated and the yield is low. In this study, a new emulsion formed by hydrophobic deep eutectic solvent (DES) and water was used as extractant for synchronous extraction of oil and phenolic components from U.S. pecans, and subsequently the two were recovered from the upper and lower phases based on the incompatibility of DES and water, respectively. The oil and phenolic compounds obtained by the optimization of extraction conditions were 615.29 mg/g and 82.11 mg/g, respectively, which were higher than those obtained by conventional methods. At the same time, the extraction mechanism is discussed through the extraction kinetics and thermodynamics. The extraction process conformed to the Fick’s second law, and it was an endothermic process. After directional recovery by macroporous resin and back-extraction, the effectiveness of the method was proved by the analysis of fatty acid composition of oil, spectral characterization of phenolic compounds and antioxidant activities.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Peng Zhao,Xuhui Xia,Jia Zheng,Zirui Yuan,Yiyao Luo,Huibo Luo,Yi Ma,Danqun Huo,Changjun Hou
The correct classification of strong-flavored crude baijiu affects its quality and overall standard and is crucial for the intelligent development of the baijiu industry. In this work, we developed a novel optical sensing array using lanthanum-doped carbon dots (La-CDs). Using La-CDs with three metal complex dyes—chromium black T, alizarin red, and dimethylphenol orange—we were able to detect organic acids and tannic acid (TA) in crude baijiu in a way that was both colorimetric and fluorescent for the first time. Based on the indicator displacement (IDA) principle, organic acids competitively replace the dyes' binding sites on La 3+ , causing the dye colors to change to varying degrees. TA quenches the fluorescence of quantum dots through an internal filtering effect. We analyzed the data using pattern recognition algorithms such as HCA, PCA, and LDA, successfully classifying and identifying 16 types of strong-flavored crude baijiu, which included 10 types of carboxylic acids and various grades. In blind tests of 32 crude baijiu samples, the colorimetric method achieved a 94 % accuracy rate, while the fluorescence method achieved 100 %. The sensor demonstrates significant advantages in response speed.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2024
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IF 4.8
Zihao He,Siyuan Deng,Zhijing Wu,Zheng Cui,Hao Mei,Jinglin Wang,Kaiping Wang,Yu Zhang
Ethnopharmacological relevance Dysfunction of the intestinal barrier was an important trigger for alcoholic liver damage and alcohol had brought about intestinal damage before causing liver damage. The root of Angelica sinensis (Oliv.) Diels, crucial traditional medicinal material, was widely utilized for its blood-invigorating, intestinal-lubricating and gynecological benefits. Angelica sinensis polysaccharide (ASP) was an essential natural active ingredient of Angelica sinensis and exhibited considerable potential for gastrointestinal protection. Nevertheless, the systematic research of ASP on the gastrointestinal tract remained insufficient. Aim of this study To systematically explore the protective effect and underlying mechanisms of ASP against alcohol-induced gastrointestinal injury, including the stomach, ileum and colon. Materials and methods The AFLD mice model was established via the intragastric administration of alcohol twice a day for one week. The protective effect of ASP on the representative segments of the gastrointestinal tract (stomach, ileum and colon) was subsequently studied after confirming its hepatoprotective activity. The impact of ASP on gastrointestinal alcohol metabolism was examined to explain its antioxidant and antiapoptotic activities. Furthermore, the effect of ASP on short-chain fatty acids (SCFA) in the colon and colonic contents was investigated to further enhance the understanding of the underlying mechanisms. Results ASP could reduce oxidative stress and apoptosis in the gastrointestinal tract via regulating CYP2E1-mediated alcohol metabolism. Additionally, ASP could significantly increase the levels of FFAR2, FFAR3 and HCAR2 in colon, thereby promoting the utilization of SCFA. Conclusion ASP was proven for the first time to improve gastrointestinal damage caused by alcohol, indicating its enormous potential as a candidate medicine for the treatment of alcohol related gastrointestinal injury.
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Analytical Methods
·
2024
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IF 2.7
Hongxu Zhou,Qing Jiang,Xin He,Xian Fu,Jun-Yan Liu
Fatty acids (FAs) are essential molecules in all organisms and are involved in various physiological and pathophysiological processes. Pentafluorobenzyl bromide (PFBBr) is commonly used for FAs derivatization for gas chromatography-mass spectrometry (GC–MS) quantification by chemical ionization (CI). While CI is the conventional ionization mode for PFBBr derivatization, electron ionization (EI) source has also demonstrated efficacy in achieving satisfactory analytical performance for the analysis of PFB esters. In this study, we present a novel approach utilizing PFBBr-derivatization on a GC-EI-MS platform to quantitatively analyze a comprehensive range of 44 fatty acids (FAs) spanning from C2 to C24. The method's sensitivity, precision, accuracy, linearity, recovery, and matrix effect were rigorously validated against predetermined acceptance criteria. In comparison to the conventional CI ionization mode, the utilization of PFBBr-derivatization in GC-EI-MS exhibits a wider range of applications and achieves comparable sensitivity levels as the conventional CI platform. By using this method, we successfully quantified the 44 FAs in plasma and feces samples from the mice with deoxynivalenol (DON)-induced kidney injury. Among these, the levels of most FA species were increased in the DON-exposure group compared with the control group. The orthogonal partial least squares discriminant analysis (OPLS-DA) of all the tested FAs showed a visual separation of the two groups, indicating DON exposure resulted in a disturbance of FA profile in mice. These results indicate that the established method by integration of GC-MS with PFBBr derivatization is an efficient approach to quantify the comprehensive FA profile, which includes short-, medium- and long-chain FAs. In addition, our study provides new insights into the mechanism underlying DON exposure-induced kidney injury.
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Food Bioscience
·
2024
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IF 4.8
Min Zhuang,Gaoheng Li,Sheng Ke,Anqi Wang,Xuanyu Wang,Zhongkai Zhou
Gut microbiota is influenced by the dietary composition and plays important roles in the pathogenesis of metabolic disorders. Thus, wheat bran extract was prepared by either water extraction or enzymatic pre-treatment extraction (referred as AEWB and EHWB, respectively), and then their corresponding impact on short-chain fatty acids generation and gut microbiota was investigated via both in vitro fermentation model and in vivo diabetic animal model. The results of in vitro model indicated that EHWB enhanced the abundance of Megamonas and Faecalibacterium , as well as the amino acids metabolism, which was consistent with the increased butyrate and amino acids metabolites (e.g. iso-butyrate and valerate). The diabetic model analysis suggested that the rats also had an increased amino acid metabolism due to the decreased pathways of amino acid biosynthesis and increased pathways of amino acid degradation following the EHWB intervention. Importantly, the current study revealed that the EHWB intervention led to a greater level of butyrate in the diabetic animal model compared to other groups, demonstrating its prebiotic function, which was supported by the positive correlation between butyrate level and abundance of Bifidobacterium and Lactobacillus . The above results indicated that the main components in water-extractable substrates played key roles in improving the gut microbiota profile and the key metabolite in terms of butyrate. This study might highlight a new ingredient for food industry with physiological functionality.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2017
·Vol. 101
·期 14
·5689-5697
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IF 3.42
Zhou Pengfei,Lan Dongming,Popowicz Grzegorz Maria,Wang Xuping,Yang Bo,Wang Yonghua
Green technologies are attracting increasing attention in industrial chemistry where enzymatic reactions can replace dangerous and environmentally unfriendly chemical processes. In situ enzymatic synthesis of peroxycarboxylic acid is an attractive alternative for several industrial applications although concentrated H 2 O 2 can denature the biocatalyst, limiting its usefulness. Herein, we report the structure-guided engineering of the Pyrobaculum calidifontis esterase (PestE) substrate binding site to increase its stability and perhydrolysis activity. The L89R/L40A PestE mutant showed better tolerance toward concentrated H 2 O 2 compared with wild-type PestE , and retained over 72% of its initial activity after 24-h incubation with 2 M H 2 O 2 . Surprisingly, the half-life ( t
1/2 , 80 °C) of PestE increased from 28 to 54 h. The k
cat / K
m values of the mutant increased 21- and 3.4-fold toward pentanoic acid and H 2 O 2, respectively. This work shows how protein engineering can be used to enhance the H 2 O 2 resistance and catalytic efficiency of an enzyme.
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CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Xiaoxue Lu,Rongrong Ma,Jinling Zhan,Xiaohua Pan,Chang Liu,Wangyang Shen,Shuang Zhang,Weibiao Zhou,Yaoqi Tian
Long-term intake of thermally processed starch-based foods may impact glucose homeostasis, but the consistency of the effects of various thermal treatments and the reasons are not clear. In this study, thermal treatments, especially boiling, damaged the crystal structure and inter-molecular hydrogen bonds of starch-based blends, thus decreasing the structural order and stability. These thermally treated starch-based blends increased the appetite of mice, promoted food digestion, and enhanced postprandial glucose response. Normal C57BL/6J mice were treated with boiled, baked, and fried starch-based diets for ten weeks. Compared to the baked and fried starch-based diets, the boiled starch-based diet significantly ( p < 0.05) elevated random blood glucose levels and disrupted insulin homeostasis, primarily due to the remarkable decrease in gut microbial diversity. Both baked and fried starch-based diets resulted in relatively high intestinal epithelial permeability (plasma lipopolysaccharide increased by 28.67 % and 21.85 %, respectively). They adversely affected islet β-cell function and evoked glucose metabolism disorder. Overall, results demonstrate a clear connection among the thermal processing of starch-based diets, disruption of intestinal homeostasis, and adverse glucose metabolism. This study lays a theoretical foundation for the formulation of food processing strategies to mitigate the adverse effects of thermally treated food on glucose homeostasis.
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iMeta
·
2025
·
IF 33.2
Yihang Zhao,Mengzhen Jia,Chen Ding,Bingkun Bao,Hangqi Li,Jiabin Ma,Weixuan Dong,Rui Gao,Xuhui Chen,Jiao Chen,Xiaoshuang Dai,Yuanqiang Zou,Jun Hu,Lin Shi,Xuebo Liu,Zhigang Liu
Time-restricted feeding (TRF) holds promise for alleviating cognitive decline in aging, albeit the precise mechanism via the gut-brain axis remains elusive. In a clinical trial, we observed, for the first time, that a 4-month TRF ameliorated cognitive impairments among Alzheimer's disease (AD) patients. Experiments in 5xFAD mice corroborated the gut microbiota-dependent effect of TRF on mitigating cognitive dysfunction, amyloid-beta deposition, and neuroinflammation. Multi-omics integration linked Bifidobacterium pseudolongum ( B. pseudolongum ) and propionic acid (PA) with key genes in AD pathogenesis. Oral supplementation of B. pseudolongum or PA mimicked TRF's protective effects. Positron emission tomography imaging confirmed PA's blood-brain barrier penetration, while knockdown of the free fatty acid receptor 3 (FFAR3) diminished TRF's cognitive benefits. Notably, we observed a positive correlation between fecal PA and improved cognitive function in an AD cohort, further indicating that TRF enhanced PA production. These findings highlight the microbiota-metabolites-brain axis as pivotal in TRF's cognitive benefits, proposing B. pseudolongum or PA as potential AD therapies. Graphical A 4-month of time-restricted feeding (TRF) intervention alleviated cognitive impairments in Alzheimer's disease (AD) patients, while a 3-month TRF regimen improved spatial memory, reduced amyloid-beta accumulation, and promoted microglial aggregation around plaques in AD mice. Antibiotic-induced gut microbiota depletion partly abolished TRF's benefits. Through creatively integrating gut microbiota, metabolites, and hippocampal genes, Bifidobacterium pseudolongum ( B. pseudolongum ) and propionic acid (PA) were identified as key contributors to TRF's cognitive effects, with supplementation of either mimicking TRF's protective benefits. Positron emission tomography imaging revealed that PA directly crossed the blood-brain barrier, and PA supplementation restored disrupted metabolism in AD mice. Knockdown of its receptor free fatty acid receptor 3 (FFAR3) diminished TRF's protective effects. A case-control study showed a negative association between PA and cognitive status, while the TRF clinical intervention linked fecal PA to cognitive status. These findings suggest PA as a potential biomarker and underscore precise TRF-based nutritional interventions as a promising strategy for managing neurodegenerative diseases. Highlights Time-restricted feeding (TRF) improved cognitive function in Alzheimer's disease (AD) patients, with notable enhancement in executive function. Multi-omics integrated analysis in AD mice identified Bifidobacterium pseudolongum ( B. pseudolongum ) and propionic acid (PA) as key mediators of TRF's cognitive benefits. The potential molecular mechanism by which TRF alleviates cognitive impairment induced by AD involves the B. pseudolongum –PA-free fatty acid receptor 3 (FFAR3) pathway. Case-control study and TRF clinical intervention demonstrated PA as a potential biomarker for AD.
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CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Wenli He,Yaqin Sun,Xu Zhou,Lusheng Huang,Zhilong Xiu
In this study, hydrophobic deep eutectic solvents (HDESs) based on trioctylmethylammonium chloride (TOMAC) and medium-chain fatty acids were investigated for the extraction of carboxylic acids. The effects of molar ratio, extraction temperature, vortex time, pH, and carboxylic acid concentration on the partition behavior of carboxylic acids were evaluated. The natural logarithm of partition coefficients (lnK) for six kinds of mono and five kinds of binary carboxylic acids in the TOMAC-OctA system exhibits an excellent linear relationship to the ClogP of carboxylic acids. Quantum chemical calculations using density functional theory showed that one hydrogen bond is formed between lactic acid (LA) and TOMAC-OctA, while double hydrogen bonds between succinic acid (SA) and TOMAC-OctA. However, extraction of butyric acid (BA) is driven by van der Waals force. Furthermore, analysis of the interaction energy revealed that TOMAC-OctA possesses the highest affinity for the extraction of BA, followed by SA and LA.
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Journal of Functional Foods
·
2024
·
IF 3.8
Shugang Li,Guopeng Li,Dongjie Liu,Mingxin Li,Huanhuan Liu,Wenxiu Zhu,Ke Wang,Xiaomeng Ren
Peach gum polysaccharide (PGP) is a natural compound known for its anti-inflammatory and antioxidant properties. This study aimed to investigate the potential protective effect of PGP against Enterococcus faecium E745-induced pneumonia and elucidate its underlying mechanism. It demonstrated that PGP exert antioxidant effects by activating the Nrf2 signaling pathway, as evidenced by the normalization of MDA, SOD and GSH levels. Notably, PGP inhibited the NF-κB signaling pathway, reduced IL-6 and TNF-α levels in serum, colon and lung, and enhanced the expression of tight junction proteins. In addition, PGP affected the synthesis of short-chain fatty acids (SCFAs), especially increased the content of propionate and butyrate. These results suggest that PGP alleviates pneumonia by restoring the integrity of the intestinal barrier, increasing the production of SCFAs, reducing the inflammatory response and oxidative stress. In conclusion, our study provides valuable insights into the potential application of PGP in the treatment of inflammatory diseases.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
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IF 5.7
Liuying Zhu,Minghui Wang,Hongyan Li,Ting Luo,Zeyuan Deng,Jing Li,Liufeng Zheng,Bing Zhang
3′-Sialyllactose (3′-SL), a major acidic oligosaccharide found in human milk, has been investigated to improve cognitive-enhancing effects with 3 weeks old C57BL/6 mice by administering 3′-SL orally at a dose of 350 mg/kg/day for 6 weeks. Behavioral tests indicated that supplementation with 3′-SL promoted cognitive and memory development in young mice. Through interaction network and coenrichment analysis, nine differentially expressed genes (DEGs) related to memory and cognition were identified and localized in the hippocampal tissue of mice. The intervention of 3′-SL significantly increased the metabolism of sialic acid in mouse hippocampal tissue and promoted the expression of learning-related genes (p < 0.05). Notably, it increased the expression of genes associated with neural cell adhesion molecule (NCAM, p < 0.05), glutamate receptors, and fibroblast growth factor receptor (FGFR, p < 0.05). This suggests that 3′-SL may elevate polysialylated NCAM (PSA-NCAM) levels, which could subsequently interact with FGFR and glutamate receptors, thereby enhancing synaptic growth and plasticity. Additionally, 3′-SL altered the composition of the mouse intestinal microbiota. The synergistic action of gut microbiota and intestinal sialidase promoted the production of free sialic acid, providing essential nutritional elements for the development of the brain’s nervous system. In conclusion, our findings provide new insights into the promoting effect of 3′-SL on cognitive development in growing mice and elucidate its molecular mechanisms.
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