ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 20
·5239-5249
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IF 4.157
Yin Chenhui,Chen Ligang,Niu Na
A sensitive and effective strategy for the detection of cytochrome c (Cyt c) and trypsin was developed using biomass nitrogen-doped carbon quantum dots (N-CQDs) as the fluorescence probe. N-CQDs were synthesized through a one-pot hydrothermal method by utilizing cellulolytic enzyme lignin as the carbon source and ammonia as the solvent and nitrogen source. The obtained N-CQDs had good water solubility and stable optical properties. The introduction of nitrogen increased fluorescence quantum yield (QY) to 8.23%, which was almost four times as high as that before nitrogen doping. The N-CQDs were fabricated as a label-free biosensor to detect Cyt c and trypsin. The fluorescence of N-CQDs was quenched with positively charged Cyt c due to electrostatic induction aggregation and static quenching. However, Cyt c tended to be hydrolyzed into small peptides in the presence of trypsin, which caused fluorescence recovery of the N-CQDs/Cyt c complex. A wide linear response range was achieved for Cyt c within 1–50 μM and the developed N-CQDs/Cyt c complex displayed a linear response for trypsin within 0.09–5.4 U/mL. The detection limits were 0.29 μM for Cyt c and 0.013 U/mL for trypsin, respectively. Furthermore, this assay had been applied to Cyt c and trypsin detection in serum samples with the recoveries in the range of 94.6–98.5% and 95.5–102.0%, respectively. The established method was sensitive, selective, easy to operate, and low cost, which proved its potential application in clinical diagnosis. Graphical abstract
The synthesis and fluorescence mechanism of N-CQDs and the strategy for Cyt c and trypsin detection
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NEW JOURNAL OF CHEMISTRY
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2021
·Vol. 45
·期 27
·12064-12070
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IF 3.591
Ming Zhong,Lingling Li,Kun Zhao,Hui Peng,Shixian Xu,Bitao Su,Dahui Wang
Searching for novel alternatives to the traditional graphite anode in high performance lithium-ion batteries is of great significance, which, however, faces many challenges. In this work, pyrolysis coupled with a selenization strategy was adopted to synthesize a one-dimensional core–shell nanorod composite (MoO2/ZnSe@N-C) by using zeolitic-imidazole framework coated molybdenum trioxide as a precursor. The as-obtained material comprises a zinc selenide loaded molybdenum dioxide core and a nitrogen-doped carbon shell, which can enhance the specific capacity through conversion reactions and buffer the volume change as well as promote the electrical conductivity, respectively. When used as an anode in lithium-ion batteries, the optimized MoO2/ZnSe@N-C sample delivers a high specific capacity (807 mA h g−1 at 100 mA g−1 for 100 cycles) with a coulombic efficiency up to 99%, a good rate capability, and long-term cycling stability for 900 cycles at 500 mA g−1 which is better than that of MoO3 and ZIF-8 derived materials. In addition, the lithium storage mechanism was analyzed by cyclic voltammetry at various scanning rates and the result indicates that the capacitive-controlled behavior dominates the Li+ ion transport kinetics process.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Hangqing Xie,Haogang Zhu,Lei Lei,Xiaolei Yang,Youjie Hua,Renguang Ye,Gongxun Bai,Shiqing Xu
Surface-enhanced Raman scattering (SERS) has attracted much attention with its powerful trace detection and analysis capabilities, especially biological and environmental molecules. However, building a protein SERS detection platform based on semiconductor devices is a huge challenge. Herein, through the synergy of NH 3 and nickel foam, a large-sized semiconductor tungsten oxide hydrate platform (WOHP) was synthesized. The crystal plane of a single WOHP particle is larger than the excitation spot. As a SERS substrate, WOHP can make full use of the excitation light without destroying the structure during the protein molecules detection process. Through the synergy of WOHP and Au NPs, the enhancement factor is 1.5 × 10 4 . Raman peaks of WOHP can be used as references for the detection of typical protein cytochrome C (Cyt C). As the Cyt C concentration decreases, the I Cyt C /I WOHP ratio decreases, and the signal can still be obtained when the concentration is as low as 5 × 10 −9 mol L −1 . More importantly, the method does not affect the catalytic activity of Cyt C and can be applied to the detection of Cyt C concentration in serum.
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RSC Advances
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2021
·Vol. 11
·期 27
·16339-16350
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IF 3.361
Mengkui Ding,Ling Zha,Hui Wang,Jinyao Liu,Peiwu Chen,Yuefeng Zhao,Lan Jiang,Yuhao Li,Ruizhuo Ouyang,Yuqing Miao
A three-dimensional (3D) frogspawn-like structure was achieved by simply coating nano-carbon outside silver nanospheres (Ag@C NFS) and used as a probe to capture the anti-carcinoembryonic antigen for the electrochemical immunosensing of carcinoembryonic antigen (CEA), a typical biomarker of several diseases such as gastric cancer, intestinal cancer and colon cancer. Moreover, Ag@C nanocables (Ag@C NCs) were aslo synthesized. By comparison, the globular 3D frogspawn-like structure endowed Ag@C NFS with a larger surface area, which is preferred to improve the capability of loading antibodies, higher water solubility, better biocompatibility and improved electrical conductivity, which was likely attributed to the synergistic effects of Ag and crystalline graphite carbon and the different structure with more hydroxyl groups exposed. Therefore, the resultant Ag@C NFS was used as an electrochemical immunosensing platform to fabricate a label-free immunosensor for the analysis of CEA, which showed an excellent immunosensing performance with a wide linear CEA detection range from 0.0001 ng mL−1 to 100 ng mL−1 and a low detection limit of 5.12 pg mL−1. In particular, the good reproducibility, high stability and specificity of the proposed immunosensor ensured the successful application in the quantitative determination of CEA in cancerous human serum samples, providing a promising alternative to detect other biomarkers.
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Journal of Materials Chemistry A
·
2021
·Vol. 9
·期 15
·9761-9770
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IF 12.732
Dan Wang,Hao Xu,Peixia Yang,Lihui Xiao,Lei Du,Xiangyu Lu,Ruopeng Li,Jinqiu Zhang,Maozhong An
Extensive efforts have been devoted to active site designing for non-precious metal electrocatalysts; however, the porous structure engineering has been less emphasized, particularly for catalysts for gas diffusion electrodes, e.g., the cathode (oxygen reduction reaction, ORR) of Zn–air batteries. The desired porous structure is not only important for exposing high-density active sites but also beneficial for boosting mass transfer. Here, a dual-template strategy is proposed for constructing hierarchically porous structural Fe–N–C catalysts with dense active Fe–Nx moieties for the cathode of Zn–air batteries. The dual ZnCl2 and MgO hard templates jointly promote the formation of hierarchically interconnected carbon networks. The experimental results indicated both significantly improved active site accessibility and mass transfer, reasonably leading to enhanced ORR activity in conventional three-electrode cells. Particularly, it is deduced that the microporous structure is beneficial for dense active Fe–Nx moieties, while the ultra-macroporous structure is conducive to mass transfer during the ORR process. We further applied this dual-template-mediated catalyst in a real-world liquid Zn–air battery, which presents a high open-circuit voltage of 1.48 V and a remarkable energy density of 952.8 W h kgZn−1. Such robust battery performance is among the best compared with reported Zn–air batteries using non-precious metal catalysts. Particularly, a peak power density of 116.8 mW cm−2 is achieved in an all-solid-state Zn–air battery.
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ANALYST
·
2021
·Vol. 146
·期 14
·4557-4565
·
IF 4.616
Xiangyu Ma,Kun Qian,Onome Ejeromedoghene,Martha Kandawa-Schulz,Wei Song,Yihong Wang
Taking a composite of a nanomaterial and a signal molecule as a substrate material can provide a label-free electrochemical platform. Besides, the nanomaterial with a high catalytic activity towards the signal molecule can improve the sensitivity of the platform. Herein, a thionine functionalized Fe–N–C nanocomposite was employed as the substrate. Firstly, the electrocatalytic activity of Fe–N–C towards the electroreduction of thionine was explored. Then, an immobilization-free and label-free electrochemical platform for the determination of microRNA-21 based on Fe–N–C–thionine/Fe3O4@AuNPs was constructed. A magnetic glassy carbon electrode (MGCE) was used to keep the magnetic Fe–N–C–thionine/Fe3O4@AuNPs modified onto the surface of the MGCE. Fe–N–C and Fe3O4 nanoparticles can co-catalyze the electroreduction of thionine and a strong electrochemical reduction signal of thionine could be realized in the differential pulse voltammetry (DPV) test. Also, a catalytic hairpin assembly (CHA) reaction was utilized to enhance the sensitivity of the developed electrochemical biosensor. Besides, the developed biosensor shows excellent specificity and reproducibility in the test of human serum samples, indicating its wide application prospects in the early-stage diagnosis of tumors.
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MATERIALS CHEMISTRY AND PHYSICS
·
2021
·
IF 4.094
Zhen Xu,Shuyue Yang,Dizi Yang,Dongmei Zhang,Panpan Sun,Shibing Ni
Porous architecture of N-doped carbon coated gallium nitride sub-micrometer bricks comprising of nanoscale carbon-coating primary nanoparticles/reduced graphene oxide hybrids (GaN–NC–RGO) are firstly designed as high-performance anode for lithium-ion batteries (LIBs). The GaN–NC–RGO electrode shows excellent electrochemical performance , with the integral porous architecture and the GaN nanoparticles enhancing the reaction activity, the nanoscale NC coating and the microscale RGO hybridization facilitating electron transfer , and the submicron size of the GaN bricks improving the tap density. The GaN–NC–RGO electrode delivers high discharge capacity of 398.6 mAh g −1 after 550 cycles at 0.05 A g −1 , and this value increases to 445.4 mAh g −1 at 0.02 A g −1 . After 5 periodic rate capability testing over 400 cycles, the discharge capacity of the GaN–NC–RGO electrode can be fully recovered when reverting current to 0.05 A g −1 . The feasible electrode design strategy coupled with the facile fabrication approach and the impressive performances of the GaN–NC–RGO electrode may be referential for the development of high-performance GaN-based materials for LIBs.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Ruzhen Xie,Yanbin Jiang,Andac Armutlulu,Ziye Shen,Bo Lai,Hui Wang
In this work, cost-effective, magnetic carbon-supported Fe@Ti composite (Fe@Ti/Cs) with abundant active sites was synthesized by one-step carbothermal reduction of ilmenite with the assistance of microwave oven and utilized as a highly efficient persulfate (PS) activator for the wastewater purification. The coexistence of Fe 0/2+/3+ , Ti 3+/4+ and oxygen vacancies on Fe@Ti/Cs was found to favor for the electron transfer to PS, which facilitate the generation of reactive oxygen species (ROS). Catalytic experiment results showed that the Fe@Ti/C-4 produced from ilmenite/carbon with a mass ratio of 4:1 exhibited the best catalytic activation performance towards PS for the degradation of Rhodamine B (RhB). Usage of merely 0.12 g/L Fe@Ti/C-4 enabled the removal of 94.01% RhB (200 mg/L) within 30 min in the PS containing system, significantly outperforming ilmenite + PS (29.29%) and carbon + PS (49.91%) systems tested under the same conditions. The physico-chemical properties of the produced Fe@Ti/Cs before and after the reaction were carefully characterized. Radical scavenging experiments and electron paramagnetic resonance (EPR) analysis were carried out to better understand the underlying mechanism. The results indicate that oxygen vacancies in Fe@Ti/C-4 promoted the electron transfer and participated in the transition metal redox cycle to generate ROS in the PS-containing system, which was highly efficient for degrading RhB into small molecules and finally enabling mineralization. This work offers a new perspective for designing highly efficient and stable PS activators with long life derived from natural ore for environmental remediation.
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Scientific Reports
·
2021
·Vol. 11
·期 1
·1-13
·
IF 4.38
Niu Meiyun,Yan Haifeng,Xiong Yuping,Zhang Yueya,Zhang Xinhua,Li Yuan,da Silva Jaime A. Teixeira,Ma Guohua
Sandalwood ( Santalum album L.) is famous for its unique fragrance derived from the essential oil of heartwood, whose major components are santalols. To understand the mechanism underlying the biosynthesis of santalols, in this study, we cloned two related genes involved in the mevalonate pathway in S. album coding for acetyl-CoA C-acetyl transferase (AACT) and 3-hydroxy-3-methyglutary-CoA synthase (HMGS). These genes were characterized and functionally analyzed, and their expression profiles were also assessed. An AACT gene designated as SaAACT (GenBank accession No. MH018694) and a HMGS gene designated as SaHMGS (GenBank accession No. MH018695) were successfully cloned from S. album . The deduced SaAACT and SaHMGS proteins contain 415 and 470 amino acids, and the corresponding size of their open-reading frames is 1538 bp and 1807 bp, respectively. Phylogenetic trees showed that the SaAACT protein had the closest relationship with AACT from Hevea brasiliensis and the SaHMGS proteins had the highest homology with HMGS from Siraitia grosvenorii . Functional complementation of SaAACT and SaHMGS in a mutant yeast strain deficient in these proteins confirmed that SaAACT and SaHMGS cDNA encodes functional SaAACT and SaHMGS that mediate mevalonate biosynthesis in yeast. Tissue-specific expression analysis revealed that both genes were constitutively expressed in all examined tissues (roots, sapwood, heartwood, young leaves, mature leaves and shoots) of S. album , both genes showing highest expression in roots. After S. album seedlings were treated with 100 μM methyl jasmonate, the expression levels of SaAACT and SaHMGS genes increased, suggesting that these genes were responsive to this elicitor. These studies provide insight that would allow further analysis of the role of genes related to the sandalwood mevalonate pathway in the regulation of biosynthesis of sandalwood terpenoids and a deeper understanding of the molecular mechanism of santalol biosynthesis.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 11
·1-10
·
IF 6.232
He Hongliang,Cao Min,Hu Jingwan,Zhu Ling,Su Chang,Du Shuhu,Yang Jian,Tang Yulin,Chen Lina
A novel molecularly imprinted ratiometric fluorescent probe was fabricated by simple sol–gel polymerization for selective and sensitive assay of C-type natriuretic peptide (CNP) in biosamples. Both the nitrobenzoxadiazole (NBD) and carbon dots (CDs) were located on the surface of silica, used as the detection signal and reference signal, respectively. For the turn-on-based probe, the fluorescence intensity of NBD could be quantitatively enhanced by CNP based on the strategy of photo-induced electron transfer (PET), while the fluorescence of CDs remained unchanged. The obtained probe exhibited excellent recognition selectivity and fast kinetics to CNP templates, and also showed good stability. The linear range of CNP determination was 5–80 pg mL −1 with a low detection limit of 2.87 pg mL −1 . Finally, the probe was successfully applied to determine CNP in human serum samples and attained high recoveries between 97.3 and 104% with precisions below 4.7%. The result indicates that the proposed method has promising potential for the assay of trace peptides in complex matrices.
Schematic illustration for the formation and determination mechanism of the probe
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 2
·1-10
·
IF 5.833
Xu Xu,Feng Xue,Liu Zhen,Xue Shan,Zhang Lei
A low cost-effective and simple synthesis method was adopted to acquire three-dimensional flower-like structure Fe 3 O 4 /C that has large specific area, suitable pore structure and sufficient saturation magnetism. The obtained Fe 3 O 4 /C exhibits outstanding preconcentration ability and was applied to extracting non-steroidal anti-inflammatory drugs from complex environmental and biological samples. The parameters of magnetic solid-phase extraction were optimized by univariate and multivariate methods (Box-Behnken design). The high degree of linearity from 2.5 to 1000.0 ng mL −1 (R 2 ≥ 0.9976), the limits of detection from 0.25 to 0.5 ng mL - 1 (S/ N = 3), and the limits of quantitation from 1.0 to 2.0 ng mL - 1 (S/ N = 10) were yielded by adopting this novel method after the optimization. Moreover, the recoveries of non-steroidal anti-inflammatory drugs from 89.6 to 107.0% were acquired in spiked plasma, urine and lake samples. In addition, the adsorption of non-steroidal anti-inflammatory drugs on Fe 3 O 4 /C was explored by adsorption isotherms and kinetic studies. Furthermore, the adsorption mechanism for non-steroidal anti-inflammatory drugs by Fe 3 O 4 /C was proposed, which was hydrogen bonding and π-π interaction between non-steroidal anti-inflammatory drugs and Fe 3 O 4 /C.
Graphical abstract
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ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Xueying Li,Yalin Xu,Hui Wu,Xiu Qian,Lizhuang Chen,Yuanyuan Dan,Qing Yu
The porous Fe 3 O 4 nanoaggregates were fabricated by the carbon polyhedron as templates through the hydrothermal method . The carbon polyhedron was obtained by the carbonization of ZIF-67 and subsequent acid treatment. The finial porous Fe 3 O 4 nanoaggregates possess high specific area of 547.5 m 2 g −1 with pore volume of 0.375 cm 3 g −1 . Such unique structures facilitate electrode materials fully exposed to electrolyte solution and maintain the structural stability during fast electrochemical reaction processes. Thereby, porous Fe 3 O 4 nanoaggregates exhibit high specific capacity of 868.7 C g −1 at 2 A g −1 in the wide potential window of −0.90 - 0.45 V in 3 M KOH. After 3000 cycles at 2 A g −1 , the electrode material retains 771.8 C g −1 . Even at high current density of 20 A g −1 , the electrode still delivers 456.1 C g −1 , which is outstanding of other Fe 3 O 4 nanomaterial for supercapacitor . Furthermore, the Fe 3 O 4 //AC asymmetry supercapacitor (3 M KOH as electrolyte) exhibits high energy density of 24.2 Wh kg −1 at power density of 801.1 W kg −1 with excellent cycling performance.
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