ACS Nano
·
2025
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IF 16
Shuya Wang,Xiaojing Shi,Tingli Xiong,Wenxuan Zheng,Ruishi Li,Weimin Qi,Min Liu,Lin Dai,Min Zhou,Wei Dai,Qiong Huang,Xiaoying Wang,Kelong Ai
The strategy of restoring mitochondrial function by modulating mitochondrial membrane potential (MMP) through uncoupling protein 2 (UCP2) offers significant therapeutic potential against cerebral ischemia-reperfusion injury (CIRI). However, traditional strategies ignored elevation of mitochondrial reactive oxygen species (mtROS) resulting from UCP2 inhibition, resulting in poor therapeutic effects. Here, we reported a multifunctional SGB nanomedicine formed by pioneering the prepolymerization of the UCP2 inhibitor genipin and glycine and a cerebral infarction targeting peptide via a metastable imine bond. After intravenous injection, SGB was highly targeted to affected brain tissue and reached neuronal mitochondria. SGB could not only restore MMP by cleaving the metastable imine bond to release genipin to inhibit overexpressed UCP2, but also simultaneously eliminated excessive mtROS. Compared with traditional UCP2 inhibition, SGB could not only significantly improve the bioavailability of genipin and reduce systemic side effects, but also effectively protected neuronal mitochondria, reduced endoplasmic reticulum stress and inhibited the inflammatory storm of microglia, ultimately significantly reduced neuronal apoptosis. Correspondingly, SGB nearly reversed CIRI with a low 5 mg/kg dose. This innovative approach redefines the role of UCP2 inhibition and provides a framework for the treatment of CIRI by maintaining mitochondrial function.
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高影响因子
LANGMUIR
·
2025
·
IF 3.9
Kaihui Pan,Zexin Zhang,Yang Liu,Yihao Wang,Li Wang,Wantai Yang
In this study, poly(ethylene-alt-maleic anhydride) (PEMA) was selected as the backbone, and PEMA-g-DA/DMAPA was synthesized through an amidation reaction with dopamine (DA) and N,N-dimethylaminopropylamine (DMAPA). The antifogging performance, stability, universality, and antibacterial property after quaternization of the PEMA-DA-DMAPA coating were tested and analyzed. Experimental results showed that the PEMA-DA-DMAPA coating exhibited great antifogging performance, with the water contact angle (WCA) as low as 10.7°. The coefficient of friction of the PEMA-DA-DMAPA coating decreased significantly compared with that of the substrate. Even after 5 days of immersion or 60 cycling tests, the coating still maintained excellent antifogging properties. This coating can be widely applied to the surfaces of transparent substrates, such as poly(methyl methacrylate)(PMMA), polyethylene terephthalate (PET), and polycarbonate (PC). Specifically, the WCA on the PC surface dropped significantly from 89.5° to 11.9°. After quaternization, the coating exhibited notable antibacterial activity against Staphylococcus aureus (S. aureus), with a maximum antibacterial rate of 97.23%.
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Chemical & Biomedical Imaging
·
2025
·
IF 5.7
Zhuohan Jing,Fangdong Long,Yuxiao Xiong,Jiaqi Liu,Zhaoju Li,Haolin Chen,Zhiming Liu,Bo Xu
Mitophagy is closely associated with various diseases. Precise monitoring of its dynamics and understanding its mechanisms are crucial for diagnosing and treating mitophagy-related diseases. This study developed a mitochondria-targeted (MT) surface-enhanced Raman scattering (SERS) nanoprobe to investigate the drug-induced mitophagy through unveiling the mitochondria-related metabolic profiling. The MT-SERS nanoprobe is consist of carbon dots coated with gold nanoparticles functionalized with triphenylphosphonium (Au@CDs-TPP) that exhibits exceptional SERS performance, low cytotoxicity, and mitochondria targeting specificity. Au@CDs-TPP is first used to monitor the classic mitophagy triggered by carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), which demonstrates the diverse metabolic profiling related to mitochondria, like degradation in mitochondrial protein, DNA damage, decreased lipid and cytochrome c content, and increased carbohydrate consumption. We further evaluate the cellular response to anesthetic lidocaine, which can also induce mitophagy in HepG2 cells like FCCP. However, the molecular alterations in lidocaine-induced mitophagy fluctuated differentially compared to that in FCCP-induced mitophagy.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Li Wang,Fan Yang,Jie Gong,Weiwei Chen,Yinbang Zheng,Xi Chen,Weiqing Hu,Yi Li,Ke Yang,Zefeng Wang,Dinggeng He,Luo Hai
Bacterial infections, particularly those caused by multidrug-resistant (MDR) bacteria, pose a serious global health threat. The limited efficacy of traditional antibiotic drugs against MDR strains and the slow development of antibiotics necessitate the exploration of alternative therapeutic strategies. Acid-degradable metallic peroxide has emerged as a promising solution, exhibiting broad-spectrum antimicrobial activity with a reduced risk of resistance development. In this study, we have constructed an acid-responsive bimetallic peroxide nanocomposite (ZCS@M@HA) for the treatment of wound infections involving methicillin-resistant Staphylococcus aureus (MRSA). In our design, the copper-doped zinc peroxide (ZC) nanoparticles are prepared and coated with mesoporous silica to obtain core–shell structured ZC@M, which is subsequently loaded with S-nitroso-N-acetylpenicillamine (SNAP) acting as a nitric oxide (NO) donor and capped with sodium hyaluronate (HA) to prevent premature SNAP release. In the acidic microenvironment of infectious sites, ZCS@M@HA releases Zn2+, Cu2+, and H2O2, and the former disrupts bacterial energy metabolism by impairing the electron transport chain. Meanwhile, self-supplied H2O2 and Cu2+ perform a Fenton-like reaction to generate highly toxic hydroxyl radicals (•OH) for damaging bacterial membranes. Moreover, Cu2+ depletes intracellular glutathione (GSH) to augment the oxidative stress. Furthermore, NO released from SNAP triggered by Cu2+ and GSH accelerates wound healing by promoting collagen deposition, tissue regeneration, and vascularization. In vivo assays demonstrate the high MRSA inhibition efficacy of ZCS@M@HA with optimal wound healing. The proposed acid-responsive ZCS@M@HA with multi-bactericidal modalities offers an effective approach to fight MDR bacteria and presents a promising strategy for treating wound infections.
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ACS Nano
·
2025
·
IF 16
Kexin Li,Xiaojing Zhang,Liqun Xu,Kun Xu,Xi Rao,Selvakumar Murugesan,Valentim A. R. Barão,Peng Yang,En-Tang Kang
Preventing bacterial adhesion and biofilm formation is essential for the long-term success of biomedical implants. Implant-associated infections remain a significant clinical challenge, underscoring the urgent need for effective and durable antimicrobial surface strategies. This study develops a nanocoating with dual antibacterial adhesion and photothermal antibacterial properties for biomedical surface modification. Bovine serum albumin-templated metal sulfide (MS@BSA) nanocomposites are synthesized and converted into a stable nanofilm via phase-transitioned BSA (PTB) self-assembly. The MS@PTB coating adheres to various substrates and demonstrates broad-spectrum antibacterial activity. In vitro assays show that the copper sulfide@PTB (CuS@PTB) coating significantly reduces bacterial attachment and suppresses biofilm development upon 808 nm near-infrared irradiation. RNA sequencing identifies differentially expressed genes in common pathogens, indicating disrupted respiration, energy metabolism, and virulence pathways as well as stress responses to heat and copper ions. In vivo experiments using rat subcutaneous infection and abdominal wall defect models demonstrate that CuS@PTB markedly reduces bacterial load and inflammatory responses while accelerating tissue regeneration and maintaining excellent biocompatibility. The results demonstrate the synergistic antibacterial effects of photothermal heating and Cu ion release, supporting CuS@PTB as a promising antimicrobial surface coating for implantable biomaterials.
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ACS Applied Bio Materials
·
2025
·
IF 4.7
Ying He,Tianze Sun,Jiazhou Wu,Zexian Liu,Yingzhuan Ye,Jie Li,Tao Qian,Xiantong Hu,Jiayi Wang,Xiaomei Bie,Gang Xu,Yantao Zhao
The clinical repair of bone defects is hindered by limitations in donor material and complications arising from autologous bone grafting. Consequently, the development of efficient bone regeneration materials is of great clinical importance. The present study investigated a three-dimensional microporous demineralized bone matrix (DBM) combined with bone marrow mesenchymal stem cells (BMSCs) to enhance the outcomes of bone defect repair. The DBM has been developed to enhance the collagen preparation process, with the aim of retaining the collagen fiber network of the natural bone matrix and forming a three-dimensional microporous structure with good mechanical property. In vitro experiments demonstrated that the biocompatibility of DBM was superior to that of traditional cancellous and cortical bone materials, and they promoted cell adhesion, proliferation, and osteogenic differentiation as well as osteogenesis-related genes. In vivo experimentation was conducted to verify the efficacy of the treatment on a critical-sized cranial bone defect in nude mice. Micro-CT and histological analysis showed more formation of bone at 4 weeks and 8 weeks postsurgery. The DBM with optimized pore structure, degradation rate, and bioactivity enhanced the efficiency of bone defect repair by synergizing the osteogenic activity of BMSCs. The DBM with a porous and cross-linked structure could provide BMSCs with more internal surface area for attachment space to promote cell adhesion and growth and create advantages for the bone formation. Moreover, the DBM contains multifarious osteoinductive growth factors such as transforming growth factor-β (TGF-β), fibroblast growth factors (FGFs), bone morphogenetic proteins (BMPs) and insulin growth factors (IGFs), which can enhance osteogenic differentiation of BMSCs. This study may provide an innovative strategy for bone regeneration and bone defect repair.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Yixuan Li,Qiming Xia,Yiling Li,Haohong Gan,Guoqiao Chen,Xiaolong Liu,Yujie Li,Jing Yang,Jiaxi Cheng,Wei Liu,Yuanyuan Li,Jun Qian,Xiaoxiao Fan,Hui Lin
Solid tumors are susceptible to recurrence due to residual cells persisting postresection, with surgical margin residues posing particular significance. Current adjuvant therapies for preventing recurrence often lack specific targeting capability for the surgical wound site and require repeated administrations, leading to suboptimal efficacy and systemic side effects. In this study, poly(lactic-co-glycolic acid) (PLGA) composite nanofibers embedded with aggregation-induced emission (AIE) luminogens were fabricated via electrospinning. This technique harnesses aggregation-enhanced fluorescence, which could produce second near-infrared (NIR-II) fluorescence and enable photothermal therapy. When implanted at resection sites as a biodegradable patch, the system enables repeated NIR-II fluorescence-guided photothermal treatments, eradicating residual microtumors and thereby reducing recurrence risk. This “single-implant multitherapy” strategy provides a novel approach to postoperative management.
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ACS Nano
·
2025
·
IF 16
Xiaofeng Lu,Dong Zhong,Qi An,Liting Kang,Na Fan,Junjie Cao,Renjie Li,Qianqian Cao,Yudong Zhou,Xiaoyu Du,Shuanghong Yan,Juan Li,Xiaolei Qu,Yuqin Wang
Perfluoroalkyl and polyfluoroalkyl substances (PFASs) make up a large class of emerging chemical pollutants that have caused extensive contamination of global water sources. As the toxicity of PFAS becomes increasingly recognized, there is a growing demand for low-cost and rapid sensors capable of screening water samples for multiple PFAS species. However, most electrochemical and optical sensors can detect only one or two PFASs, despite the high structural diversity of these compounds in aquatic environments. Here, we report a single-molecule nanopore sensor that enables simultaneous detection of nine PFASs in a single measurement. By incorporating a β-cyclodextrin (β-CD) adapter into a mutant α-hemolysin (α-HL) nanopore, translocating PFAS molecules produce distinct current blockades that allow clear discrimination based on carbon chain lengths, hydrogen substitutions, and terminal functional groups. With assistance from a machine learning classifier, an overall identification accuracy of 95.83% is achieved. This strategy allows direct, label-free, and rapid discrimination of multiple PFASs in surface water samples at environmentally relevant concentrations as low as the microgram per liter level, without chemical labeling, separation, or enrichment. The successful demonstration of nanopore sensing in complex real-world matrices highlights its strong potential for practical, field-deployable environmental analysis.
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高影响因子
LANGMUIR
·
2025
·
IF 3.9
You Zhou,Zhengkai Han,Yunjin Li,Yun Xue,Jingshuang Zhang,Xinyue Zhang,Rui Shi
The inherent bioinertness of titanium alloy presents significant challenges for implants, including poor cell adhesion, restricted proliferation, and differentiation, ultimately hindering efficient osseointegration. To address this limitation, this study introduced a magnesium-incorporated gelatin-based macroporous hydrogel (Gelatin-Mg) bioactive interface. This hydrogel modified the surface of porous titanium alloy scaffolds, enhancing osseointegration through a dual mechanism: (1) promoting cell adhesion and proliferation on the scaffold surface and (2) synergistically leveraging magnesium ions to boost osteogenic and angiogenic capabilities. These combined actions culminated in the enhanced ingrowth of new bone tissue within the titanium scaffold, enabling effective osseointegration. The bioactive hydrogel interface demonstrated suitable mechanical properties, sustained magnesium ion release kinetics, and excellent biocompatibility. It significantly promoted both osteogenic and angiogenic performance in in vitro cell assays. In rat bone defect models, compared with unmodified scaffolds, scaffolds modified with the Gelatin-Mg bioactive interface exhibited superior osseointegration efficacy. Therefore, this bioactive hydrogel interface could provide a robust material platform for achieving precise and effective osseointegration, offering significant potential for improving fracture healing outcomes.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yajuan Yan,Jialian Ding,Wenxuan Fu,Lurong Ding,Wei Hu,Guangzhong Ma,Bin Su
Immunotherapy using activated T cells as the weapon for controlling and eliminating tumor cells represents a promising therapeutic strategy for cancer treatment, in which accurate detection of T cell activation is critical for evaluating the immunotherapy efficacy. Activated T cells upregulate specific plasma membrane biomarkers, such as CD69, that can act as an indicator of activation status. In this work, we report a novel strategy of assessing T cell activation by electrochemiluminescence (ECL) imaging of CD69 expression on the plasma membrane. Mesoporous silica nanoparticles functionalized by polyethylenimine (PEI-SiO2) were prepared as both “nanocoreactant” and “nanoreactor” to remarkably enhance the ECL reaction through a dual-pathway scheme, in which both the catalytic route and the low-oxidation-potential route contribute to produce an enhanced ECL signal. Based on this strategy, subsequent conjugation of PEI-SiO2 with anti-CD69 antibodies allowed us to detect individual CD69 proteins by single-molecule ECL imaging, visualize their spatial distribution on the plasma membrane of activated T cell, and reveal their expression dynamics. The results establish a novel dark-field imaging strategy for assessing T cell activation, offering a low-background tool for investigating immune cell dynamics and immunotherapy efficacy through single-cell-level analysis of key biomarkers.
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JOURNAL OF MEDICINAL CHEMISTRY
·
2025
·
IF 6.8
Hao Tian,Quan Zuo,Siqi Zhang,Hongyi Huang,Dengfu Wu,Zihan Huang,Xiangding Liu,Lin Xie,Yiding Zhang,Hailong Zhang,Yekuan Shi,Suping Li,Ming-Rong Zhang,Rui Wang,Kuan Hu
The development of ideal peptide-based radiopharmaceuticals faces critical bottlenecks, primarily due to limited cellular internalization and insufficient deep tissue penetration of peptide carriers. To address this, we developed an intracellular targeting and DNA-adjacent radiotherapeutic strategy using stapled peptides. The MDM2/MDMX-targeting stapled peptide-based radiopharmaceuticals, denoted as [64Cu]Cu-DOTA-STP, exhibited prolonged circulation in the bloodstream and slow systemic clearance. Furthermore, in vitro studies demonstrated that nearly 50% of administered [64Cu]Cu-DOTA-STP was internalized, achieving efficient intracellular accumulation. In addition, [64Cu]Cu-DOTA-STP demonstrated high tumor accumulation, with a standard uptake value of up to 9.39 ± 1.52%ID/g. Finally, targeted radionuclide therapy confirmed that [64Cu]Cu-DOTA-STP effectively inhibited tumor growth, irrespective of p53 phenotypes. Taken together, this study leveraged PET imaging as a noninvasive and longitudinal tool to elucidate the in vivo fate of stapled peptides and demonstrated that stapled peptides can serve as ideal vehicles for developing intracellular protein-targeting radiopharmaceuticals, achieving efficient 64Cu-based targeted radionuclide therapy.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Tianyang You,Zhijia Wang,Shiyuan Yang,Jitao Wang,Yuan Ding,Lufei Zheng,Xiude Hua
Immunoassays are reliable methods for pesticide residue detection, where antibody quality determines assay performance. In this study, we synthesized four haptens and generated 17 monoclonal antibodies (mAbs) against chlorpyrifos (CHP) using an optimized electrofusion. Through negative sorting of B lymphocytes and treatment with the optimal inducer (10 μg/mL interleukin-4 and 2.5 ng/mL lipopolysaccharide), the electrofusion rate was increased to 0.206%, which is 10-fold higher than that of conventional electrofusion. An AuNP-based lateral flow immunoassay (AuNP-LFIA) developed with mAb 7A2F8E5 achieved a visual detection limit of 1.25 ng/mL for CHP in buffer and 0.005 to 0.02 mg/kg in various crops, all below the maximum residue limits of the U.S., Japan, and China. The accuracy of the AuNP-LFIA was further validated through UPLC-MS/MS. These findings demonstrate a novel and efficient electrofusion strategy for generating high-affinity mAbs, and establish a rapid, sensitive, and reliable AuNP-LFIA for practical monitoring of chlorpyrifos residues in agricultural products.
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