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iScience · 2023 · IF 5.8

Overexpression and knockdown of cotton GhdadD gene reveals its drought and salt stress tolerance role

Yuanyuan Zhang,Jie Zheng,Shiraku Margaret Linyerera,Richard Odongo Magwanga,Yuqing Hou,Yuhong Wang,Yanchao Xu,Aziz Khan,Shuxun Yu,Zhongli Zhou,Fang Liu,Xiaoyan Cai

Summary The 5′-deoxyadenosine deaminase (DADD), a member of the amidohydrolase family regulates biological purine metabolism. In this study, bioinformatic analysis, overexpression and knockdown of GhdadD gene were detected to identify its potential role in drought and salt stress tolerance. The results revealed that GhdadD was induced by ABA, Auxin, MBS and light responsive elements. In transgenic Arabidopsis, seed germination rate and root length were increased under drought or salt stress. GhdadD overexpressed seedlings resulted in higher plant height, less leaf damage and lower ion permeability. The expression of osmotic stress and ABA-responsive genes were up regulated. While in GhdadD -silenced cotton seedlings, CAT, SOD activity and soluble sugar content were reduced, MDA content was increased, and the stoma opening was depressed under drought or salt stress. Some osmics stress marker genes were also up regulated. These data indicating that GhdadD enhanced plant resistance to drought and salt stress through ABA pathways.

✓ 已验证 产品关联 DOI PubMed 高影响因子
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Case Studies in Thermal Engineering · 2023 · IF 6.8

Experimental study on thermophysical property of deep eutectic solvents as heat transfer fluid

Jing Fan,Dazhi Gao,Fenhong Song

Deep eutectic solvents are now widely used in metal electrochemical deposition, biomass catalysis and material synthesis because of their advantages of greenness, low cost, good biocompatibility and biodegradability, and it is proved that DESs have great potential for its utilization as heat transfer fluids. In this work, eight DESs were prepared by thermal method using choline chloride as the hydrogen bond acceptor and glycerol, polyethylene glycol 600, lactic acid and o-cresol as the hydrogen bond donor. Based on the flow calorimetry method, the specific heat capacity system was established and its reliability and precision were verified by measuring the specific heat capacity of deionized water. The maximum deviation of the experimental value and the standard value under the pressure 101 kPa was 2.03%, and the average absolute deviation was 1.01%, and the repeatability was less than 1.5%. Then, the densities and the constant pressure specific heat capacities of eight DESs in the temperature range of 293 K–353 K and 293 K–373 K were measured respectively. The experimental results could support for the scale utilization of DESs.

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SCIENCE OF THE TOTAL ENVIRONMENT · 2023 · IF 9.8

Investigation of the acicular aragonite growth behavior in AOD stainless steel slag during slurry-phase carbonation

Ya-Jun Wang,Jun-Guo Li,Meng-Jie Tao,Xi Zhang,Jian-Bao Zhang,Song Qin,Shao-Hua Liu,Li-Jie Peng,Xiao-Pei Zhang,Ya-Nan Zeng

This study presents a novel method for producing acicular aragonite using argon oxygen decarburization (AOD) slag while controlling the reaction temperature, reaction time, stirring speed, and the magnesium-to‑calcium stoichiometric ratio. This approach provides steel plants with an opportunity to decrease their CO 2 emissions and promote efficient resource utilization and CO 2 storage through the production of high-quality value-added products. The experimental results showed that reaction temperature was the most significant factor affecting the carbonation efficiency of AOD slag, followed by reaction time, stirring speed, CO 2 partial pressure, and the liquid-to-solid ratio (L/S). The study also found that elevated temperature and prolonged reaction duration favored the preferential precipitation of aragonite. Additionally, raising the temperature and the magnesium-to‑calcium stoichiometric ratio was shown to enhance the formation of aragonite, affecting its crystal growth orientation and dimensions. The optimal combination of reaction parameters for the preparation of acicular aragonite was found to be the reaction time of 8 h, the magnesium-to‑calcium stoichiometric ratio of 0.8, the reaction temperature of 120 °C, and the stirring speed of 200 r·min −1 . Under these conditions, the resulting acicular aragonite exhibited excellent overall uniformity, a large aspect ratio, and a smooth crystal surface, with a content of 91.49 %, a single crystal length ranging from 9.86 to 32.6 μm, and a diameter ranging from 0.63 to 2.15 μm. This study provides valuable insights into the efficient production of acicular aragonite from steel slag while reducing CO 2 emissions and promoting the sustainable use of resources.

✓ 已验证 产品关联 DOI PubMed 高影响因子
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2023 · IF 8.2

Alginate based films integrated with nitrogen-functionalized carbon dots and layered clay for active food packaging applications

Long Mao,Juan Zuo,Yujie Liu,Bowen Zheng,Xianglong Dai,Zeqing Bai,Yulin Liu,Jin Yao

The applications of alginate derived from seaweed polysaccharide in food packaging are restricted due to their inherent deficient antibacterial, antioxidant and UV barrier properties. In this study, nitrogen-functionalized carbon dots (NCDs) with active functions (0.5–3 %) and layered clay (1 %) with barrier property were introduced to construct alginate based active films via solution casting method. The results showed that the synthesized spherical NCDs had a particle size of 2–3 nm, and the internal structure of NCDs was similar to graphene, with a large number of active groups (-NH 2 , -OH, etc.) on the surface. Infrared analysis revealed that NCDs could form strong hydrogen bonds with alginate matrix, which slowed down the deterioration of mechanical properties and reduced the surface wettability . With the addition of NCDs, active functions and surface hydrophobicity of the active films were enhanced significantly ( P  < 0.05). When the amount of NCDs reached 3 %, UV barrier, antioxidant and antibacterial properties of the active films were increased by 50.0 %, 61.1 % and 70.1 %, respectively. The addition of NCDs could enhance the anti-browning ability of alginate based coatings and extend the shelf life of banana significantly. Therefore, a suitable amount of NCDs (1–2 %) and layered clay (1 %) can synergistically improve comprehensive performance of alginate based films and promote their food packaging application used as active films/inner coatings.

✓ 已验证 产品关联 DOI PubMed 高影响因子
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SOLAR ENERGY MATERIALS AND SOLAR CELLS · 2023 · IF 6.9

CQD-ATO hybrid nanofluid with good stability in the application of spectral beam splitters

Ammar Dmour,Yang Xiao,Wenshuang Tian,Guangzhao Qin,Xiong Zheng

In photovoltaic power systems , nanofluids are promising candidates for spectral beam splitters (SBSs) owing to their excellent spectral absorption regulation characteristics. The drawbacks of high cost and instability restrain the practical application of nanofluid SBSs. Here, we proposed a hybrid nanofluid SBS containing carbon quantum dots (CQDs) and antimony tin oxide (ATO) nanoparticles , which has been proven to be an excellent SBS from multiple perspectives. Both CQDs and ATO nanoparticles have low cost and super stability, which ensures the long-term practical application of hybrid nanofluid SBSs. Then, the light absorption characteristics of hybrid nanofluids can be effectively regulated because of the complementary optical properties of CQDs and ATO nanoparticles. Furthermore, the PV/T conversion model and indoor test were used to assess the filtering performance of hybrid nanofluid SBSs. The results indicate that CQD-ATO hybrid nanofluids can achieve a high merit function value of 1.815, which is significantly higher than pure CQD nanofluids and outperforms other nanofluid SBSs reported in the literature. This paper may promote the practical utilization of nanofluid SBSs in photovoltaic power systems.

CAS / MDL: 56-81-5 25322-68-3
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2023 · IF 8.2

Development of seaweed-derived polysaccharide/cellulose nanocrystal-based antifogging labels loaded with alizarin for monitoring aquatic products' freshness

Huan Li,Fan Jiang,Jian Chen,Yanbo Wang,Zhigang Zhou,Renjie Lian

Intelligent freshness indicator labels have attracted great interest for their massive potential in monitoring the freshness of aquatic products over the years. However, there is still a challenge where fogging on the labels during dramatic temperature changes affects the reading of freshness. At the same time, the freshness indicator labels need high mechanical strength to resist collision damage during transportation and storage. Herein, an antifogging freshness indicator label was developed based on seaweed extracts and alizarin. Firstly, soluble polysaccharides and insoluble components were extracted from Gelidium amansii , and cellulose nanocrystal (CNC) was further prepared from the insoluble components by sulfuric acid hydrolysis . Subsequently, a polysaccharide-based film was fabricated using soluble polysaccharides as the matrix materials and CNC as the reinforcement agent. Antifogging experiments showed that the hydrophilic composite films presented good antifogging performance. After loading with alizarin, the composite indicator label exhibited both antifogging and freshness-indicating properties for the salmon sample. The work provided a new idea for developing freshness indicator labels suitable for low-temperature transportation and storage.

✓ 已验证 产品关联 DOI PubMed 高影响因子
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Journal of Environmental Chemical Engineering · 2023 · IF 7.7

Lower-temperature preparation of porous Al2O3 supports for O/W separation membranes by recycling mixed-dimensional attapulgite clay as sintering aid

Hengyang Mao,Hengqi Shi,Zhijie Zhang,Shouyong Zhou,Minghui Qiu,Mingmin Jia,Meisheng Li,Yijiang Zhao,Shanshan Xia,Zhentao Wu

Ceramic membranes have drawn attention in oil-in-water (O/W) treatment due to natural hydrophilicity and excellent chemical stability, however their application was restricted by high cost. In this work, a cost-effective Al 2 O 3 -based ceramic support was prepared at sintering temperature of 1500 °C with low-cost mixed-dimensional attapulgite (MDATP) as aid. The composite support with addition of 10 wt% MDATP and 3 wt% powdered carbon possessed an average pore size of 3 µm, bending strength of 63.7 MPa, pure water permeability of 1.32 × 10 4 L·m −2 ·h −1 ·bar −1 . Mullite was formed due to the reaction between Al 2 O 3 and SiO 2 in MDATP during sintering, which played a key role in preparing support with corrosive resistance performance. Then, a superwetting microfiltration (MF) membrane with pore size of 0.18 µm and permeability of 1470 L·m −2 ·h −1 ·bar −1 was obtained by coating ATP nanofiber on the Al 2 O 3 -MDATP support with sintering temperature of 600 °C. The molecular dynamics results showed that the ATP can repel oil fouling with interaction energy of − 872 Kcal·mol −1 . The excellent O/W emulsion separation efficiency of the MF membrane further demonstrated the feasibility of ATP and its by-products in decreasing membrane cost and improving performance.

CAS / MDL: 56-81-5 9002-89-5
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PROGRESS IN ORGANIC COATINGS · 2023 · IF 6.6

Improving the shelf life of tofu using chitosan/gelatin-based films incorporated with Pulicaria jaubertii extract microcapsules

Qais Ali Al-Maqtari,Talal Abdulrazzak Ali Alkawry,Kabirou Odjo,Adel Ali Saeed Al-Gheethi,Mohamed Ghamry,Amer Ali Mahdi,Waleed Al-Ansi,Weirong Yao

This study aimed to improve the shelf life of fresh tofu (FT) by applying chitosan (CH) and/or gelatin (GEL) films loaded with two concentrations of microcapsules containing Pulicaria jaubertii extract (MPE). The effect of packaging on the shelf life of FT was evaluated based on physicochemical, microbiological, and textural properties and bioactive compounds during storage for 4 days at 25 °C. In comparison with the films without MPE, the films containing MPE were more effective at extending the shelf life of FT, and those with 2 % MPE concentration achieved optimum results. The FT packaged by CH:GEL film containing 2 % MPE had a positive effect on the studied properties of FT during the storage period, with optimum values in weight loss (47.96 %), moisture content (23.09 %), pH (5.2), acidity (0.049 g lactic acid/100 g FT), hardness (1.74 N), chewiness (1.14), and cohesiveness (0.87 N) after 4 days of storage, whereas the FT packaged by CH film containing 2 % MPE showed less change in free amino acid combinations and had the lowest count of coliform (2.69 log/g FT), E. coli (1.77 log/g FT), Staphylococcus spp. (1.98 log/g FT), and total fungi (4.95 log/g FT) after 4 days of storage. The FT packaged with GEL film containing 2 % MPE showed the highest total phenolic content (2.39 mg gallic acid equivalent/g FT) and the highest scavenging values of DPPH (139.52 ± 3.45 μg ascorbic acid/g FT) and ABTS + (228.71 ± 1.11 μg Trolox equivalents/g FT) on day 4. However, CH and/or GEL films with MPE led to the formation of high-quality films that can effectively prolong the shelf life of FT.

CAS / MDL: 9012-76-4 56-81-5
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BIOSENSORS & BIOELECTRONICS · 2023 · IF 12.6

A L-glutamine binding protein modified MNM structured optical fiber biosensor based on surface plasmon resonance sensing for detection of L-glutamine metabolism in vitro embryo culture

Jianfeng Chen,Liyun Ding,Jue Zhao,Xingdong Jiang,Fei Ma,Haijun Li,Yumei Zhang

A surface plasmon resonance (SPR) optical fiber sensor with multimode-coreless-multimode (MNM) structure was developed, which modified by L-glutamine-binding protein (QBP) for detection of L-glutamine (Gln). The QBP was immobilized on the surface of gold films by chemical cross-linking and exhibited a binding affinity for L-glutamine. The conformation of QBP can be changed from the “open” to the “closed”, which led to a red-shift of the SPR peak when QBP bounded to L-glutamine. There was a good linear correlation between is a dependence of the SPR peak on and the concentration of L-glutamine concentration in the range 10–100 μM, with a sensitivity of 10.797nm/log 10 [Gln] for L-glutamine in the in vitro embryo culture (IVC) medium environment, and the limit of detection (LOD) is 1.187 μM. This QBP-modified MNM structure optical fiber SPR sensor provides a new idea for the developmental potential assessment of embryos in the process of in vitro embryo culture.

✓ 已验证 产品关联 DOI PubMed 高影响因子
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JOURNAL OF PHARMACEUTICAL SCIENCES · 2023 · IF 3.8

Tacrolimus-Loaded Cationic Nanoemulsion In-Situ Gel System: In-Vitro Characterization and Performance in a Dry-Eye Rabbit Model

Qiuhe Wang,Zheng Wu,Feifei Wang,Hua Zhang,Li Gan

Dry eye disease (DED) is a highly prevalent ocular surface disease that affects life quality and reduces productivity at work. The purpose of this study is to improve the efficacy of tacrolimus (FK506) in the treatment of DED using the special eye surface retention properties of cationic nanoemulsion (CNE) modified by thermosensitive in-situ gel (ISG) (CNE-ISG). The precorneal retention of CNE-ISG containing 0.05% FK506 (50 min) was longer than that of CNE containing 0.05% FK506 (25 min) and commercial suspension containing 0.1% FK506 (Talymus®) (10 min). Successfully modeled dry-eye rabbits were treated with 0.05% CNE-ISG (twice/day), 0.05% CNE and 0.1% suspension (Talymus®) (thrice/day). Schirmer's tear secretion test showed no significant difference between the CNE-ISG group and the healthy group after 5 days of treatment ( p  > 0.05). The results of a tear ferning test (TFT) showed that the tear-fern-like crystal branches in the CNE-ISG group returned to normal after 5 days of treatment. Histological analysis showed that the number of goblet cells in the CNE-ISG group significantly increased. HET-CAM stimulation test showed that the CNE-ISG group had no ocular irritation. The above results indicated that CNE-ISG might be a promising delivery system and as an effective dosage form was employed for FK506 in the treatment of DED.

CAS / MDL: 56-81-5 25301-02-4
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APPLIED SURFACE SCIENCE · 2023 · IF 6.7

Macroscopic ultra-low friction and wear enabled by carboxylated graphene with glycerol

Yangqin Liu,Lei Chen,Lin Liu,Pengfei Shi,Junhui Sun,Yang Wang,Linmao Qian

Reducing wear and even achieving superlubrication in industrial applications using simple yet effective methodologies has been a longstanding research objective. In this study, we propose a convenient approach by employing a combination of carboxylated graphene (Gr-COOH) coating with glycerol as lubricants. Compared to the non-lubricated state, this strategy enabled the achievement of superlubrication with an exceptionally low friction coefficient of 0.002, while simultaneously reducing wear volume of substrate by 4 orders of magnitude, and the wear of the counterpart balls are nearly zero. The transfer of Gr-COOH to the ball surface led to the formation of a low shear resistance interface with the Gr-COOH present on the substrate. Nanoscopic experiments with atomic force microscopy revealed that the substrate surface lubricated by this method exhibited more hydrophilic properties. The formation of a featured hydrophilic interface enables the persistence of glycerol molecules in the contact region for an extended duration, thereby facilitating continuous elastohydrodynamic lubrication. This proposed lubrication methodology holds broad utilization potential as a wear-reduction strategy across various industrial fields.

CAS / MDL: 56-81-5
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INDUSTRIAL CROPS AND PRODUCTS · 2023 · IF 5.9

Elucidating multi-scale deconstruction of bamboo crystalline cellulose by novel acetylcholine chloride based deep eutectic solvents for enhanced enzymatic hydrolysis

Zhe Ling,Yang Tan,Xinjian Li,Ying Xie,Peng Wang,Yan Su,Qiang Yong

Exploration and application of novel and sustainable deep eutectic solvents (DESs) on lignocellulose pretreatment are promising approaches to efficient bioconversion . However, comprehensive understanding on efficient DESs selection and inner mechanisms of lignocellulose deconstruction are still limited. Herein, bio-based acetylcholine chloride (AChCl) was firstly utilized as hydrogen bond acceptor in cooperation with lactic acid, urea and glycerol respectively for microwave-assisted pretreatment on moso bamboo in short time. High enzymatic conversion yield of bamboo cellulose (∼85%) was achieved by lactic acid-AChCl DES due to its lower pH value and viscosity, followed by glycerol-AChCl and urea-AChCl DES. In addition to the features of proposed DESs, changed chemical and physical properties of highly recalcitrant bamboo biomass after pretreatments were systematically determined. Besides of great amount of lignin (∼90%)/ xylan (100%) removal, micro- to nano-scale variations of cellulose structures including more dissociated microfibrils , lowered crystallinity, and enlarged crystallite sizes all played key roles in promoting bioconversion yields. Moreover, the multi-factors contributing to improved hydrolysis performance were integrated to evaluate the effects of novel DESs pretreatments on lignocellulose deconstruction, which may provide new strategies on bioconversion enhancement and process optimization.

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