ACS Applied Nano Materials
·
2024
·
IF 5.3
Qiuxiang Li,Guowei Zhao,Fengling Wu,Xilan Tang,Zhenggen Liao,Xinli Liang,Wei Dong
Imperatorin (IMP) has demonstrated potential in reversing drug resistance in various tumor cells; however, its limited clinical application and development are attributed to its poor water solubility and low bioavailability. The current work is intended to enhance the oral bioavailability of IMP and its efficacy in overcoming tamoxifen (TAM) resistance in breast cancer cells. We employed mPEG–PLLA and Cremophor RH60 (RH60) to prepare imperatorin nanomicelles (IMP-RH60/mPEG–PLLA). The freeze-dried powder of IMP-RH60/mPEG–PLLA demonstrated a porous structure, and upon reconstitution, the resulting solution exhibited clarity and transparency. The particle size was measured to be 25.48 ± 0.92 nm, with a zeta potential of −5.50 ± 1.06 mV. The encapsulation efficiency was determined to be 85.78 ± 0.84%, while the drug loading was found to be 2.66 ± 0.03%. In comparison to the active pharmaceutical ingredients (APIs) of IMP, IMP-RH60/mPEG–PLLA exhibited a significant increase in in vitro release, improved bioavailability in rats, and enhanced uptake by MCF-7/TAM cells. The coadministration of IMP-RH60/mPEG–PLLA and TAM effectively inhibited the proliferation, colony formation, migration, and invasion of MCF-7/TAM cells. This outcome could potentially be attributed to the downregulation of GST-π and MDR1 drug resistance genes, consequently augmenting the susceptibility of MCF-7/TAM cells to TAM. An experimental model of BALB/c nude xenograft, consisting of human breast cancer cells resistant to tamoxifen (TAM), was established in order to assess the potential in vivo reversal of TAM resistance through the use of IMP-RH60/mPEG–PLLA. The growth of xenografts was inhibited by IMP-RH60/mPEG–PLLA, and it induced apoptosis in tumor tissues through the downregulation of Ki-67, GST-π, and MDR1 gene expression. The findings indicated that the utilization of IMP-RH60/mPEG–PLLA micelles resulted in an enhancement of IMP’s bioavailability and its efficacy in overcoming TAM resistance.
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2024
·
IF 6.9
Jingmou Yu,Yifei Zhang,Meilin Xu,Dengzhao Jiang,Wenbo Liu,Hongguang Jin,Pu Chen,Jing Xu,Lei Zhang
Herein, we constructed innovative reduction-sensitive and targeted gelatin-based micelles for doxorubicin (DOX) delivery in tumor therapy. AS1411 aptamer-modified gelatin-ss-tocopherol succinate (AGSST) and the control GSST without AS1411 modification were synthesized and characterized. Antitumor drug DOX-containing AGSST (AGSST-D) and GSST-D nanoparticles were prepared, and their shapes were almost spherical. Reduction-responsive characteristics of DOX release in vitro were revealed in AGSST-D and GSST-D. Compared with non-targeted GSST-D, AGSST-D demonstrated better intracellular uptake and stronger cytotoxicity against nucleolin-overexpressed A549 cells. Importantly, AGSST-D micelles showed more effective killing activity in A549-bearing mice than GSST-D and DOX⋅HCl. It was revealed that AGSST-D micelles had no obvious systemic toxicity. Overall, AGSST micelles would have the potential to be an effective drug carrier for targeted tumor therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Zhihao Li,Dihong Zhong,Haipeng Lin,Peiyin Li,Yongyi Li,Qingmei Huang,Lingzhi Yang,Xueji Zhang
Solid-phase microextraction (SPME) is essential for analyzing ultra-low concentration samples, such as trace drugs and environmental pollutants. Among various formats, pipette tip-based SPME (PT-SPME) stands out for its advantages in efficiency, automation, and flexibility. However, PT-SPME faces challenges such as material loss, high synthesis costs, and environmental impact. To address these issues, we developed a novel PT-SPME device by synthesizing Schiff-base covalent organic frameworks (COFs) in situ on diatomaceous earth (DE), which served as both a matrix and support. This approach significantly improved stability and reduced COFs consumption by 93.7 %. The DE@COFs were packed into pipette tips with a sandwich-like structure and used to extract polycyclic aromatic hydrocarbons (PAHs), with detection achieved through high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD). The method exhibited excellent sensitivity, with limits of detection (LODs) ranging from 2.05 to 52.5 pg/mL and limits of quantification (LOQs) from 15.0 to 159 pg/mL. It also demonstrated high accuracy, with recoveries between 87.05 % and 115.86 %, and strong repeatability, making it suitable for trace pollutant analysis in complex matrices This cost-effective and environmentally friendly PT-SPME system enhances extraction efficiency while reducing material usage, providing a practical and innovative solution for trace pollutant analysis in pharmaceutical and environmental applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2024
·Vol. 26
·期 6
·3211-3220
·
IF 9.3
Pengfei Li,Yehong Han,Dandan Han,Hongyuan Yan
An innovative solid-phase microextraction (SPME) fiber coating, melamine–formaldehyde resin (MFR), was synthesized with a green cross-linker (paraformaldehyde), and it exhibited high heat resistance (up to 350 °C). Seven polycyclic aromatic hydrocarbons (PAHs) were chosen as analytes, and the results demonstrated the MFR-based SPME fiber's exceptional enrichment capabilities and efficient extraction, with high enrichment factors and significantly improved efficiency compared to the conventional polydimethylsiloxane (PDMS) fibers. The unique superiority of the MFR was attributed to its strong π–π interactions and amino-associated enhancement, as well as its porous structure and rough surface, which provided abundant adsorption sites and facilitated rapid mass transport of analytes. Furthermore, the preparation conditions of the MFR-coated SPME fiber were compared with those of commercially available and reported fibers, emphasizing the advantages of the MFR-coated fiber, such as its simplicity, cost-effectiveness, reproducibility, and eco-friendliness. The MFR-based SPME fiber exhibited significant advantages, including high enrichment factors (ranging from 1906- to 7153-fold) for PAHs and good fiber-to-fiber reproducibility (8.7–14.6%), outperforming commercially available PDMS fibers by a factor of 4.9–82.8-fold. This study also considered important factors affecting the extraction process, such as ionic strength, temperature, and time, and optimized these parameters for the best extraction efficiency. The greenness of the developed method was assessed using the Analytical Eco-Scale, and the results showed that it was green in terms of reagent dosage, energy consumption, and waste. The developed headspace solid phase microextraction–gas chromatography–tandem mass spectrometry (HS-SPME-GC-MS/MS) method based on the MFR coating exhibited excellent precision, accuracy, and repeatability, making it a reliable method for the determination of trace PAHs in environmental water samples.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Min Sun,Jiaqing Feng,Yang Feng,Xubo Xin,Yali Ding,Juanjuan Feng
Background Because of the advantages of good selectivity , high sensitivity, and fast analysis, high performance liquid chromatography (HPLC) has become one of the modern analytical techniques in wide application range, such as biological analysis, environmental detection, pharmaceutical and food inspection, agriculture and other fields. The stationary phase greatly decides the chromatographic separation performance, so the development of novel stationary phase is most important for HPLC. Results Pyridyl conjugated microporous polymers (P-CMP) with one to four layers were modified on the surface of amino silica to obtain a novel core-shell material (SiO2@P-CMP) by the layer-by-layer assembly strategy and Chichibabin reaction . The relationship between the structure of SiO2@P-CMP and chromatographic performance was carefully investigated, and the retention mechanism was revealed. The interactions including π-π stacking, hydrophobic effect and hydrogen bond gradually enhanced with the increase of P-CMP layers on the silica surface. Compared with C18 column, SiO2@P-CMP columns displayed better separation selectivity for polycyclic aromatic hydrocarbons (PAHs). According to the relative retention values ( α ), the separation performance of SiO2@P-CMP columns ( α = 1.144–1.884) for PAH isomers and other analytes was obviously better than that of C18 column ( α = 0.998–1.487). Furthermore, the SiO2@P-CMP column with four layers was selected to separate different types of analytes (eight PAHs, four bisphenols , four estrogens and nine phthalates), and the peak order of analytes was different from that on the C18 column due to the influence of hydrogen-bonding and π-π interactions. The relative standard deviations (n = 10) of retention time and peak area on SiO2@P-CMP column were between 0.28 % and 1.98 %. Significance and novelty Pyridyl conjugated microporous polymer was introduced as the stationary phase for the first time in HPLC. The proposed column displayed better separation characteristics compared to Zorbax SB-C 18 column. It provided a new idea for the separation of small molecules and the development of chromatographic packing or extraction material.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2024
·
IF 4.7
Hu Haotian,Liu Hongjing,Li Qingqing,Shen Yongpeng,Lu Jialin,Liu Xiaowen,Tong Yu,Feng Yiheng,Xu Qingbo,Tang Yuxiang,Chu Jing,Hu Hang,Zhu Tianyu,Xu Defeng
Polymer-based nanoparticles with tumor-targeting ability, controlled-release properties and good biocompatibility are of great interest for anticancer drug delivery. Herein, two amphiphilic reduction-responsive copolymers self-assembled nanoparticles (mPEG-Cys-PCL and mPEG-Ami-PCL) along with their inert counterpart (mPEG-Hex-PCL) were prepared and evaluated. These three copolymers were synthesized by conjugating mPEG and PCL with different linkers and characterized by proton nuclear magnetic resonance spectrometry, flourier transform infrared spectrometry and gel permeation chromatography. Nile red (NR) was loaded into the prepared nanoparticles as a model drug to study the in vitro drug release, cellular uptake amount and biodistribution. Dimethylcurcumin (DMC) was loaded into the prepared nanoparticles to study the in vitro antitumor effect. The results show that NR@mPEG-Cys-PCL and NR@mPEG-Ami-PCL nanoparticles exhibit glutathione (GSH)-triggered drug release and NR@mPEG-Ami-PCL nanoparticles display enhanced GSH-responsiveness as compared to NR@mPEG-Cys-PCL. Moreover, NR@mPEG-Ami-PCL nanoparticles possess enhanced cellular uptake amount as compared to NR@mPEG-Hex-PCL and NR@mPEG-Cys-PCL nanoparticles. DMC@mPEG-Ami-PCL nanoparticles possess the highest in vitro antitumor effect. In biodistribution study, both NR@mPEG-Cys-PCL and NR@mPEG-Ami-PCL nanoparticles show reduced organ distribution and similar tumor accumulation as compared to NR@mPEG-Hex-PCL nanoparticles. The mPEG-Ami-PCL nanoparticles developed in this work show great potential for anticancer drug delivery.
✓ 已验证
产品关联
DOI
高影响因子
Advanced Energy Materials
·
2024
·Vol. 14
·期 26
·2401197
·
IF 24.4
Mingli Xu,Chen Wu,Lang Ye,Yuanteng Zhang,Chenyi Zhang,Jieming Hu,Ran Tan,Dong Gu,Xuanze Wang,Olivier Fontaine,Chun Zhan,Lin Zhuang,Xinping Ai,Jiangfeng Qian
Degraded LiCoO 2 cathode from retired Li-ion batteries is urgently required to be recycled in a greener way for economic and environmental considerations. The coarse metallurgy technologies for Li/Co extraction with massive CO 2 emission and energy consumption cannot satisfy the requirements of carbon neutralization. Herein, it is proposed that direct regeneration of degraded LiCoO 2 cathode could be realized via 9-fluorenone-mediated Li supplementation and follow-up structural restoration. The 9-fluorenone-lithium reagent is elaborately selected to compensate for the missing Li + into lattice with targeted stoichiometry owing to its compatible redox potential of 1.95 V versus Li + /Li, which is located just between the reversible intercalation (3.8 V) and irreversible conversion (1.2 V) potentials of LiCoO 2 electrode. Then, thermal energy-driven structure reorganization enables Li/Co atoms to occupy the right sites, accomplishing desirable structure healing within a short annealing time of 4 h. The regenerated LiCoO 2 cathode exhibits comparable Li-storage capability to commercial LiCoO 2 , benefiting from the non-destructive direct regeneration technology. In addition, the regeneration route is regarded as environmentally (0.13 kg CO 2 kg −1 cell) and economically (10.07 $ kg −1 cell) superior to conventional recycling routes based on life-cycle analysis. The precise surgery on spent LiCoO 2 cathode provides a promising solution for the forthcoming retirement rush of Li-ion batteries.
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Zili Zhou,Yueying Pan,Lijie Zhu,He Li,Xinqi Liu,Lei Chen,Guozhen Wang,Qian Shen,Xiuying Liu,Wenping Ding
Soyasaponins (Ssa) have various biological benefits for the human body. However, they are one of the main factors contributing to the bitter taste of certain soybean-based foods. In this study, the case interaction between pectin and Ssa was investigated by us, as well as the impact of pectin addition on the bitter taste of Ssa. The findings revealed that the thermal stability of Ssa–pectin complexes was higher than that of Ssa–mucin complexes. Fluorescence spectroscopy and isothermal titration calorimetry revealed that pectin interferes with the binding of Ssa to mucin by interacting with Ssa, thereby disrupting the Ssa binding process. As the proportion of pectin increases, the comprehensive score also increases. This confirms that pectin can effectively reduce the bitterness and astringency of Ssa, signifying its importance in further exploration of the mechanism by which Ssa–pectin complexes influence the presentation of bitterness.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Haiyang Tian,Yunfeng Shi,Hongping Quan,Zhiyu Huang
The effect of CaCl 2 on the micromorphology of a self-thickening agent (TZXJ) was investigated. This study involved analyzing the compositional changes in acid solutions during acid–rock reactions. It combined dynamic light scattering and viscosity testing to confirm that the presence of CaCl 2 enhances the aggregation strength of the TZXJ network structure in acid solutions. This enhancement is macroscopically evident as an increase in viscosity. Incorporating 10 % CaCl 2 as a promoter into a fresh acid solution, the hydrodynamic radius (Rh) distribution of TZXJ in varying reaction stages was analyzed using dynamic light scattering experiments. The results indicated that the promoter CaCl 2 further strengthens the network association of the TZXJ acid solution, thereby improving its thickening capability. Investigating the viscosity of TZXJ aqueous solutions with varying concentrations of CaCl 2 revealed that TZXJ exhibits notable salt-thickening effects and can withstand saturated CaCl 2 solutions. The microstructural aggregation state and morphology of TZXJ in aqueous solutions were characterized at different CaCl 2 concentrations. Dynamic light scattering results demonstrated an increase in the Rh of TZXJ solutions with CaCl 2 concentrations. In particular, the mean Rh of a 0.8 % TZXJ solution increased from 150.8 nm to 3078.93 nm. Ultraviolet spectral analysis confirmed that the absorption intensity of the TZXJ solutions increased with CaCl 2 concentration, indicating a strengthened network aggregate structure in the TZXJ solutions. Microscopic observations using environmental scanning electron microscopy and transmission electron microscopy revealed that under the influence of calcium chloride, the microstructure of TZXJ transitions from a non-associated, linear carbon chain skeleton to a dense, aggregated network structure. The findings of this study offer theoretical insights for researching polymers resistant to extreme concentrations of CaCl 2 .
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Fang Xie,Xiaoqing Liu,Nian Liu,Xiaofang Feng,Zhijun He,Zia-ud Din,Shuiyuan Cheng,Yangchao Luo,Jie Cai
To develop a promising selenium supplement that overcomes the instability and poor water dispersibility of selenium nanoparticles (SeNPs), we synthesized a series of amphiphilic octenyl succinic anhydride starch (OSAS) through esterification. As the degree of substitution ( DS ) increased, the particle size of OSAS micelles and the critical micelle concentration (CMC) decreased. FTIR and XRD analysis confirmed the successful introduction of octenyl succinic anhydride groups onto starch. Subsequently, OSAS micelles were used as carriers to synthesize SeNPs via in situ chemical reduction, forming SeNPs-loaded self-assembled starch nano-micelles (OSAS-SeNPs). The OSAS-SeNPs exhibited spherical dispersion in water with an average diameter of 116.1 ± 2.3 nm, contributed to enhanced hydrophobic interactions. TEM images showed a core-shell structure with SeNPs as the core and OSAS as the shell. FTIR results indicated hydrogen bonding interactions between OSAS and SeNPs. Due to the negatively charged OSAS shell and hydrogen bonding (O H⋯Se), OSAS-SeNPs remained non-aggregated for one month at room temperature, demonstrating remarkable stability. This study suggests that using OSAS can address the synthesis and stability issues of SeNPs, making it a potential selenium supplement candidate for further evaluation as an anticancer agent.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Yiyang Liu,Ruolin Qin,Xiaoqiang Jia
Polycyclic Aromatic Hydrocarbons (PAHs) are highly toxic organic pollutants. Phenanthrene often serves as a model compound for studying PAHs biodegradation. In this work, we firstly engineered Escherichia coli M01 containing seven phenanthrene degradation genes and combined it with existing engineered strains E. coli M2 and M3 to form an artificial three-bacteria consortium, named M0123, which exhibited a degradation ratio of 64.66% for 100 mg/L of phenanthrene over 8 days. Subsequently, we constructed engineered Pseudomonas putida KTRL02 which could produce 928.49 mg/L rhamnolipids and integrated it with M0123, forming a four-bacteria consortium with an impressive 81.62% phenanthrene degradation ratio. Assessment of extracellular adenosine levels during the degradation process indicated high cellular energy demand in the four-bacteria consortium. Then, we introduced Bacillus subtilis RH33, a riboflavin-producing strain, as an energy-supplying bacterium, to create a five-bacteria consortium, which exhibited an 88.19% degradation ratio for phenanthrene. The NADH/NAD + ratio in the five-bacteria consortium during the degradation process was monitored, which was consistently higher than that of the four-bacteria consortium over the eight-day period, indicating a higher overall intracellular reduction capacity. Furthermore, the five-bacteria consortium displayed good tolerance to phenanthrene, even achieving a degradation ratio of 79.38% for 500 mg/L of phenanthrene. This study demonstrates that designing and constructing artificial consortia from the functional perspective and various angles can effectively enhance the degradation of phenanthrene after the addition of the energy-supplying bacterium. This study demonstrates that designing and constructing artificial labor-division consortia from the functional perspective and various angles can effectively enhance the degradation of phenanthrene.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaoyi Cheng,Ruiqi Peng,Mingxi Zou,Yan Chen,Wenkang Li,Zhongjiang Wang,Jing Xu,Zengwang Guo
The aim of this study was to construct soybean protein (SPI)-chitosan (CS)-liposomes (Lip) systems with different electrostatic strengths and to investigate the effects of the phase behavior and structural properties of SPI-CS on the structural homeostasis of Lip. It was demonstrated that the SPI-CS-Lips exhibited different aggregation behaviors due to the strength of the electrostatic interaction, which had an essential effect on the adsorption and stabilization of the Lips. The SPI-CS-Lips system was transparent at pH = 5 with a potential of 22.32 mV and an average particle size of 316.53 nm. At this time, the formation of more uniform polymers was also observed in the microscope. The stabilization of the SPI-CS-Lip system depended on the adsorption and fixed-point embedding of SPI-CS and Lip. From the membrane structural perspective, the lateral stacking efficiency of the phospholipid molecules was increased in the SPI-CS-Lip system at pH = 5, with the most excellent stiffening behavior exhibited by the Lip. This study was conducted to provide theoretical support for the construction and mechanism exploration of the novel Nano-Lip system.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）