SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Changying Cao,Quanhong Chang,Huijie Qiao,Runze Shao,Xin Guo,Guina Xiao,Wangzhou Shi,Lei Huang
The diffusion of generated H + ions in glucose sensing process is critical for the sensitivity of sensor. Although many glucose sensors assembled by two dimensional (2D) materials (MXene, graphene, MoS 2 et al.) have been extensively studied, the detailed analyses about the diffusion of H + ions are still rarely reported. Herein, hybrid Ti 3 C 2 -rGO film is fabricated by electrostatic self-assembly utilizing the 2D Ti 3 C 2 and graphene sheets to focus on the H + ion’s diffusion dynamic properties related to the sensing performance. Through controlling the graphene content, this hybrid Ti 3 C 2 -rGO film provide the effective H + ion diffusion channels. Based on the comprehensive analyses of cyclic voltammogram (CV) and electrochemical impedance spectroscopy (EIS), the corresponding localized conduction and enzymatic reaction at interfaces between the Ti 3 C 2 -rGO electrodes and glucose solution were investigated. According to the Warburg impedance in Nyquist plot from EIS, it is certified that the H + ion diffusion in low frequency range may play a significant role in the sensing mechanism of the glucose sensor. Moreover, the relationship derived from the interaction including interlayer spacing, H + ion diffusion and the sensing performance further reveal the H + ion diffusion as the dominant origin of the enhanced glucose sensitivity. This analysis will provide fundamental insight into the electrode structures assembled with 2D materials to optimize the sensor design in terms of ion transport dynamics.
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高影响因子
Drug Delivery and Translational Research
·
2021
·Vol. 12
·期 3
·603-614
·
IF 4.617
Shi Feng,Chen Lin,Wang Yaping,Liu Jing,Adu-Frimpong Michael,Ji Hao,Toreniyazov Elmurat,Wang Qilong,Yu Jiangnan,Xu Ximing
The objective of this study was to fabricate a novel drug delivery system using Soluplus® (polyvinyl caprolactam–polyvinyl acetate–polyethylene glycol graft copolymer) and glycyrrhizic acid to improve solubility, bioavailability, and anti-hyperuricemic activity of aloe emodin (AE). The AE-loaded mixed micelles (AE-M) were prepared by thin-film hydration method. The optimal AE-M contained small-sized (30.13 ± 1.34 nm) particles with high encapsulation efficiency (m/m, %) of 90.3 ± 1.08%. The release rate of AE increased in the micellar formulation than that of free AE in the four media (DDW, pH 7.0; phosphate buffer solution, pH 7.4; phosphate buffer solution, pH 6.8; and hydrochloric acid aqueous solution, pH 1.2). In comparison to free AE, the pharmacokinetic study of AE-M showed that its relative oral bioavailability increased by 3.09 times, indicating that mixed micelles may promote gastrointestinal absorption. More importantly, AE-M effectively reduced uric acid level by inhibiting xanthine oxidase (XOD) activity in model rats. The degree of ankle swelling, serum levels of interleukin (IL)-1, and IL-6-related inflammatory factors levels all decreased in the gouty arthritis model established via monosodium urate (MSU) crystals. Taken together, the AE-M demonstrated the potential to improve the bioavailability, anti-hyperuricemic activity, and anti-inflammation of AE. Graphical abstract
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高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Xiao-Yue Tang,Yi-Ming Liu,Xiao-Lin Bai,Hao Yuan,Yi-Kao Hu,Xiao-Ping Yu,Xun Liao
Dopamine (DA) is a critical biomarker for a variety of neurological diseases. Methods for simple and rapid DA detection are crucial for clinical diagnosis and treatments for those diseases. In this work, we developed a novel pretreatment-free method for dopamine detection using carbon dots as a turn-on fluorescent probe synthesized in situ . The aminosilane-functionalized carbon dots (SiCDs) were produced in a mild condensation reaction between N-[3-(Trimethoxysilyl)propyl]ethylenediamine (AEATMS) and dopamine, which were directly used for probing of dopamine. The prepared SiCDs exhibited green fluorescence with excitation/emission maximum at 380/495 nm, the intensity of which can be measured to quantify the DA present in the reaction mixture. The linear range of the assay was between 0.1 and 100 μM with a limit of detection (LOD) of 56.2 nM. The probe is of good selectivity and the recoveries of the developed method were in the range of 101.77–119.91% with RSDs within 3.67% in human serum sample tests. The SiCDs were also synthesized within MN9D cells under 37 °C and generated bright fluorescence, which can probe the DA’s distribution in the cells. The described method exhibit potential in DA detection and live-cell imaging for its feature of facility, inexpensiveness, and sensitivity.
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高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 14
·1672-1680
·
IF 2.896
Yalin Wu,Liping Lu,Zhihui Yu,Xiayan Wang
Based on the strategy of increasing the number of oxygen vacancies to improve the catalytic performance, we have developed a novel electrochemical sensor based on the multivalent metal oxides cerium dioxide and manganous oxide (Mn3O4/CeO2) for reliable determination of extracellular hydrogen peroxide (H2O2) released from living cells. The Mn3O4/CeO2 nanocomposite was characterized by high-resolution transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The electrochemical performance of the Mn3O4/CeO2 nanocomposite modified glassy carbon electrode (Mn3O4/CeO2/GCE) was investigated. Owing to the abundant oxygen vacancies and strong synergistic effect between the multivalent Ce and Mn, the sensor exhibited excellent catalytic activity and selectivity for the electrochemical detection of H2O2 with a low quantitation limit of 2 nM. Moreover, Mn3O4/CeO2/GCE exhibited excellent reproducibility, repeatability, and long-term storage stability. Because of these remarkable analytical advantages, the constructed sensor was able to determine H2O2 released from living cells with satisfactory results. The results showed that the Mn3O4/CeO2 sensor is a promising candidate for a nanoenzymatic H2O2 sensor with the possibility of applications in physiology and diagnosis.
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高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Xiaoqing Ma,Kang-lai Tang,Kang Lu,Chenke Zhang,Wenbing Shi,Wenxi Zhao
Designing metal sulfides with unique configurations and exploring their electrochemical activities for hydrogen peroxide (H2O2) and hydrazine (N2H4) is challenging and desirable for various fields. Herein, hollow microflower-like CuS@C hybrids were successfully assembled and further exploited as a versatile electrochemical sensing platform for H2O2 reduction and N2H4 oxidation, of which the elaborate strategies make the perfect formation of hollow architecture, providing considerable electrocatalytic sites and fast charge transfer rate, while the appropriate introduction polydopamine-derived carbon skeleton facilitates the electronic conductivity and boosts structural robustness, thus generating wide linear range (0.05–14 and 0.01–10 mM), low detection limit (0.22 μM and 0.07 μM), and a rather low overpotential (−0.15 and −0.05 V) toward H2O2 and N2H4, as well as good selectivity, excellent reproducibility, and admirable long-term stability. It should be highlighted that the operating potentials can compare favorably with those of some reported H2O2 and N2H4 sensors based on noble metals. In addition, good recoveries and acceptable relative standard deviations (RSDs) attained in serum and water samples fully verify the accuracy and anti-interference capability of our proposed sensor systems. These results not only elucidate an effective structural nanoengineering strategy for electroanalytical science but also advance the rational utilization of H2O2 and N2H4 in practicability.
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高影响因子
ANALYST
·
2021
·Vol. 146
·期 19
·5951-5961
·
IF 4.616
Hong Cui,Shuaishuai Cui,Siyuan Zhang,Qiuju Tian,Yunfeng Liu,Ping Zhang,Mingxiu Wang,Jialing Zhang,Xiangjun Li
The stability, repeatability and sensitivity of an electrochemical biosensor material are closely connected with the dispersibility of metal organic frameworks (MOFs) in aqueous media. Herein, a nanocomposite based on Cu–MOF/hemin, which is not only highly water-soluble but also simple and efficient in synthesis, was used for the construction of a non-enzymatic sensor to detect hydrogen peroxide (H2O2). The Cu–MOF/hemin was characterized via scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS)-mapping, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and thermal gravimetric analysis (TGA), which indicate that hemin and the Cu–MOF were successfully combined. As a H2O2 electrochemical biomimetic enzyme, the Cu–MOF/hemin exhibited excellent electrocatalytic performance, which was confirmed by the electrochemical experiments and chromogenic reactions, and the possible mechanism of the reactions has been deduced. The electrochemical sensor based on the biomimetic enzyme exhibited an extended linear detection range from 0.01–5.0 mM (R = 0.998), low detection limit of 4.14 μM, and high selectivity and stability under the optimized conditions. More importantly, the practical application ability of the sensor was verified by the test of H2O2 in human serum samples and it could be used for the real-time detection of H2O2 released from living cells with satisfactory results. Therefore, this novel nanocomposite has certain potential in preparing electrochemical sensing platforms for nonenzymatic biosensing and provides a new method for clinical diagnosis and real-time monitoring.
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高影响因子
ANALYST
·
2021
·Vol. 146
·期 17
·5403-5412
·
IF 4.616
Shiying Zhou,Xianfeng Wang,Liuyi Jiang,Human Sun,Danqun Huo,Changjun Hou
Two different electrochemical sensors (Hemin-G4/Au/GCE and Hemin-G4/Au/ITO) were developed and applied to explore the electrocatalytic capacity of H2O2 reduction. Due to the excellent catalytic activity of Hemin-G4 and high conductivity of gold nanoparticles, both electrodes show excellent electrochemical performances towards H2O2 with a low LOD (0.67 μM for Hemin-G4/Au/GCE and 0.65 μM for Hemin-G4/Au/ITO), rapid response (<4 s), and high selectivity and sensitivity (314.33 μA mM−1 cm−2 for Hemin-G4/Au/GCE and 322.22 μA mM−1 cm−2 for Hemin-G4/Au/ITO). The two electrodes allow sensitive capture of H2O2 produced by A549 cells. Compared with the conventional method of detection in cell suspensions, an ITO electrode with a large specific surface area and good biocompatibility can provide a promising platform for cell adhesion, so as to realize real-time and in situ detection of extracellular H2O2. The experimental results show that A549 cells can adhere to the surface of the Hemin-G4/Au/ITO electrode and grow well. This is benefitted from the three-dimensional structure of the Hemin-G4/Au hydrogel, which provides a suitable microenvironment for cell adhesion and growth. Furthermore, the in situ detection shows a faster response time than that of in-solution detection. This is because the H2O2 generated by the cells can be directly captured by the ITO electrode, which avoids diffusion from the solution to the electrode. These results indicate that the self-supporting hydrogel modified ITO electrode has great application prospects in basic biomedical research and continuous dynamic surveillance of diseases.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2021
·Vol. 168
·期 8
·087503
·
IF 4.316
Fangzhao Pu,Zhipeng Yu,Wenjing Lu,Chuncai Kong,Ruijun Lin,Xingyu Bai,Xiaojing Zhang,Jian Lv,Zhimao Yang
Fast, reliable, and low-cost glucose detection methods are of great importance for the control of diabetes. In this study, Cu2O combined with Cu nanoparticles anchored on reduced graphene oxide (RGO), was synthesized to function as the electrode in a non-enzymatic glucose sensor. The synergistic effect between Cu nanoparticles and Cu2O, together with the high conductivity of RGO enabled considerably lower resistivity with greater glucose oxidation activity than pure Cu2O. The RGO@Cu2O@Cu electrode exhibited a high sensitivity of 1.112 mA mM−1 cm−2 with a detection limit of 0.019 μM, and the linear range is up to 4 mM. Additionally, the RGO@Cu2O@Cu electrode displayed good anti-interference performance within human blood and high stability while operating for a long duration. Significantly, the glucose detection capability of the as-designed electrode was comparable to commercial sensors, representing a valuable design for future non-enzymatic glucose sensing.
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高影响因子
ELECTROANALYSIS
·
2021
·Vol. 33
·期 10
·2243-2251
·
IF 3.223
Kunpeng Miao,Long Yan,Ran Bi,Xiaoyan Ma
RGO/Au/Ni electrode was manufactured by a convenient, controllable, and environmental process, which was carried out by cyclic voltammetry (CV), and in this process, graphene-gold nanohybrid materials were simultaneously deposited on the nickel foam. Then the GOx was immobilized on the RGO/Au/Ni electrode by covalent bonding, and obtained the enzymatic biosensor. Scanning electron microscope (SEM) and Raman spectroscopy were adopted to confirm the microstructure of the fabricated RGO/Au/Ni electrode. Fourier transform infrared spectroscopy (FT-IR) was used to characterize the prepared enzymatic electrode. CV, chronoamperometry, and electrochemical impedance spectroscopy (EIS) were used to characterize the electrochemical performance of the fabricated enzymatic biosensor. It is found that AuNPs were well dispersed on the wrinkled RGO sheets, and the biosensor had a high sensitivity to glucose (32.83 μA ⋅ mM −1 ⋅ cm −2 ) with a wide linear range (0.15 26.15 mM), the strengths of anti-interference ability, good stability, and repeatability, etc.
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ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Xiaoning Ren,Ming Yang,Taotao Yang,Chao Xu,Yongqin Ye,Xiongni Wu,Xing Zheng,Bin Wang,Ying Wan,Zhiqiang Luo
Conductive polymer hydrogels (CPHs) hold significant promise in broad applications, such as bioelectronics and energy devices. Hitherto, the development of a facile and scalable synthesis method for CPHs with high electrical conductivity and biocompatibility has still been a challenge. Herein, we demonstrate highly conductive PPy–PEDOT:PSS hybrid hydrogels which are prepared by a simple solution-mixing method. This fabrication method involves the mixing of a pyrrole monomer with a PEDOT:PSS dispersion, followed by in situ chemical oxidative polymerization to form polypyrrole (PPy). The electrostatic interaction between negatively charged PSS and positively charged conjugated PPy facilitates the formation of PPy–PEDOT:PSS hybrid hydrogels. The conductivity of the PPy–PEDOT:PSS hybrid hydrogels is 867 S m–1. The PPy–PEDOT:PSS hybrid hydrogels show excellent biocompatibility. Moreover, the PPy–PEDOT:PSS hybrid hydrogels have a hierarchical porous structure which facilitates the 3D cell culture within the hydrogels. The PPy–PEDOT:PSS hybrid hydrogels exhibit excellent in situ biomolecular detection and real-time cell proliferation monitoring performance, indicating their potential as highly sensitive electrochemical biosensors for bioelectronics applications. Our strategy for the fabrication of CPHs with the electrostatic interaction between the negatively charged conductive polymer and positively charged conductive polymer would provide new opportunities for the design of highly conductive conjugated hydrogels for bioelectronics applications and energy devices.
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Wenyuan Zhao,Ying Ma,Jianshan Ye
Electrochemiluminescence (ECL) is widely used in bioanalysis due to its outstanding performance. In this work, we proposed a new ECL sensing strategy through the combination of molecular imprinting technology (MIT) and bipolar electrode (BPE). We use 3-aminophenyl boronic acid (APBA) as the monomer and dopamine (DA) as the template molecule to fabricate the MIP sensor through one-step electropolymerization. DA was encapsulated within the polymer film during the polymerization and subsequently removed to form a molecularly imprinted polymer (MIP) for DA recognition. The MIP modified electrode was applied as the sensing electrode, which could generate the oxidation current in the presence of DA, enabling the ECL signal change of reporting cell containing Ru(bpy) 3 2+ /TPrA in the BPECL system. Therefore, the rapid analysis of DA was achieved via this c-MIP-BPECL platform. The closed BPE simplifies sample pretreatment due to the relative independence of sensing and detecting, and the enrichment and precise identification of the analytes by MIP greatly improve the sensitivity, selectivity, and stability of the bipolar system. The established platform exhibits great sensing performance for DA with a linear range of 2 × 10 −7 –2.6 × 10 −4 M and 2.6–6.2 × 10 −4 M, respectively, with the low limit of detection (LOD) of 1 × 10 −7 M.
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高影响因子
ELECTROANALYSIS
·
2021
·Vol. 34
·期 2
·369-374
·
IF 3.223
Xiao Liu,Mei-Hao Xiang,Xinyue Zhang,Qin Li,Xiaoya Liu,Wenjing Zhang,Xia Qin,Fengli Qu
In this work, we reported the development of a nickel metal-organic framework nanosheet array on Ti-mesh (Ni-MOF/TM) as an enzyme-free electrochemical sensing platform for H 2 O 2 determination. The as-obtained sensor exhibited outstanding detection properties of H 2 O 2 , which might be gifted from the large specific surface area, abundant active sites of Ni-MOF nanoarrays. The sensor displayed a good linear range (0.8 μM–4.6×10 3 μM), a detection limit as low as 0.26 μM, a high sensitivity (307.5 μA mM −1 cm −2 ), and a rapid response. Moreover, this enzyme-free sensor is promising for point-of-care (POC) testing of H 2 O 2 in human serum attribute to the excellent performance of Ni-MOF and the simple preparation process of the sensor.
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