PHYTOMEDICINE
·
2025
·
IF 8.3
Xu Dong,Jianyin Miao,Ling Wu,Ziyu Kong,Zenghui Liu,Diyi Jia,Qingqing Zhai,Dan Zhang,Yan Xu
Background Diabetes patients face an elevated wound infection susceptibility and delayed healing processes. Currently, no existing literature has reported on the effect and mechanism of theaflavin-3, 3’-digallate nanoparticles (TFDG NPS) and TFDG NPS@hydrogels on diabetic wounds. Purpose Given that the treatment options for diabetic wound are limited, the aim of this study is to develop an innovative therapeutic approach to address diabetic wounds. Methods The TFDG NPS were prepared using ionic cross-linking, and they were then characterized. The biocompatibility of the TFDG NPS and TFDG NPS@hydrogel was assessed using a Cell Counting Kit-8 (CCK-8) assay and live/dead staining on HK-2 cells in vitro . Diabetic ICR mice were induced through intraperitoneal injection of streptozocin (STZ). They were then subjected to the creation of two full-thickness wounds on their dorsal areas. The effect and mechanism of the TFDG NPS and TFDG NPS@hydrogel on wound healing in diabetic mice were evaluated using a histological analysis, a western blot analysis, and molecular docking. Results The optimal TFDG NPS proportion was found to be TFDG:Gelatin (Gel):Chitosan (CS) = 2:1:1. Images photographed using a transmission electron microscope (TEM) revealed that the TFDG NPS appeared spherical, with a diameter of approximately 140 ± 20 nm. The favorable bio-compatibility of the TFDG NPS and TFDG NPS@hydrogel was confirmed using cell experiments. Animal studies demonstrated that both the TFDG NPS and TFDG NPS@hydrogel enhanced collagen fiber accumulation and new blood vessel density, reduced F4/80 infiltration, and upregulated the expression levels of TGF-β1, SMAD3, Collagen I, and α-SMA. The potential mechanism may involve activation of the TGF-β1/SMAD3 pathway, stimulating the secretion of Collagen I and α-SMA, and thereby facilitating wound closure in diabetic mice. The molecular docking results confirmed a high affinity between TFDG and TGF-β1/SMAD3. Conclusion TFDG NPS and TFDG NPS@hydrogel promoted wound closure in diabetic mice through the TFG-β1/SMAD3 pathway, thus exhibiting promising therapeutic potential for diabetic wound treatment.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Yihan Chen,Wanqing Wei,Jianhao Xu,Wei Song,Jian Wen,Guipeng Hu,Xiaomin Li,Cong Gao,Jing Wu
3-Hydroxy-3-methylbutyrate (HMB), an important dietary supplement, can enhance the quality of life for sedentary and elderly people. However, the catalytic efficiency of the pathway enzymes limited the biosynthesis of HMB. Here, we identified an efficient 4-hydroxyphenylpyruvate dioxygenase (4-HPPD) from Bacteroides barnesiae (BbHPPD) that converted l-Leucine (l-Leu) to HMB together with an efficient L-amino acid deaminase (L-AAD) in lab-preserved Proteus mirabilis (PmL-AADQ92A). The regioselective hydroxylation mechanism of the decarboxylated intermediate of 4-methyl-2-oxopentanoic acid (α-KIC) catalyzed by BbHPPD was elucidated for the first time. Structure-guided semirational design of BbHPPD generated a mutant M4. Compared to the wild type, the titer of mutant M4 increased by 254% when using α-KIC as a substrate. Finally, the engineered Escherichia coli 06 strain achieved the HMB titer of 11.6 g/L, a molar conversion of 65%, and a space-time yield of 0.64 g/(L·h) (the highest to date) in 18 h. This study lays the foundation for industrial-scale biosynthesis of HMB.
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JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 95
·期 12
·3149-3160
·
IF 2.75
Chuanlin Mou,Jiaxin Hu,Yingqing Zhan,Shuangjiang He,Yu-Hsuan Chiao,Shumei Zhao
BACKGROUND The large-scale oily wastewater from chemical industries and oil spills causes severe damage to ecosystems and affects human health. To date, plenty of superwetting membranes have been applied for efficient oil/water separation, but they still face many challenges when separating emulsified oily wastewater in harsh chemical conditions. RESULTS Herein, novel thermally and chemically stable super-hydrophilic/underwater super-oleophobic polymer nanofibrous composite membranes were fabricated by a facile and effective strategy. For this purpose, poly(arylene ether nitrile) (PEN) nanofibrous matting prepared from electrospinning was chosen as a supporting layer, which possessed excellent performance including anticorrosion, high thermal stability and tensile strength. Based on this, polydopamine (PDA) and 3-aminopropyltriethoxysilane (APTES) were easily and uniformly co-deposited onto the porous PEN supporting layer via a one-step immersion process. The resulting PEN composite membranes possessed stable super-hydrophilic/underwater super-oleophobic properties because of strong adhesion, abundant hydrophilic groups and the hierarchical micro-/nanostructure. Consequently, these membranes could separate various surfactant-stabilized emulsions under low driving pressure (0.04 MPa), with high permeation flux (6121 L m −2 h −1 ) and oil rejection ratio (>99.63%). Furthermore, the PEN nanofibrous membranes exhibited stable super-wetting and long-term separation performance under harsh operating conditions. CONCLUSION The super-hydrophilic/underwater super-oleophobic PEN membranes fabricated via a facile co-deposition strategy realized high separation efficiency, and excellent stability and antifouling property for emulsified oily wastewater under harsh operating environments, which indicated that they may have promising applications for the treatment of complicated oily wastewaters. © 2020 Society of Chemical Industry
研究领域:
环境科学
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材料科学
·
化学工程
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CHINESE CHEMICAL LETTERS
·
2014
·
IF 1.178
Hu-Yan Li,He Li,Bing-Jie Wang,Qun Gu,Zhi-Qiang Jiang,Xue-Dong Wu
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/chitin nanocrystals (CNC) composite scaffolds were synthesized by the salt leaching and thermally induced phase separation (TIPS) technique. The scaffolds have porous structures with macro-pores (100–300 μm in diameters) and micro-pores (10 μm). The surface characteristics of the scaffolds were characterized by X-ray photoelectron spectroscopy (XPS) and static water contact angle measurement, and the mechanical properties were investigated by a compression test. Human adipose-derived stem cells (hADSCs) were seeded onto the PHBV/CNC scaffolds and in vitro cell culture results showed that the composite scaffolds enhanced the hADSCs adhesion, which implies that the material may have potential application in tissue engineering.
研究领域:
生物材料学
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组织工程学
·
细胞生物学
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高影响因子
Cell Reports
·
2026
·Vol. 45
·期 4
·
IF 7.7
Yun Lv,Sheng Liu,Qihai Wang,Jing Zhu,Yingxiang Hou,Haiyan Xu,Deyan Zhu,Yu Liu,Jing Wu,Changxin Wu,Guijun Shang,Hongxiang Lou,Defen Lu,Huiqing Yuan,Deyu Zhu
The cyclic-oligonucleotide-based antiphage signaling system (CBASS) protects bacteria from phage infection. In Vibrio cholerae , phage infection activates CD-NTase DncV to produce 3′3′-cGAMP, which triggers phospholipase CapV to degrade phosphatidylethanolamine and phosphatidylglycerol, the major phospholipids in the inner-membranes, thereby inducing cell death. However, how 3′3′-cGAMP activates CapV was unclear. Here we present crystal structures of inactive Acinetobacter baumannii CapV in apo and 3′3′-cGAMP-bound forms, along with cryo-EM structures of activated CapV-3′3′-cGAMP complex, with or without substrate dioleoylphosphatidyl-ethanolamine (DOPE). Apo-CapV forms symmetric dimers in a “closed” state. 3′3′-cGAMP binding drives lateral polymerization of dimers into filament assembly, inducing an “open” state that exposes the active site and substrate-binding cleft. DOPE binding further shifts CapV to an “ajar” state, where a Y-shaped cleft positions DOPE for hydrolysis via a conserved Ser/Asp catalytic dyad. This 3′3′-cGAMP-induced filamentation mirrors activation mechanisms of TIR-STING, TIR-SAVED, and mammalian STING, revealing a conserved signaling pattern across immune systems.
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高影响因子
INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Jianlong Huang,Fengjiao Guo,Zhiyong Guo,Jianbang Chen,Bin Dai,Feng Yu
A series of ammonium-containing fluoroiodates, NH4IO2F2 and (NH4)3(IO2F2)3·H2O, with isolated [IO2F2] units have been fabricated by a fluorine-oxygen substitution strategy from NH4IO3. The two compounds crystallize in the orthorhombic system, but in different space groups, noncentrosymmetric Pca21 for NH4IO2F2 and centrosymmetric Pnma for (NH4)3(IO2F2)3·H2O, and show wide band gaps of 4.53 eV for (NH4IO2F2) and 4.55 eV for ((NH4)3(IO2F2)3·H2O). In addition, NH4IO2F2 exhibits a 1.2 × KDP second harmonic generation response, a short ultraviolet cutoff edge in iodates, and a good crystal growth habit. The crystal of NH4IO2F2 with a size of 11 × 5 × 2 mm3 was obtained by the aqueous solution method. The results enrich the structural diversity of iodate and supply a greater understanding of the design of new functional materials based on the fluoroiodates.
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高影响因子
PARASITOLOGY RESEARCH
·
2022
·Vol. 121
·期 2
·675-689
·
IF 2.383
Haseeb Muhammad,Huang Jianmei,Lakho Shakeel Ahmed,Yang Zhang,Hasan Muhammad Waqqas,Ehsan Muhammad,Aleem Muhammad Tahir,Memon Muhammad Ali,Ali Haider,Song Xiaokai,Yan Ruofeng,Xu Lixin,Li Xiangrui
Eimeria maxima (E. maxima) are an intracellular apicomplexan protozoan that causes intestinal coccidiosis in chickens. The purpose of this research was to develop a novel delivery approach for recombinant E. maxima (rEm) 14-3-3 antigen to elicit enhanced immunogenic protection using poly (D, L-lactide-co-glycolide) (PLGA) and chitosan (CS) nanoparticles (NPs) against E. maxima challenge. The morphologies of prepared antigen-loaded NPs (PLGA/CS-rEm14-3-3 NPs) were visualized by a scanning electron microscope. The rEm14-3-3 and PLGA/CS-rEm14-3-3 NPs–immunized chicken-induced changes of serum cytokines, IgY-antibody level, and T-lymphocyte subsets and protective efficacies against E. maxima challenge were evaluated. The results revealed that encapsulated rEm14-3-3 in PLGA and CS NPs presented spherical morphology with a smooth surface. The chickens immunized with only rEm14-3-3 and PLGA/CS-rEm14-3-3 NPs elicited a significant ( p <0.05) higher level of IFN-γ cytokine, stimulated the proportions of CD4 + /CD3 + , CD8 + /CD3 + T-cells, and provoked sera IgY-antibody immune response compared to control groups (PBS, pET-32a, PLGA, and CS). Whereas, PLGA-rEm14-3-3 NP–immunized chicken provoked a higher level of IFN- γ production and IgY-antibody response rather than CS-rEm14-3-3 and bare antigen, relatively. The animal experiment results ratified that PLGA-rEm14-3-3 NP–immunized chicken significantly alleviated the relative body weight gain (%), decreased lesion score, and enhanced oocyst decrease ratio compared to CS-rEm14-3-3 NPs and only rEm14-3-3. The anti-coccidial index of the chicken vaccinated with the PLGA-rEm14-3-3 NPs was (180.1) higher than that of the Cs-rEm14-3-3 NPs (167.4) and bare antigen (165.9). Collectively, our statistics approved that PLGA NPs might be an efficient antigen carrier system (Em14-3-3) to act as a nanosubunit vaccine that can improve protective efficacies in chicken against E. maxima challenge.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 139
·期 4
·51551
·
IF 3.125
You Wu,Yujuan Jin,Jian Huang,Huafeng Tian,Yunxuan Weng
Phenyl terminated hyperbranched polyesters (HBP-Bs) were synthesized based on Boltorn-type hydroxyl terminated HBPEs and characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance, gel permeation chromatography, and so forth. The prepared HBP-Bs were used to toughen poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) directly by melt blending. The thermal properties, rheological properties, mechanical properties, and microstructure of the PHBV/HBP-Bs blends were studied in detail. Comparing with pure PHBV, the crystallinity of the PHBV/0.5 phr HBP-Bs blends decreased by 14.10% (from 64.39% to 55.04%), the impact strength increased by 158.30% (from 7.41 kJ·m −2 to 19.14 kJ·m −2 ), and the elongation at break increased by 400.00% (from 0.91% to 4.55%). It can be seen from the scanning electron microscope that the fracture surface of the PHBV/HBP-Bs blends became rough with the increase of HBP-Bs, even with large filamentous structures and tiny holes, which further demonstrated that HBP-Bs acted as an excellent toughening effect on PHBV.
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MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Mengling Lin,Yingjie Guo,Zhiyong Liang,Xuesong Zhao,Jianan Chen,Yilin Wang
Biothiols, generally including glutathione (GSH), cysteine (CySH), and homocysteine (HcySH), are important biomarkers and closely related to many diseases. In this work, a simple method for the spectrophotometric determination of the three biothiols was developed. The proposed method was based on the fact that Fe 3+ could oxidize 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to oxidized TMB (ox-TMB) and induce a blue color solution corresponding to an absorption peak centered at 652 nm. However, the introduction of a biothiol compound could cause the reduction of ox-TMB which resulted in a blue color fading and a decrease of the absorbance. Based on this finding, we developed a method for the determination of biothiols with the help of UV–vis spectroscopy, and the influence of different factors including pH, temperature, and incubation time was studied when glutathione worked as an example. The detection limits for GSH, CySH, and HcySH were 1.04, 0.82, and 2.09 μM, respectively. More importantly, the suggested method was successfully applied for the determination of GSH in human serum samples with the recovery of 97%–112%. This method is simple, fast and without the use of complicated nanomaterials .
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ANALYST
·
2019
·Vol. 145
·期 3
·1025-1032
·
IF 4.019
Hui Xu,Zijie Feng,Yingxiang Du
Recently, ionic liquids have been widely used for chiral separation. However, several papers have reported the use of ionic liquids as the sole chiral selector. In this work, a novel capillary electrophoresis method using the ionic liquid 3-methyl-1-(3-sulfopropyl)-1H-imidazol-3-ium lactobionate (MSI-LA) as a chiral selector was developed for the enantioseparation of propranolol, metoprolol, bisoprolol, esmolol, atenolol, sotalol, terbutaline and clenbuterol. Method optimization was conducted and the optimized method (40 mM borax buffer, v/v 30% methanol, pH 8.5, 160 mM MSI-LA; +10 kV voltage; capillary temperature, 15 °C; 50 mbar/5 s injection; detection wavelength, 230 nm) was successful for the baseline separation of the eight basic drugs. The LA (lactobionic acid) system was compared. The possible mechanisms of enantioseparation were investigated using molecular modeling. Both the cation and anion of MSI-LA provided interactions between the chiral selector and analytes, and finally enhanced chiral recognition capability compared with LA.
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ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Guiyin Li,Bo Wang,Le Zhao,Xiaohang Shi,Guanxiong Wu,Wei Chen,Lixian Sun,Jintao Liang,Zhide Zhou
Glypican-3 (GPC3) protein is considered as an effective and specific biomarker for the accurate diagnosis of hepatocellular carcinoma (HCC), and there is a critical demand to construct strategies with shorter time, lower cost noise and higher sensitivity. In this study, a biosensor for label-free detection of GPC3 was prepared by modifying GPC3 aptamer (GPC3 Apt ) on the surface of Si-based light addressable potentiometric sensor (LAPS) chip modified by reduced graphene oxide/polyetherimide/gold nanoparticles (rGO/PEI/AuNPs). Among them, GPC3 Apt with a low equilibrium dissociation constant was used as a biorecognition molecule to recognize and capture GPC3. The rGO/PEI/AuNPs decorated on the LAPS surface could enlarge the surface area for GPC3 Apt immobilization and provide more active sites for GPC3 Apt to bind effectively with the target GPC3. When a bias voltage was provided to the LAPS system, the forming GPC3-GPC3 Apt complexs on the sensing interface of LAPS would cause the potential of the sensitive membrane surface to change and generate a corresponding offset response in photocurrent-voltage (I-V) curves. The potential shift and GPC3 concentration showed a good linear range from 0.1 to 100.0 μg/mL with a detection limit of 40.0 ng/mL (S/ N = 3) and a sensitivity of 1.33638 mV/μg/mL. In addition, the LAPS detection system also has good specificity and stability, which can be used for the detection of GPC3 in spiked serum samples.
研究领域:
生物传感器
·
癌症诊断
·
纳米技术
·
分析化学
·
生物医学工程
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高影响因子
STARCH-STARKE
·
2021
·
IF 2.741
Meidong Li,ZiMu Zhang,Ying Luo,Xiufang Huang,Kai Luo
Four new water-soluble polysaccharides has previously been isolated from Cardamine hupingshanensis . In this experiment, the structure of C. hupingshanensis polysaccharide NO. 3 (CHP-3) is analyzed and its in vitro anti-tumor activity is assessed. Through structural analysis, CHP-3 is found to have a molecular weight of 80.64 kDa, and be mainly composed of glucose, galactose, and L -arabinose; The backbone of CHP-3 is mainly composed of →5)-α-L-Araf-(1→ and →4)-α-D-Glc(p)-(1→), respectively. By studying its anti-tumor activity in vitro, it can be concluded that treatment with different concentrations of CHP-3 exerted diverse effects on A549 cells, with an obvious dose-dependent relationship. Moreover, after treatment with 12.8 mg mL −1 CHP-3, significant inhibitory effects are observed on the proliferation, migration, and invasion of A549 cells, indicating that it can be used as an anti-tumor agent for continued in-depth research.
研究领域:
药理学
·
肿瘤学
·
分子生物学
·
化学
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