Soft Matter
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2011
·Vol. 7
·期 14
·6451-6456
·
IF 4.457
Shengtong Sun,Peiyi Wu
Near infrared spectroscopy in combination with two-dimensional correlation spectroscopy (2Dcos) and perturbation correlation moving window (PCMW) technique is employed to illustrate the gelation microdynamic mechanism of hydrogelator N-octyl-D-gluconamide (8-GA), which can rapidly self-agglomerate into helical bilayer micellar fibers upon cooling from spherical micelles. Boltzmann fitting and PCMW easily determined the gelation temperature to be ca. 72 °C and the transition temperature range to be 70–75 °C. Moreover, band shifting and splitting phenomena can be observed for CH-related overtones, indicating the formation of much ordered and tight hydrophobic core from octyl tails. On the other hand, 2Dcos was used to discern the sequential orders during the gelation process and concluded that all the group motions have a continuous transfer from the octyl tail to the chiral carbohydrate head followed by the final immobilization of the solvent, which meanwhile, is actually a continuous dehydration process from the hydrophobic core to the outer hydrophilic chiral head. The driving force of the gelation process in microdynamics can only be the dehydration process of hydrophobic octyl chains, but with final helical superstructures being stabilized by amide-associated hydrogen bonding and the “chiral bilayer effect” of carbohydrate heads.
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RSC Advances
·
2017
·Vol. 7
·期 15
·8823-8831
·
IF 3.108
Tsogzolmaa Ganbold,Gerile Gerile,Hai Xiao,Huricha Baigude
RNA interference (RNAi) has shown great potential for clinical treatment of a variety of diseases. As an effective post-transcriptional regulation, RNAi induces degradation of specific target mRNA, leading to a decreased level of disease related gene product, which could be resistant to conventional small molecule therapeutics. Because of the relatively small size of short interfering RNA (siRNA) as well as it's robustness in the design of efficient 20–22 nucleotide sequences targeting any known gene, delivery of siRNA in vitro and in vivo has achieved tremendous success by the design and fabrication of various carrier materials including peptides, lipids, and polymers. While siRNA delivery efficiency of these materials proved to be appreciable, their tissue specificity, biocompatibility and cytotoxicity still need much improvement. We report a novel siRNA delivery material designed by optimization of our previously reported peptidomimetic based lipoplex. The novel lipoplex, which bears a D-amino acid based dipeptide head group, possesses an ideal balance of increased stability in a tissue environment, and enhanced efficiency of tissue specific delivery as well as minimized cytotoxicity. The novel lipoplex carrying siRNA efficiently targeted mouse liver, initiated RNAi and knocked down the apoB dose dependently, which is confirmed by both RT-qPCR and Western blotting. Moreover, the novel lipoplex does not induce any apparent cytotoxicity, as confirmed by the measurement of liver enzyme levels in serum.
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Cell Death Discovery
·
2025
·Vol. 11
·期 1
·1-15
·
IF 7
Cheng Mengting,Wu Wentong,Li Qing,Tao Xinyu,Jiang Feng,Li Jinjin,Shen Nonger,Wang Fei,Luo Peihua,He Qiaojun,huang Ping,Xu Zhifei,Zhang Yiwen
Sotorasib, the inaugural targeted inhibitor sanctioned for the management of patients afflicted with locally advanced or metastatic non-small cell lung cancer presenting the KRAS G12C mutation, has encountered clinical application constraints due to its potential for cardiac injury as evidenced by safety trials. This investigation has elucidated that the heightened expression of neuraminidase-1 (NEU1) constitutes the principal etiology of cardiac damage induced by sotorasib. Mechanistically, sotorasib treatment inhibited the ubiquitinated degradation of NEU1, leading to its elevated expression, which induced downstream AKT signaling pathway inhibition and mitochondrial dysfunction leading to cardiomyocyte apoptosis. Meanwhile, in vivo and in vitro studies showed that D-pantothenic acid (D-PAC) alleviated sotorasib-induced cardiac damage by promoting NEU1 degradation. In conclusion, this study revealed that NEU1 is a key protein in sotorasib cardiotoxicity and that reducing the level of this protein is a critical strategy for the clinical treatment of sotorasib-induced cardiac injury. Schematic representation of a mechanism.
研究领域:
肿瘤学
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心脏病学
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分子生物学
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药理学
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Scientific Reports
·
2016
·Vol. 6
·期 1
·1-10
·
IF 5.228
Liang Chaoning,Zhang Yi,Jia Yan,Wenzhao Wang,Li Youhai,Lu Shikun,Jin Jian-Ming,Tang Shuang-Yan
Glycodiversification broadens the scope of natural product-derived drug discovery. The acceptor substrate promiscuity of glucosyltransferase-D (GTF-D), a carbohydrate-processing enzyme from Streptococcus mutans , was expanded by protein engineering. Mutants in a site-saturation mutagenesis library were screened on the fluorescent substrate 4-methylumbelliferone to identify derivatives with improved transglycosylation efficiency. In comparison to the wild-type GTF-D enzyme, mutant M4 exhibited increased transglycosylation capabilities on flavonoid substrates including catechin, genistein, daidzein and silybin, using the glucosyl donor sucrose. This study demonstrated the feasibility of developing natural product glycosyltransferases by engineering transglycosidases that use donor substrates cheaper than NDP-sugars, and gave rise to a series of α-glucosylated natural products that are novel to the natural product reservoir. The solubility of the α-glucoside of genistein and the anti-oxidant capability of the α-glucoside of catechin were also studied.
研究领域:
生物化学
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药物开发
·
合成生物学
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APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Xiaojie Liu,Yong Zhao,Yahui Du,Quanliang Wang,Xin Jing,Liangmin Yu,Mingliang Sun
Conjugated donor (D)–acceptor (A) polymer heterojunctions have developed as attractive photocatalysts. However, it is a great difficulty in precisely keeping the batch-to-batch reproducibility of polymers and controlling the related photocatalytic activity . Herein, three ladder-like small molecules BTx (BT2F, BTORCAE, and BTORIC2Cl) are synthesized via molecular engineering and the corresponding BTx/g-C 3 N 4 heterojunctions are developed as photocatalysts for sterilization and contaminant degradation. It is demonstrated that employing another A unit into the D–A–D backbones of the small molecule could promote its intramolecular charge transfer and also improve the intermolecular charge transfer. Notably, BTORIC2Cl employing 2-(5,6-Dichloro-3-oxo-2,3 dihydro-1H-inden-1-ylidene)malononitrile as A 1 unit in its symmetric A 1 –D–A 2 –D–A 1 structure displays near-infrared absorption and extends the absorption edge of BTORIC2Cl/g-C 3 N 4 to 850 nm. With the synergy of efficient intermolecular charge transfer, BTORIC2Cl/g-C 3 N 4 contributes the champion photocatalytic activity of 98.9 % and 99.6 % antibacterial rates for Staphylococcus aureus and Escherichia coli . Moreover, the highest degradation reaction rate constant for Rhodamine B obtained by BTORIC2Cl/g-C 3 N 4 is 5.7 times that of g-C 3 N 4 . This study proposes new ideas of fabricating small molecules/g-C 3 N 4 OSHJs as photocatalysts via rational molecular engineering.
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JOURNAL OF CHEMICAL THERMODYNAMICS
·
2016
·
IF 2.196
Dandan Han,Yumin Liu,Xiaona Li,Teng Zhang,Zhenfang Li,Shichao Du,Mingxia Guo,Shijie Xu,Bo Yu,Baohong Hou,Junbo Gong
The solubility of calcium d -pantothenate in (water + methanol) mixtures and the effect of β -alanine on the solubility of calcium d -pantothenate in a constant mole ratio of methanol and water was measured from 263.15 K to 303.15 K by the HPLC analysis method ( P = 0.1 MPa). The solubility of calcium d -pantothenate increased with increasing temperature in all the mixtures at constant composition. Besides, it also increased with mole fraction of water in (water + methanol) mixtures at a constant temperature. Additionally, the solubility of calcium d -pantothenate was enhanced by β -alanine at a given temperature. The experimental solubility in (water + methanol) mixtures was correlated by the modified Apelblat equation, the ternary NRTL equation and Solubility-Polarity Model. The Apelblat equation, the quaternary NRTL equation and the Cohn equation were adopted to correlate the experimental solubility in the (water + methanol + β -alanine) mixtures. Worthwhile, the quaternary NRTL equation was first applied to correlating the quaternary solid-liquid equilibrium. It turned out that all the selected models give good performance on solubility prediction.
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CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Luyao Huang,Yuntian Lou,Dawei Zhang,Lingwei Ma,Hongchang Qian,Yuting Hu,Pengfei Ju,Dake Xu,Xiaogang Li
In this study, antibacterial surfaces with a “disperse-then-kill” synergy were prepared by embedding d -cysteine ( d -cys) functionalised silver nanoparticles (AgNPs) onto polydopamine-coated stainless steel. The as-prepared surface exhibited excellent antibacterial performance against the gram-negative Escherichia coli and Pseudomonas aeruginosa PAO1 and gram-positive Staphylococcus aureus . This component of d -cys could inhibit the formation of mature biofilms and maintain bacteria in dispersed and planktonic states, which further facilitates the bacterial-killing action enabled by the AgNPs. The cooperation of these two components presents a new strategy for developing antibacterial surfaces with a stronger and more durable performance. Furthermore, the as-prepared surface did not exhibit an apparent cytotoxicity based on the results of real-time cell analyzer (RTCA) and a cell viability kit-8 assay (CCK-8 assay). Given these results, the proposed antibacterial strategy demonstrated a potential to inhibit bacterial adhesion and biofilm formation on surgical devices and medical implants.
研究领域:
材料科学
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生物材料
·
生物医学工程
·
微生物学
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Fuping Wang,Xinyi Peng,Zhengpeng Zhong,Mingchen He,Qinyan Lu,Yifan Li,Huaiyang Liu,Wang Li,Guobao Chen,Bo Zhou,Zhongmin Chen
A non-isotopic methanol-releasing probe detects HCC early via breath. • Selectively cleaved by β-galactosidase in HCC for tumor-specific signal. • A low-cost dual-sensor array enables real-time exhaled methanol detection. • Distinguishes early-stage tumor-bearing mice from controls (< 2 mm). • Offers a portable, affordable screening tool for primary healthcare. Early detection is crucial for improving therapeutic outcomes in hepatocellular carcinoma (HCC), yet the widespread implementation of low-cost, accessible screening for high-risk populations remains a significant challenge. To address this issue, we investigated the β-galactosidase–methanol signaling axis using a hydrophilic, cell-membrane-impermeable probe, methyl-β-D-galactopyranoside (MGP). This probe is selectively hydrolyzed by β-galactosidase, which is overexpressed in the HCC microenvironment, releasing methanol—an easily volatile, non-isotopic, and metabolically inert volatile organic compounds (VOCs). Methanol levels in exhaled breath were quantified using a custom-designed sensor array. Through in vitro enzymatic assays, cellular studies, and animal experiments, we demonstrated that methanol concentrations in tumor-bearing mice (with tumor diameters <2 mm) were significantly elevated compared to healthy controls, and these levels positively correlated with tumor volume. Our findings highlight the potential of Me-β-Gal in addressing critical barriers to early HCC detection. With further validation, this approach could potentially improve clinical management for high-risk populations, including patients with liver cirrhosis.
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JOURNAL OF CONTROLLED RELEASE
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2026
·
IF 12.4
Beibei Li,Qingchao Wang,Xiaoxi Wang,Peixin Chen,Xinyu Zi,Yanping Ma,Qiaoya Xuan,Ying Cheng,Shihan Zhang,Hanke Feng,Wenjie Zhai,Yahong Wu,Yumiao Zhao,Xiaowen Zhou,Xiaoshuang Niu,Wenshan Zhao
Effective cancer immunotherapy is often hindered by V-domain Ig suppressor of T cell activation (VISTA) signaling and myeloid-derived suppressor cells (MDSCs) within tumor microenvironment (TME). Here, we report D-peptide-nanodrug conjugates that synergize VISTA blockade and innate activation to overcome these constraints. We developed Reorpn7, a proteolytically stable D-peptide VISTA inhibitor with high binding affinity and in vitro serum stability. This inhibitor was co-loaded with a toll-like receptor 7/8 (TLR7/8) agonist (R848) into an matrix metalloproteinase-2 (MMP-2) responsive poly lactic-co-glycolic acid (PLGA) nanoplatform (r7PL-PLGA@R848). Upon enrichment in the TME, MMP-2 responsive cleavage enables the precise release of the VISTA-targeting peptide Reorpn7 and the TLR7/8 agonist R848, thereby achieving synergistic therapeutic effect of VISTA blockade and innate immune activation. In MC38 tumor models, this nanosystem significantly increased intratumoral CD8 + T cell infiltration and achieved robust antitumor efficacy without obvious systemic toxicity. Mechanistically, the platform reshaped the TME from an inhibitory milieu into an immunosupportive one by coordinating checkpoint blockade, myeloid reprogramming, and T cell recruitment. This stimuli-responsive paradigm offers a translatable strategy to overcome immune checkpoint blockade resistance in challenging malignancies.
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Surfaces and Interfaces
·
2026
·
IF 6
Qingpo Yang,Shangkang Xie,Zixiang Xing,Jie Liu,Yufeng Shen,Ling Huang,Rubo Fang,Feng Feng,Chunshan Lu,Qingtao Wang,Chong Yao,Qunfeng Zhang,Xiaonian Li
The shielding effect effectively suppresses the chemical adsorption between platinum and sulfur species and hinders the formation of Pt-S bonds. • Electrons transfer from Pt to the carbon encapsulation layer to construct metal-carbon interfacial structures, which endows the catalyst with superior hydrogenation activity in sulfur-containing environments. • Carbon-encapsulated structure enhances the structural stability and sulfur resistance of the catalyst. • Experimental characterizations combined with DFT calculations elucidate the underlying reaction mechanism. The presence of sulfur-containing impurities in industrial feedstocks leads to the rapid deactivation of metal-based catalysts, which originates from the specific recognition between the d orbitals of metallic active sites and the p orbitals of sulfur. In this work, a carbon-encapsulation catalyst strategy was employed to shield this specific d–p orbital recognition effect. The Pt-base catalyst showed a sharp 92.28% loss in activity after sulfur poisoning, whereas the carbon-encapsulated catalyst maintained high efficiency and stability, sustaining a reaction rate of 83.41 min –1 even at high mercaptan concentrations (7945 ppm). Moreover, it exhibited excellent reusability with negligible deactivation after five cycles. Characterization and density functional theory (DFT) calculations revealed that the carbon shell serves as “armor” for Pt active sites, physically isolating them from sulfur impurities and suppressing the hybridization between Pt nanoparticles (Pt NPs) and the S 2p orbitals. Meanwhile, Pt nanoparticles induce electron transfer to the carbon layer, converting it into a conductive “metallic carbon” that enhances hydrogenation activity. This work provides new insights into the anti-sulfur poisoning mechanism of catalytic hydrogenation. Download: Download high-res image (255KB) Download: Download full-size image
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Jing-Jing Liu,Si-Dian Miao,Zhan-Yi Wang,Ya-Han Zhu,Bin-Bin Jiang,Shuang-Shuang Long,Shu-Qin Gao,Ying-Wu Lin
The anti-glycation effects and mechanisms of eight quinone-related compounds, primarily sourced from medicinal and edible plants, were evaluated in this study. In vitro glycation experiments were conducted to study the structural perturbation induced by d -ribose on human myoglobin (HMb) in the presence of the tested compounds. Spectroscopic and electrophoretic analyses indicated that among the four benzoquinone-related substances, thymoquinone and its structurally closest analogue 2,5-dimethyl-1,4-benzoquinone, exhibited significant inhibitory effects on the d -ribose-induced glycation, thereby maintaining protein structural stability, reducing advanced glycation end products (AGEs) formation, and attenuating the covalent protein modification by d -ribose. In contrast, 1,4-benzoquinone and hydroquinone (a reduction product of 1,4-benzoquinone) promoted HMb denaturation through the Michael addition mechanism. Of the two naphthoquinones, juglone and plumbagin, showed anti-glycation effects, whereas the anthraquinones emodin and chrysophanol showed no significant effects. Nano-HPLC-MS/MS analysis further showed that thymoquinone was more effective in preventing glycation than juglone and plumbagin. This study investigates the mechanisms underlying glycation inhibition by quinones, revealing that the presence of dual β -substituted carbons on the benzoquinone ring is critical for stabilizing protein conformations and suppressing glycation. It provides a preliminary mechanistic basis and identifies candidate molecules for potential applications of quinones in retarding glycation, such as in managing diabetic complications or inhibiting glycation reactions during food processing.
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Applied Sciences-Basel
·
2025
·Vol. 15
·期 13
·7318
·
IF 2.5
Jiawei Cui,Yan Li,Ming Yan
D-allulose is a rare sugar with promising applications in food and health industries, owing to its low caloric value and multiple health benefits. In this study, we systematically investigated a thermostable D-allulose 3-epimerase (TcDAEase) fromThermogemmatispora carboxidivoransfor food-compatible continuous production. The enzyme exhibited remarkable thermostability, with over 70% activity retained at 80 °C, and showed broad pH tolerance across the range of 8.0 to 13.0. Notably, TcDAEase exhibited high catalytic activity toward D-allulose and D-fructose even without the addition of metal ions. Moreover, food-grade Mg2+was identified as enhancing enzyme activity by 14.3%, thus ensuring compliance with Generally Recognized as Safe (GRAS) standards for food applications. To improve industrial applicability, the enzyme was immobilized using a chitosan-diatomaceous earth (DE) matrix via three-step adsorption–crosslinking–embedding strategy. The immobilized TcDAEase achieved 48.7% ± 2.4% activity recovery and retained 90.3% ± 1.5% activity over seven reaction cycles. Furthermore, continuous production of D-allulose was realized in a packed-bed reactor, operating stably at 60 °C, pH 8.0 and 5 mM Mg2+for 150 days, producing 756 kg of D-allulose with a conversion yield exceeding 89.7% of the theoretical maximum. Overall, this study provides a feasible strategy for the continuous and efficient production of high-value-added D-allulose in the food industry.
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危险品化学品经营许可证（带存储）