EUROPEAN JOURNAL OF PHARMACOLOGY
·
2020
·
IF 3.263
Cui Zhai,Wei Feng,Wenhua Shi,Jian Wang,Qianqian Zhang,Xin Yan,Qingting Wang,Shaojun Li,Lu Liu,Yilin Pan,Yanting Zhu,Limin Chai,Cong Li,Pengtao Liu,Yuqian Chen,Manxiang Li
It has been shown that sphingosine-1-phosphate (S1P) is elevated in patients with pulmonary arterial hypertension (PAH) and promotes the proliferation of pulmonary artery smooth muscle cells (PASMCs). Meanwhile, S1P has been found to induce the activation of autophagy in several types of human diseases including cancers. However, it is still unclear whether activation of autophagy mediates S1P-induced PASMCs proliferation, and detailed mechanisms responsible for these processes are indefinite. The aims of this study are to address these issues. S1P dose- and time-dependently reduced the expression of E-cadherin/CDH1 and stimulated PASMCs proliferation; this was accompanied with the elevation of TNF receptor-associated factor 2 (TRAF2), up-regulation and ubiquitination of BECN1 and the activation of autophagy. Prior silencing TRAF2 or BECN1 using siRNA or pre-incubation of cells with autophagy inhibitor chloroquine phosphate (CQ) suppressed S1P-induced autophagy activation and subsequent CDH1 degradation and further PASMCs proliferation. Taken together, our study indicates that S1P promotes the activation of autophagy by accelerating TRAF2-mediated BECN1 up-regulation and ubiquitination, which in turn results in CDH1 reduction and contributes to PASMCs proliferation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Chuang Wang,Yiqi Liao,Hou-Yong Yu,Yanjuan Dong,Juming Yao
Fiber-based wearable electronic textiles have broad applications, but non-degradable substrates may contribute to electronic waste. The application of cellulose-based composite fibers as e-textiles is hindered by the lack of fast and effective preparation methods. Here, we fabricated polyaniline (PANI)/cellulose fibers (PC) with a unique skin-core structure through a wet-spinning homogeneous blended system. The conductive network formation was enabled at a mere 1 wt% PANI. Notably, PC15 (15 wt% PANI) shows higher electrical conductivity of 21.50 mS cm −1 . Further, PC15 exhibits excellent ammonia sensing performance with a sensitivity of 2.49 %/ppm and a low limit of detection (LOD) of 0.6 ppm. Cellulose-based composite fibers in this work demonstrate good gas sensing and anti-static properties as potential devices for smart e-textiles.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2023
·Vol. 11
·期 32
·17112-17124
·
IF 11.9
Kai Han,Dadong Zhang,Wenbo Zhuang,Yanfen Wan,Peng Yang
Wearable sweat biosensors, as the only noninvasive and measurable body-fluid sensors, serve as a very important means to monitor an individual’s health status and more dynamic molecular-level information. The pursuit of wearable sweat biosensors includes not only detecting sweat components, but also integrating multiple functions, such as sweat-based health monitoring, near-field transmission and being self-powered. In this paper, we firstly bring forth a new idea of integrating sweat monitoring and dual electrical generation (hydrovoltaic and moisture electricity generation) for a multifunctional flexible device as a sweat-based health monitoring electronic (E)-skin. Herein, besides sweat composition analysis, a hydrovoltaic electricity generator is employed to detect the amount of sweat for the first time, and a dual electricity generator is used for self-supply of energy and skin-surface temperature measurement. Synergistic tandem nanogenerators are constructed using biomass carbon materials, and the power generation voltage can reach about 500 mV and is relatively stable. Our sweat E-skin (SE-skin) has five main functional areas, realizing real-time monitoring of sweat volume, sweat composition analysis‚ skin-surface temperature measurement and power-supply functions. This new integrated design not only enables real-time monitoring of indicators of health in sweat, but also provides a new idea for future intelligent wearables for human beings.
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2023
·
IF 4.1
Yuzhu Li,Kangzhuo Yang,Zhanglan He,Zhipeng Liu,Jialing Lu,Dong Zhao,Jia Zheng,Michael C. Qian
Electronic nose (E-nose) technology is frequently attempted to simulate the human olfactory system to recognize complex odors. Metal oxide semiconductors (MOSs) are E-noses’ most popular sensor materials. However, these sensor responses to different scents were poorly understood. This study investigated the characteristic responses of sensors to volatile compounds in a MOS-based E-nose platform, using baijiu as an evaluation system. The results showed that the sensor array had distinctive responses for different volatile compounds, and the response intensities varied depending on the sensors and the volatile compounds. Some sensors had dose–response relationships in a specific concentration range. Among all the volatiles investigated in this study, fatty acid esters had the greatest contribution to the overall sensor response of baijiu. Different aroma types of Chinese baijiu and different brands of strong aroma-type baijiu were successfully classified using the E-nose. This study provided an understanding of detailed MOS sensor response with volatile compounds, which could be further applied to improve the E-nose technology and its practical application in food and beverages.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Weibin Ma,Luling Zhao,Wenhong Zhao,Yanli Xie
Fungal contamination imposes threats to agriculture and food production and human health. A method to safely and effectively restrict fungal contamination is still needed. Here, we report the effect and mode of action of (E)-2-hexenal, one of the green leaf volatiles (GLVs), on the spore germination of Aspergillus flavus, which can contaminate a variety of crops. The EC50 value, minimum inhibitory concentration (MIC), and minimum fungicidal concentration (MFC) of (E)-2-hexenal were 0.26, 1.0, and 4.0 μL/mL, respectively. As observed by scanning electron microscopy (SEM), the surface morphology of A. flavus spores did not change after treatment with the MIC of (E)-2-hexenal, but the spores were shrunken and depressed upon treatment with the MFC of (E)-2-hexenal. The MIC and MFC of (E)-2-hexenal induced evident phosphatidylserine (PS) externalization of A. flavus spores as detected by double staining with Annexin V-FITC and propidium iodide, indicating that early apoptosis was potentially induced. Furthermore, sublethal doses of (E)-2-hexenal disturbed pyruvate metabolism and reduced the intracellular soluble protein content of A. flavus spores during the early stage of germination, and MIC treatment decreased acetyl-CoA and ATP contents by 65.7 ± 3.7% and 53.9 ± 4.0% (P < 0.05), respectively. Additionally, the activity of mitochondrial dehydrogenases was dramatically inhibited by 23.8 ± 2.2% (P < 0.05) at the MIC of (E)-2-hexenal. Therefore, the disruption of mitochondrial energy metabolism and the induction of early apoptosis are involved in the mechanism of action of (E)-2-hexenal against A. flavus spore germination.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROCERAMICS
·
2019
·Vol. 43
·期 1
·96-105
·
IF 1.966
Wang Ziyang,Chen Peng,Shi Wenjing,Li Yang,Li Dequan,Zhang Leiyang,Yan Yan,Wang Yifei,Peng He,Li Quan,Liu Gang
In the current investigation, the CuO modified BaTiO 3 ceramics were prepared through the conventional electroceramic processing. XRD, XPS, and SEM have been employed to characterize the phases, valences of ions and the microstructures. The sintering behaviors of the prepared samples were performed using a dilatometer, and ceramics could be fabricated at lower temperature. The electric field and temperature dependent energy storage performance have been studied. The introducing of CuO is very effective to improve the energy storage density and the efficiency due to the formed double P-E loops. 0.5 wt.% CuO modified ceramic exhibited an energy density of 0.52 J/cm 3 , which is nearly twice of pure BaTiO 3 ceramic. The J-E loops of CuO modified samples indicated double domain switches, consistent with the double P-E loops.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Shulong Jiang,Mengying Li,Ying Hu,Zhenhai Zhang,Huixia Lv
A polymer of Galactosamine-hyaluronic acid-Vitamin E succinate (Gal-HA-VES) was designed to prepare multifunctional self-assembled micelles for delivery of Norcantharidin (NCTD) to Hepatic carcinoma. NCTD/Gal-HA-VES showed higher cytotoxicity toward CD44-overexpressing MCF-7 cells, MCF-7/Adr cells and ASGP-R overexpressing HepG2 cells, consistent with the enhanced cellular uptake in the selected cell models, indicating Gal-HA-VES micelles were taken up in MCF-7 and HepG2 cells by CD44 and ASGPR receptor mediated endocytosis, respectively. Moreover, the accumulation of Rhodamine 123 demonstrated that Gal-HA-VES has the same action of TPGS as a P -glycoprotein inhibitor blocked drug efflux-related MDR mechanism in resistant MCF-7/Adr cells. The Cell apoptosis assays indicated that NCTD/Gal-HA-VES were more effective in triggering apoptosis, compared with free NCTD or NCTD/HA-VES groups. In vivo study demonstrated that NCTD/Gal-HA-VES group exhibited enhanced tumor targeting and antitumor activity with lower systemic toxicity. Hence NCTD/Gal-HA-VES micelles system can achieve significant tumor targeting and effective treatment of hepatic carcinoma.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2018
·Vol. 6
·期 12
·3397-3409
·
IF 5.831
Jing-Bo Hu,Di Liu,Jing Qi,Kong-jun Lu,Fei-yang Jin,Xiao-Ying Ying,Jian You,Yong-Zhong Du
Based on the overproduction of matrix metalloproteinase-2 (MMP-2) in renal tissue during acute kidney injury (AKI) occurrence, we developed a MMP-2 enzyme-triggered polymeric prodrug with sialic acid (SA) as the targeting group to the inflamed vascular endothelial cells for enhanced therapeutic outcomes. An MMP-2-responsive peptide, PVGLIG, was used to endow the polymeric prodrug with the ability to rapidly release the anti-inflammatory drug, curcumin (CUR), after the targeted site is reached and to improve the drug concentration in the target tissue. The sialic acid–dextran–PVGLIG–curcumin (SA-DEX-PVGLIG-CUR) polymeric prodrug was successfully synthesized via multi-step chemical reactions and characterized by 1H NMR. The water solubility of CUR was significantly increased in the polymeric prodrug and was approximately 23-fold higher than that of free CUR. The in vitro drug release results showed that the release rate of SA-DEX-PVGLIG-CUR was significantly enhanced compared to that of SA-DEX-CUR in a dissolving medium containing the MMP-2 enzyme, suggesting that SA-DEX-PVGLIG-CUR had rapid drug release characteristics in an inflammatory environment. A cellular uptake test confirmed that SA-DEX-PVGLIG-CUR was effectively internalized by inflamed vascular endothelial cells in comparison with that by normal cells, and the mechanism was associated with the specific interaction between SA and E-selectin receptors specifically expressed on inflamed vascular endothelial cells. Bio-distribution results further demonstrated the rapid and increased renal accumulation of SA-DEX-PVGLIG-CUR in AKI mice. Benefiting from the rapid drug release in renal tissue, SA-DEX-PVGLIG-CUR effectively ameliorated the pathological progression of AKI compared with free CUR and SA-DEX-CUR, as reflected by the improved renal functions, histopathological changes, pro-inflammatory cytokine production, oxidative stress and expression of apoptosis related proteins. Altogether, this study provided a new therapeutic strategy for the treatment of AKI.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Solar RRL
·
2017
·Vol. 1
·期 10
·1700118
·
IF 0
Junjie Ma,Xiaolu Zheng,Hongwei Lei,Weijun Ke,Cong Chen,Zhiliang Chen,Guang Yang,Guojia Fang
In recent years, photovoltaic properties of hybrid perovskite solar cells (PSCs) have led to impressive improvement. Yet, there is still remain a challenge for large scale application and commercialization. Some of the reasons are the instability of the perovskite film and the lack of large-scale and low-cost manufacture techniques for electron transport layer (ETL). Tin oxide (SnO 2 ) has emerged as a promising candidate for ETL, however, systematic exploration of its commercialized application in PSCs is still missing. Here, SnO 2 ETL is prepared through an electron beam evaporation technology under low-temperature process to realize large-scale, low-cost, and uniform manufacture. Hundreds of SnO 2 ETL substrates can be fabricated at one time, which deserves industrial deployment. As a result, PSCs based on e-beam evaporated SnO 2 ETL and Cs-containing blended perovskite absorber layer exhibit a power conversion efficiency (PCE) of up to 18.2% without any interface modification. Furthermore, the PSCs demonstrate remarkable long-term stability, which remained at 97% of its initial PCE after being stored for over 34 days. The results demonstrate the great potential of the PSCs based on e-beam evaporated SnO 2 ETLs towards commercialization.
✓ 已验证
产品关联
DOI
FOOD CHEMISTRY
·
2024
·
IF 8.5
Lanqing Yang,Xiaomin Wang,Xu Jing,Baoqing Bai,Tao Bo,Jinhua Zhang,Ligang Yu,Hailong Qian,Ying Gu,Yukun Yang
Escherichia coli O157:H7 ( E. coli O157:H7 ) emerges as a foodborne pathogen, emphasizing the imperative for creating precise detection tools. An ultrasensitive electrochemiluminescence/fluorescence (ECL/FL) dual-signal mode aptasensor was constructed for the detection of E. coli O157:H7 . Ultrafine silver nanoparticles were loaded on the surface of hydrogen-bonded organic skeleton materials by in-situ photoreduction method, and then combined with aptamers that can identify specific targets to prepare HOF-101@AgNPs@Apt, greatly simplifying the ECL/FL dual-signal mode probe fabrication process and improving sensing reliability. HOF-101@AgNPs@Apt had both strong ECL luminescence and fluorescence, resulting in a high detection sensitivity. The low limit of detection (LOD) for ECL and FL were 0.48 CFU mL −1 and 2.39 CFU mL −1 , respectively. Moreover, the proposed dual-signal mode aptasensor has been successfully applied to determine E. coli O157:H7 levels in tap water and milk with superior accuracy and high antiinterference capability, providing a promising method for food safety monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2016
·
IF 3.994
Jinli Li,Xudong Cheng,Yan Chen,Weiming He,Li Ni,Peihua Xiong,Minggang Wei
Luteolin (LUT) is an active agent in cancer prevention and a potential candidate for clinical chemotherapy. However, poor water solubility and extensive excretion have limited the clinical use of luteolin. Herein, we attempted to develop vitamin E d-α-tocopherol acid polyethylene glycol 1000 succinate (TPGS) coated liposomes to improve the accumulation of luteolin in lung cancer. Luteolin or coumarin-6 loaded liposomes were prepared using film-dispersion methods and characterized according to their particle size, polydispersity, zeta potential , and drug encapsulation efficiency. Cellular uptake properties and cytotoxicities of luteolin or coumarin-6 loaded liposomes were determined in A549 cells. Optical in vivo imaging was employed as a method of assessing tumor targeting. The antitumor effect was evaluated in mice xenotransplanted with A549 cancer cells. The results showed that TPGS coated liposomes are spherical, and are ∼176.2 nm in size. TPGS coated liposomes enhanced cellular uptake and apoptosis by upregulating the Bax/Bcl-2 ratio, resulting in improved cytotoxicity in A549 cells. TPGS coated liposomes significantly accumulated in tumor tissue and enhanced tumor inhibition without altering the structures of other organs. Thereby, TPGS coated liposomes provide an effective strategy in cancer chemotherapy for model drugs with poor water solubility such as luteolin.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2015
·
IF 3.313
Huan-Na Lin,Bin-Bin Hu,Ming-Jun Zhu
Clostridium thermocellum can degrade lignocellulosic materials to produce hydrogen , acetate, lactate and ethanol, and surfactants have been proven to have the ability of increasing the enzymatic hydrolysis of cellulosic substrates. Here, the effects of surfactants on the fermentation of spent mushroom compost by C. thermocellum were investigated and their underlying mechanisms were preliminarily studied. PEG8000 and JFC-E had different effects on the fermentation performance of spent mushroom compost. Under optimum conditions, PEG8000 increased the hydrogen production by 59.78%, compared to the control without surfactant, while JFC-E improved the accumulation of glucose and xylose rather than hydrogen yield. PEG8000 also had different impacts on the bioconversion of non-lignin substrates (Avicel, carboxyl methyl cellulose and filter paper). With added PEG8000, the hydrogen production was improved when using Avicel as the substrate, and with PEG8000, the yields of products and sugar accumulation were different from those without PEG8000, indicating that PEG8000 may increase the hydrogen yield by shifting the microbial metabolism.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）