JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2020
·
IF 3.209
Chunhe Yu,Dongmiao Qin,Xiaohua Jiang,Xiangfei Zheng,Biyang Deng
In this work, fluorescent N-doped carbon quantum dots (N-CQDs) have been synthesized by simple hydrothermal heating of natural osmanthus fragrans, without any toxic ingredients or surface chemical modifications. The N-CQDs possess a high quantum yield of 21.9 %, outstanding blue fluorescence, good water dispersity, and excellent optical stability. Because the favorable inner filter effect (IFE) between N-CQDs and quercetin (QT) occurs, the addition of QT to N-CQDs can cause their fluorescence quenching. When Al 3+ was added to the N-CQDs/QT system solution, it was found that the inhibition of IFE leads to the fluorescence intensity of N-CQDs/QT system enhancement by virtue of a specific binding of QT to aluminum ion (Al 3+ ). Therefore, we used the N-CQDs as a novel off-on fluorescent nanosensor to detect QT and Al 3+ . Under optimal conditions, the fluorescent nanosensor can detect QT within the wide linear response in the range of 0.003−80 μmol/L with as low as 1 nmol/L detection limit. For the detection of Al 3+ , the N-CQDs/QT system showed linearity response toward Al 3+ in a range of 0.1∼100 μmol/L and the limit of detection was found at 26 nmol/L. In addition, N-CQDs have been successfully used to efficient quantification QT in human plasma and monitor Al 3+ in serum samples. Noteworthy, the N-CQDs demonstrated low toxicity toward T24 cells, which realized sensing QT and Al 3+ in the living cells.
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FOOD CHEMISTRY
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2020
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IF 6.306
Yanjing Yun,Zhisong Lu,Jing Yang,Taotao Liang,Gang Xiao,Yan Qiao,Yang Liu
Aflatoxin B 1 (AFB 1 ) contamination causes huge economic losses. To explore the correlation between catalase (CAT) and AFB 1 production during fungal development, we fabricated an electrochemical CAT-activity sensor by measuring residual H 2 O 2 after enzymatic degradation. The sensor made by palladium nanoparticles/carbonized bacterial cellulose nanocomposites exhibits a linear range over 0.5–3.5 U/mL and a detection limit of 0.434 U/mL. Both dry weight and CAT activity of mycelia continuously increase. But, the latter shows a greater increase than the former after three days. Specific CAT activity in crude enzyme extract of A. flavus was quantified. It maintains at ~25.00 U/mg for 3 days and enhances to 28.91 and 45.30 U/mg, respectively, on days 4 and 5. AFB 1 production follows the same trend. On days 4 and 5, AFB 1 concentration reaches 201.35 and 767.9 ng/mL, respectively. The positive correlation between specific CAT activity and AFB 1 production suggests that CAT is involved in AFB 1 biosynthesis.
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MATERIALS CHEMISTRY AND PHYSICS
·
2020
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IF 3.408
Tao Guo,Ting Xu,Wentao Xia,Andrew J. Carrier,Li Wang,Xu Zhang
We report herein, double quantum dot composites of graphene oxide and CuO (GOQD-q-CuO), formed by a low-temperature solution process, possess enhanced haloperoxidase (HPO)-like catalytic activity, which catalyze the oxidation of Cl − by H 2 O 2 to generate reactive chlorine species (RCS). A sensitive and selective colorimetric sensor was then developed via the oxidation of 3,3ʹ,5,5ʹ-tetramethylbenzidine (TMB) by the GOQD-q-CuO catalytic system through the generation of RCS for H 2 O 2 and glucose detection, with detection limits of 0.5 and 2.5 μM and linear ranges between 2.5–200 and 5–400 μM, respectively. This sensor was further applied to glucose detection in clinical serum samples showing no significant differences with the hexokinase method. Additionally, we demonstrate that the GOQD modulates the catalytic activity of q-CuO by reversing its zeta potential , producing an overall negatively charged surface, therefore GOQD-q-CuO has much higher affinity than CuO to the positively charged chromogenic substrate TMB, yielding higher HPO-like activity. Moreover, the presence of Cl − accelerates electron transfer to facilitate Cu 2+ reduction to Cu + on the GOQD-q-CuO surface, speeding up the overall catalytic reaction for the RCS generation, therefore, higher detection sensitivity was achieved.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2020
·Vol. 101
·期 7
·2930-2939
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IF 2.614
Xi Yang,Yuan-Yuan Zang,Sha Yang,Zhi-Gang Chen
BACKGROUND Porphyra haitanensis now faces serious heavy metal pollution problems. Natural deep eutectic solvents (NADESs) have been recognized as a novel class of sustainable solvents, which can be used for heavy metal removal. In this study, 28 kinds of NADESs were prepared and investigated as eluent in the removal of lead (Pb), cadmium (Cd), chromium (Cr), arsenic (As), and copper (Cu) from P. haitanensis for the first time, and the adsorption mechanism of NADESs was also studied. RESULTS The removals were greatly improved by NADESs compared with control where the removal rates of Pb, Cd, Cr, As and Cu were 17.4–87.54%, 57.54–100%, 9.8–48.59%, 21.32–78.24% and 11.68–79.73%, respectively. The optimal condition was 10% water content and solid–liquid ratio of 1:20. Moreover, the addition of 20% natural surfactant arabic gum can further increase the heavy metals removal rates of NADESs. The adsorption mechanism experiments showed that the pseudo second-order model and the Freundlich adsorption model can better explain the adsorption mechanism of NADESs on heavy metals removal. CONCLUSION Taken together, a green and efficient method for removing heavy metals from P. haitanensis was established. © 2020 Society of Chemical Industry
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CATALYSIS LETTERS
·
2020
·Vol. 151
·期 7
·1894-1901
·
IF 2.482
Jiang Peng,Fu Haoyue,Ma Hongfang,Qian Weixin,Zhang Haitao,Ying Weiyong
Highly active Ga 2 O 3 nanoparticles catalysts were prepared by one-step oxidative decomposition of gallium nitrate with sucrose-derived carbon foams (SCF) template. The catalysts were investigated by XRD, Ar physisorption, SEM–EDS, HRTEM, TGA, NH 3 -TPD and 71 Ga MAS NMR. The addition of sucrose facilitated the higher surface area of Ga 2 O 3 nanoparticles and morphological transformation from bulk to flake due to the structure-directing role of the sucrose solution. Moreover, the weak-medium surface acid sites increased by adding sucrose properly, which was related to the larger number of tetrahedral Ga 3+ cations. The optimum catalytic activity was achieved over S X CF-Ga 2 O 3 catalysts at the sucrose/Ga 2 O 3 molar ratio of 6 with the propane conversion of 46.63% and propene selectivity of 88.18%. Graphic abstract
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BIOSENSORS & BIOELECTRONICS
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2020
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IF 10.257
Bingfang Wang,Yuanyuan Luo,Lei Gao,Bo Liu,Guotao Duan
Accurate detection of glucose is essential for the diagnosis of diabetes, wherein effective and sensitive biosensors for glucose detection are needed. Here, we fabricated a glucose sensor based on field-effect transistor (FET) with bimetallic nickel-copper metal-organic frameworks (Ni/Cu-MOFs) as its channel layers which were grown in-situ through a simple one-step hydrothermal method and modified with glucose oxidase (GOD) by using glutaraldehyde (GA) as linkers. Due to the synergistic effect of Ni ions and Cu ions in MOFs, the sensor (GOD-GA-Ni/Cu-MOFs-FET) showed good field effect performance and great responses to glucose through enzymatic reactions . It displayed a piecewise linear relationship in the wide range (1 μM–20 mM), and provided high sensitivity (26.05 μAcm −2 mM −1 ) in the low concentration (1–100 μM) and a low detection limit (0.51 μM). The sensor also had these advantages of high specificity, excellent reproducibility, good short-term stability and fast response time. Especially, it is indicated that the Ni/Cu-MOFs-FETs with high performance have the potential to be available sensors, paving the way for the application of bimetallic MOFs in biosensing.
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JOURNAL OF MEMBRANE SCIENCE
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2021
·
IF 8.742
Yu-Jie Shen,Qing-Ran Kong,Li-Feng Fang,Ze-Lin Qiu,Bao-Ku Zhu
Nanoscale permeation channels are a key factor for molecules or/and ions separation in membrane field. In this work, molecular deposition strategy is designed to construct high-performance nanochannel separation layer for selective separation of small molecules. Herein, octaammonium polyhedral oligomeric silsesquioxane (8NH 3 Cl-POSS) and tannic acid (TA), two kinds of multifunctional molecules with a size of about 1 nm, were used to prepare robust and perm-selective composite membrane through covalently-bonded layer-by-layer (LbL) self-assembly method. The optimized TA-POSS composite membrane exhibited a narrowed pore size distribution from 0.3 to 1.6 nm with a high water permeance of 21 L m −2 h −1 bar −1 . The membrane showed desirable rejection of antibiotics (>90%) and enabled to separate them from NaCl solution. In addition, TA-POSS composite membrane showed outstanding stability due to the high degree of crosslinking between TA and 8NH 3 Cl-POSS and their rigid structures. This work gives a novel and green strategy to construct nanochannel composite membranes, which can efficiently separate molecules and salt.
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Drug Design Development and Therapy
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2020
·
IF 3.216
Xuan Zou,Yang Wang,Yuheng Wang,Junting Yang,Huishu Guo,Zhengxu Cai
Introduction Paeoniflorin is a main active component in traditional Chinese medicine. Paeoniae alba radix is widely used as a spasmolytic and pain-relieving agent for abdominal spasmodic pain. Functional dyspepsia (FD) is characterized by pain or burning in the epigastrium, fullness, bloating and nausea. However, limited information is available about the effect of paeoniflorin on FD. Materials and Methods In this study, iodoacetamide or clonidine-induced FD rat models were established to investigate the impacts of paeoniflorin on FD induced by different pathophysiologic disturbances. Results We found the therapeutic effect of paeoniflorin through assessing the gastric emptying, gastric accommodation and visceral hypersensitivity. This function of paeoniflorin was related to the release of acetylcholine (ACh), which was accompanied by reduced acetylcholinesterase (AchE) activity in stomach and hypothalamus. Paeoniflorin administration inhibited the cyclo-oxygenase-2 (COX-2) expression and increased the level of ghrelin in the stomach. Besides, the levels of occludin and ZO-1 were elevated in the duodenum from paeoniflorin-treated rats, suggesting the impaired duodenal barrier was ameliorated. Discussion These results indicate that paeoniflorin possesses the ability to alleviate functional dyspepsia.
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Drug Testing and Analysis
·
2020
·Vol. 13
·期 3
·720-728
·
IF 2.903
Cui-Mei Liu,Hong-Yuan He,Lin Xu,Zhen-Dong Hua
Performing fast qualitative identification of seized illegal drugs by Raman spectroscopy is challenging due to fluorescence interference as well as chemical complexity. Spectrometers with 785-nm excitation, 1,064-nm excitation, and sequentially shifted excitation (SSE) were compared for their effect on fluorescence reduction. The characteristic peaks method, which is independent of cutting agents, was tested as a new strategy to broaden the application of the Raman technique. The suitability of the characteristic peaks method was fully examined by analyzing a large amount of seized illegal drugs, including 72 methamphetamine hydrochloride (concentration range of 13.9%–99.4%), 68 ketamine hydrochloride (17.7%–99.8%), 176 heroin hydrochloride (5.2%–79.5%), 51 cocaine hydrochloride (21.1%–94.5%), and 33 cocaine base (30.9%–92.5%) samples. The results showed that seized methamphetamine, ketamine, and cocaine samples had no or little fluorescence. Hence, in regard to detection of these samples, the advantage of using 1,064-nm excitation and SSE compared with 785-nm excitation was quite limited. Regarding the heroin samples, a significant improvement of the “high” confident positive detected rate was evident for 1,064 nm excitation (60.8%) and SSE (61.4%), compared with 785-nm excitation (13.1%). However, it was also seen that even if 1,064-nm excitation and SSE were applied, the fluorescence of heroin samples was still unable to be fully overcome. By using the characteristic peaks method, low LOD results of 5%–20% were acquired for 40 types of drug mixtures, and lower LODs were obtained for the 60% of the drug mixtures compared with library searching method. Raman spectroscopy in conjunction with the characteristic peaks method was shown to be fast, simple, accurate, and sensitive in the qualitative analysis of seized drug samples.
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MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Na Guo,Yong-Peng Zou,Hong-Kun Li,Ping Kou,Zhi-Ming Liu,Yu-Jie Fu
Conventional approaches for extraction and enrichment of phytochemicals from plant matrix have many drawbacks such as high organic solvent consumption, low extraction yields, time-consuming and large labor for the enrichment process. In the present study, ultrasound-assisted deep eutectic solvents extraction (UAE-DESs) was developed for extraction and determination of the linarin from Chrysanthemum indicum L. flower (CIF). And a feasible strategy was performed to separate and recover the linarin from denatured DESs using AB-8 macroporous resins as adsorbents. Under the optimal extraction conditions, the maximum linarin extraction yield reached 14.23 mg/g with liquid–solid ratio 32 mL/g, extraction power 340 w, extraction time 32 min and deep eutectic solvent of choline chloride-ethylene glycol with the molar ratio 1:2 and water content 30% (v/v) as a extraction system, which was 1.21 times higher than 80% ethanol extraction. After macroporous resins enrichment, the recovery efficiency of linarin reached 81.55%. In addition, the DESs could be regenerated for extraction process, and macroporous resins could be further used for enrichment process with desired performance. This study provided an effective and convenient extraction and recovery method for linarin determination from CIF which was also applicable to other natural plants.
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CHEMICAL ENGINEERING SCIENCE
·
2020
·
IF 3.871
Wei Zhu,Wenquan Huang,Liping Ye,Yuehua Deng,Qiuling Xie,Yanbin Jiang
Zein nanoparticles have good prospects as a pharmaceutical carrier but limited in further application by its poor stability and re-dispersibility. To obtain high stability and pH-responsive drug delivery, succinylated-zein (Suc-zein) was synthesized by nucleophilic substitution reaction, the material obtained was used to prepare Suc-zein nanoparticles (SZs). The SZs prepared not only had good dispersibility but showed better stability than zein particles on pH, storage and salinity. After loading indomethacin (Indo) into zeolitic imidazolate framework-8 (ZIF-8), the core-shelled Indo-Suc-zein-ZIF-8 (Indo-SZZs) were fabricated by coating Suc-zein onto the surface of ZIF-8 with better stability and high drug loading. Indo-SZZs were pH-responsive and sustained a drug release profile in a simulated environment. Also SZZs presented as being non-toxic, having good biocompatibility and good cellular up-take performance, and the cytotoxicity of Indo-SZZs was significantly higher than that of Indo. This provides a new insight into the preparation of a zein-based oral drug delivery system.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
·
IF 3.232
Yi Wang,Chaoqin Han,Lan Yu,Jiangling Wu,Yuanhong Min,Juan Tan,Yannan Zhao,Pu Zhang
Numerous methods have been developed for glucose detection, only few cases can be really applied in clinical diagnosis. Herein, we report a new approach to achieve the detection of glucose in clinical samples and distinguishing the diabetic patients with healthy ones. Specifically, a fluorescence resonance energy transfer (FRET) system is established first, where nitrogen-doped carbon dots (N-CDs) and Ag nanoprisms (AgNPRs) with good spectral overlap act as energy donor and acceptor, respectively. Then, the FRET can be inhibited through oxidative etching of the energy acceptor in the presence of glucose and glucose oxidase , where hydrogen peroxide is generated to transform AgNPRs into Ag + ions. Based on the turn-on fluorescent signal versus glucose concentration, a new method for quantitative detection of glucose is developed. This etching-induced analytical method is simple, reliable, robust and cost-effective, which is promising to assist the doctors to clinically diagnose diabetes and other diseases related to metabolic disorders.
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