EUROPEAN JOURNAL OF PHARMACOLOGY
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2025
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IF 4.7
Xingfang Zhang,Liang Gao,Jianv Wang,Wei Zhang,Dong Xu,Yanhua Wang,Tianlong Liu,Kai Gao,Zhen Ren,Yi Ding
β-Bisabolene, a bioactive sesquiterpene extracted from myrrh—a substance utilized for ischemic stroke treatment—has known therapeutic potential. Nevertheless, the exact mechanisms underlying its beneficial effects on ischemic stroke, along with its potential targets, remain to be fully elucidated. This study aimed to investigate the therapeutic potential of β-Bisabolene in ameliorating ischemic stroke, identify its potential targets and interactions, and explore the underlying pathways involved. Bioinformatics analyses, including network pharmacology and analysis of GEO transcriptomic datasets, were performed to predict the potential targets and mechanisms of β-Bisabolene in ischemic stroke treatment. Preliminary computational validation was achieved through molecular similarity comparisons, molecular docking studies, and molecular dynamics simulations. In vitro experiments were then conducted to confirm the protective effects of β-Bisabolene on microglia injured by oxygen‒glucose deprivation and to validate the findings from the bioinformatics analyses. The results revealed that β-Bisabolene reduces the levels of the proinflammatory cytokines TNF-α, IL-1β and NO; decreases reactive oxygen species levels; and decreases the expression of COX-2, P38, ERK and Keap1. Moreover, it increases the expression of Arg-1, Nrf2, NQO1 and HO-1. These effects are associated with improved survival rates of oxygen–glucose deprivation–damaged microglia. In conclusion, β-Bisabolene may exert anti-inflammatory and antioxidant effects to ameliorate microglial injury induced by ischemic stroke by antagonizing COX-2 and mediating the MAPK signaling pathway and Keap1/Nrf2 pathway. This study provides valuable insights into the therapeutic potential of β-Bisabolene for the treatment of ischemic stroke.
研究领域:
药理学
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神经科学
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生物信息学
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产品关联
DOI
PubMed
高影响因子
Environmental Science-Processes & Impacts
·
2025
·
IF 3.9
Xiaomin Ren,Jianying Wang,Fenqing Zhao,Pingping Zhang,Huan He,Zhixiang Xu,Bin Huang,Xuejun Pan
Previous studies have indicated that 6:2 chlorinated polyfluoroalkyl ether sulfonate (6:2 Cl-PFAES), a substitute for perfluorooctane sulfonate (PFOS), caused disruptions in glucose metabolism, but its toxicological mechanisms remain unclear. Pancreatic β cells are responsible for regulating glucose metabolism. This study used mouse insulinoma β cells (β-TC-6) to study the toxic effects of 6:2 Cl-PFAES and elucidate its potential mechanisms. Through cell counting kit-8, trypan blue staining, and apoptosis assays, we found that 6:2 Cl-PFAES showed toxicity to β-TC-6 cells, even surpassing that of PFOS. Mechanistic studies demonstrated that 6:2 Cl-PFAES induced oxidative stress by ascendant levels of reactive oxygen species (ROS) and malondialdehyde (MDA), alongside increased superoxide dismutase (SOD) activity. The oxidative stress-inducing capability of 6:2 Cl-PFAES was stronger than PFOS. Co-exposure with N-acetyl-L-cysteine (NAC), as the ROS scavenger, which obviously reduced the degree of cell apoptosis and the production of ROS, highlighting oxidative stress as a crucial mechanism of 6:2 Cl-PFAES-induced cell death. Transcriptomic analysis further revealed that 6:2 Cl-PFAES affected genes association with the oxidative phosphorylation pathway, confirming the oxidative stress effect, and altered diabetes-related genes expression, suggesting a heightened risk of diabetes. This study provides insights into the toxicity of 6:2 Cl-PFAES on β cells and its potential risks on glucose metabolism disruption.
研究领域:
毒理学
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分子生物学
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环境健康科学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2018
·Vol. 6
·期 32
·15789-15796
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IF 9.931
Jiali Sheng,Jiahui Kang,Zhixiong Hu,Yan Yu,Xian-Zhu Fu,Rong Sun,Ching-Ping Wong
Exploring effective electrocatalysts is in urgent demand to boost their large-scale applications for direct ethanol fuel cells. Herein, for the first time, we report nanoparticle-assembled Pd nanocages with efficient catalytic activity towards ethanol electrochemical oxidation. The catalysts are prepared from the second template of a nanosheet surface of amorphous Co(OH)2 octahedral nanocages, which are prepared from the original template of octahedral Cu2O. These Pd octahedral nanocages exhibit a porous rough surface, which consists of smaller Pd nanoparticles, endowing them with highly active surface area and many active sites. For the electrochemical oxidation of ethanol, the Pd nanocages demonstrate superior activity (3765 mA mgPd−1), 4.8 times and 5.9 times those of commercial Pd/C and Pd nanocatalysts, respectively, prepared directly from an octahedral Cu2O original template. Furthermore, the Pd nanocages demonstrate excellent long-term durability.
研究领域:
材料科学
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电化学
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能源科学
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DOI
高影响因子
ACS Pharmacology & Translational Science
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2025
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IF 3.7
Yizhen Guo,Ding Huang,Yan Zhou,Maoxin Ran,Shaolin Zhang,Kin Yip Tam
Acquired resistance to osimertinib (OSI) poses a significant challenge in the treatment of epidermal growth factor receptor mutant non-small cell lung cancer (NSCLC). Although OSI is effective as a first-line salvage therapy in T790M-positive patients following progression on first- or second-generation EGFR-TKIs (erlotinib, gefitinib, and afatinib), the inevitable development of acquired resistance limits its therapeutic efficacy. This study reveals that OSI-resistant (OSIR) NSCLC cells underwent metabolic reprogramming characterized by enhanced glycolysis and upregulation of hexokinase 2 (HK2). We demonstrated that HK2 inhibitor, benserazide, exhibited significant anticancer effects in OSIR cell models and mediated by reactive oxygen species. Our results suggested that HK2 inhibition effectively modulated the enhanced glycolysis, activated the AMPK-mTOR-autophagy axis, and unexpectedly interfered with NF-κB signaling through direct HK2-IKKβ interaction. Excitingly, the protein expression level and activity of pyruvate dehydrogenase kinase 1 (PDK1) in OSIR cells were upregulated upon HK2 inhibition, indicating a pro-survival role. Combined inhibition of HK2 and PDK1 synergistically inhibited the proliferation of OSIR cells and significantly suppressed tumor growth in an OSIR cell xenograft model, outperforming the single use of HK2 inhibitor. This combination successfully rectified aberrant glucose metabolism and enhanced oxidative phosphorylation. Our findings identified HK2 as a crucial mediator in overcoming OSI resistance and suggested that combined inhibition of HK2 and PDK1 could be a promising approach in OSIR NSCLC.
研究领域:
肿瘤学
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分子生物学
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药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
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2022
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IF 4.331
Yafeng Wang,Xian Luo,Wenjie Lu,Bin Huang,Yanqing Yang
In this study, β-Ni(OH)2 with a unique flower-like morphology was synthesized through a hydrothermal method. The doping of Rh in β-Ni(OH)2 was achieved by a reduction method. The as-synthesized catalysts were characterized by X-ray diffraction, transmission electron microscopy, and X-ray photoelectron spectroscopy for the crystal structure, morphology, composition, and chemical state analysis. The electrochemical tests revealed that the doping of Rh can significantly increase the electrocatalytic performance of β-Ni(OH)2 in 1.0 M KOH solution. The methanol oxidation peak current density of Rh-doped β-Ni(OH)2 reached 95 mA cm–2 with a Tafel slope of 40 mV dec–1. The reason for these improvements is that Rh can suppress the phase transformation of NiOOH from β to α. Meanwhile, the electronic structure change of nickel in β-Ni(OH)2 and the defect ratio increase caused by Rh doping are beneficial to methanol oxidation reaction (MOR) catalytic activity. Furthermore, the synergistic effect between Rh and Ni(OH)2 improved the surface activity of β-NiOOH. The doping of Rh in β-Ni(OH)2 is initiative in this work, which provides a new strategy to design highly efficient and cost-effective MOR electrocatalysts for alkaline DMFCs.
研究领域:
材料科学
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电化学
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DOI
高影响因子
JOURNAL OF CHEMICAL RESEARCH
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2018
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IF 0.668
Xiaoyun Ma,Daqin He,Zhengjian Chen
A simple method for the preparation of nitriles in moderate to good yield has been achieved by treatment of aromatic and heterocyclic aldoximes with malononitrile in water at reflux in the presence of copper acetate as catalyst. Arylaldoximes with an electron-donating group showed the highest reactivity, their conversion being achievable at room temperature.
研究领域:
有机化学
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合成化学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2025
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IF 10.5
Xue Fan,Lujie Wang,Hongxin Wang,Liuyi Huang,Jiahui Lin,Xia Gao,Fengna Xi
An immunosensing platform was developed based on the enhanced electrochemiluminescence (ECL) of luminol-dissolved oxygen (O 2 ) by nanochannel-confined Ni(OH) 2 -CeO 2 composite nanozyme, which is able to sensitively detect cytokine. The mesoporous silica nanochannel array film (SNF), decorated on a cost-effective ITO electrode, features ultrasmall (2∼3 nm) nanochannels, enabling the confinement of in situ synthesized Ni(OH) 2 -CeO 2 composite nanozyme via a continuous electrodeposition process. Ni(OH) 2 -CeO 2 exhibits dual peroxidase (POD) and oxidase (OXD) enzyme-like activities, serving as an efficient oxygen reduction reaction (ORR) catalyst to produce reactive oxygen species (ROS) and catalyze luminol oxidation. Compared to nanozymes synthesized on flat electrodes, nanochannel-confined nanozyme demonstrates superior ECL enhancement. The combination of Ni(OH) 2 and CeO 2 exhibits strong synergistic catalytic performance, boosting ECL of luminol-O 2 under neutral conditions by 33.7 orders compared to electrode without confined nanozyme. Using tumor necrosis factor-alpha (TNF-α) as a proof-of-concept demonstration, immunosensor is fabricated by immobilization recognition antibodies on the SNF outer surface. TNF-α binding induces immunocomplex formation, reducing ECL signal by increasing interfacial resistance and hindering luminol diffuse. This enables sensitive TNF-α detection over a wide linear range (10 fg/mL to 10 ng/mL) with an ultralow detection limit of 8 fg/mL. The immunosensor demonstrates good selectivity, stability, and reproducibility.
研究领域:
生物医学工程
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Linjing Su,Jingyan Xu,Bingfeng Yu,Xionghui Ma,Zhi Zhang,Yuhao Xiong
The detection of 2-mercaptobenzothiazole (MBT), a widely used industrial compound posing significant environmental and health risks, demands efficient and practical solutions. Here, we present a novel colorimetric sensor integrating photoinduced gold nanoparticles (AuNPs) formation inhibition with salt-assisted liquid–liquid microextraction (SALLME) for rapid and sensitive MBT detection. This approach eliminates the need for AuNP seeds, enabling simple and cost-effective analysis at room temperature. The sensor demonstrated a wide linear range (5–640 μM, R 2 = 0.990) and an impressive limit of detection (LOD) of 0.024 μM, outperforming several conventional methods. Spiked recovery tests using river water and soil samples yielded recovery rates of 94.0–107.5 % with relative standard deviations below 7.1 %, confirming its analytical accuracy and robustness in complex matrices. This study offers a promising pathway for developing practical MBT detection techniques with broad environmental applicability.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
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IF 5.4
Xiaoyu Qiu,Linting Zou,Jianmei Cen,Xingnian Duan
In this research, Mo-oxides complexes combined with Co(OH) 2 grown on three-dimensional (3D) nickel foam (Mo-Co(OH) 2 /NF) were developed as a peroxymonosulfate (PMS) activator enabling a pathway transition from radical to non-radical for efficient degradation of organic pollutants. The incorporation of high-valence Mo species promoted the formation of sheet structure and facilitated the Co(II)/Co(III) ratio, while Co served as the primary active site for PMS activation. Consequently, complete removal of 40 mg/L Rhodamine B (RhB) in optimized conditions (catalyst = 1.5 cm 2 , 0.4 g/L PMS dosage, reaction temperature = 25 °C) was achieved within 2 min. In addition, excellent adaptability was exhibited over a wide pH (3.0–9.0) and versatility (99 %, 80.94 % and 91.54 % for MB, LFX, and TC, respectively) in Mo-Co(OH) 2 /NF + PMS system. Impressively, radical scavenging experiments and electron spin resonance (ESR) analyses revealed that the degradation process was mainly dominated by the conversion of O 2 •– to non-radical 1 O 2 . Hence, this work demonstrates the potential of high-valent metal-oxides complexes integrated with Co(OH) 2 as effective PMS activators for non-radical degradation of dye and other refractory antibiotics, significantly broadening their applications in environmental remediation.
研究领域:
环境科学
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材料科学
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化学工程
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2023
·
IF 2.6
Lingli Zhang,Chunjuan Huang
The equilibrium solubility of 6-bromo-2-methylquinoline in various binary blends [ethanol/isopropanol/N,N-dimethylformamide (DMF)/methanol + water] and ten monosolvents (methanol, ethanol, n-propanol, isopropanol, ethyl acetate, toluene, acetone, acetonitrile, DMF, and water) was studied by the isothermal method at 278.15–323.15 K under 101.2 kPa. Among the ten pure solvents, the maximum solubility in mole fraction was obtained in toluene (3.318 × 10–2), followed by ethyl acetate (2.991 × 10–2), acetone (2.764 × 10–2), acetonitrile (2.528 × 10–2), DMF (2.220 × 10–2), n-propanol (9.141 × 10–3), isopropanol (8.123 × 10–3), ethanol (3.896 × 10–3), methanol (9.981 × 10–4), and water (2.888 × 10–5) at 323.15 K. In four solvent mixtures, the maximum and minimum solubilities were obtained in the (DMF + water) and (methanol + water) blends. The solubility of 6-bromo-2-methylquinoline in pure solvents was only affected by temperature and calculated by the Apelblat, van’t Hoff, and NRTL models. The Jouyban–Acree (J–A), van’t Hoff–Jouyban–Acree (V–J–A), and modified Apelblat–Jouyban–Acree (A–J–A) models were used to evaluate the solubility data in mixed systems. The calculation of solvent effect deeply explored the dissolution behavior of 6-bromo-2-methylquinoline from the molecular point of view, and the results showed that the interaction between the solute and solvent was dominant. The apparent thermodynamic properties of 6-bromo-2-methylquinoline in pure solvents were obtained by van’t Hoff plots.
研究领域:
化学
·
物理化学
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DOI
高影响因子
JOURNAL OF SOLUTION CHEMISTRY
·
2022
·Vol. 52
·期 1
·91-104
·
IF 2
Yu Yingmin,Yi Lingxiao,Wei Jiajun,Guo Hongyue,Li Min
The liquid–liquid equilibrium (LLE) data for ethylene glycol + 2-ethyl-1-hexanol + { n -hexane, 1-hexene, 2-propyl-1-heptanol, and isopropyl ether} were measured at 298.2 K under 101.3 kPa. The distribution constant ( D ) and selectivity ( S ) of 2-ethyl-1-hexanol in the systems were calculated to analyze the extraction ability of the four extractants. The Bachman and Othmer–Tobias equations were chosen to examine the experimental LLE data. The linear correlation coefficient R 2 is greater than 0.98, indicating that the linear fitting of the experimental data is good. The data were regressed by thermodynamic models NRTL and UNIQUAC. The deviation between experimental data and simulated data was calculated by the root mean square deviation (RMSD) and the absolute average deviation (AAD). The RMSD is less than 0.79% and the AAD is less than 0.46%. It indicates that the two models are suitable for the regression of the system.
研究领域:
化学工程
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热力学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
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IF 4.784
Qiang Chu,Ruoyi Jia,Wen Chen,Yangyang Liu,Yonglu Li,Xiang Ye,Yong Jiang,Xiaodong Zheng
Ethyl Carbamate (EC), as a carcinogen widely found in fermented foods, was verified that its cytotoxicity was associated with oxidative stress. Polysaccharides from natural sources due to their antioxidative capacity have attracted great attention in the past time. In this study, purified polysaccharide from Tetrastigma hemsleyanum vines (TVP) with 64.89 kDA was extracted and conducted multiple analysis to identify its structural information. It could be discovered that TVP was composed of mannose, rhamnose, glucuronic acid, glucose, galactose, and arabinose. In vitro , TVP could inhibit cytotoxicity and genotoxicity, attenuate oxidative damage and mitochondrial dysfunction induced by EC in Caco-2 cells. Meanwhile, TVP could suppress apoptosis by mTOR and Bcl-2 signaling pathways, ameliorate oxidative via Sirt1-FoxO1 and Nrf2-Keap1 signaling pathways. In vivo , EC as well triggered the decline of survival and athletic ability in Caenorhabditis elegans ( C. elegans ) and TVP could reverse the decline. In the meantime, TVP could ameliorate oxidative damage in N2 and daf-2 (-) mutant but fail in daf-16 (-) mutant, which suggested that DAF-16 (FOXO) might affect the antioxidative protection of TVP in C. elegans . In brief, our results manifested that TVP could attenuate EC-induced cytotoxicity both in vitro and in vivo .
研究领域:
药理学
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毒理学
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分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）