Food Analytical Methods
·
2019
·Vol. 12
·期 6
·1429-1438
·
IF 2.413
Duan Hui-Ling,Mou Zhao-Li,Wang Jun,Ma Shi-Yao,Zhan Han-Ying,Zhang Zhi-Qi
Two types of magnetically modified porous β-cyclodextrin polymers (PCDPs) were successfully synthesized. The first type, named MPCDPs(C), was fabricated by co-precipitating PCDPs with Fe 3 O 4 , resulting in a Brunauer–Emmett–Teller (BET) surface area of 106.68 m 2 g −1 . The second type, named MPCDPs(M), was fabricated by grafting PCDPs onto the surface of magnetic microspheres, resulting in a BET surface area of 34.63 m 2 g −1 . Both MPCDPs were found to enable effective extraction and separation of Sudan dyes from foodstuffs and environmental water. Coupled with high-performance liquid chromatography, the limits of detection for Sudan dyes were in the range of 0.013–0.054 ng mL −1 with MPCDPs(C), and 0.028–0.039 ng mL −1 with MPCDPs(M). Recoveries were between 85.8 and 102.8% for spiked food samples and 88.3 and 103.2% for spiked water samples, suggesting that the developed methods are promising for accurate quantification of Sudan dyes at trace levels in foodstuffs and environmental water.
研究领域:
分析化学
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食品科学
·
环境科学
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2016
·
IF 3.858
Fu Liu,Chuan-He Tang
Formulation of food-grade Pickering emulsions has attracted growing attention in the food and drug fields, due to their promising potential as novel delivery systems for lipophilic bioactive ingredients. The present work reported that a kind of gel-like Pickering emulsions stabilized by heat (at 100 °C)-treated soy glycinin (SG; a major globulin in soy proteins), fabricated by modulating protein concentration ( c ) and/or oil fraction ( ϕ ), can perform as sustained release delivery systems for β -carotene. Rheological measurements and microstructural observations indicated that increasing c and/or ϕ resulted in a progressive strengthening of gel-like network in the emulsions stabilized by heated SG, which was closely related to a high percentage of proteins adsorbed at the interface, or entrapped within the network. In contrast, no distinct gel formation was observed for the emulsions stabilized by unheated SG. In vitro intestinal digestion experiments showed that for the emulsions (stabilized by unheated or heated SG) without distinct gel formation, the release behavior of β -carotene was mainly determined by droplet size , while in the case of gel-like emulsions by heated SG, the gel-like network formation greatly slowed down the β -carotene release (irrespective of the applied c and/or ϕ ). All the test emulsions did not suffer a distinct degradation of β -carotene over the whole digestion process. The findings are of great interest for the development of novel sustained delivery systems for lipophilic components, based on Pickering emulsions stabilized by a kind of protein-based particle stabilizers.
研究领域:
食品科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2025
·
IF 14.3
Kang Wang,Zexin Liu,Lin Liu,Tiantian Wang,Kun Chen,Runhua Yao,Ruiqiang Hang,Xiaohong Yao
Zinc-based materials have been extensively researched as biodegradable bone repair materials in recent years, but their low degradation rate and limited bioactivity need to be further improved. In this study, a new-type dual-sized macro-micro porous Zinc-Magnesium-β-tricalcium phosphate (DMMP Zn-Mg-β-TCP) scaffolds were designed and fabricated by vacuum heating-press sintering. The mechanical properties, degradation behaviors, in vitro biocompatibility, in vivo osteogenesis, and biological safety of the scaffolds were systematically evaluated. The results show that DMMP Zn-Mg-β-TCP has suitable mechanical properties, degradation properties, and excellent osteogenic properties. The compressive yield strength range of the scaffolds is 12.90–25.46 MPa, and the elastic modulus range is 0.31–0.44 GPa, meeting the mechanical properties required for implant materials in cancellous bone. In vitro degradation tests showed that the corrosion rate of the scaffolds ranges from 2.25 to 2.61 mm/y. Furthermore, the scaffolds exhibited excellent in vitro cell compatibility. After a 24-week animal experiment, the scaffold demonstrated excellent osteogenic capacity during bone healing, while maintaining biomechanical stability throughout its degradation process. In summary, the DMMP Zn-Mg-β-TCP has the potential to become a novel biodegradable bone implant material.
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产品关联
DOI
高影响因子
Cancer Management and Research
·
2025
·
IF 2.6
Bo Gao,Baokun Li,Jitao Hu,Xuhua Hu,Mingming Su
Purpose Colorectal cancer (CRC) is a prevalent cancer worldwide, with metastasis significantly contributing to its high mortality and poor prognosis. This study focuses on the impact of Alkaline Phosphatase, Placental (ALPP) on epithelial-mesenchymal transition (EMT) in colorectal cancer cells and its role in the Wnt/β-catenin signaling pathway.Patients and Methods Differential ALPP expression was first interrogated in metastatic versus non-metastatic CRC samples from The Cancer Genome Atlas (TCGA-CRC) cohort. Functional validation was subsequently performed in vitro with HT29 and HCT116 cell lines engineered for ALPP overexpression or CRISPR/Cas9-mediated knockdown. Proliferation, migration and invasion were quantified by CCK-8, wound-healing and Transwell assays; EMT and Wnt/β-catenin signaling were assessed by Western blot.Results Bioinformatics analysis revealed significantly different ALPP expression between metastatic and non-metastatic patients. In vitro experiments further revealed that ALPP overexpression drives proliferation, invasion, migration and, consequently, metastasis of HT29 and HCT116 colorectal cancer cells, whereas ALPP knockdown abolishes these EMT-dependent effects. Increased ALPP expression resulted in increased levels of N-Cadherin, Vimentin, and Snail proteins, along with a decrease in E-cadherin protein expression, in contrast to findings following ALPP knockdown. Furthermore, ALPP overexpression was also associated with Wnt/β-catenin signaling pathway activation.Conclusion ALPP was found to act as an oncogenic factor in colorectal cancer cell lines HT29 and HCT116, stimulating cell proliferation and facilitating EMT. Abnormal activation of the Wnt/β-catenin signaling pathway was also found to be linked to increased ALPP expression.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER SCIENCE AND TECHNOLOGY
·
2018
·Vol. 78
·期 5
·1001-1009
·
IF 1.247
Ding Chuyuan,He Jun,Xu Mengxia,Wang Chengjun
In this work, the novel β -cyclodextrin modified mesostructured silica coated multi-walled carbon nanotubes (MWCNTs) composites were synthesized and applied for the removal of parabens in aqueous solution. The prepared MWCNTs/SiO 2 / β -CD composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy and thermogravimetric analysis. The effects of the amount of adsorbent, pH and elution solvents on the removal efficiency of parabens from water solutions were investigated. Under the optimized conditions, over 95% removal efficiency was achieved by using 40 mg of MWCNTs/SiO 2 / β -CD adsorbents to absorb the parabens from 60 mL of 0.5 μg/mL parabens solutions. The solution pH in the range from 5 to 9 has no influence on the removal efficiency and the parabens sorption capacity of the prepared adsorbents were around 0.75 μg/mg. Furthermore, the stability and reusability studies demonstrated that the prepared MWCNTs/SiO 2 / β -CD composites are cost-effective adsorbents for the removal of parabens from water with high regeneration efficiency. The composites fabricated in this study could become an attractive candidate for water purification.
研究领域:
环境科学
·
材料科学
·
纳米技术
·
分析化学
·
水处理工程
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DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Cheng Zeng,Xiangqing Ji,Yu Shi,Siyu Mu,Yicheng Huang,Meiqiao Zhong,Yan Han,Cuicui Duan,Xiaolei Li,Dan Li
To develop a new method for enzymatic preparation of minor ginsenosides , T. stercorarium β-glucosidase (Tsbgl) was characterized and its activities of deglycosylation towards natural ginsenosides were examined. The substrates of 1 mmol l −1 were incubated with the enzyme of 38.3 U ml −1 at 65 ℃ and pH 5.0. The K m values of Tsbgl for ginsenosides Rb 1 , Rg 1 and p NPG were 0.37 ± 0.03, 3.26 ± 0.19, and 1.24 ± 0.03 mmol l −1 , and the V max values were 183.63 ± 7.15, 85.03 ± 4.90, and 117.66 ± 1.96 μmol mg −1 min −1 , respectively. The molar conversion of ginsenosides Rb 1 , Rb 2 , Rb 3 , Rc, Re, Rg 1 , and Rf by Tsbgl within 6 h was 100%, 50.1%, 42.7%, 92.0%, 57.3%, 67.9%, and 76.8%, respectively. The yield of aglycone protopanaxadiol was 35.5 μmol l −1 h −1 for Rb 1 , while the yields of aglycone protopanaxatriol were 64.2 and 70.4 μmol l −1 h −1 for Rg 1 and Rf. Tsbgl with good organic solvent tolerance, mild reaction conditions and broad substrate specificity , could completely remove all outer glucosyls at the C-3 and C-20 hydroxyls of protopanaxadiol-type ginsenosides, and the C-6 and C-20 hydroxyls of protopanaxatriol-type ginsenosides through various pathways, providing a specific and efficient way to produce minor ginsenosides.
研究领域:
生物化学
·
药理学
·
酶学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Ying Zhang,Wang Ren,Yu Zhu Fan,Hong Qun Luo,Nian Bing Li
Monitoring the concentration of norepinephrine (NE), epinephrine (Ep) and dopamine-β-hydroxylase (DβH) is of great importance in studying and diagnosing related diseases. In this study, a simple chemically-modulated turn-on fluorescence strategy is proposed for rapid, selective and visual discrimination of trace NE and Ep. Herein, Ep is differentiated from other catecholamines via its fast fluorescence response in 1.0 M NaOH medium. Furthermore, NE is alkalized with 0.01 M NaOH and is then immediately triggered by polyethyleneimine to emit bright cyan fluorescence, whereas Ep and DA show no any fluorescence response. The fluorescence intensity of resultant solution is linearly proportional to the concentration of Ep and NE in the range of 50.0–10000.0 nM and 10.0–10000.0 nM, respectively. Moreover, NE and Ep as low as 500.0 nM can be fluorescently identified with naked-eye, which is convenient for rapidly preliminary diagnosis and self-test of hypercatecholaminism. Inspired by the catalytic property of DβH transforming DA into NE, the proposed strategy enables a simple and high-efficient fluorescence method to monitor DβH by using trace DA as substrate and directly quantifying NE. The method is simple, smart, highly efficient and eco-friendly, and has been applied to the rapid assay of NE, Ep and DβH in real samples.
研究领域:
生物化学
·
医学诊断
·
分析化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Jinshui Liu,Guoning Liu,Wenxiu Liu
Novel nanocomposites of β-cyclodextrin/poly(acrylic acid) grafted onto graphene oxide (β-CD/PAA/GO) were successfully synthesized for the first time using an esterification reaction, and they were used to remove the cationic dyes methylene blue (MB) and safranine T (ST) from aqueous solutions. The as-prepared β-CD/PAA/GO nanocomposites were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The β-CD/PAA/GO nanocomposites showed excellent water dispersibility because of the hydrophilicity of the polymer. The adsorption isotherms fitted the Langmuir isotherm model. The maximum adsorption capacities were 247.99 mg g −1 for MB and 175.49 mg g −1 for ST, and these are much higher than those of other carbon-based adsorbents that have been described in the literature. This was attributed to the high concentration of functional groups in the nanocomposites. Repeated MB and ST adsorption and desorption cycles showed that the β-CD/PAA/GO nanocomposites could be used repeatedly, and their adsorption capacities did not change significantly after five cycles. The fast and efficient adsorption of MB and ST by the β-CD/PAA/GO nanocomposites from aqueous solutions suggested that these novel nanocomposites may be ideal candidates for removing dyes during water treatment.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yuan-Yuan Cui,Yan-Ping Bi,Cheng-Xiong Yang
Layer-stacking structures via the template-free synthesis are rare in two-dimensional microporous organic networks (2D MONs). Herein, we report a facile solvent regulation and template-free strategy by tuning the aggregation degree of 2D β-cyclodextrin (CD)-based MONs nanosheets. The 2D MON nanosheets provided larger uptake capacity and faster kinetics than the aggregated CD-MONs, revealing the significance of morphology control for the removal of pollutants. The prepared 2D CD-MON-DMF gave ultrafast adsorption kinetics and exhibited much larger saturation sorption capacity (327.9 mg g −1 ) than previous adsorbents for bisphenol AF. The developed strategy not only provides a novel way to construct 2D MON nanosheets for highly efficient removal of organic pollutants from water but also promotes the utilization of MONs in material and environmental sciences.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2024
·
IF 8.1
Yixuan Zhang,Guo-Qiang Liu,Danlei Qiao,Jiaguan Chen,Qiang Sun,Lei Wen
This work focuses on the effect of the of Mn 3+ /Mn 4+ ratio in the reactants on the structure and electrochemical properties of NaMn 0.9 Cu 0.1 O 2 cathode materials for sodium-ion batteries. By systematically varying the manganese valence states in the reactants, we observed a phase transition from the β-phase to the P2-phase with increasing Mn 3+ concentrations in the reactants, contrary to the expected favorability of the β-phase at higher Mn 3+ levels. This finding suggests a complex interplay between the valence states of the reactants and phase formation mechanisms, which needs further investigation. Additionally, electrochemical testing indicated that the phase composition significantly impacts the electrochemical performance of the material. Specifically, the M0 sample, possessing the optimal P2/β ratio, exhibited excellent electrochemical performances, achieving a high initial discharge capacity of 155.1 mA h g −1 and remains 88.1 % after 40 cycles. These results emphasize the importance of precursor selection and valence management in the development of high-performance cathode materials for sodium-ion batteries.
研究领域:
材料科学
·
电化学
·
能源存储
·
无机化学
·
固态化学
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2018
·
IF 2.082
Jingjing Du,Shuchun Gan,Qihao Bian,Duhan Fu,Yan Wei,Kaiqun Wang,Qiaoxia Lin,Weiyi Chen,Di Huang
In this study, novel porous scaffolds containing hydroxyapatite and β-cyclodextrin-based polyurethane were first successfully fabricated by polymerizing β-cyclodextrin with hexamethylene diisocyanate and hydroxyapatite in situ for bone tissue engineering. The physicochemical and mechanical properties as well as cytocompatibility of porous scaffolds were investigated. The results showed that polyurethane reinforced with hydroxyapatite composites had cancellous bone-like porous structure. The mechanical strength of the scaffolds increased with increasing the hydroxyapatite content in scaffolds. Synthesized scaffolds (PU1, PUHA1, PU2, and PUHA2) presented compressive strength values of 0.87 ± 0.24 MPa, 1.81 ± 0.10 MPa, 6.16 ± 0.89 MPa, and 12.95 ± 2.05 MPa, respectively. The pore size and porosity of these scaffolds were suitable for bone regeneration. Cytocompatibility of composite scaffolds was proven via favorable interactions with MC3T3-E1 cells. The addition of hydroxyapatite into CD-based polyurethane scaffolds improved cell attachment, well-spread morphology, and higher proliferation. The hydroxyapatite-polyurethane scaffolds have the potential to be applied in bone repair and regeneration.
研究领域:
材料科学
·
生物医学工程
·
组织工程
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DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Liang Dong,Hui Chen,Ting Liu,Junming Zhu,Min Yu,Qingmei Yuan
Three disulfide bond-containing peptide amphiphiles (PA1–3) with different lengths of alkyl tails (PA1 for C6, PA2 for C12, and PA3 for C18) were synthesized by ring-opening polymerization of α-amino acid N-carboxyanhydride followed by post-polymerization modification. The peptide segments were composed of 3-mercaptopropionic acid-modified poly(l-cysteine) [P(Cys-SS-CH2CH2COOH)]. We characterized the chemical structure and molecular parameters by 1H NMR, 13C NMR, gel permeation chromatography, Fourier transform infrared spectroscopy, and circular dichroism spectroscopy. It is found that alkyl-P(Cys-SS-CH2CH2COOH) mainly presents a β-sheet conformation at the solid state. However, the PAs present predominant random coils at pH 7.4 in aqueous solutions. The β-sheet conformation increased dramatically when the concentration of the PA exceeded its critical micelle concentration (ca. 0.3 mg/mL for PA3), indicating the formation of self-assembly-induced β-sheet nanostructures. Elongation of the alkyl chain length or a decrease of the pH of the PA solution can promote the formation of the β-sheet conformation. The three PAs can self-assemble into spherical micelles or nanofibrous hydrogels, which can be utilized as nanocarriers for systemic drug delivery or implants for localized drug delivery, respectively. Cisplatin (CDDP) was loaded as a model medicine to examine the drug delivery potential of PA3. We found that the CDDP-loaded PA3 micelles are stable at pH 7.4, have a spherical morphology with a hydrodynamic diameter of ca. 52 nm, and accomplish pH/reduction dual-responsive release of CDDP. In addition, alkyl-P(Cys-SS-CH2CH2COOH) can self-assemble into nanofibrous hydrogels at pH 5.0–6.0 or upon the addition of certain metal ions and show excellent dynamic reversibility. Moreover, the CDDP-loaded PA3 hydrogel exhibits a sustained release profile and a nearly linear release over 48 h. In vitro cytotoxicity of PA3 also indicates its nontoxicity. Thus, our findings suggest that alkyl-P(Cys-SS-CH2CH2COOH) has great potential for both systemic and localized delivery of therapeutics.
研究领域:
生物材料学
·
药剂学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）