INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Na Gan,Qinhong Li,Yuanqiao Li,Mohan Li,Yilin Li,Lijuan Chen,Tingting Zeng,Yali Song,Fang Geng,Di Wu
The volatile characteristics of lemongrass essential oil (LO) have seriously hindered its further application, and encapsulation it with multilayer modified liposomes may be an effective strategy to improve this dilemma. This study selected chitosan (CH) and three anionic polymers, pectin (P) / gum arabic (GA) / carrageenan (C), as the first and second coating polymers to modify nano liposomes (NL) by layer-by-layer electrostatic deposition, obtaining three bilayer liposomes, P-CH-NL, GA-CH-NL, and C-CH-NL as high-quality stabilized carriers of LO. The bilayer liposomes showed a dense membrane structure ranging from 110 to 150 nm uniformly, with good antioxidant properties. All bilayer liposomes had good stability during 28-day storage at 4 °C, while C-CH-NL performed relatively better inferred by smaller changes of size, PDI and Zeta potential. The total volatile base nitrogen (TVB-N) values of fresh chicken meat and a total number of bacterial colonies (TBC) experiments showed that GA-CH-NL and C-CH-NL could better retard the increase of volatile salt base nitrogen. All bilayer liposomes could delay the time for the total bacterial count to exceed 6 log CFU/g (from 7 days to 10 / 12 days). Therefore, the bilayer liposomes P-CH-NL, GA-CH-NL, and C-CH-NL may be promising natural preservatives for food products.
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高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Wen Tao,Diguo Liu,Ziqi Guo,Pengfei Han,Yan Ma,Muci Wu,Rui Zhang,Jingren He
A novel selenylated low molecular weight apple pectin (Se-LMWAP) was prepared through enzymatic modification combined with selenylation. The physicochemical properties, the structural characterization of Se-LMWAP were evaluated by FT-IR, NMR and SEM. Moreover, the antidiabetic activity and potential mechanism of Se-LMWAP were investigated using high-fat diet (HFD)/streptozotocin (STZ)-induced type 2 diabetic mice. The results revealed that the physicochemical properties of Se-LMWAP were distinctly improved after modifications, and the primary structure was not altered significantly compared to apple pectin and low molecular weight apple pectin. Se-LMWAP had a relative molecular weight of 8.91 × 10 3 Da (accounted for 61.3 %) and total selenium content of 148.3 ± 2.0 μg selenium /g sample . It consisted of Rha, Ara, Gal, Glc, Xyl and GalA at a molar ratio of 0.093:0.014:0.132:0.020:0.118:0.622, with the selenium substitution occurred at the C-6 position. Se-LMWAP was able to significantly reduce weight loss, hyperglycemia, oxidative stress and liver, kidney and pancreas damage. Additionally, improved glucose tolerance, relieved lipid metabolism disorders, elevated hepatic glycogen content and ameliorated insulin resistance were observed in the Se-LMWAP group. Overall, Se-LMWAP can be used as a promising dietary selenium supplement to exert -antidiabetic effect through modulating hepatic glucose metabolism and liver insulin-signaling transduction and oxidative stress.
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Current Research in Food Science
·
2024
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IF 6.2
Xiaolu Pu,Shuaipeng Yu,Yue Cui,Ziqian Tong,Changyan Wang,Lin Wang,Junhua Han,Hong Zhu,Shijie Wang
Two addition orders, i.e., the layer-by-layer (L) and mixed biopolymer (M) orders, were used to generate sodium caseinate - sugar beet pectin electrostatically stabilized o/w emulsions with 0.5% oil and varying sodium caseinate: sugar beet pectin ratios (3:1–1:3) at pH 4.5. Emulsion stability against environmental stresses (i.e., pH, salt addition, thermal treatment, storage and in vitro simulated gastrointestinal digestion) and its astaxanthin encapsulation against degradation during storage and in vitro digestion were evaluated. Results indicated that a total biopolymer concentration of 0.5% was optimal, with the preferred sodium caseinate-sugar beet pectin ratios for L and M emulsions being 1:1 and 1:3, respectively. L emulsions generally exhibited smaller droplet diameters than M emulsions across all ratios, except at 1:3. Lowering the pH to 1.5 substantially reduced the net negative charge of all emulsions, with only L emulsions precipitating at pH 3. M emulsions showed greater tolerance to salt addition, remaining stable up to 500 mM sodium and calcium concentrations, whereas L emulsions destabilized at levels exceeding 50 mM and 30 mM, respectively. All emulsions were stable when heated at 37 °C or 90 °C for 30 min. Astaxanthin degradation rates increased with prolonged storage, reaching 61.66% and 54.08% by day 7 for L and M emulsions, respectively. Encapsulation efficiency of astaxanthin in freshly prepared M emulsions (86.85%) was significantly higher compared to L emulsions (72.82%). M emulsions had 30% and 25% higher encapsulation efficiency of astaxanthin than L emulsions after in vitro digestion for 120 min and 240 min respectively. This study offers suggestions for interface design and process optimization to improve the performance of protein-polysaccharide emulsion systems, such as in beverages and dairy products, as well as their delivery effect of bioactives.
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高影响因子
CARBOHYDRATE POLYMERS
·
2025
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IF 12.5
Wei Zong,Zijing Liu,Haiyan Lan,Jianwei Yang,Yuxuan Xia,Zhongsheng Xu,Li Mai,Jie Wang,Yixi Bao
The polarization of tumor-associated macrophages (TAMs) from M2 to M1 phenotype is an effective strategy for tumor immunotherapy. In this study, a pectic polysaccharide (PEP-1) was isolated from Phyllanthus emblica fruits via hot water extraction, ethanol precipitation, and purified by anion exchange and gel permeation chromatography. PEP-1 was identified as a homogalacturonan (molecular weight, 156.8 kDa) via characterization analysis. The structural attributes of PEP-1 were elucidated through GC–MS and NMR analyses, confirming that its repeating sugar units consist of GalA connected by α-1,4-glycosidic linkage. Functional assays demonstrated that PEP-1, at concentrations of 50, 100, and 200 μg/mL could strongly induce the transformation of M2 macrophages into M1 phenotype in vitro, and the conditioned medium (CM) of M2 pretreated with PEP-1 significantly promoted apoptosis of Hepa1-6 cells. At the cellular level, PEP-1 shifted tumor-promoting M2 macrophages to a tumor-inhibiting M1 phenotype by increasing the phosphorylation of NF-κB and MAPK. Additionally, PEP-1 (200 mg/kg) was capable of reaching the tumor microenvironment (TME), directly binding to TAMs, promoting their polarization towards M1 phenotype, and significantly inhibiting the tumor growth in Hepa1-6 tumor-bearing mice. These findings reveal the structural characteristics of PEP-1 and its potential to treat hepatocellular carcinoma immunotherapy via regulating TAMs.
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FOOD RESEARCH INTERNATIONAL
·
2025
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IF 8
Wenwen Liu,Miao Li,Songyi Lin,Tongtong Shi,Yoshimasa Nakamura,Liang Song,Yue Tang
This study developed an emulsion delivery system using fish skin gelatin (FSG) and pectin (PEC) as complex emulsifiers to encapsulate benzyl isothiocyanate (BITC). The stability and BITC release profile of the FSG-PEC emulsion were evaluated in vitro and in vivo . Results indicated that the FSG-PEC emulsion significantly enhanced BITC bioavailability compared to water emulsion and single emulsifier systems, achieving targeted delivery. The BITC-loaded FSG-PEC emulsion significantly increased high-density lipoprotein cholesterol levels, reduced low-density lipoprotein cholesterol, cholesterol, and triglyceride levels, alleviated liver enzyme injuries, lowered creatinine and blood urea nitrogen levels, relieved oxidative stress induced by hyperglycemia, enhanced liver antioxidant capacity, reduced pro-inflammatory factor content in the liver, and improved liver tissue and islet damage in T2DM mice. This research not only provides a theoretical foundation for the delivery and controlled release of lipophilic active substances but also lays the groundwork for further exploration of their functional activities in vivo .
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Theranostics
·
2021
·
IF 11.556
Shi-Long Zhang,Yu-Qin Mao,Zheng-Yan Zhang,Zhan-Ming Li,Chao-Yue Kong,Hui-Ling Chen,Pei-Ran Cai,Bing Han,Tao Ye,Li-Shun Wang
Background: Anti-PD-1-based immunotherapy has emerged as a promising therapy for several cancers. However, it only benefits a small subset of colorectal cancer (CRC) patients. Mounting data supports the pivotal role of gut microbiota in shaping immune system. Pectin, a widely consumed soluble fiber, has been reported to ameliorate the imbalance of gut microbiota. Therefore, we aimed to explore the effect and the underlying mechanisms of pectin in improving anti-PD-1 mAb efficacy. Methods: The C57BL/6 mice were treated with a broad-spectrum antibiotic (ATB) cocktail to depleted endogenous gut microbiota and subsequently humanized with feces from healthy controls or newly diagnosed CRC patients. The antitumor efficacies of anti-PD-1 mAb combined with or without pectin were assessed using these mice. Flow cytometry and immunohistochemistry (IHC) were conducted to investigate the tumor immune microenvironment after treatment. The gut microbiota profiles and short-chain fatty acids (SCFAs) levels were determined by 16S ribosomal RNA (16S rRNA) gene sequencing and gas chromatography-mass spectrometry (GC-MS), respectively. The effect of gut microbiota on anti-PD-1 mAb efficacy after pectin supplement was further tested by fecal microbiota transplantation (FMT). Results: The anti-PD-1 mAb efficacy was largely impaired in the mice humanized with feces from newly diagnosed CRC patients compared to those from healthy controls. However, pectin significantly enhanced the anti-PD-1 mAb efficacy in the tumor-bearing mice humanized with CRC patient gut microbiota. Flow cytometry and IHC analysis revealed increased T cell infiltration and activation in the tumor microenvironment of mice treated with anti-PD-1 mAb plus pectin. In vivo depletion of CD8 + T cells diminished the anti-tumor effect of anti-PD-1 mAb combined with pectin. 16S rRNA gene sequencing showed that pectin significantly increased gut microbial diversity and beneficially regulated microbial composition. In addition, we identified unique bacterial modules that were significantly enriched in the anti-PD-1 mAb + pectin group, which composed of butyrate-producing bacteria indicative of good response to immunotherapy. Meanwhile, GC-MS showed that pectin altered the level of SCFA butyrate. Furthermore, butyrate, a main product of dietary fiber in gut microbial fermentation, was found to be sufficient to promote T cells infiltration and thus enhance the efficacy of anti-PD-1 mAb. In addition, FMT demonstrated the effects of pectin were dependent on gut microbiota. Importantly, the beneficial effects of pectin were confirmed in the mice humanized with gut microbiota from patient with resistance to anti-PD-1 mAb. Conclusion: Pectin facilitated the anti-PD-1 mAb efficacy in CRC via regulating the T cell infiltration in the tumor microenvironment, which was potentially mediated by the metabolite butyrate.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2020
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IF 4.972
Jian Gao,Chengzhi Liu,Jieyu Shi,Fangfang Ni,Qing Shen,Hujun Xie,Kuiwu Wang,Qunfang Lei,Wenjun Fang,Gerui Ren
The ovalbumin (OVA)-pectin (PEC)-sodium alginate (SA)-Vitamin D 3 (VD 3 ) complex nanoparticles were fabricated by antisolvent precipitation method, and the excellent encapsulation efficiency and loading capacity of VD 3 were obtained by 96.6% and 2.8%, respectively. Compared with ternary OVA-PEC-VD 3 complexes, the addition of SA with strong negative charge effectively regulated the OVA-PEC complexes and significantly improved the stability of OVA-PEC-SA-VD 3 complex nanoparticles with preferable size as small as 126 nm. The storage stability was also investigated after low temperature storage for 31 d, and the particle size of quaternary complexes was increased only 40 nm. In vitro digestion results elucidated that the complex nanoparticles had good stability in the simulated gastric fluid, and almost completely released in the simulated intestinal fluid confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) experiments and scanning electron microscope (SEM) images. The release kinetics study clarified that it was close to Fick release. Fluorescence and Fourier transform infrared spectroscopy (FTIR) experiments showed that quaternary complex nanoparticles were mainly combined by electrostatic, hydrophobic and hydrogen bonding interactions. The novel quaternary protein-polysaccharide complexes have excellent stability and great sustained-release performance for VD 3 , which may be helpful for the digestion and absorption of vitamin by human body, thus have potential applications in the food and drug industry.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Ru Zhao,Boxue Xia,Abbas Khan,Yanjiao Chang,Xin Guo,Yaxuan Liu,Xin Huang,Cuina Wang
Alkaline thermally treated pea protein and pectin complex was utilized to create high internal phase emulsions (HIPE) for extrusion-based 3D printing. Pea protein heated at pH 10 and 100 °C was optimized and chosen based on criteria such as solubility, surface hydrophobicity, particle size, and surface charge. The most promising ink for 3D printing was selected from HIPE stabilized by complexes containing 2 % heated pea protein with varying pectin concentrations (0.25 % ~ 1 %) and pH levels (3–7). The complex containing 1 % pectin at pH 7 endowed the emulsions with favorable rheological properties and particle size, as well as excellent heat resistance and storage stability. The optimized HIPE formulation was successfully used for 3D printing, resulting in printed shapes exhibiting robust self-supporting characteristics. Data indicated that the combination of alkaline thermally treated pea protein and pectin could serve as an excellent emulsifier for constructing HIPEs, suitable for applications in 3D printing within the food industry.
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高影响因子
Journal of Biomaterials and Tissue Engineering
·
2020
·Vol. 10
·期 1
·1-7
·
IF 0.511
Chen Linjing,Wang Fangfang,Luan Tao,Jiang Shuhong,Yu Feifei,Wang Ping
Medicinal care for cutaneous wound becomes difficult due to the emergence of antibiotic resistant bacteria. The development of a new cost-effective drug based on silver sulfadiazine loaded with pectin-zinc nanocomposites offers better therapeutics in wound healing besides its role in
enhancing antioxidant and antibacterial activities. In this study, silver sulfadiazine loaded with pectin-zinc nanocomposites (AgSD-PC-Zn) was produced and its antioxidant and antimicrobial properties were tested against wound infection causing bacterial pathogens (S. aureus and E.
coli). The synthesized AgSD-PC-Zn composite was biophysically characterized. TEM analysis confirmed the spherical shape of NPs. AgSD-PC-Zn composite exhibited a greater antibacterial effect against S. aureus and E. coli. The number of dead bacterial cells was higher after
exposure to AgSD-PC-Zn composite. Furthermore, in vitro antioxidant enzyme activities (DPPH and H2O2) were increased with AgSDPC-Zn composite. A rapid healing of the skin wounds in mouse was noticed with AgSD-PC-Zn composite treatment. This study concludes that
the newly formed AgSD-PC-Zn composite will act as a strong antimicrobial and antioxidant agents for treating skin wound infection.
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CHEMICAL ENGINEERING JOURNAL
·
2024
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IF 13.3
Tiantian Min,Jin Yue,Chuanxiang Cheng,Xiaoyun Ma,Shihan Weng,Yuwan Luo,Yujie Lei,Yangyang Long
Developing stimuli-responsive on-demand release systems is very important to effectively suppress microbial infections and excessive food decomposition. However, the commonly designed controlled release systems with a single responsiveness cannot accurately response to environmental changes in food packaging scenarios. Herein, a pH/enzyme dual-responsive active packaging was fabricated based on in situ growth of ZIF-8 nanoparticles (ZIF-8 NPs) on TEMPO-oxidized cellulose (TOCNF) film and loading with carvacrol (CAR), after that electrostatic adsorbing pectin as “gatekeeper” for fruit preservation. The developed CAR@ZIF-8/TOCNF/Pec film was able to respond to acidic condition and pectinase produced by microorganisms in the case of fungal infections during food storage, thus possessing a dual-responsive controlled-release function. The release rate of CAR reached approximately 86.83 ± 1.82 % at pH = 5.0 and reached 88.65 ± 1.86 % in the presence of pectinase (1.0 mg/mL) after 168 h. In addition, the CAR@ZIF-8/TOCNF/Pec film performed superior inhibition efficacy against Escherichia coli, Staphylococcus aureus , and Aspergillus niger . The CAR@ZIF-8/TOCNF/Pec film were highly effective in preserving the freshness of fungus-inoculated raspberries and mangoes, and inhibited the spread of fungal spores during storage for 5 and 7 days, respectively. Therefore, this work provided a promising, smart and eco-friendly strategy for on-demand and precise release of bioactive ingredients during food storage and preservation.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Youchuan Ma,Jinfeng Bi,Zhonghua Wu,Jianyong Yi
Saccharides are the dominant factor shaping the texture of freeze-dried products. This study investigated the impact of various molecular-weight saccharides at different concentrations on the physical properties and intermolecular interactions of pectin-CMC cryogels by experiment and molecular dynamic (MD) simulations. Results showed that the increased shrinkage of cryogels and enhanced molecular interactions between saccharides and pectin-CMC were mechanisms that enhanced the hardness of cryogels. Notably, the cryogel with fructose exhibited significantly higher hardness compared to cryogels with other saccharides. The cryogel containing 16 % fructose had the lowest glass transition temperature ( T g ), leading to the highest shrinkage (70 %). Moreover, the MD results revealed that fructose had the highest intermolecular interaction energy (coulombic short range) with pectin-CMC (−5316.78 kJ/mol). This study revealed that the intermolecular interactions between the added saccharide and the pectin-CMC, in addition to the density of the cryogel, are critical determinants of the hardness and crispness of cryogels.
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Food Bioscience
·
2026
·
IF 6.2
Yue Meng,Fangfang Su,Yuhan Cui,Hanying Yang,Qian Guo,Wei Zhang,Ling Guo,Yujun Jiang,Chaoxin Man,Yu Zhang
Probiotics have shown potential in alleviating ulcerative colitis (UC) by modulating gut microbiota, restoring intestinal barrier integrity, and suppressing inflammatory responses. However, their oral efficacy is limited by poor gastrointestinal survival and insufficient colonic targeting. To address these limitations, a ternary hydrogel microcapsule (JYPWH) composed of apple pectin, whey protein isolate, and hyaluronic acid (HA) was developed to encapsulate Lacticaseibacillus rhamnosus JY027. As a dual-functional delivery system, JYPWH combined probiotic protection against gastrointestinal stress with HA-CD44-mediated delivery to inflamed colonic tissue. Oral administration of JYPWH significantly alleviated DSS-induced colitis in mice. Mechanistically, JYPWH promoted intestinal barrier repair by upregulating MUC-2, ZO-1, and Occludin. Concurrently, the NF-κB signaling pathway was suppressed in colonic tissue, serum levels of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were decreased, and serum levels of the anti-inflammatory cytokine IL-10 were increased. JYPWH also reduced inflammation-induced CD44 overexpression in colonic tissue. In addition, JYPWH reshaped the gut microbiota of DSS-treated mice by increasing beneficial taxa such as Lachnospiraceae_NK4A136_group and decreasing opportunistic pathogens, including Escherichia-Shigella , while restoring colonic short-chain fatty acid levels. Collectively, JYPWH integrates probiotic protection with HA-CD44-mediated colonic delivery, offering a promising strategy for UC intervention.
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危险品化学品经营许可证（带存储）