JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 11
·117508
·
IF 4.386
Jinliang Chen,Sha Han,Hongxia Li,Xiaohui Niu,Kunjie Wang
Nitrogen-doped carbon quantum dots (N-CQDs) were synthesized by one-step hydrothermal method using citric acid as carbon source and urea as nitrogen source. β-cyclodextrin (β-CD) is a cyclic oligosaccharide with a unique hollow structure, which is beneficial to the enrichment of metal ions on the electrode surface while reducing the agglomeration of carbon quantum dots and maintaining conductivity. When Cu2+ is coordinated with β-CD, it can further increase the affinity for Cu2+ and recognize Cu2+ more sensitively. Therefore, we coordinated Cu2+ and β-CD to form copper ion coordinated β-cyclodextrin (Cu-β-CD). By self-assembly, the prepared N-CQDs and Cu-β-CD combined to form Cu-β-CD/N-CQDs composites. The electrochemical sensor constructed by Cu-β-Cd/N-CQDs successfully realizes the selective detection of Cu2+ in the range of 1–125 μmol l−1, and the lowest detection limit is 0.094 μmol l−1. In the detection of actual water samples, the Recovery of Cu2+ was between 92% and 120%, and the RSD was between 2.3% and 5.2%. Therefore, Cu-β-CD/N-CQDs composite is expected to be constructed as a non-toxic, pollution-free electrochemical sensor for selective detection of heavy metal Cu2+ ions in water.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Xuejiao Hu,Guizhou Xu,Huaicheng Zhang,Meng Li,Yizhou Tu,Xianchuan Xie,Yuanting Zhu,Lu Jiang,Xingqi Zhu,Xiaowen Ji,Yan Li,Aimin Li
Natural organic matter (NOM), organic micropollutants (OMPs), and detrimental microorganisms are three major pollutants that affect water quality. To remove these pollutants, a quaternary ammonium-functionalized β-cyclodextrin polymer (β-CDP) is successfully synthesized in the aqueous phase. The N2 and CO2 adsorption/desorption analysis showed that the polymer mainly contains ultra-micropores (<1 nm), with a Langmuir surface area of 89 m2 g–1. Two kinds of NOM, humic acid and fulvic acid, and five OMPs, 2-naphthol (2-NO), 3-phenylphenol (3-PH), 2,4,6-trichlorophenol (2,4,6-TCP), bisphenol A (BPA), and bisphenol S (BPS), were selected as model pollutants to study the performance of β-CDP and three kinds of commercial adsorbents, including granular activated carbon, DARCO-AC, and two resins, XAD-4 and D-201, were used for comparison. The polymer shows ultrarapid adsorption kinetics for the removal of these pollutants, with pseudo-second-order rate constants two to three orders of magnitude higher than that of the commercial activated carbon and resins. Due to the different adsorption sites of NOM and OMPs, β-CDP can simultaneously remove these pollutants without competitive adsorption. The maximum adsorption capacity of β-CDP for HA, FA, 2-NO, 3-PH, 2,4,6-TCP, BPA, and BPS based on the Langmuir model is 40, 166, 74, 101, 108, 103, and 117 mg g–1, respectively. After use, the polymer can be easily regenerated at room temperature. In addition, β-CDP also showed excellent bactericidal properties due to the quaternary ammonium groups. At a concentration of 15 g L–1, β-CDP can remove 98% of the tested Escherichia coli. Moreover, the synthesis of β-CDP is simple, green, and easy to industrialize. All of these findings indicate that β-CDP, as an ideal multifunctional material, presents potential for practical applications for water treatment and disinfection.
研究领域:
环境科学
·
材料科学
·
水处理技术
✓ 已验证
DOI
PubMed
高影响因子
CELL BIOLOGY INTERNATIONAL
·
2023
·
IF 3.9
Hongyang Xie,Junyu Chen,Changyan Ma,Jingjing Zhao,Manhua Cui
Dysregulation of the deubiquitinating protease, UBP43, has been implicated in many human diseases, including cancer. Here, we evaluated the functional significance and mechanism of action of UBP43 in epithelial ovarian cancer. We found that UBP43 was significantly upregulated in the tumor tissues of patients with epithelial ovarian cancer. Similar results were observed in OVCAR-3, Caov-3, TOV-112D, A2780, and SK-OV-3 cells. Furthermore, in vitro functional assays of A2780 and TOV-112D cells demonstrated that UBP43 overexpression promoted cell proliferation, migration, and invasion. Upregulation of UBP43 might result in epithelial–mesenchymal transition by inducing the nuclear transport of β-catenin, which was accompanied by enhanced N-cadherin but decreased E-cadherin expression. These malignant phenotypes were reversed by UBP43 silencing. Further investigation revealed that the knockdown of UBP43 inhibited cell proliferation by inducing a cell cycle arrest at the G2/M phase. The oncogenic characteristics of UBP43 were validated in a subcutaneous xenograft mouse model. In vivo, tumor growth was delayed in the UBP43-silenced group but accelerated after UBP43 overexpression. Finally, we demonstrated that β-catenin is a key protein in the UBP43-mediated malignant development of epithelial ovarian cancer. Specifically, overexpression of UBP43 decreased the ubiquitination degradation of β-catenin and enhanced its protein stability. Also, we observed that the downstream genes of beta-catenin such as cyclin D1, MMP2, and MMP9 were upregulated due to UBP43 overexpression. Thus, we concluded that UBP43 promoted epithelial ovarian cancer tumorigenesis and metastasis through activation of the β-catenin pathway, suggesting that UBP43 may be a potential therapeutic target for this intractable disease.
研究领域:
肿瘤学
·
细胞生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
TETRAHEDRON
·
2019
·
IF 2.379
Jingyuan Li,Qingwen Song,Hengxuan Zhang,Ping Liu,Kan Zhang,Junwen Wang,Dengsong Zhang
Chemical fixation of carbon dioxide under mild reaction conditions e.g. atmospheric pressure and low temperature depends upon the ability of catalyst. Herein, a synergistic catalytic scheme of silver sulfadiazine/ n Bu 4 NBr was described for the three-component reaction of propargylic alcohols, CO 2 , and monohydric alcohols. This catalytic system was demonstrated effectively to provide β-oxopropyl carbonates in excellent yields (up to 99% yield with 5 mol% catalyst). The method tolerated a wide scope of propargylic alcohols and monohydric alcohols under atmospheric CO 2 pressure and solvent-free conditions. The excellent catalytic performance was attributed to the synergistic catalysis confirmed by the careful experiments.
研究领域:
催化
·
有机化学
·
绿色化学
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2018
·
IF 2.533
Qi Chen,Shaofei Zhang,Yunchang Xie
The enzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature of β-carboline (βC) alkaloid biosynthesis. NscbB is a rare microbial PSase discovered from a cryptic β-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplant patient derived) by homologous alignment with its well-characterized counterpart McbB. The biochemical analysis showed that NscbB could catalyze l -tryptophan ( K M = 89.64 ± 8.69 μM) and methylglyoxal ( K M = 147.70 ± 16.38 μM), without cofactors, to form the two βC skeletons 1-acetyl-3-carboxy-β-carboline and 1-acetyl-β-carboline in vitro . Additionally, the heterologous expression of nscbB in E. coli BL21 (DE3) revealed the efficient bioproductivity of NscbB to bioproduce two βC skeletons, 1-acetyl-3-carboxy-β-carboline (5.5 mg/L) and 1-acetyl-β-carboline (3.1 mg/L), within 16 h fermentation. These results demonstrate NscbB is a novel and practical microbial PSase, which is useful for future bioactive βC alkaloid exploitation and development.
研究领域:
生物化学
·
微生物学
·
生物技术
·
酶学
✓ 已验证
DOI
PubMed
高影响因子
AAPS PHARMSCITECH
·
2021
·Vol. 22
·期 7
·1-7
·
IF 3.246
Wang Beilei,Shan Xiaoxiao,Lv Shujie,Zha Liqiong,Zhang Caiyun,Dong Qiannian,Chen Weidong
3-O-β-D-galactosylated resveratrol (Gal-Res) was synthesized from resveratrol (Res) and 3-O-β-D-galactose (Gal) in our previous study. In order to improve the pH sensitivity and bioavailability of Gal-Res, Gal-Res nanoparticles (Gal-Res NPs) were prepared using polydopamine (PDA) as a drug carrier. The drug loading (DL %) and entrapment efficiency (EE %) of Gal-Res NPs were 46.80% and 88.06%. The average particle size, polydispersity index (PDI), and Zeta potential of Gal-Res NPs were 179.38 ± 2.83 nm, 0.129 ± 0.013, and − 28.05 ± 0.36 mV, respectively. The transmission electron microscope (TEM) showed that Gal-Res NPs had uniform spherical morphology. Compared with the fast release of raw Gal-Res, the in vitro release of Gal-Res NPs was slow and pH-sensitive. The results of the blood vessel irritation and hemolysis test demonstrated that Gal-Res NPs had good hemocompatibility. The pharmacokinetics study in rats showed that area under the curve of plasma drug concentration time (AUC 0→600 ) and half-life (t 1/2 ) of Gal-Res NPs were enhanced 1.82-fold and 2.19-fold higher than those of raw Gal-Res. The in vivo biodistribution results showed that Gal-Res NPs were more distributed in liver tissue than Gal-Res. Gal-Res NPs with high bioavailability and liver accumulation were hopeful drug delivery systems (DDS) to treat liver diseases. Graphical abstract
研究领域:
药剂学
·
生物医学工程
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Xiuhong Wu,Boquan Qu,Yongkang Liu,Xingxuan Ren,Shaoyan Wang,Yanling Quan
In this study, an enzyme immobilization method for the effective biotransformation of ginsenoside Rb1 to impart activity and stability was developed. Using a hydrolase enzyme model, β-glucosidase from Aspergillus niger , immobilization within chemically affinity-linked amino-based silica provided an immobilization efficiency 5.86-fold higher than that of free enzyme. Compared with the free enzyme, the immobilized enzyme functioned optimally at a wider pH range and had higher thermostability. The optimum pH for the free and immobilized enzymes was 5.5. The optimal reaction temperature of the immobilized enzyme was 45 °C, which was 5 °C higher than that of the free enzyme. The Michaelis constant ( K m ) values before and after immobilization were 0.482 mmol•L −1 and 0.387 mmol•L −1 , respectively. The catalytic rate ( K cat ) for the immobilized and free enzymes was 22.269 mmol•L −1 and 8.800 mmol•L −1 , respectively, and the catalytic efficiency ( K cat / K m ) activity of the immobilized enzyme was 3.30-fold higher than that of the free enzyme. The immobilized enzyme could preserve 97 % of the activity after 45 cycles of repeated use. The high catalytic activity and significant operational stability are beneficial for industrial applications.
研究领域:
生物化学
·
酶工程
✓ 已验证
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2014
·
IF 6.007
Ruiping Hu,Xin Xiao,Shunheng Tu,Xiaoxi Zuo,Junmin Nan
Novel flower-like heterostructured β-Bi 2 O 3 /Bi 2 O 2 CO 3 microspheres are synthesized by calcining a Bi 2 O 2 CO 3 self-sacrifice precursor for the visible-light photocatalytic degradation of o -phenylphenol (OPP, is a widely used fungicide and preservative agent). The Bi 2 O 2 CO 3 microspheres are firstly prepared under hydrothermal conditions, and then converted to Bi 2 O 3 by thermal treatment. With increasing the calcining temperature from 250 to 500 °C, an in situ stepwise decomposition reaction take place during the course of calcination , described as: Bi 2 O 2 CO 3 → β-Bi 2 O 3 /Bi 2 O 2 CO 3 → β-Bi 2 O 3 → β-Bi 2 O 3 /α-Bi 2 O 3 → α-Bi 2 O 3 . The β-Bi 2 O 3 /Bi 2 O 2 CO 3 microspheres synthesized at 300 °C exhibit excellent photocatalytic activity under visible-light irradiation, which can degrade 99.8% OPP in 45 min. And the degradation rate of the heterostructured photocatalyst is approximately 2, 2.6, 6, 13, 80, and 827 times higher than that of single β-Bi 2 O 3 , mixed β-Bi 2 O 3 and Bi 2 O 2 CO 3 , commercial β-Bi 2 O 3 , α-Bi 2 O 3 , N-doped TiO 2 , and Bi 2 O 2 CO 3 , respectively. The superior photoreactivity of the β-Bi 2 O 3 /Bi 2 O 2 CO 3 is attributed to the enhanced charge separation and transfer due to the formation of p–n junction with large heterojunction interface, favorable band gap energy (2.27 eV), relatively high specific surface areas (12.5 m 2 g −1 ), and flower-like hierarchical micro/nano structures. In addition, the degradation intermediates including ethyl phenethyl ether, phenyl acetaldehyde, and phenylacetic acid are identified. And the results also reveal that the photogenerated holes and •O 2 − radicals are primarily reactive species in the photocatalytic system, which are the key factors responsible for the nearly complete mineralization of OPP.
研究领域:
材料科学
·
环境科学
·
光催化
✓ 已验证
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 11
·1847-1855
·
IF 3.614
Ben Chen,Haibo You,Liqun Fang,Tingting Lin,Ping Xu,Chu Chu,Shengqiang Tong
Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans -paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive. Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied. Apparent formation constant between methanol, acetonitrile, and sulfobutylether-β-cyclodextrin were determined to be 2.90 × 10 −3 and 1.00 × 10 −4 L mmol −1 under 25°C using UV-spectrophotometry. Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers–stationary phase interaction and formation of inclusion complex. Two retention models were employed individually for evaluation of inclusion complexation between five racemates and sulfobutylether-β-cyclodextrin. The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans -paroxol and atropine sulfate. In the selected mobile phase, stoichiometric ratio for both of inclusion complex was found to be 1:1.
研究领域:
药学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Ling Chen,Ming Li,Youhong Ai,Xueping Dang,Jianlin Huang,Huaixia Chen
This work describes, for the first time, an acryloyled β -cyclodextrin hybrid monolith column was synthesized, under aqueous-phase conditions, and used for solid-phase microextraction of carbendazim and carbaryl . The monolithic column was characterized using scanning electron microscopy, nitrogen adsorption–desorption, thermogravimetric analysis and Fourier transform infrared spectroscopy, and used as the adsorbent for solid phase microextraction (SPME) of carbendazim and carbaryl. After optimization of the SPME conditions, a simple and sensitive SPME-HPLC method was developed for the determination of carbendazim and carbaryl in leafy vegetables. The method exhibited a good liner response in the range 5–400 μg/kg (R 2 = 0.9994) for carbendazim and 10–400 μg/kg (R 2 = 0.9996) for carbaryl, respectively. The limits of detection were 1.0 and 1.5 μg/kg for carbendazim and carbaryl, respectively, in leafy vegetables. Recoveries ranged from 92.6% to 110.1%, and the relative standard deviations were less than 6.1%.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
DOI
高影响因子
Pharmaceutics
·
2020
·Vol. 12
·期 2
·111
·
IF 4.421
Weinan Li,Jialin Sun,Xiaoyu Zhang,Li Jia,Mingxi Qiao,Xiuli Zhao,Haiyang Hu,Dawei Chen,Yanhong Wang
PEG-poly(β-amino ester) (PEG-PBAE), which is an effective pH-responsive copolymer, was mainly synthesized by Michael step polymerization. Thioridazine (Thz), which was reported to selectively eliminate cancer stem cells (CSCs), was loaded into PEG-PBAE micelles (PPM) prepared by self-assembly at low concentrations. The critical micelle concentrations (CMC) of PPM in water were 2.49 μg/mL. The pH-responsive PBAE segment was soluble due to protonated tertiary amine groups when the pH decreased below pH 6.8, but it was insoluble at pH 7.4. The Thz-loaded PEG-PBAE micelle (Thz/PPM) exhibited a spherical shape, and the drug loading was 15.5%. In vitro release of Thz/PPM showed that this pH-sensitivity triggered the rapid release of encapsulated Thz in a weakly acidic environment. The in vitro cytotoxicity and cellular uptake of various formulations at pH 7.4 and 5.5 were evaluated on the mammospheres (MS), which were sorted by MCF-7 human breast cancer cell lines and identified to be a CD44+/CD24−phenotype. The results of the cytotoxicity assay showed that blank micelles were nontoxic and Thz/PPM exhibited a similar anti-CSC effect on MS compared to Thz solution. Stronger fluorescence signal of Coumarin-6 (C6) was observed in MS treated by C6-loaded PPM (C6/PPM) at pH 5.5. The tumor inhibition rate and tumor weight of the free DOX and Thz/PPM groups were significantly different from those of the other groups, which free DOX and Thz/PPM effectively suppressed breast tumor growth in vivo. The above experimental results showed that Thz/PPM is an ideal and effective pH-responsive drug delivery carrier to a targeted therapy of CSCs.Keywords:poly(β-amino ester);polymeric micelles;pH-responsive;cancer stem cells;thioridazine
研究领域:
药学
·
肿瘤学
·
生物材料学
✓ 已验证
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Yanxin Xiang,Jiali Zhang,Huanv Mao,Zexin Yan,Xuebin Wang,Chunyan Bao,Linyong Zhu
Peptide-based supramolecular hydrogels have attracted great attention due to their good biocompatibility and biodegradability and have become promising candidates for biomedical applications. The bottom-up self-assembly endows the peptides with a highly ordered secondary structure, which has proven to be an effective strategy to improve the mechanical properties of hydrogels through strong physical interactions and energy dissipation. Inspired by the excellent mechanical properties of spider-silk, which can be attributed to the rich β-sheet crystal formation by the hydrophobic peptide fragment, a hydrophobic peptide (HP) that can form a β-sheet assembly was designed and introduced into a poly(vinyl alcohol) (PVA) scaffold to improve mechanical properties of hydrogels by the cooperative intermolecular physical interactions. Compared with hydrogels without peptide grafting (P-HP0), the strong β-sheet self-assembly domain endows the hybrid hydrogels (P-HP20, P-HP29, and P-HP37) with high strength and toughness. The fracture tensile strength increased from 0.3 to 2.1 MPa (7 times), the toughness increased from 0.4 to 21.6 MJ m–3 (54 times), and the compressive strength increased from 0.33 to 10.43 MPa (31 times) at 75% strain. Moreover, the hybrid hydrogels are enzymatically degradable due to the dominant contribution of the β-sheet assembly for network cross-linking. Combining the good biocompatibility and sustained drug release of the constructed hydrogels, this hydrophobic β-sheet peptide represents a promising candidate for the rational design of hydrogels for biomedical applications.
研究领域:
材料科学
·
生物材料
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）