Biomolecules
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2019
·Vol. 9
·期 7
·294
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IF 4.694
Yaling Tian,Peihong Deng,Yiyong Wu,Ziyu Ding,Guangli Li,Jun Liu,Quanguo He
In this paper, a tryptophan (Trp) molecularly imprinted chitosan film was prepared on the surface of an acetylene black paste electrode using chitosan as the functional polymer, Trp as the template molecule and sulfuric acid as the crosslinking agent. The surface morphologies of non-imprinted and imprinted electrodes were characterized by scanning electron microscopy (SEM). The formation of hydrogen bonds between the functional polymer and the template molecule was confirmed by infrared spectroscopy. Some factors affecting the performance of the imprinted electrode such as the concentration of chitosan, the mass ratio of chitosan to Trp, the dropping amount of the chitosan-Trp mixture, the solution pH, and the accumulation potential and time were discussed. The experimental results show that the imprinted electrode exhibit good affinity and selectivity for Trp. The dynamic linear ranges of 0.01–4 μM, 4–20 μM and 20–100 μM were obtained by second derivative linear sweep voltammetry, and the detection limit was calculated to be 8.0 nM. The use of the imprinted electrode provides an effective method for eliminating the interference of potentially interfering substances. In addition, the sensor has high sensitivity, reproducibility and stability, and can be used for the determination of Trp in pharmaceutical preparations and human serum samples.Keywords:molecularly imprinted chitosan film;electrochemical sensor;tryptophan;selective determination
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JOURNAL OF POWER SOURCES
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2019
·
IF 7.467
Bin Wang,Xin-Ye Lin,Yuanyuan Tang,Qiang Wang,Michael K.H. Leung,Xiao-Ying Lu
With the massive consumption of lithium-ion batteries in portable consumer electronics and electric vehicles, proper disposal of spent batteries is of paramount importance for sustainable development. In this study, biodegradable organic methanesulfonic acid (MSA) is investigated for the first time to leach valuable metals from waste LiCoO 2 powders for battery material regeneration. Under the optimal conditions, leaching efficiencies of lithium and cobalt are achieved at nearly ∼100% and ∼100%, respectively. Comparison experiment indicates that MSA can achieve better leaching performance than previously reported organic acids (e.g. citric acid , malonic acid , succinic acid , oxalic acid) under the same conditions. Besides, strong leaching capability of MSA at high solid-to-liquid ratios (e.g. 81.2 g L −1 and 163 g L −1 ) is confirmed with satisfactory leaching efficiencies (ca. 92.4%–100%). Feasibility test of MSA utilization for proper treatment of spent batteries is demonstrated at the optimized conditions. Importantly, regeneration study suggests that the as-obtained leachate can be employed to synthesize Co 3 O 4 anode and LiCoO 2 cathode materials with micro/nanostructures. Excellent cycling performance and rate capability of the regenerated electrode materials are demonstrated in the repeated charge-discharge cycles. Overall, the proposed recycling strategy is of great significance for rational resource utilization of spent lithium-ion batteries.
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BMC GENOMICS
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2017
·Vol. 18
·期 1
·1-15
·
IF 3.729
Lu Yuzhen,Luo Feifei,Cen Kai,Xiao Guohua,Yin Ying,Li Chunru,Li Zengzhi,Zhan Shuai,Zhang Huizhan,Wang Chengshu
Background Ascomycete Cordyceps species have been using as valued traditional Chinese medicines. Particularly, the fruiting bodies of Cordyceps cicadae (syn. Isaria cicadae ) have long been utilized for the treatment of chronic kidney disease. However, the genetics and bioactive chemicals in this fungus have been largely unexplored. Results In this study, we performed comprehensive omics analyses of C. cicadae , and found that, in contrast to other Cordyceps fungi, C. cicadae produces asexual fruiting bodies with the production of conidial spores instead of the meiotic ascospores. Genome sequencing and comparative genomic analysis indicate that the protein families encoded by C. cicadae are typical of entomopathogenic fungi, including the expansion of proteases and chitinases for targeting insect hosts. Interestingly, we found that the MAT1-2 mating-type locus of the sequenced strain contains an abnormally truncated MAT1-1-1 gene. Gene deletions revealed that asexual fruiting of C. cicadae is independent of the MAT locus control. RNA-seq transcriptome data also indicate that, compared to growth in a liquid culture, the putative genes involved in mating and meiosis processes were not up-regulated during fungal fruiting, further supporting asexual reproduction in this fungus. The genome of C. cicadae encodes an array of conservative and divergent gene clusters for secondary metabolisms. Based on our analysis, the production of known carcinogenic metabolites by this fungus could be potentially precluded. However, the confirmed production of oosporein raises health concerns about the frequent consumption of fungal fruiting bodies. Conclusions The results of this study expand our knowledge of fungal genetics that asexual fruiting can occur independent of the MAT locus control. The obtained genomic and metabolomic data will benefit future investigations of this fungus for medicinal uses.
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CHEMISTRY OF MATERIALS
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2017
·
IF 9.466
Hao Wu,Zhimin Chen,Jialin Zhang,Feng Wu,Chunying He,Zhiyu Ren,Yiqun Wu
Manipulating the morphology and protonic acid doping of polyaniline (PANI) is significant for optimizing its NH3-sensing. Herein, tetra-β-carboxyphthalocyanine cobalt(II) (TcPcCo) acted as the dopant and structure-directing agent simultaneously to fabricate the uniform fibrous network-like PANI (PANI-TcPcCo hybrids) by a one-step polymerization at low temperature. During the reaction process, the protonic acid groups in TcPcCo not only induced the aniline monomers polymerizing into one-dimensional nanofibers (consist of both solid and hollow cylinders) with abundant tiny protuberances on the surface but also successfully doped into PANI. The resulting PANI-TcPcCo hybrids displayed the enhancement in terms of the good conductivity, the large gas adsorption capacity, and the unobstructed channels for the electron and gas transport. The central metal atoms of TcPcCo present the strong and selective affinity to NH3. Meanwhile, the deep-seated conversion of PANI’s molecular structure after exposure in NH3 could occur due to the presence of TcPcCo. Thus, the PANI-2.5TcPcCo sensor showed the excellent NH3-sensing performance at room temperature, including an ultrahigh and fast response (802.7% and ∼17.0 s for 100 ppm of NH3), a very low detection limit of 10 ppb (about 5000 parts of human olfaction limit of detection, 55 ppm), and superior NH3-sensing stability and selectivity. The strategy developed here provides a reliable and valid way to synthesize functional PANI-based hybrids with unique morphology and appropriate doping, which are able to be extended to other areas.
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Food Bioscience
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2024
·
IF 4.8
Xinyue Qin,Tao Xiong,Xinyi Kang,Zhanggen Liu,Mingyong Xie,Qianqian Guan
Suansun (fermented bamboo shoots) was prepared by natural fermentation (NF) and inoculation fermentation (IF) with a starter culture containing Lactococcus lactis , Leuconostoc citreum , and Lactiplantibacillus plantarum in this study, to evaluate the effects of inoculation mixed starter culture on physicochemical, flavor and sensory characteristics of suansun. The results showed that IF suansun acidified faster and to a greater extent than NF. Besides, the accelerated acidification process significantly decreased the nitrite content, shortened the fermentation time and improved the food safety of the final suansun product in IF. At the end of fermentation, the lactic acid concentration was significantly higher in IF (6.17 ± 0.25 g/L) than in NF (3.83 ± 0.28 g/L). Twenty-seven volatile compounds were identified as the characteristic flavor compounds in suansun and the relative content of nonanal, hexanal, 1-octen-3-ol and 3-octanone (ROAV >1) was higher in IF than in NF at the late stage of fermentation. In addition, 132 non-volatile compounds were identified as significant differential metabolites, which were mainly small peptides and fatty acids in both NF and IF. Sensory evaluation found that the aroma, taste, texture and overall quality scores of IF suansun were all superior to those of NF suansun. The above results demonstrated that the use of mixed starter cultures of Lac. lactis , Leu. citreum , and L. plantarum contributed to improving the quality of suansun product, and might provide a feasible approach for standardized production of suansun in the future.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2024
·
IF 8.1
Zhiyan Sui,Zhe Wang,Yan Wang
Using carboxylated graphene oxide (CFGO) as inorganic crosslinking agent, a series of poly (2,6-dimethyl-1,4-phenylene oxide) (PPO) based complex anion exchange membranes (AEMs) with pyridine cations as conduction sites were prepared successfully. The carboxyl modification can improve the dispersibility of inorganic filler in the polymer and provide more crosslinking sites. At the same time, the two-dimensional layered structure of CFGO and a large number of hydrophilic groups provide good ion transport channels. As a result, compared with the original membrane, the crosslinked composite membranes showed better conductivity and chemical stability. Among them, the conductivity of AP-PPO-CFGO2 reached 102.41 mS/cm at 80 °C, and retained about 70% of the conductivity after soaking in 2 M NaOH for 800 h. Furthermore, the AP-PPO-CFGO2 displayed a power density of 277.5 mW/cm 2 in a H 2 /O 2 single cell test at 80 °C.
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Ke Wang,Wei Gao
Efficient thermal insulation and flame-retardant materials are crucial in reducing energy consumption and minimizing fire hazards. This work presents a novel method for preparing a composite aerogel: a silane coupling agent was used as a chemical bridge to synthesize a new flame retardant AKH (modified APP with HNTs). AKH was incorporated into the silica sol to fabricate the SA-AKH composite aerogel. SA-AKH features a dense three-dimensional network skeletal structure, excellent thermal stability, and a high specific surface area. When integrated into organosilicon resin, SA-AKH significantly enhances the flame resistance of composite materials. During cone calorimetry testing (CCT), SSAKH4 exhibited a peak heat release rate (PHRR) of 281.88 kW/m 2 and total smoke production (TSP) of 5.52 m 2 , representing a decrease of 30.8 % and 4.5 %, respectively, compared to SSA4. The composite material, derived from the unique mesoporous structure of aerogel combined with the flame retardant and hollow characterization of AKH, demonstrates exceptional flame retardancy and smoke suppression. SA-AKH is expected to make novel advancements in the field of high-efficiency insulation and flame-resistant composites.
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Journal of Materials Chemistry A
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2024
·
IF 10.7
Zi-Ye Liu,Qian-Yu Wang,Teng Xu,Ji-Ming Hu
Nickel–iron layered double hydroxide (NiFe LDH) shows great performance in the oxygen evolution reaction (OER) due to the interaction between Ni and Fe. However, such an interaction still remains insufficient to achieve lower overpotential. To address this, we employ conjugated polycarboxylate ligands to promote electron transfer between Ni and Fe in NiFe LDH. This results in the formation of high-valence Ni in NiFe LDH, and also strengthens the adsorption of O intermediates at Ni sites. In this study, oxalate and 1,3,5-benzenetricarboxylate (BTC) are used to modify NiFe LDH, synthesized via the gas–solid reaction method and the decomposition of MIL-100(Fe) method, respectively. Oxalate-modified NiFe LDH has a low overpotential of 238 mV and Tafel slope of 38.1 mV dec−1, while BTC-modified NiFe LDH is 230 mV and 36.1 mV dec−1. In addition, both of them can work stably for over 24 h at a current density of 100 mA cm−2. The conjugated polycarboxylate ligand modification method is a new strategy to promote the OER performance and extend long-term stability of NiFe LDH.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Qiaolong Zhai,Jie Li,Kangze Miao,Yunhao Song,Shaoqi Yang,Shuheng Zhao,Yanju Lu,Jianjun Hu
This study developed a “one-pot” three-stage process using a “multifunctional” deep eutectic solvent (DES) containing choline chloride (ChCl), ethylene glycol (EG), and protonic acids for the production of phenolic monomers, furfural, and glucose. In the first stage, the DES effectively dissolved over 70 % of lignin and 78 % of hemicellulose while preserving aryl ether bonds in lignin due to the grafting of EG onto the aryl ether bonds. Concurrently, the retention of a near-quantitative amount of cellulose led to a glucose yield of >80 % after enzymatic saccharification. In the next stage, the DES enabled the catalytic depolymerization of lignin using a Ru/C catalyst at mild temperatures and atmospheric pressure, eliminating the need for an external hydrogen source and yielding G/S-propyl and G/S-propenyl monomers at 13.8 %. Additionally, the ratio of ChCl to EG in the DES could regulate the composition and selectivity of the phenolic monomers. Following this, the hemicellulose sugars dissolved in the DES underwent catalytic hydrolysis in a DES/water system, achieving a furfural yield of 36.4 % under optimized conditions. The results of this study offer important insights into the valorization of lignocellulose in “one-pot” under mild conditions, thereby advancing the field of biorefining.
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ACS Nano
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2024
·
IF 15.8
Shuting Yang,Bing Qi,Mingzi Sun,Wenjing Dai,Ziyun Miao,Wei Zheng,Bolong Huang,Jie Wang
Persistent luminescence describes the phenomenon whereby luminescence remains after the stoppage of excitation. Recently, upconversion persistent luminescence (UCPL) phosphors that can be directly charged by near-infrared (NIR) light have gained considerable attention due to their promising applications ranging from photonics to biomedicine. However, current lanthanide-based UCPL phosphors show small absorption cross sections and low upconversion charging efficiency. The development of UCPL phosphors faces challenges due to the lack of flexible upconversion charging pathways and poor design flexibility. Herein, we discovered a lattice defect-mediated broadband photon upconversion process and the accompanying NIR-to-NIR UCPL in Cr-doped zinc gallate nanoparticles. The zinc gallate nanoparticles can be directly activated by broadband NIR light in the 700–1000 nm range to produce persistent luminescence at about 700 nm, which is also readily enhanced by rationally tailoring the lattice defects in the phosphors. This proposed UCPL phosphor achieved a signal-to-background ratio of over 200 in bioimaging by efficiently avoiding interference from autofluorescence and light scattering. Our work reported a lattice defect-mediated photon upconversion phenomenon, which significantly expands the horizons for the flexible design of UCPL phosphors toward broad applications ranging from bioimaging to photocatalysis.
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JOURNAL OF NANOBIOTECHNOLOGY
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2024
·Vol. 22
·期 1
·1-16
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IF 10.6
Li Qian,Liu Chenguang,Xing Dongming
On-demand dissolution of hydrogels has shown much potential in easy and pain-free removal of wound dressings. This work firstly describes a type of carbon dots (CDs) for dissolving Ca-alginate hydrogel via site-specific mineralization method. The CDs were characterized by two features, which included presence of primary/secondary amine groups and generation of calcium crystals with Ca 2+ . Especially, the amount of primary/secondary amine groups on CDs played key role in determining whether hydrogel could be dissolved. When there were sufficient primary/secondary amine groups, the mineralization occurred on CDs rather than alginates due to the hydrogen bond between primary/secondary amine and carboxyl of alginates. Thereby, this promoted the gel-sol transition through Ca 2+ capture from the hydrogels. Moreover, antibacterial test revealed Ca 2+ capture from cell walls, while in vivo test revealed hypoxia relief due to porous structures of the renewed hydrogels. Overall, CDs with sufficient primary/secondary amine groups could dissolve Ca-alginate hydrogel through site-specific mineralization method, accompanying by additional functions of antibacterial and hypoxia relief. Graphical Abstract
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BIOCHEMICAL SYSTEMATICS AND ECOLOGY
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2025
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IF 2
Lue Hong,Zerong Wu,Jiahui Gong,Jiahui Ma,Shengzhi Liu
Alocasia cucullata (Lour.) Schott is a member of the Araceae family and is characterized by its unique leaf morphology and vibrant flowers; thus, it is a valuable addition to gardens and interior decor. However, A. cucullata may contain toxic compounds, including calcium oxalate crystals, lectins, and alkaloids, and their distribution within the plant tissues remains poorly understood. In this study, untargeted metabolomics was used to analyse the distribution of the bioactive compounds across the roots, stems, leaves, and guttation of A. cucullata . The toxic effects of these tissues on the MDA-MB-231 human breast cancer cell line were evaluated using the MTT assay. Microscopic techniques were utilized to examine the microstructure of the guttation and stems, and the focus was on the morphology of the calcium oxalate crystals. Liquid chromatography and ELISA kits were used to quantify the calcium oxalate and lectin contents in the various tissues. The results demonstrated that the active constituents, primarily fatty acids, phenylpropanoids, flavonoids, peptides, and alkaloids, were concentrated in the roots. Notably, guttation exhibited no toxicity and lacked both calcium oxalate crystals and significant lectin levels. This research enhances the understanding of the bioactive composition and safety profile of A. cucullata and provides a theoretical foundation for future scientific investigations and safe cultivation, with implications for both scientific and industrial applications.
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