INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Yumei Hu,Yuhang Xu,Biyu He,Du Niu,Xuechun Yang,Jiaping Huang,Zhenzhen Xie,Panpan Shen,Xian Li,Maosen Bai,Ziwei Liu,Hao Li,Yunyun Yang,Jian-Wen Huang,Chun-Chi Chen,Rey-Ting Guo,Longhai Dai
Ochratoxin A (OTA) is a pervasive and significant mycotoxin that poses serious health risks to humans and animals. The development of efficient biocatalysts for the enzymatic detoxification of OTA is of great importance. In this study, we enhanced the OTA-degrading activity of three amidohydrolases (ADHs) by up to ninefold. This improvement was achieved by reducing steric hindrance in the binding pocket and fine-tuning the hydrophilic interactions between the enzyme and substrate. The most efficient variant, Pw ADH/DM, was immobilized onto magnetic Fe 3 O 4 nanoparticles functionalized by the co -deposition of dopamine and polyethyleneimine. Under optimal conditions, this immobilization process achieved a high immobilization efficiency (85.4%) and activity recovery (76.9%). The immobilized enzyme exhibited enhanced pH stability and thermostability, along with good storage stability, reusability, and recyclability. More importantly, the immobilized enzyme completely degraded 100 ng/mL OTA in contaminated milk without affecting the milk's properties. These findings expand our understanding of the molecular mechanisms governing substrate binding and catalysis in OTA-degrading ADHs. Furthermore, they provide a blueprint for enzyme-based OTA decontamination during food processing.
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MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Huihui Zhang,Qi Kang,Xuwei Chen
Anthrax is a serious zoonotic disease caused by Bacillus anthracis and dipicolinic acid (DPA) is the key component and major biomarker of Bacillus anthracis, therefore the development of rapid and sensitive DPA detection method is crucial to ensure public safety. In present study, carboxyl-functionalized covalent organic framework TpPa-COOH was synthesized using 2,5-diaminobenzoic acid and 2,4,6-triformylphloroglucinol as the building blocks, then Eu 3+ was immobilized onto TpPa-COOH via coordination interaction to give the fluorescent composite TpPa-COOH@Eu 3+ of dual-emissions. The presence of DPA significantly enhanced the characteristic emission of TpPa-COOH@Eu 3+ at 616 nm attributed by the “antenna effect” between DPA and Eu 3+ , thus a ratiometric fluorescent sensor was developed for the sensitive DPA detection with a detection limit of 9.5 nmol L −1 . The ratiometric fluorescent sensor offered DPA detection with high accuracy and favorable selectivity. The practicability of fluorescence sensor was validated by detecting DPA contents in real water samples. This research not only proposes a sensitive fluorescence method for anthrax biomarker detection, aiding in the prevention of biological threats and disease spread, but also highlights the potential of COFs in developing efficient ratiometric fluorescent probes with self-calibration capabilities.
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NEW JOURNAL OF CHEMISTRY
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2026
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IF 2.6
Wensheng Wei,Chunyang Zeng,Shengli Wang,Yuxin Wang,Tiejun Yu,Xin Ma,Guangwen Xu,Jinggang Zhao,Lei Shi
An imidazole-ligated Sn catalyst ([EIMBr-COO]2Sn) with five active centers (i.e., Lewis acidic Sn center, hydrogen atoms of the methylene and methyl groups, the imidazolium cation, and a nucleophilic site Br−) for efficient cycloaddition of epoxides was designed and constructed by combining simulation and cognition. Under mild conditions for only 30 min, the propylene carbonate (PC) yield reached 99.73% with a significant TOF value of 582.37 h−1. [EIMBr-COO]2Sn also demonstrated a wide range of catalytic universality and satisfactory stability. The calculated activation energy (Ea) was much lower at 26.23 kJ mol−1 (6.27 kcal mol−1) compared to those of other reported catalysts. The DFT calculations evidently revealed the efficient catalytic reaction pathway and clearly indicated the promotion mechanism of three electrophilic sites in CO2 cycloaddition. It is elucidated that different electrophilic active sites (i.e., Lewis acid Sn, methylene protons, and methyl protons) interacted with the oxygen atom of epoxides during different reaction states and thus weakened the C–O bond, stabilized the reaction intermediate, and significantly reduced the energy barrier for the CO2 cycloaddition reaction. Informed by the dynamic demands for CO2 cycloaddition, this study employed a sequential strategy of theoretical model construction followed by experimental validation to develop an integrated, high-efficiency catalyst with combined active sites, which established a new paradigm for designing functional catalysts.
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PROGRESS IN ORGANIC COATINGS
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2026
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IF 7.9
Wenbo Sui,Yijing Chen,Yingnan Wang,Han Feng,Chunhong Zhang,Lei Su,Lei Shang
Commercial epoxy resins (EP) face key challenges such as flammability, brittleness, poor processability, and incompatibility, limiting their widespread use. This study presents the synthesis of phosphorus-based ionic liquid curing agents using n-Octyl phosphonic acid (OPA) and various imidazole derivatives through a supramolecular assembly strategy. The design of these curing agents aims to improve the flammability, brittleness, and processing performance of EP. The results show that ZMI, 1-ZMI, and B-ZMI, due to the inherent properties of ionic liquids, exhibit excellent compatibility with EP. Compared to EP/IM, EP/1-EIM, and EP/BIM, the mechanical and flame-retardant properties of EP/ZMI, EP/1-ZMI, and EP/B-ZMI are significantly enhanced, with all samples achieving UL-94 V-0 ratings. Additionally, the ionic bonds exhibit a “molecular switch” effect, extending the processing time of EP/ZMI, EP/1-ZMI, and EP/B-ZMI. Notably, EP/1-ZMI, where only the nitrogen at the 3-position of imidazole participates in the curing reaction, remained unchanged for 360 min at 60 °C, but rapidly gelled in just 4.5 min at 150 °C. This effect was not observed with other imidazole derivative curing agents with different substituents. In conclusion, all three curing agents effectively enhanced the mechanical and flame-retardant properties of EP, with 1-ZMI demonstrating superior processability. This approach offers a simple yet effective strategy for enhancing flame retardancy and toughness, with strong potential for industrial applications.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
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IF 5.4
Qiao Ding,Yuhang Liu,Ning Li,Bo Wu,Qing Cheng,Qi Zhao,Tong Liu,Rui Wang,Deyu Li
Dye-containing acid sulfate copper plating baths frequently suffer from severe dull deposits in the low-current-density (LCD) region due to the overpowering synergistic inhibition of polyether suppressors and dyes. To address this challenge, we developed a novel nicotinic acid-modified imidazole–epichlorohydrin complex (IEN1.5) designed to uniquely preserve the activity of the bis(3-sulfopropyl) disulfide (SPS) accelerator. Through a combination of electrochemical analyses, macroscopic plating tests, and DFT/MD simulations, we demonstrate that IEN1.5 physically interacts with SPS via specific non-covalent bonds rather than acting as a standalone, competing brightener. This original molecular strategy successfully breaks the severe LCD suppression, significantly enhancing deposit brightness and strengthening the Cu(111) crystallographic texture. Crucially, by preventing the detrimental over-coordination to Cu⁺ intermediates, IEN1.5 achieves unprecedented bath stability, reducing apparent SPS consumption to a mere 0.9% over 100 cyclic voltammetric stripping (CVS) cycles. These findings provide a highly original and robust mechanistic framework for designing stable, high-efficiency additive systems in advanced copper electrodeposition.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2026
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IF 9.6
Sihan Wang,Mengyao Han,Xiaoqing Wu,Fanyong Yan,Tong Lin,Xin Wei
Tough ionogels are developed via doubly ionic H-bond networks between polyacrylic (PAA) and protic ionic liquid [DBUH][Triz]. • The ionogel achieves superior fracture strength (24.3 MPa) and toughness (94 MJ·m −3 ). • Excellent dry resistance, temperature tolerance, and solvent-dependent swelling behavior. • High sensitivity and durability enable the ionogel to precisely detect diverse human motions. • Molecular insights are provided for designing mechanically tough and environmentally stable ionogels. The inherent trade-off between structural flexibility and mechanical robustness, along with difficulties with long-term environmental compatibility, severely limits the practical use of ionogels in flexible electronics. To address these limitations, we present the design of doubly ionic hydrogen bond networks and the fabrication of highly tough ionogels. These strong physical interactions are engineered by integrating poly(acrylic acid) (PAA) with a protic ionic liquid (PIL) synthesized from 1,8-diazabicyclo[5.4.0]undec-7-ene and 1,2,4-triazole. Governed by hydrogen bonds and ionic interactions, the optimized ionogel achieves remarkable mechanical performance, exhibiting a maximum tensile strength of 24.30 MPa, a fracture strain of 480%, and an exceptional toughness of 93.95 MJ·m −3 . Moreover, the ionogel demonstrates excellent anti-drying and solvent-dependent swelling behavior, as well as thermal tolerance and strong adhesion. As a flexible strain sensor, the ionogel exhibits high sensitivity and excellent electrical signal stability, enabling the reliable capture of human motion signals. The work establishes a promising strategy for fabricating mechanically robust ionogels via creating doubly ionic hydrogen bond networks. It also provides new insights for advanced flexible sensors adaptable to dry and harsh working conditions. Download: Download high-res image (176KB) Download: Download full-size image
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ChemSusChem
·
2026
·Vol. 19
·期 15
·e70925
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IF 5.9
Juanhua Kong,Xiong Zhang,Huizhen Hu,Luyao Lian,Sijie Liu,Xuehui Li
In order to tackle the challenges of biological refining, including strong acids/alkalis and high temperatures, the aerobic oxidation was implemented in the pretreatment of biomass for one‐pot extraction and separation of lignin and carbohydrates by combining the processes of biomass hydrolytic fractionation and in situ lignin oxidative modification. As expected, the yields of oxidized lignin and carbohydrate residue under optimized pretreatment condition are up to 17.1% and 59.9%, respectively, and the oxidized lignin exhibits improved depolymerization performance, giving the phenolic monomer yield of around 245.0 mg g −1 , which are higher than those of Kraft lignin (26.3 mg g −1 ) and sodium lignosulfonate (44.0 mg g −1 ) under identical conditions. Intensive investigation showed that this enhanced depolymerization performance is contributed to the existence of large number of aryl α‐ketone functional groups in oxidized lignin obtained from the oxidation of β–O–4, β–5, and β–β linkages, reducing the activation energy of C–O interlinkages. Besides, the carbon material derived from carbohydrate calcination exhibits graphene‐like structure and hierarchical porous architecture with micro‐, meso‐, and macro‐pores. It is thus believed that the lignin‐first process can significantly demonstrate unprecedented potential to accomplish the utilization of whole biomass components. Lignin‐first technology was developed by hydrolytic fractionation and in situ lignin oxidation, which resulted in 17.1% of oxidized lignin and 59.9% of carbohydrate residuce, and 24.5% yield of phenolic monomers could be obtained from the depolymerization of oxidized lignin.
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Advanced Materials Interfaces
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2026
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IF 4.5
Chunmin Jia,Yimin Lei,Hong Xu,Xin Meng,Yuxiang Wei,Peixian Li,Xiaohua Ma,Yue Hao,Xiaohui Jia
Programmed death-1 (PD-1) is a key biomarker for monitoring therapeutic response and resistance in non-small cell lung cancer (NSCLC) immunotherapy. Achieving highly sensitive, real-time detection of PD-1 is crucial for early prediction of treatment resistance and disease recurrence. However, current methods such as ELISA suffer from limitations in sensitivity, speed, and cost. To address these challenges, we develop a label-free biosensor based on semiconducting single-walled carbon nanotube field-effect transistors (CNT-FETs). The sensing interface is designed by wrapping s-SWCNTs with poly[9-(1-octylnonyl)-9H-carbazole] (PCz), enabling immobilization of anti-PD-1 antibodies via cation-π interactions. XPS analysis confirms antibody immobilization via reversible binding energy shifts in C 1s and N 1s spectra upon salt treatment, consistent with cation-π interactions, further validated by NaCl and imidazole competition assays. Using this strategy, the sensor achieves a detection limit of 8.39 fg/mL for murine PD-1—three orders of magnitude lower than ELISA, along with good specificity and favorable regeneration capability. In human serum, it shows promising performance, with 12 out of 14 NSCLC patient samples (85.7%) exhibiting good linear correlation. With rapid response, low cost, and simple operation, this platform offers a reliable strategy for point-of-care PD-1 monitoring during NSCLC immunotherapy.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9.1
Jinpu Yang,Yujiao Bi,Xuejie Meng,Fan Zhang,Zhijie Shang,Guihua Niu,Hongbing Song
Emulsified oily wastewater is difficult to separate because of the small droplet size and high interfacial stability of emulsified droplets. Herein, a series of porous poly(ionic liquid)s (PILs) with tunable wettability and pore structures were synthesized through Friedel-Crafts crosslinking reactions using cyanuric chloride and imidazole-derived ionic liquid monomers. By regulating the co-crosslinkers and crosslinkers, the surface wettability and porous architectures of the PILs were effectively tailored. The obtained materials exhibited either hydrophobic and oleophilic properties or hydrophilic and underwater oleophobic properties, enabling the selective separation of stratified oil-water mixtures and emulsified oil-water systems. For emulsified oil-water systems, the separation efficiencies exceeded 97%, with a maximum flux of 940 L·m −2 ·h −1 , while separation efficiencies above 99% and a maximum flux of 9251 L·m −2 ·h −1 were achieved for stratified oil-water mixtures. The results indicate that the co-crosslinkers primarily regulate surface wettability, whereas the rigid crosslinker promotes the formation of larger pore volumes and more efficient transport pathways, thereby enhancing permeation performance. This work establishes a structure-property relationship between co-crosslinker chemistry, pore architecture, and separation performance, providing a new strategy for the rational design of porous PIL-based oil-water separation materials.
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APPLIED CATALYSIS A-GENERAL
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2026
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IF 4.8
Huimin Luo,Jinming Yan,Deyun Xu,Jia Guo,Mengjie Fan,Longtian Kang,Qingfu Sun
P/N-type Z-scheme heterojunction of Co-PDI/Cu-CuTCPP is prepared by a simple way. • Multifunctional photocatalytic activity of is achieved for POE, PHE and CO 2 RR. • Catalytic activities for POE and CO 2 RR increase by ~3 and ~12 times, respectively. • The overall CO 2 RR in the aqueous phase can be achieved to yield O 2 , CO and CH 4 . • It is found that Co-O-Cu linkage exists and has important role in photocatalysis. Developing a multifunctional system for photocatalytic water splitting (PWS) and carbon dioxide reduction reaction (CO 2 RR) is a promising approach to achieve the green carbon cycling. Here, the p/n-type Z-scheme heterojunction (Co-PDI/Cu-CuTCPP) between cobalt perylene diimide nanorods (Co-PDI) and copper porphyrin metal-organic framework nanosheets (Cu-CuTCPP NSs) is constructed via a cetyltrimethylammonium bromide (CTAB)-assisted physical method. Systematical characterizations prove the existence of Co–O–Cu linkage, π-π interaction, and electrostatic forces between Co-PDI and Cu-CuTCPP. The effect of Co-PDI/Cu-CuTCPP ratio on the optical, photoelectric and electrical properties, as well as multifunctional photocatalytic activity is investigated and analyzed. Under visible light irradiation (λ > 420 nm), the Co-PDI/Cu-CuTCPP composites exhibit the photocatalytic activities of water splitting to H 2 and O 2 , as well as CO 2 RR to CO and CH 4 , respectively. After optimizing photocatalytic system, the oxygen evolution rate of 18.7 mmol·g −1 ·h −1 is reached, which is a 4.4-fold compared that of pure Co-PDI. The photogenerated electron conversion efficiency during CO 2 RR is 12.9-fold higher in Co-PDI/Cu-CuTCPP than in Cu-CuTCPP. Meanwhile, the overall CO 2 RR in the aqueous phase can be achieved without H 2 production. Mechanism studies reveal the photoelectrical conversion process, and confirm the synergistic role between Co-PDI and Cu-CuTCPP. Download: Download high-res image (310KB) Download: Download full-size image
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ACS Biomaterials Science & Engineering
·
2026
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IF 6
Yang Zenglin,Li Guoqiang,Ge Yuanyuan,Liu Xin,Cao Limin,Wang Kaiqiang,Wang Xiudan,Lin Hong,Zhang Shaoen,Chen Qingzhou,Sui Jianxin
Single-domain antibodies (sdAbs) derived from naive phage display libraries offer a time-efficient alternative to animal immunization but often exhibit suboptimal affinity, particularly for small-molecule haptens, where restricted binding interfaces limit the efficacy of traditional saturation mutagenesis. For instance, the wild-type sdAb (B1)-targeting ethoxyquin (EQ) exhibits a moderate equilibrium dissociation constant ( K D ) at the submicromolar level (within the 10 −7 M range), restricting its practical sensitivity. To overcome the structural and energetic barriers inherent in hapten recognition, a “structure-guided directed modification” strategy was developed that focuses on conformational tuning rather than simple side-chain replacement. Integrating AlphaFold2 modeling and AutoDock mechanistic analysis identified key interactions (e.g., the ASP19-EQ-N8 hydrogen bond). Subsequently, a random single-amino acid insertion strategy was implemented within the CDR3 loop (residues 77−90) to fine-tune local loop geometry. Top candidates were screened via MM/GBSA binding free energy calculations and experimentally validated using biolayer interferometry. This approach yielded three high-affinity mutants—77A, 79C, and 85H—with calculated ΔΔ G values of −10.56, −4.35, and −6.69 kcal/mol, respectively. The mutants achieved enhanced affinities in the tens-of-nanomolar range (down to 35.1 nM), representing up to approximately 3 times overall improvement. Importantly, orthogonal surface plasmon resonance analysis using nonconjugated, free EQ successfully verified this affinity maturation trend, showing that mutant 79C bound free EQ with a K D of 1.30 × 10 −5 M (a 2.3 times improvement over wild-type) through simultaneously accelerated association and slowed dissociation. Mechanistically, this substantial increase in affinity is attributed not only to reinforced noncovalent networks that significantly stabilize the complex but also to favorable CDR3 geometric outward flips that alleviate steric hindrance, leading to an approximate 3.8 times acceleration in the k on . Crucially, these mutants demonstrated notable anti-interference tolerance in complex aquatic food matrices (e.g., sea bass extracts). Ultimately, this work provides a practical and efficient computational framework for assisting the rapid evolution of low-affinity hapten sdAbs into high-performing biorecognition elements with potential for next-generation biosensing architectures.
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EMBO Molecular Medicine
·
2026
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IF 7.9
Liu Lijie,Yan Mengrong,Liang Ruoxu,Wu Qingxin,Qu Changbin,Wu Huiqi,Liao Zhi,Chen Huiyu,Zou Jingjing,Sun Zeyun,Niu Dongyu,Wu Liyuan,Zhao Li,Dai Jialing,Shen Jialun,Zhang Long,Zhang Wei,Peng Wei
Respiratory syncytial virus (RSV) remains a major cause of severe respiratory disease, and stabilization of the prefusion (preF) conformation of the F glycoprotein is central for vaccine development. Here, we report a structure-guided engineering strategy that enhances preF stability by reducing the hydrophobic cavity within the trimeric F protein. Targeted modifications at metastability-associated sites generated RVF-88, a disulfide-free stabilized preF immunogen that preserves key neutralizing epitopes, including antigenic site Ø, while exhibiting improved structural integrity and long-term storage stability. Formulated as an unadjuvanted bivalent vaccine, RVF-88 elicited potent neutralizing antibody responses and durable immune protection lasting up to 5 months in mice. Vaccination also protected both mice and cotton rats against RSV challenge. Structural analyses confirmed the intended cavity-reduction design, revealing a reduced apical hydrophobic cavity volume and surface area while maintaining the prefusion architecture. Together, these findings establish hydrophobic cavity reduction as a rational strategy for stabilizing prefusion RSV F and provide a promising next-generation vaccine candidate with improved stability and immunogenicity for further clinical development.
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危险品化学品经营许可证（带存储）