INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Lixi Cai,Yanqi Gao,Yunmeng Chu,Yuanqing Lin,Lixing Liu,Guangya Zhang
Tailoring magnetic nanocarriers with tunable properties is of great significance for the development of multifunctional candidate materials in numerous fields. Herein, we report a one-pot biomimetic silicification-based method for the synthesis of silica-coated magnetic nanoparticles. The synthesis process was mild, low cost, and highly efficient, which took only about 21 min compared with 4.5–120 h in other literature. Then, the carriers had been characterized by VSM, SEM, TEM , XRD, FT-IR, and EDS to confirm their function. To evaluate the usefulness of the carriers, they were adopted to couple the purification and immobilization of β-1,3-xylanase from the cell lysate in a single step with high immobilization yield (92.8 %) and high activity recovery (82.4 %). The immobilized enzyme also retained 58.4 % of the initial activity after 10 cycles and displayed good storage properties, and improved thermal stability , which would be promising in algae biomass bioconversion as well as other diverse applications.
研究领域:
生物化学
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纳米技术
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酶工程
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Chaozhong Xu,Liqun Sun,Shanshan Tong,Jia Ouyang,Xiaoli Gu
This study investigates the cellulase immobilization onto zeolitic imidazolate frameworks (ZIFs) to enable efficient cellulose hydrolysis at high solids loading. Different ZIF carriers (ZIF-8, ZIF-8-NH 2 , Fe 3 O 4 @ZIF-8, and Fe 3 O 4 @ZIF-8-NH 2 ) were prepared and characterized. The resulting ZIF-immobilized cellulases were subsequently analyzed for their loading capacity and enzyme activity , as well as their biochemical properties such as temperature stability, pH stability, storage stability, enzyme leaching, and enzyme kinetics . Results revealed that the cellulase immobilized on Fe 3 O 4 @ZIF-8-NH 2 exhibited an exceptional loading capacity of 359.89 mg/g and a relative enzyme activity of 69.39%. Remarkably, at a solid loading of 15% (w/w) and an enzyme dosage of 5 FPU/g cellulose, the Fe 3 O 4 @ZIF-8-NH 2 immobilized cellulase demonstrated a 20.44% higher hydrolysis yield compared to the free cellulase. Furthermore, this immobilized enzyme system displayed robust reusability, retaining 71.03% of its initial activity even after 10 cycles of reuse. A proposed mechanism of enhanced enzymatic hydrolysis by ZIF-immobilized cellulases was also presented. These findings have significant implications for the future application of ZIF-immobilized cellulases in efficient and cost-effective lignocellulosic bioconversion .
研究领域:
生物化学
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酶工程
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生物质转化
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Shuo Liu,Hongli Chao,Meishan Guo,Ruifang Wang,Dongyu Gu,Yi Wang,Dajun He,Yi Yang
To achieve the biotransformation of cellulose into gluconic acid, a multi-enzyme reactor comprising cellulase, glucose oxidase, and catalase was constructed through artificial antibody-antigen-directed immobilization. Catechol was used as a template to prepare artificial antibodies, which were then used to modify the enzymes through a Schiff base reaction to form artificial antigens. The artificial antibody and antigen were subsequently assembled via affinity recognition to immobilize the enzymes. The immobilization yields for all three enzymes exceeded 85 %, with specific activities of 43.48 ± 1.54 U/mg, 0.88 ± 0.06 U/mg, and 18.47 ± 1.43 U/mg, respectively. The immobilized enzymes demonstrated excellent adaptability, storage stability, reusability, and regenerability. Through the modular assembly of artificial antigen-antibody, the multi-enzyme reactor facilitated the cascade conversion of cellulose to gluconic acid.
研究领域:
生物化学
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合成生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Jianxiong Chen,Mengyao Hao,Wenjie Hou,Jingjing Zhang,Yu Xin,Rui Zhu,Zhenghua Gu,Liang Zhang,Xuan Guo
Phosphotriesterase (PTE) biodegradation of organophosphorus pesticides (OPs) is an efficient and environmentally friendly method. However, the instability and nonreusability of free PTE become the key factors restricting its practical application. In this study, a novel cross-linked magnetic hybrid nanoflower (CLMNF) was prepared. Molecular dynamics (MD) simulations were performed to further investigate the enhanced catalytic efficiency of the enzymes. The recovery rate of enzyme activity was 298% due to the large specific surface area and metal ion activation effect. More importantly, the immobilization scheme greatly improved the stability and reuse performance of the catalyst and simplified the recovery operation. CLMNFs retained 90.32% relative activity after 5 consecutive cycles and maintained 84.8% relative activity after 30 days at 25 °C. It has a good practical application prospect in the degradation and detection of OPs. Consequently, the immobilized enzyme as a biocatalyst has the characteristics of high efficiency, stability, safety, and easy separation, establishing the key step in a biodetoxification system to control organophosphorus contamination in food and the environment.
研究领域:
环境科学
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生物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2024
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IF 4.8
Hua-Wei Zhang,Ke Cheng,Jing-Jing Tang,Ling-Zhi Liu,Ruo-Xuan Qin,Yi-Nuo Sun,Jiang-Hua Zhang,Da-Yong Zhou
To synthesize structured phospholipids (SPLs) in an efficient and green manner is of great significance. Herein, novel Fe 3 O 4 @SiO 2 - Aspergillus oryzae lipase (AOL)/copper phosphate trihydrate nanoflowers (FLCNs) were prepared by coprecipitation method and then characterized by energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer etc. FLCN activity was further evaluated by promoting functional medium-chain SPL (MCSPL) or short-chain SPL (SCSPL) synthesis via biotransesterification between soybean lecithins and medium-chain fatty acids (MCFAs) or short-chain fatty acids (SCFAs) under optimal reaction conditions. Moreover, FLCNs and free AOLs were comparatively assayed in enzymatic properties. As a result, FLCNs were successfully fabricated with sufficiently large surfaces and numerous enzyme active sites. Owing to these structural features, after reacting at 40 °C for 36 h with FLCN dosage of 6% and substrate mass ratio of 1:3:3, FLCNs exhibited much more excellent activity in MCSPL synthesis with 88.30 ± 0.63% of MCFA incorporation than benchmark enzymes and heterogeneous functional materials. Most significantly, in the first biosynthesis of functional SCSPLs facilitated by FLCNs, much superior SCFA incorporation of 66.55 ± 0.73% was obtained. Notably, FLCNs also possessed convenient magnetic separability together with superior pH and temperature tolerances, thermal and storage stabilities, and recyclability to free AOLs. In brief, the present investigation provides a new biological strategy for effective, economic, convenient, and eco-friendly SPL synthesis in food and pharmaceutical industries, highlighting its merits in functional lipid production.
研究领域:
食品科学
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生物工程
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材料科学
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产品关联
DOI
高影响因子
MOLECULES
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2017
·Vol. 22
·期 1
·179
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IF 2.861
Chunfang Li,Shuhui Jiang,Xinying Zhao,Hao Liang
In this paper we report a facile method for preparing co-immobilized enzyme and magnetic nanoparticles (MNPs) using metal coordinated hydrogel nanofibers.Candida rugosalipase (CRL) was selected as guest protein. For good aqueous dispersity, low price and other unique properties, citric acid-modified magnetic iron oxide nanoparticles (CA-Fe3O4NPs) have been widely used for immobilizing enzymes. As a result, the relative activity of CA-Fe3O4@Zn/AMP nanofiber-immobilized CRL increased by 8-fold at pH 10.0 and nearly 1-fold in a 50 °C water bath after 30 min, compared to free CRL. Moreover, the immobilized CRL had excellent long-term storage stability (nearly 80% releative activity after storage for 13 days). This work indicated that metal-nucleotide nanofibers could efficiently co-immobilize enzymes and MNPs simultaneously, and improve the stability of biocatalysts.Keywords:metal;nucleotide;magnetic iron oxide nanoparticles;Candida rugoselipase (CRL);immobilized enzyme
研究领域:
生物化学
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纳米技术
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酶工程
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2011
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IF 2.692
Pixiang Wang,Xiangyu Gong,Erzheng Su,Jingli Xie,Dongzhi Wei
A facile pretreatment method was developed to obtain high specific activity of PGA from recombinant E. coli cells for preparing immobilized enzyme with high activity, which coupled selective extraction of PGA with butyl acetate and adsorption of butyl acetate with active carbon. Butyl acetate (5%, v/v) led to a 92.0% release of PGA, the specific activity of which in the extracting solution was twice that obtained by sonication. The negative effect on PGA immobilization due to residual butyl acetate in the extracting solution was removed by adding active carbon (8%, w/v). The final PGA solution meets requirement for industrial immobilization while eliminating the high cost of a traditional purification process. This pretreatment method developed in this work is simple, highly performing and cost-effective. It has been employed in a 10 tons/year immobilized PGA production line.
研究领域:
生物化学
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酶工程
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产品关联
DOI
高影响因子
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
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2019
·Vol. 24
·期 2
·326-336
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IF 1.438
Zhao Jiong-feng,Tao-Wang,Lin Jian-ping,Yang Li-rong,Wu Mian-Bin
Early research on the nutritional value of 1,3-diacylglycerols (1,3-DAGs) has resulted in a significant interest in their synthesis. 1,3-DAGs can be produced chemically and biologically. In this work, a regioselective lipase from Rhizopus oryzae was efficiently immobilized on nanosized magnetite particles (NSM) in an oriented way, resulting in significant enhancement of activity. The specific hydrolytic and esterification activities of the immobilized enzyme were 1,660% and 260% of those of the free enzyme, respectively. The immobilized enzyme was then used to catalyze the esterification of oleic acid with glycerol in a solvent-free system for preparation of 1,3-DAG in a 1 L reactor. The catalytic process was studied in detail, the final concentration of 1,3-DAG reached >76% under the optimal condition when the molar ratio of oleic acid to glycerol was 2.8:1. The regioselectivities of free and immobilized enzyme were both >97%. The immobilized enzyme was reused for 55 cycles with only ∼30% activity loss at 30°C. The purity of 1,3-DAG was up to ∼95% (w/w) after a simple purification step with the recovery ratio ∼85%. This is the first report of efficient 1,3-DAG purification by neutralization without acyl migration.
研究领域:
生物化学
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食品科学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2023
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IF 6.2
Xingguo Liu,Xue Zhang,Zhendong Fu,Xianhui Cheng,Liping Wang,Lianghai Hu
Immobilized enzymes outperform free enzymes in many properties and are widely used in environmental monitoring, engineering applications, food and medical fields. Based on the developed immobilization techniques, the search for immobilization with wider applicability, lower cost and more stable enzyme properties is of significant importance. In this study, we reported a molecular imprinting strategy for immobilizing peptide mimics of DhHP-6 on mesoporous materials . The DhHP-6 molecularly imprinted polymer (MIP) showed much higher adsorption capacity than raw mesoporous silica toward DhHP-6. The DhHP-6 peptide mimics was immobilized on the surface of mesoporous silica for the fast detection of phenolic compounds , a widely spread pollutant with highly toxic and difficult in degradation. Immobilized enzyme of DhHP-6-MIP exhibited higher peroxidase activity, better stability, and recyclability than free peptide. Notably, DhHP-6-MIP showed excellent linearity for the detection of the two phenols with detection limits of 0.28 μM and 0.25 μM, respectively. In combination with the spectral analysis and PCA method, DhHP-6-MIP provided better discrimination between the six phenolic compounds (phenol, catechol , resorcinol , hydroquinone , 2-chlorophenol, 2, 4-dichlorophenol). Our study showed that immobilization of peptide mimics by the molecular imprinting strategy using mesoporous silica as carriers was a simple and effective approach. The DhHP-6-MIP has great potentiality for the monitoring and degradation of environmental pollutants .
研究领域:
环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2016
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IF 1.685
Jinxin Che,Junling Shi,Zhenhong Gao,Yan Zhang
An enzymatic reaction system was developed and optimized for bioconversion of resveratrol from glucose. Liquid enzyme extracts were prepared from Alternaria sp. MG1, an endophytic fungus from grape, and used directly or after immobilization with sodium alginate. When the enzyme solution was used, efficient production of resveratrol was found within 120 min in a manner that was pH-, reaction time-, enzyme amount-, substrate type-, and substrate concentration-dependent. After the optimization experiments using the response surface methodology, the highest value of resveratrol production (224.40 µg/l) was found under the conditions of pH 6.84, 0.35 g/l glucose, 0.02 mg/l coenzyme A, and 0.02 mg/l ATP. Immobilized enzyme extracts could keep high production of resveratrol during recycling use for two to five times. The developed system indicated a potential approach to resveratrol biosynthesis independent of plants and fungal cell growth, and provided a possible way to produce resveratrol within 2 h, the shortest period needed for biosynthesis of resveratrol so far.
研究领域:
微生物学
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酶工程
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天然产物化学
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生物转化
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代谢工程
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 10
·2381
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IF 4.2
Liran Ji,Wei Zhang,Yifei Zhang,Binbin Nian,Yi Hu
The development of immobilized enzymes with high activity and stability is critical. Metal–organic frameworks (MOFs) have attracted much academic and industrial interest in the field of enzyme immobilization due to their unique properties. In this study, the amino-functionalized ionic liquid (NIL)-modified metal–organic framework (UiO-66-NH2) was prepared to immobilizeCandida rugosalipase (CRL), using dialdehyde starch (DAS) as the cross-linker. The results of the Fourier transform infrared (FT-IR) spectra, X-ray powder diffraction (XRD), and scanning electronic microscopy (SEM) confirmed that the NIL was successfully grafted to UiO-66-NH2. The CRL immobilized on NIL-modified UiO-66-NH2(UiO-66-NH2-NIL-DAS@CRL) exhibited satisfactory activity recovery (79.33%), stability, reusability, and excellent organic solvent tolerance. The research results indicated that ionic liquid-modified UiO-66-NH2had practical potential for application in enzyme immobilization.
研究领域:
生物化学
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酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Hongbo Suo,Lili Xu,Chao Xu,Hongyue Chen,Dinghua Yu,Zhen Gao,He Huang,Yi Hu
Magnetic chitosan nanocomposites were designed and fabricated by combining the magnetic nanoparticles Fe 3 O 4 and chitosan covalently modified by imidazole-based ionic liquids with various functional groups, to be utilized as a support matrix for immobilization of porcine pancreatic lipase (PPL). Ionic liquids modified chitosan was characterized with nuclear magnetic resonance (NMR) and the nanocomposites were characterized with Fourier transform infrared spectroscopy (FTIR), vibrating-sample magnetometer (VSM), thermogravimetry analysis (TGA), Transmission electron microscope (TEM) and Scanning electron microscopy (SEM). The enzymatic properties of PPL were significantly improved by immobilization onto all thus prepared nanocomposites, and among the supports, modified with ionic liquids bearing hydroxyl group exhibited relatively enhanced performance. Particularly, the MCS-IL(8C)-OH-PPL exhibited the highest specific activity which was 6.68 times that of free PPL, and the residual activity of MCS-IL(8C)-OH-PPL remarkably maintained 91.5% of its initial activity even after 10 repeated cycles. The residual activity of MCS-IL(8C)-OH-PPL retained 55.8% after incubated in 6 M urea solution for 1 h and maintained the noticeable initial activity of 75.5% after incubated in phosphate buffer at 50 °C with pH 7.5 for 6 h. In conclusion, the study demonstrated that Fe 3 O 4 @Chitosan nanocomposites modified with functional ionic liquids could be utilized as a novel nanosupport for enzyme immobilization .
研究领域:
生物化学
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纳米技术
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）