LWT-FOOD SCIENCE AND TECHNOLOGY
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2017
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IF 2.329
Yingnan Liu,Shaoxuan Yu,Lizhi Liu,Xiaoyue Yue,Wentao Zhang,Qingfeng Yang,Li Wang,Yanru Wang,Daohong Zhang,Jianlong Wang
In this work, the double-edged effect of curcumin (Cur) on Maillard reaction (MR) in model milk pasteurization process was demonstrated for the first time, where Cur inhibits MR at low concentrations (<5 μg/mL), while promotes it at high concentrations (>5 μg/mL). A nanocapsule strategy was introduced to resolve the disadvantages of Cur on MR at high temperature. In vitro experiments confirmed that curcumin @ polyvinylpyrrolidone nanocapsules (Cur@PVP NCs) synthesized by a one-pot method can control the continuous and slow release of Cur and exert inhibitory effect on MR by competing with lactose for casein, trapping the reactive dicarbonyl compounds and scavenging ROS, which might provide a new strategy for the application of functional food ingredients in the control of MR during food heat processing , including milk pasteurization.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2022
·
IF 3.268
Kemeng Qin,Mingyue Ding,Chensi Zhang,Xijie Zhang,Yexuan Mao,Meng Dang,Zizhe Li,Youyi Wang,Shaohui Zhang,Yuhang Sun,Zhibin Zhang,Gaiming Zhao,Yu Li,Qian Li,Xiya Zhang
To improve the detection sensitivity of neomycin (NEO) in milk, we produced a sensitive monoclonal antibody (mAb) against NEO and developed a lateral flow immunoassay based on amorphous carbon nanoparticles (ACNPs-LFA). First, we conjugated NEO to carrier protein to prepare mAbs. We obtained six mAbs: mAb 1C6, mAb 1D3, mAb 2D3, mAb 4D5, mAb 5D1, and mAb 5H1. We characterised the mAbs by indirect competitive enzyme-linked immunosorbent assay and selected the most sensitive mAb based on the half-maximal inhibitory concentration (IC50). We selected mAb 4D5 (IC50 = 0.15 ng/mL) for the development of LFA. MAb 4D5 was labelled with ACNPs (ACNPs-mAb 4D5) by electrostatic absorption. Under optimised conditions, 5.4 μg mAb 4D5 coupling with 1 mL ACNPs, NEO-OVA at concentration of 80 μg/mL, 3 μL ACNPs-mAb 4D5 were used to develop LFA. The cut-off value was 8 ng/mL. Therefore, our developed ACNPs-LFA is suitable for on-site detection of NEO residues.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaoyu Li,Fengchun Huang,Juan Zhang,Jiaci Chen,Xiangyi Pang,Longrui Yang,Xiaoyun Sun,Yuhang Fan,Ailiang Chen,Qingli Yang
Acetylcholinesterase inhibition–luciferase bioluminescence system enables rapid detection of organophosphate and carbamate pesticide residues. However, firefly luciferase (FLuc) suffers from poor stability and short luminescence duration, thereby introducing uncertainties in detection. To address this problem, nanoflower-immobilized FLuc (FLuc@NFs) was prepared using a coprecipitation method, which markedly enhanced stability and activity. FLuc@NFs exhibited intact nanoflower structures and allowed storage at 25 °C and at 4 °C for 2 weeks, overcoming the limitation of free enzyme storage at −20 °C. In the bioluminescence reaction, FLuc@NFs at 1 mg/mL achieved 62.14 % higher luminescence than free FLuc, with stable signals extending from 10 s to 30 min, enabling high-throughput detection. The system achieved detection limits of 5 and 10 ng/mL for chlorpyrifos and carbaryl, respectively, demonstrating improved reliability and sensitivity in pesticide detection.
研究领域:
生物化学
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Luan Huang,Xin Zhou,Qingyun Chang,Shuaihua Zhang,Zhi Wang,Chun Wang,Xiaohuan Zang
Sulfonamide antibiotics have a broad spectrum of antibacterial action and are widely used, but their overuse poses a threat to human health. In this study, a three-dimensional conjugated microporous polymer, which was designated as TPM-CMP, was synthesized via Friedel-Crafts reaction by using tetraphenylmethane (TPM) and biphenyl dichlorobenzene as monomers, and it was utilized as an adsorbent in solid-phase extraction (SPE) of sulfonamides. The TPM-CMP demonstrated high extraction efficiency for sulfonamides due to π -stacking interactions, hydrophobic forces, and pore-filling effects. Consequently, the SPE was combined with liquid chromatography to detect sulfonamides in milk samples. For this method established in this study, a good linear response was observed in the range of 3.3–300.0 ng g −1 , with a detection limit ( S/N = 3) between 1.0 and 1.5 ng g −1 . The method recoveries of four sulfonamide in milk samples ranged from 83.4 % to 117 %, with relative standard deviations below 10 %.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yifei Wang,Tengsheng Xie,Ji Yang,Meng Lei,Jing Fan,Zihui Meng,Min Xue,Lili Qiu,Fenglian Qi,Zhe Wang
As a typical antibiotic that is harmful to the health of human beings and widely used in animal husbandry, oxytetracycline (OTC) has a potential threat to the food safety. Therefore, the design of portable sensors for efficiently monitoring trace amounts of OTC in foods is vital to maintain safe food supply in our daily life. Herein, a two-dimensional (2D) molecularly imprinted photonic crystal hydrogel (MIPCH) sensor for the detection of OTC was fabricated by combining the photonic crystal (PC) method and molecular imprinting technique. In this regard, OTC was used as imprinted template to form the MIPCH sensor with specific binding sites during polymerization. After the removal of template, the obtained MIPCH possessed the specific nanocavities that were complementary to target compound, OTC. In addition, the response of the MIPCH to tetracyclines (TCs) in aqueous solution was monitored through a readable change of Debye diffraction ring, which is related to the particle spacing variation of MIPCH in response to the target molecules. As the concentration of OTC increased from 0 to 60 μM, the particle spacing of MIPCH sensor increased to about 94 nm and the structural color redshifted from blue to red. More importantly, the detection of OTC in milk sample by this portable, cost-effective MIPCH sensor has also been achieved at the same range of OTC. With the increasing concentration of OTC in milk sample, the particle spacing of MIPCH sensor increased about 92 nm and the structural color of MIPCH changed from blue through green to orange.
研究领域:
食品安全
·
传感器技术
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Zu Qiang Jiang,Jian Ping Wang,Peng Lei Cui
Background There have been some immunoassays reported for screening of the residues of estrogens in milk, but these methods can only determine one drug because of the limited recognition abilities of the used antibodies. Due to the broad specific recognition ability, receptor can be used as “special antibody” to develop pseudo immunoassay. However, there has been no article reporting the use of estrogen receptor for determination of estrogens in foods of animal origin so far. Furthermore, the recognition mechanism of estrogen receptor for estrogens have not been thoroughly studied. Results In this study, a type of magnetic probe based on 17β-estradiol was synthesized that was used to produce the natural porcine estrogen receptor with ovarian as the source tissue. Then its recognition mechanisms for 13 estrogens were studied based on the optimal homological model. Results showed it only interacted with estrogens but did not interact with other steroid hormones. Then it was utilized as a broad specific recognition reagent to develop a direct competitive method on 96-well microplate for detection of the 13 drugs in milk. Due to the utilization of streptavidin-biotin labeled horseradish peroxidase as signal amplification system, the sensitivities for the 13 estrogens (limits of detection 0.007-0.0573 ng/mL) were improved for 33-100 folds in comparison with the use of conventional horseradish peroxidase system (limits of detection 0.31-3.53 ng/mL). This method showed no cross-reactivity with other steroid hormones, consistent with the docking result. Significance This is the first study reporting the use of magnetic probe to produce natural receptor from animal tissues, and this is also the first study reporting a natural estrogen-receptor-based method for multi-determination of estrogens in food sample. With the guidance of this study, more receptors and related analytical methods for other drugs or small molecules maybe are reported in the future.
研究领域:
食品安全
·
生物化学
·
药物分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROPHORESIS
·
2024
·
IF 3
Weiyan Li,Chuansheng Sun,Haiping Wang,Qingyan Bai,Yi Xu,Chunmiao Bo,Junjie Ou
Detection of florfenicol (FF) residues in animal-derived foods, as one of the most widely used antibiotics, is critically important to food safety. The fluorescent molecularly imprinted polymer (MIP) was synthesized by surface-initiated atom transfer radical polymerization technique with poly(glycidyl methacrylate- co -ethylene glycol dimethacrylate) microspheres, 4-vinylpyridine, ethylene glycol dimethacrylate, and FF as the matrix, functional monomer, crosslinker, and template molecule, respectively. Meanwhile, N–S co-doped carbon dot (CD) was synthesized with triammonium citrate and thiourea as precursors under microwave irradiation at 400 W for 2.5 min and then integrated into FF-MIP to obtain CD@FF-MIP. For comparison, non-imprinted polymer (NIP) without FF was also prepared. The adsorption capacity of CD@FF-MIP to FF reached 53.1 mg g −1 , which was higher than that of FF-MIP (34.7 mg g −1 ), whereas the adsorption capacity of NIP was only 17.3 mg g −1 . The adsorption equilibrium of three materials was reached within 50 min. Particularly, CD@FF-MIP exhibited an excellent fluorescence quenching response to FF in the concentration range of 3–50 µmol L −1 . As a result, CD@FF-MIP was successfully utilized to extract FF in milk samples, which were analyzed by high-performance liquid chromatography. The standard recoveries were 95.8%–98.2%, and the relative standard deviation was 1.6%–4.2%. The method showed the advantages of simple operation, high sensitivity, excellent selectivity, and low cost, and also demonstrated a great application prospect in food detection.
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Bin Li,Wenjing Gao,Ling Ling,Shaoning Yu,Liangqiang Chen,Fan Yang
Lactose is an important nutrient but its content in milk products poses concerns for people with lactose intolerance . Thus, a simple, rapid, and high-throughput assay for lactose determination is needed to maintain specified lactose levels during production and in final milk products. Here, we report a reactive matrix-assisted laser desorption/ionization mass spectrometry ( Re MALDI MS) method for the determination of lactose in milk products. The selective reactivity and the excellent laser absorption properties of 4-hydrazinoquinazoline (4-HQ) matrix increase the sensitivity of traditional MALDI MS analysis of lactose by 100 folds. After optimization of the method, lactose was detected distinctly in milk without pretreatment . Using xylobiose as an internal standard, the assay accurately quantified lactose in the concentration range of 1.00−700 μg/mL (R 2 = 0.994). Due to the high sensitivity and specificity, the lactose content in different types of milk samples was determined successfully, as well as the residual lactose in low-lactose and lactose-free milk.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Plasmonics
·
2025
·
IF 4.3
Chen Kun,Ma Chaoqun,Chen Guoqing,Yang Taiqun,Gao Hui,Li Lei,Hu Anqi,Cao Jun,Zheng Chenkai,Ma Longyao,Chen Zehao
The detection of pefloxacin (PEF) in milk is of significant importance for food safety. Traditional methods for detecting PEF often rely on techniques such as high-performance liquid chromatography (HPLC), which are complex and time-consuming. This article introduces a surface-enhanced Raman scattering (SERS) substrate based on a composite of covalent organic frameworks (COFs) and silver nanoparticles (AgNPs) for the rapid detection of PEF in milk. After testing PEF in both water and milk, the detection limits were calculated to be 6.31 μg/L for water and 82 μg/L for milk, with correlation coefficients of 0.991 and 0.998, respectively. Furthermore, the average recovery rate of PEF in milk ranged from 96.25% to 106.17%, with a relative standard deviation (RSD) of 4.32% to 8.88%. This work offers a simple and rapid method for detecting PEF in milk. Graphical
研究领域:
食品安全
·
分析化学
·
药物残留检测
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiacheng Tao,Niu Feng,Yu Zhang,Yiping Chen,Zhen Zeng,Hong Jiang,Jimei Ma
The improper and excessive use of chloramphenicol has placed a heavy burden and adverse impact on the environment and human health. Herein, a fluorescent molecule with aggregation-induced emission characteristic is designed, synthesized, and encapsulated into polystyrene nanospheres, serving as an optical signaling probe in biosensors for ultrasensitive, rapid and accurate detection of chloramphenicol in milk. In this method, magnetic nanoparticles coupled with chloramphenicol antibody are used as immunomagnetic separation carriers. The polystyrene microspheres embedding aggregation-induced emission molecules are modified with complete antigen of chloramphenicol and used as stable and efficient signal probes. Chloramphenicol in sample and complete antigen modified-polystyrene microspheres are competitively bound to the immuno-magnetic carrier. The concentration is determined by the fluorescence intensity of the binding magnetic complex. This optical immunosensor presents an ultra-wide linear range (100 pg/mL–500 ng/mL), extraordinary sensitivity (limit of qualification was 100 pg/mL, 10-fold sensitive than enzyme-linked immunosorbent assay), and rapid detection speed (∼40 min). Accuracy is strong concordant with that of HPLC and ELISA in the test of milk samples. This strategy exhibits notable sensitivity, stability, specificity and reproducibility. The detection time is considerably shortened and the procedure is streamlined. It is a potential method for monitoring antibiotic contamination in a variety of settings, including the environment, food, and clinical circumstances.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Huanhuan Li,Wei Sheng,Md. Mehedi Hassan,Wenhui Geng,Quansheng Chen
The abuse of antibiotics has caused gradually increases drug-resistant bacterial strains that pose health risks. Herein, a sensitive SERS sensor coupled multivariate calibration was proposed for quantification of antibiotics in milk. Initially, octahedral gold-silver nanocages (Au@Ag MCs) were synthesized by Cu 2 O template etching method as SERS substrates, which enhanced the plasmonic effect through sharp edges and hollow nanostructures. Afterwards, five chemometric algorithms, like partial least square (PLS), uninformative variable elimination-PLS (UVE-PLS), competitive adaptive reweighted sampling-PLS (CARS-PLS), random frog-PLS (RF-PLS), and convolutional neural network (CNN) were applied for TTC and CAP . RF-PLS performed optimally for TTC and CAP (Rc = 0.9686, Rp = 0.9648, RPD = 3.79 for TTC and Rc = 0.9893, Rp = 0.9878, RPD = 5.88 for CAP). Furthermore, the detection limit of 0.0001 µg/mL for both TTC and CAP was obtained. Finally, satisfactory ( p > 0.05) results were obtained with the standard HPLC method. Therefore, SERS combined RF-PLS could be applied for fast, nondestructive sensing of TTC and CAP in milk.
研究领域:
分析化学
·
食品科学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2025
·
IF 2.2
Xinyue Yin,Qi Pu,Chumeng Wang,Yuhong Xiang,Nengsheng Ye
Kanamycin (KANA) plays a key role in the treatment of bacterial infections and has been widely used in animal husbandry. However, its overuse causes antibiotic residues in animal-derived foods. Determination methods for KANA are urgently needed for food safety. Most of the developed fluorescent aptamer sensors for detecting KANA use parental aptamer (kana-Apt) as recognition unit. However, excessive bases tend to form secondary structures and lead to high background or nonspecific signals. In this study, two fluorescent sensors based on one (kana1-Apt) and two (kana1/kana2-Apt) split fragments were developed for KANA detection. The LODs of the kana1-Apt/ThT system and kana1/kana2-Apt/ThT systems were 4.88 nM and 4.53 nM, respectively. In addition, satisfactory recoveries of the kana1-Apt/ThT system and kana1/kana2-Apt/ThT system were obtained in the detection of KANA in milk, which were 97.6%-104.5% and 98.4%-105.9%, respectively. Moreover, the results indicated that the kana1-Apt fragment plays a critical role in recognition. In conclusion, the results of the present study provide a novel strategy for molecular detection based on split aptamers.
研究领域:
食品安全
·
生物传感
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）