FOOD CHEMISTRY
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2024
·
IF 8.5
Linghui Huang,Yue Tang,Jianxun Han,Xiaojuan Niu,Xin Lin,Yuangen Wu
Malathion causes a serious threat to human health due to its widespread use in the environment. Herein, a novel and stable smartphone-integrated colorimetric biosensor for malathion detection is firstly established based on aptamer-enhanced laccase-mimicking activity. The results indicate that the M17-F aptamer can increase the affinity of Ag 2 O nanoparticles to the substrate 2,4-dichlorophenol and enhance their laccase-mimicking activity. Thus, abundant semiquinone radicals are produced in the catalytic system, which are combined with chromogenic agent to generate dark red products. The corresponding RGB values for the colour change of the solution can be easily obtained using smartphones, which is used for the rapid detection of malathion. The established biosensor for malathion has a limit of detection as low as 5.85 nmol·L −1 , and displays good selectivity for other competitive pesticides. Moreover, further studies have verified the applicability of the biosensor in actual samples, indicating that it may have the potential for application in malathion detection in food.
研究领域:
生物化学
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食品科学
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环境科学
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DOI
PubMed
高影响因子
Biotechnology Journal
·
2024
·Vol. 19
·期 6
·2400040
·
IF 3.2
Shibo Jiang,Ying Lin,Suiping Zheng
IMP (inosinic acid) is a crucial intermediate in the purine metabolic pathway and is continuously synthesized in all cells. Besides its role as a precursor for DNA and RNA, IMP also plays a critical or essential role in cell growth, energy storage, conversion, and metabolism. In our study, we utilized the circularly permuted fluorescent protein (cpFP) and IMP dehydrogenase to screen and develop the IMP biosensor, IMPCP1. By introducing a mutation in the catalytically active site of IMPCP1, from Cys to Ala, we disrupted its ability to catalyze IMP while retaining its capability to bind to IMP without affecting the IMP concentration in the sample. To immobilize IMPCP1, we employed the SpyCatcher/SpyTag system and securely attached it to Magarose-Epoxy, resulting in the development of the IMP rapid test kit, referred to as IMPTK. The biosensor integrated into IMPTK offers enhanced stability, resistance to degradation activity, and specific recognition of IMP. It is also resistant to peroxides and temperature changes. IMPTK serves as a rapid and stable assay for analyzing IMP concentrations in fermentation broth. Within the linear range of IMP concentrations, it can be utilized as a substitute for HPLC. The IMPTK biosensor provides a reliable and efficient alternative for monitoring IMP levels, offering advantages such as speed, stability, and resistance to environmental factors.
研究领域:
生物化学
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生物技术
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分子生物学
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DOI
PubMed
高影响因子
METABOLIC ENGINEERING
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2025
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IF 6.8
Shan Yuan,Chao Xu,Miaomiao Jin,Xinglin Jiang,Wei Liu,Mo Xian,Ping Jin
Aldehydes are ubiquitous metabolites in living cells. As reactive electrophiles, they have the capacity to form adducts with cellular protein thiols and amines, leading to potential toxicity. Dynamic regulation has proven to be an effective strategy for addressing the accumulation of toxic metabolites. However, there are limited reports on applying dynamic control specifically to mitigate aldehyde accumulation. In this study, the cinnamaldehyde accumulation in the biosynthesis of cinnamylamine was used as a model to evaluate a two-way dynamic regulation strategy. First, we utilized whole-genome transcript arrays to identify the cinnamaldehyde-responsive promoters: the upregulated promoter P 4 and the downregulated promoter P d . They were then employed as biosensors to dynamically regulate the synthesis and consumption of cinnamaldehyde, mitigating its toxic effects on the host. This strategy successfully reduced cinnamaldehyde accumulation by 50% and increased the production of cinnamylamine by 2.9 times. This study demonstrated a cinnamaldehyde-induced autoregulatory system that facilitated the conversion of cinnamic acid into cinnamylamine without the need for costly external inducers, presenting a promising and economically viable approach. The strategy also serves as a reference for alleviating the inhibitory effects of other toxic aldehydes on microorganisms. Additionally, the biosensors (P d and P 4 ) can respond to a range of aldehyde compounds, offering a rapid and sensitive method for detecting toxic aldehydes in both environmental samples and microorganisms, thus provide a valuable tool for screening strains enhanced aldehyde yield.
研究领域:
合成生物学
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代谢工程
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DOI
PubMed
高影响因子
NANOTECHNOLOGY
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2019
·Vol. 31
·期 9
·095501
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IF 3.399
Jun Cai,Liyun Ding,Panfeng Gong,Jun Huang
For the early diagnosis of gastric cancer, microRNA-148a (miRNA-148a) as a promising biomarker is measured by a simple colorimetric biosensor due to its unique surface plasmon resonance (SPR) absorption of gold nanoparticles (AuNPs). In the assay system, the sensing probes are facilitated by the conjugation of AuNPs with RNA probes (RNAP) via Au–S bonds, which align in a tail-to-tail fashion onto the target RNA. When miRNA-148a is introduced, a sandwich hybridization reaction is triggered between the AuNP–RNAP conjugates and targets, resulting in changes in the SPR absorption band, microscopic distribution and macroscopic color of the AuNP solution. Following this principle, this colorimetric method is able to quantitatively detect miRNA-148a at nanomolar level with a limit of ∼1.9 nM, and exhibits high sensitivity and selectivity by a low-cost UV–vis spectrometer or even the naked eye. Moreover, the AuNP network materials with a characteristic sharp 'melting transition' provide significant guidance for the reusability of DNA or RNA biosensors.
研究领域:
分子生物学
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医学诊断
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纳米技术
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DOI
PubMed
高影响因子
RSC Advances
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2015
·Vol. 5
·期 73
·59603-59608
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IF 3.84
Shuguang Yan,Yurong Tang,Mengling Yu
Herein we developed a highly sensitive methodology for heparin detection based on a resonance Rayleigh scattering (RRS) spectral assay. Heparin and concanavalin A (conA) densely stacked together and formed nanoparticles or a well-defined brick-and-mortar nanostructure. A strong electrostatic attraction, hydrogen bond and the solid–liquid interface induced RRS enhancement. The result of absorption spectra and TEM testified that the introduction of heparin indeed changed the structure of conA and formed brick-and-mortar nanostructures. The optimum reaction conditions of the method and the reasons for RRS enhancement are also discussed. Combined with an RRS spectral assay, an effective biosensor has been developed for heparin. The bioassay allows sensitive detection of heparin with a detection limit of 2.48 ng mL−1 (3σ), a linear range from 8.28 ng mL−1 to 2.5 μg mL−1. The method was successfully applied to the determination of heparin in heparin sodium injection samples.
研究领域:
分析化学
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生物医学工程
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药学
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DOI
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2018
·Vol. 124
·期 2
·1-5
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IF 1.604
Yuan Hongwen,Ma Chi,Geng Junlong,Zhang Liqiang,Cui Hai,Liu Cunzhi
Calcium ion (Ca 2+ ) is an important ion involved in body life activities, and its content detection in biomedical field owns great significance. In this study, we fabricated Co 3 O 4 conical nanotube on F-doped tin oxide (FTO) substrate for detecting Ca 2+ . Co 3 O 4 is fabricated through a hydrothermal method and demonstrates a regular hexagon structure, with a length of 5–10 μm and wall thickness of 30 nm. The structure and morphology of Co 3 O 4 were characterized by X-ray diffraction (XRD), scanning electron microscope, and transmission electron microscopy, respectively. In addition, then, we used electrochemical technique to characterize the Ca 2+ concentration in the simulated body fluid. The detection of Ca 2+ is originated from the electrochemical reaction of hydrogen peroxide using Co 3 O 4 as a catalyst, in which Ca 2+ plays a significant role for accelerating the decomposition of hydrogen peroxide catalytic performance. By monitoring the electron transfer signals changes during the electrochemical reaction, we can quickly quantify the Ca 2+ concentrations. It is found that this Ca 2+ sensor owns a wide detection range (0.1–1.1 mM), a low detection limit (3.767 μM), and good anti-interference ability.
研究领域:
材料科学
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生物医学工程
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电化学
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产品关联
DOI
高影响因子
Biosensors-Basel
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2023
·Vol. 13
·期 5
·560
·
IF 5.4
Tingting Lang,Meiyu Xiao,Wenyang Cen
Organophosphate insecticides with broad spectrum and high efficiency make a great difference to agricultural production. The correct utilization and residue of pesticides have always been important issues of concern, and residual pesticides can accumulate and pass through the environment and food cycle, resulting in safety and health hazards to humans and animals. In particular, current detection methods are often characterized by complex operations or low sensitivity. Fortunately, using monolayer graphene as the sensing interface, the designed graphene-based metamaterial biosensor working in the 0–1 THz frequency range can achieve highly sensitive detection characterized by spectral amplitude changes. Meanwhile, the proposed biosensor has the advantages of easy operation, low cost, and quick detection. Taking phosalone as an example, its molecules can move the Fermi level of graphene with π–π stacking, and the lowest concentration of detection in this experiment is 0.01 μg/mL. This metamaterial biosensor has great potential in detecting trace pesticides, and its application in food hygiene and medicine can provide better detection services.
研究领域:
食品科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2023
·
IF 9.8
Junhua Chen,Manjia Chen,Hui Tong,Fei Wu,Yizhang Liu,Chengshuai Liu
A fluorescence biosensor was developed for the ultrasensitive detection of the available lead in soil samples by coupling with DNAzyme and hairpin DNA cyclic assembly. The biorecognition between lead and 8-17 DNAzyme will cleave the substrate strands (DNA2) and release the trigger DNA (T), which can be used to initiate the DNA assembly reactions among the hairpins (H1, H2, and H3). The formed Y-shaped sensing scaffold (H1-H2-H3) contains active Mg 2+ -DNAyzmes at three directions. In the presence of Mg 2+ , the BHQ and FAM modified H4 will be cleaved by the Mg 2+ -DNAyzme to generate a high fluorescence signal for lead monitoring. The linear range of the fluorescence biosensor is from 1 pM to 100 nM and the detection limit is 0.2 pM. The biosensor also exhibited high selectivity and the nontarget competing heavy metals did not interfere with the detection results. Compare with the traditional method (DTPA+ICP-MS) for the available lead detection, the relative error (Re) is in the range from −8.3 % to 9.5 %. The results indicated that our constructed fluorescence biosensor is robust, accurate, and reliable, and can be applied directly to the detection of the available lead in soil samples without complex extraction steps.
研究领域:
环境科学
·
分子生物学
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DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Xinpei Li,Min Yuan,Min Li,Jing Li,Siyao Zhang,Fei Xu
A fluorescent biosensor was synthesized for sensitive detection of lead ions by integrating aptamer with ion imprinted polymer. ZnSe@ZnS quantum dots served as the fluorescence indicator and aptamers served as recognition elements. Specific and rapid detection of lead ions was achieved through imprinting cavities containing aptamers with specific conformations. The resulting biosensor, after optimization via the multi-factor response surface methodology, can detect lead with a detection limit as low as 0.56 nmol·L −1 (the detection limit in aquatic products is 47.73 μg/kg). Furthermore, a paper-based biosensor was developed using glass cellulose paper as the carrier material for the portable and rapid determination of lead ions in aquatic products. The proposed paper-based biosensor significantly reduces the detection time from ten minutes to one minute, and the recoveries ranged from 88.06% to 107.29%, which were validated using the ICP-MS method. These findings demonstrate that the biosensor provides a sensitive and rapid method for detecting heavy metals in complex food matrices.
研究领域:
分析化学
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环境科学
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食品安全
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纳米技术
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生物工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Jialing Zhou,Hui Huang,Shunji Chen,Mengyuan Wang,Danna Zhao,Jun Yu,Senlin Jin,Yuan Zhong,Xiaoming Chen,Xiaocai Yu,Pengbo Liu,Jian Zhao
As 3rd generation semiconductor, GaN material is promising for biosensor application, owing to its excellent chemical stability and high carrier mobility. Herein, GaN porous-layer (GNP) was self-assembly grown on GaN-coated sapphire substrate without doping by using simple low-temperature (LT) growth. The GNP with a pore density as high as ∼2.1 × 10 3 μm −2 can be realized by optimizing the growth temperature (620 °C) and layer thickness (22.4 nm), and its normalized conductivity can be tuned by simply changing the thickness. The optimized GNP was applied to chemiresistive detection of Cu 2+ with a limit of detection (LOD) as low as 8 fM, a fast response speed of 1.5 min, a wide linear range (8 fM∼8 nM) and good repeatability. The LOD was improved 1.25∼10 6 fold compared with that of previously reported biosensor. The GNP biosensor features high sensitivity, simple structure, integratability, and low-cost. To our knowledge, it is the first time that GaN material was used for chemiresistive biosensor.
研究领域:
材料科学
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生物传感技术
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纳米技术
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2011
·
IF 3.37
Cheng Fang,Jishan He,Zhencheng Chen
A disposable amperometric biosensor for detecting the total cholesterol was fabricated which comprises a sensing electrode and a reference electrode in simultaneous contact with an integrated reagent layer. The integrated reagent layer formed by coating a working ink containing cholesterol esterase, cholesterol dehydrogenase, coenzyme , redox mediator , surfactant, stabilizer, filler and at least one aqueous thickening agent . The biosensor showed the linearity for 50–500 mg/dL cholesterol acetate. The minimum detection limit of the cholesterol was 50 mg/dL. The effects of temperature and the stability of immobilized enzymes were also studied. A good correlation was found among cholesterol values obtained by commercial colorimetric test strip and clinical/laboratory methods. The biosensor showed an acceptable reproducibility, good stability and low interferences.
研究领域:
生物医学工程
·
分析化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Caihong Zhou,Jingjing Fan,Liangyuan Wang,Baoxue Gao,Chunhui Wei,Long Ma,Lijuan Yin
Norovirus (NoV) is a leading cause of nonbacterial acute gastroenteritis, primarily transmitted through contaminated food and water. Rapid, sensitive, and on-site detection is crucial for controlling NoV outbreaks and ensuring food safety. In this study, we developed a portable, one-tube CRISPR/Cas12a-driven colorimetric biosensor for the rapid and sensitive detection of NoV RNA without the need for pre-amplification. The biosensor integrates magnetic nanoprobes with gold nanoparticle (AuNP) bioprobes, connected via single-stranded DNA (ssDNA) linkers. Upon the specific recognition of NoV cDNA, the Cas12a protein’s trans -cleavage activity is activated, cleaving the ssDNA linkers and releasing AuNP bioprobes from the magnetic nanoprobes. This release results in a visually detectable red color change and enhanced UV–visible absorbance in the solution following magnetic separation. These changes can be easily detected using portable devices such as microplate readers or smartphones. This method achieves a detection limit of 10 copies/µL within 60 min, with no cross-reactivity from other viruses or bacteria. Validation using oyster samples showed perfect concordance with qPCR results. Designed for resource-limited settings, our biosensor can be adapted for detecting various pathogens by altering the target nucleic acids and crRNA sequences. This advancement offers significant potential for enhancing rapid diagnostics and outbreak management.
研究领域:
分子生物学
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食品科学
·
诊断技术
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DOI
高影响因子
危险品化学品经营许可证（带存储）