JOURNAL OF ETHNOPHARMACOLOGY
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2020
·
IF 3.69
Qingqing Chen,Rongrong Ren,Qingqing Zhang,Jingjing Wu,Yufeng Zhang,Mingsong Xue,Dengke Yin,Ye Yang
Ethnopharmacological relevance Coptis chinensis Franch is one of the most widely used traditional Chinese herbs in China and was firstly recorded in "Shennong's Classic of Materia Medica” in the Han Dynasty. The medical records in past thousands years have fully confirmed the clinical efficacies of Coptis chinensis Franch against intestinal diseases . The polysaccharides in herbal medicines can be digested by the flora and uptaken by the Peyer's patches (PPs) in intestine. It can be reasonably presumed that the polysaccharides in Coptis chinensis Franch (CCP) should be one of the critical element in the regulation of intestinal microenvironment. Aim of the study This study intended to explore the dynamic regulation of CCP on intestinal microenvironment from the perspective of the intestinal mucosal immunity and the intestinal flora , in order to provide a new research perspective for the pharmacological mechanism of Coptis chinensis Franch. Materials and methods The absorption and distribution of CCP in intestinal tissues were observed after the perfusion of FITC labeled CCP. The influences of CCP on intestinal flora were evaluated by the 16sRNA gene illumina-miseq sequencing after gavage. The regulations of CCP on intestinal mucosal immunity were evaluated by the immunohistochemical analysis of the interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) secretion in PPs and intestinal epithelial tissue. Results With the self-aggregation into particles morphology, CCP can be up-taken by PPs and promote the IFN-γ, IL-4, IL-17 and TGF-β secretion in PPs in a dose-dependent manner. The CCP can also be utilized by the intestinal flora and dynamically regulate the diversity, composition and distribution of the intestinal flora. The temporal regulations of CCP on IFN-γ, IL-4, IL-17 and TGF-β secretions in intestinal epithelial tissues are consistent with the variation tendency of intestinal flora. Conclusion CCP can provide effective, dynamical and dose-dependent regulations on intestinal microenvironment, not only the intestinal flora but also the PPs and intestinal epithelium related immune response. These may be involved in the multiple biological activities of Coptis chinensis Franch.
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SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Zongyan Quan,Hui He,Hang Zhou,Yuting Liang,Lei Wang,Songya Tian,Hongxiang Zhu,Shuangfei Wang
The low sensitivity of fluorescent sensors limits the on-site and visual detection of seafood freshness. To address these issues, a novel intelligent nanofiber ratiometric fluorescent sensor, known as a BAsCNF, was designed using cellulose nanofibers (CNFs) produced by TEMPO oxidation (TO-CNFs) as the skeleton, and fluorescein isothiocyanate and proporphyrin IX modified nanofibers as the indicator and internal reference, respectively. TO-CNFs with nano-scale characteristics improved the air permeability of BAsCNF films, which endowed the sensor with excellent response performance to biogenic amines (BAs). Thus, the BAsCNF produced obvious colour changes from red to yellow-green in response to an increased concentration of BAs, where the limit of detection for BAs was as low as 1 ppm. Meanwhile, the discoloration trend of the BAsCNF in the freshness detection of shrimp and pork was consistent with the variation of the key indicators during storage, such as the content of total volatile basic nitrogen and the total number of colonies. The results suggest that the BAsCNF sensor possessed high detection accuracy and broad application prospects in the field of food safety.
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FOOD CHEMISTRY
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2020
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IF 6.306
Hailong Sun,Shuangping Liu,Jieqi Mao,Ziwei Yu,Zhijie Lin,Jian Mao
Although huangjiu is a popular alcoholic beverage in China, the occurrence of quick-intoxication suppresses huangjiu consumption and impedes development of the huangjiu industry. In this study, the Cryprinus carpio intoxication model was used to compare the differences in intoxication effect of alcoholic beverages and to assess the impacts of huangjiu components on intoxication for the first time. Exposure to huangjiu led to the most rapid physical imbalance of C. carpio , followed by red wine and Western liquor. Higher alcohols, biogenic amines and aldehydes could cause physical imbalance of fish by themselves, and synergistic effects were observed when combined with ethanol. 2-Phenylethanoland and isopentanol had the greatest positive effect on huangjiu intoxication, followed by histamine and phenethylamine. No synergistic effect was observed between individual aldehydes and ethanol. Identification of these impactful huangjiu components provides a new perspective on the establishment of more rigorous control on the quality and flavor of huangjiu.
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JOURNAL OF SEPARATION SCIENCE
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2020
·Vol. 43
·期 13
·2728-2736
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IF 2.878
Liu Song,Yang Huang,Marie-Jia Gou,Jacques Crommen,Zhengjin Jiang,Yifan Feng
Biogenic amines have been reported in many foods such as fish, meat, and soy sauce. The consumption of foods containing high concentrations of biogenic amines has been associated with health hazards. In this study, a green and efficient method using supercritical fluid chromatography coupled with single quadrupole mass spectrometry was developed for determination of biogenic amines in soy sauce. The chromatographic and mass spectrometry conditions were systematically optimized in terms of selectivity and peak shape. Nine biogenic amines were well separated within 25 min on a Cosmosil 5HP column using 5% (v/v) water and 0.2% (v/v) ammonia solution in methanol as mobile phase additives at a backpressure of 120 bar and temperature of 40°C. The established method was fully validated regarding the linearity, sensitivity, precision, and accuracy. The limits of detection and limits of quantification ranged from 0.03 to 10.50 μg/mL and 0.10 to 23.1 μg/mL, respectively. The relative standard deviations for intra- and interday precisions were all lower than 9.36% and the recoveries ranged from 75.82 to 99.63% and 80.10 to 99.89% for two levels of standards spiked in soy sauce, respectively. Finally, the established method was successfully applied to the quantitative analysis of biogenic amines in soy sauce.
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JOURNAL OF CHROMATOGRAPHY A
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2019
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IF 3.858
Enming Miao,Nazhen Zhang,Shiwei Lu,Yufeng Hu,Lin Fu,Hao Zhou,Jingjing Zhan,Minghuo Wu
A simple magnetization method was developed for the preparation of magnetic materials from conventional solid phase packing though coprecipitation and solvothermal approaches. And the prepared magnetic materials were used for magnetic solid phase extraction (MSPE) of biogenic amines (BAs) from beers. Furthermore, to improve the analytical throughput, a solid phase “on-situ” quadraplex isotope dimethyl labeling method was developed for the quantification of BAs by liquid chromatography-high resolution mass spectrometry (LC HRMS). Compared to conventional in-solution labeling, the “on-situ” labeling could simplify the sample preparation procedure and efficiently remove the residuals such as inorganic salts and excessive labeling reagents. The quadraplex labeling, which enabled three real samples and one internal standard sample to be analyzed simultaneously in a single LC HRMS run. For the tested 8 BAs (cadaverine, phenethylamine, spermine, spermidine, tyramine, histamine, putrescine and tryptamine), LODs of 0.02–0.05 μg/L and LOQs of 0.05–0.1 μg/L were achieved at good reproducibility (RSD of 0.5–4.6% and 2.2–7.0% for intra- and inter-day reproducibility, respectively). With this method, six beer samples were analyzed, and these 8 BAs were all detected in the range of low μg/L to 2.9 mg/L, which were lower than maximal residual level (MRL) required in the regulations of China and EU.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
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IF 2.931
Yao Tian,Zhipeng Zhang,Nan Gao,Pengcheng Huang,Fang–Ying Wu
In this work, a label-free, selective, and sensitive luminescent sensing platform was established for tyrosinase (TYR) activity monitoring and its inhibitor screening using one kind of lanthanide coordination polymer nanoparticles AMP–Tb/Ag + . By taking advantage of the specific binding and redox properties of Ag + incorporated into the AMP–Tb network and dopamine (DA) as the product of the model substrate tyramine , the enzymatic reaction and the signal change of the sensing platform was effectively linked. The cooperative effect of a weakened energy transfer from AMP to Tb 3+ by altering the electronic structure of Ag + and an efficient photoinduced election transfer (PET) process caused by dopaquinone facilitated the luminescence quenching of Tb 3+ . Thus, this luminescent sensing platform could be employed for quantitative evaluation of TYR activity. There was a good linear range for TYR activity from 0.08 to 0.20 U mL − 1 with a low detection limit of 0.004 U mL − 1 . Furthermore, this assay was successfully applied to accurate determination of TYR activity in human serum samples and efficient screening of TYR inhibitors. Considering unique spectral characteristics of lanthanides along with operation simplicity and superior analytical performance, this sensing platform is very promising in clinical diagnosis and drugs screening for TYR–associated diseases.
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FOOD CHEMISTRY
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2022
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IF 9.231
Xun Zhou,Yongxiang Wu,Yao Jiang,Chen Li,Longping Xu,Peng Cui,Xinsong She
Integrated one–dimensional CuO–nanowire arrays/Cu–foam (CuO–NWAs/Cu–foam) nanostructure, which was fabricated by oxidation and calcination, has been newly utilized as non–enzymatic electrocatalytic electrode for exploring histamine level. Under optimal condition of pH at 13 and potential at 0.55 V ( vs Ag/Ag/Cl), the sensitivity of CuO–NWAs/Cu–foam electrode towards non–enzymatic electrochemical histamine determination presented as high as 12.94 mA mM −1 cm −2 , linear range spanned between 0.5 and 1046 μM, a detection limit (S/N = 3) was about 44 nM. The unprecedented causes of ultrahigh sensitivity were physically contributed to enhancing active surface area and declining charge transfer resistance. More crucially, the outstanding selectivity, stability and reproducibility facilitated its practical capacity on evaluating histamine levels in different–bacteria fermented mandarin fish, which triggered the potential feasibility for commercializing non–enzymatic electrochemical determination of histamine with high sensitivity, reliable precision and low expenditure.
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JOURNAL OF APPLIED POLYMER SCIENCE
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2023
·Vol. 141
·期 2
·e54792
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IF 3
Qian Chen,Dayong Ren,Weichen Sheng,Kan Zhang
Two novel bio-based benzoxazine monomers containing imide or amide group (HPIDD-fa and HPBA-fa) were synthesized from raw materials of benzoyl chloride/phthalic anhydride, tyramine, furfurylamine and paraformaldehyde via a two-step reaction. Fourier transform infrared spectroscopy (FT-IR), proton and carbon nuclear magnetic resonance (NMR) spectroscopies were performed to characterize the structures of the benzoxazine monomers. The curing behavior was investigated by using differential scanning calorimetry (DSC) and in situ FT-IR, and the DSC results indicate that the peak curing temperatures of HPIDD-fa and HPBA-fa are centered at 248.5 and 230.1°C, respectively. In addition, thermogravimetric analysis (TGA) suggests that the newly developed bio-based benzoxazine-derived polybenzoxazines present excellent thermal stability with T d10 (temperature at 10% weight loss) of 385 and 368°C for poly(HPIDD-fa) and poly(HPBA-fa), respectively. The current investigation suggests that our newly obtained bio-based thermosetting resins are promising materials for high-tech applications.
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Molecular Systems Design & Engineering
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2023
·Vol. 9
·期 1
·86-98
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IF 3.6
Yin Lu,Nan Li,Yaliang Peng,Mohamed Gamal Mohamed,Shiao-Wei Kuo,Kan Zhang
A facile and eco-friendly synthetic strategy has been developed to achieve a series of hydrogen bonding-rich bio-based thermosetting resins in this study. Using both safe and green solvents, we successfully synthesized target bio-based bisbenzoxazines (DcTa-fa, DcTa-sa, and DcTa-da) with high purity from five different naturally resourced raw materials. The chemical structures of the obtained bisbenzoxazine monomers were verified by nuclear magnetic resonance technology (including 1H and 13C NMR, two-dimensional 1H–1H nuclear Overhauser effect spectroscopy (NOESY), and 1H–13C heteronuclear multiple quantum coherence (HMQC)) and Fourier transform infrared spectroscopy (FT-IR). The polymerization processes were systematically investigated by differential scanning calorimetry (DSC) and in situ FT-IR analysis. Contact angle measurements were conducted and the corresponding results revealed tunable surface properties during the polymerization process of each bio-based bisbenzoxazine resin. In order to understand the relationship between the chemical structure and surface properties, more detailed FT-IR analyses were carried out to investigate the hydrogen bonding networks in the resulting polybenzoxazines. Notably, poly(DcTa-fa) presented excellent thermal stability (Td10 of 377 °C, Yc of 53.7 wt%) and strong adhesion strength (5.232 ± 0.26 MPa), while poly(DcTa-sa) and poly(DcTa-da) showed outstanding surface properties with very low surface free energy values (22.91 and 22.84 mJ m−2). These results highlight the utility of smart and sustainable benzoxazine chemistry and offer a facile and green synthetic approach to access hydrogen bonding-rich bio-based benzoxazine resins with many attractive properties.
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CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Yiting Xu,Xiaobo Yu,Meiling Chen,Yi Sun,Wei Zhang,Yajin Fang,Lanyun Fang,Haining Na,Fei Liu,Jin Zhu
Fabrication of multi-responsive fluorescent sensors for real-time and visual monitoring or detection is practical and challenging. Herein, a novel cellulose-based fluorescent functional material with dual-responsive properties arising from two different functional sites was successfully designed and prepared in a CO 2 switchable solvent. Cellulose was effectively dissolved in this solvent and easily functionalized by successively grafting poly (lactic acid) and 7-hydroxy-4-trifluoromethyl coumarin (HFC) onto the cellulose backbone, resulting in C- g -PLLA/HFC with good processibility and fluorescence quenching response (turn off) towards Fe 3+ due to the coordination of Fe 3+ with the carbonate linkage. Acetylation of HFC under microwave-assisted conditions gave rise to C- g -PLLA/HFC-Ac which showed fluorescence enhancement response (turn on) to various organic amines because of the removal of the acetyl group by aminolysis reaction. Therefore, they could be respectively used for selective identification and detection of Fe 3+ , and real-time, accurate and visual monitoring of aquatic product freshness. Furthermore, an interesting application for irreversible information encryption was demonstrated by combining the dual-responsive property. This study suggests a new strategy for constructing cellulose-based functional material with multiple responsiveness and applications.
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FOOD CHEMISTRY
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2023
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IF 8.8
Junjun Wang,Yue Tang,Jia Zheng,Zhengmin Xie,Jianli Zhou,Yuangen Wu
Herein, a colorimetric biosensor for histamine detection in meat is first established based on the enhancement of DNAzyme with peroxidase-mimic activity. Histamine can boost the generation of G-quadruplex sequences, and make them more easily bond with hemin to produce many DNAzyme molecules. In addition, histamine increases the affinity of DNAzyme to the substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Therefore, the obtained DNAzyme can catalyze H 2 O 2 and dissolved oxygen to produce many reactive oxygen species (ROS), which cause the TMB molecule to lose two electrons and generate yellow products, exhibiting a clear absorption peak at 450 nm. The colorimetric biosensor has excellent sensitivity, and the detection limit is as low as 38 μg·L −1 for histamine. Moreover, the biosensor has high selectivity and anti-interference ability, and exhibits a good recovery rate in actual meats. The above results show that the strategy has potential for application in the detection of trace histamine in meats.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Yanan Liu,Jian Chen,Huan Li,Yanbo Wang
Active packaging has been recognized as an effective approach to extend the shelf life of food, but the rapid release of active substances limits the preservation effect. In this study, gallic acid (GA)-loaded ovalbumin (OVA)/chitosan (CS) nanoparticles with slow-release properties were prepared and embedded into the pectin matrix to refine the rapid release of GA in the pectin and elongate the shelf life of salmon fillets. Our results showed that GA could be released continuously from the OVA/CS nanoparticles. The pectin film incorporated with GA-loaded OVA/CS nanoparticles exhibited good light barrier and mechanical properties. The opacity value of the film reached 1.65 ± 0.06 UA/mm, and the tensile strength and elongation at break were 15.97 ± 1.55 MPa and 7.29 ± 0.42 %, respectively. In addition, the pectin film combined with GA-loaded OVA/CS nanoparticles showed improved antibacterial activity against two common biogenic amine-producing bacteria ( Morganella morganii and Escherichia coli ). Moreover, the nanocomposite film delayed salmon fillets' biogenic amine generation, and the shelf life was extended by 3 days compared with the control group. These promising properties supported using the GA-loaded OVA/CS nanoparticle- pectin films as preservation materials for fish.
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