SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Lixia Lu,Bingchan Song,Jianwen Gao,Xinyu Shao
Different environmental estrogens (EEs) may coexist in the same foodstuff, necessitating the establishment of a simultaneous detection method. Although there are numerous studies on the detection of EEs, few was reported on the determination of two or more estrogens at the same time except HPLC . Our efforts have been made to develop a ratiometric aptasensor based on multicolor lanthanide-doped upconversion luminescence nanoparticles (UCNPs) to realize simultaneous and sensitive detection of two EEs (bisphenol A and 17β-estradiol). Three types of core-shell UCNPs with excellent luminous properties and distinct emitting colors were successfully synthesized via doping with various lanthanide ions , two of which were conjugated with specific aptamers and act as energy donors, while another one served as a reference. Because of their excellent luminescence quenching performance, ultra-thin metallic MoS 2 nanosheets were selected as the energy acceptor. Thus, a ratiometric luminescence aptasensor was established and successfully used for the simultaneous detection of two estrogens in tap water and milk samples, demonstrating its considerable practical application value.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Xuan Ma,Yue Wang,Wenting Wang,Jake Heinlein,Lisa D. Pfefferle,Xuemeng Tian
In this study, a facile method has been developed to synthesize a novel type of porous magnetic molecularly imprinted polymers (Fe 3 O 4 -MER-MMIPs) for the selective adsorption and removal of meropenem. The Fe 3 O 4 -MER-MMIPs, with abundant functional groups and sufficient magnetism for easy separation, are prepared in aqueous solutions. The porous carriers reduce the overall mass of the MMIPs, greatly improving their adsorption capacity per unit mass and optimizing the overall value of the adsorbents. The green preparation conditions, adsorption performance, and physical and chemical properties of Fe 3 O 4 -MER-MMIPs have been carefully studied. The developed submicron materials exhibit a homogeneous morphology, satisfactory superparamagnetism (60 emu g −1 ), large adsorption capacity (11.49 mg g −1 ), quick adsorption kinetics (40 min), and good practical implementation in human serum and environmental water. Finally, the protocol developed in this work delivers a green and feasible method for synthesizing highly efficient adsorbents for the specific adsorption and removal of other antibiotics as well.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 8
·3542
·
IF 4.6
Qing Liu,Chao Yan,Yan Wang
Applications of submicron-scale particles are of rising interest in separation science due to their favorable surface-to-volume ratio and their fabrication of highly ordered structures. The uniformly dense packing beds in columns assembled from nanoparticles combined with an electroosmotic flow-driven system has great potential in a highly efficient separation system. Here, we packed capillary columns using a gravity method with synthesized nanoscale C18-SiO2particles having diameters of 300–900 nm. The separation of small molecules and proteins was evaluated in the packed columns on a pressurized capillary electrochromatography platform. The run-to-run reproducibility regarding retention time and peak area for the PAHs using a column packed with 300 nm C18-SiO2particles were less than 1.61% and 3.17%, respectively. Our study exhibited a systematic separation analysis of small molecules and proteins based on the columns packed with submicron particles combined with the pressurized capillary electrochromatography (pCEC) platform. This study may provide a promising analytical approach with extraordinary column efficiency, resolution, and speed for the separation of complex samples.Keywords:submicron scale particles;pressurized capillary electrochromatography;separation
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Metabolism
·
2023
·
IF 29
Di Li,Ting Sun,Yongqing Tong,Juan Le,Qian Yao,Jun Tao,Hang Liu,Woer Jiao,Yufeng Mei,Jingru Chen,Zhongchun Liu,Gaohua Wang,Yan Li
Estradiol decline can result in depressive disorders in females; nevertheless, the causes of this decline are unclear. In this study, we isolated estradiol-degrading Klebsiella aerogenes from the feces of premenopausal females with depression. In mice, gavaging with this strain led to estradiol decline and depression-like behaviors. The gene encoding the estradiol-degrading enzyme in K. aerogenes was identified as 3β-hydroxysteroid dehydrogenase (3β-HSD). Heterologously expressing 3β-HSD resulted in Escherichia coli obtaining the ability to degrade estradiol. Gavaging mice with 3β-HSD-expressing E. coli decreased their serum estradiol levels, causing depression-like behaviors. The prevalence of K. aerogene and 3β-HSD was higher in premenopausal women with depression than in those without depression. These results suggest that the estradiol-degrading bacteria and 3β-HSD enzymes are potential intervention targets for depression treatment in premenopausal women.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2019
·
IF 4.474
Dingding Duan,Xiaojing Si,Yaping Ding,Li Li,Guohong Ma,Lu Zhang,Bingyu Jian
In this paper, we constructed MIL-53 (AlOHbdc, bdc = benzene-1,4-dicarboxylate) /CNTs and Prussian blue (PB) as the double sensitization material of the sensing platform, in which the MIL-53/CNTs hybrid can not only increase the specific surface area but also increase the conductivity of the sensor and PB can play a role in amplifying electrical signals and accelerating electron transmission. Pyrrole was used as monomer and E2 was used as template for electropolymerization to form conductive film. Moreover, the overoxidation/dedoping elution method were used to simplify the experimental process. Under optimal conditions, the MIECS exhibited an excellent sensitivity and high selectivity with a wide linear response range between 10 −14 to 10 −9 mol L −1 and an estimated detection limit of 6.19 × 10 −15 mol L −1 .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Yu Zong,Jia Chen,Jinxuan Hou,Wenwen Deng,Xiaoyan Liao,Yuxiu Xiao
A hexafluroisopropanol (HFIP)-alkyl carboxylic acid high-density supramolecular solvent (SUPRAS) was proposed and combined with dispersive liquid-liquid microextraction (DLLME) for extraction and preconcentration of steroid sex hormones in human urine prior to analysis by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Octanoic acid was used as extraction agent and HFIP, with the properties of high density, strong hydrogen bond donor and high hydrophobicity, was used as dispersing agent of DLLME as well as coacervation-inducing agent and density-regulating agent of octanoic acid . HFIP-octanoic acid SUPRAS was spontaneously formed in the process of DLLME and located in the bottom of a urine solution due to its density higher than water. Thus, the SUPRAS phase at trace volume was easy to be collected after centrifugation using normal centrifuge tubes. Five hormones (four estrogens and progesterone) were chosen as analytes. Urine hydrolysis time, extraction conditions and derivatization conditions for estrogens were optimized. Under the optimal conditions, only 0.07 mL of organic solvent (HFIP) was consumed. An external standard calibration method was used for quantification, and a linear relationship was obtained with correlation coefficients higher than 0.9957 and p values for linear trend less than 0.0001. Limits of detection of the method based on a signal-to-noise ratio of 3 were 0.01-0.10 μg L −1 . The recoveries ranged from 82.7% to 120.2%, and intra-day and inter-day relative standard deviations of the method were less than 15%. The matrix effects of 84.0%–105.7% showed no urine matrix interferences with MS detection, which was mainly due to the restricted access property of HFIP-octanoic acid SUPRAS with reverse micelle aggregates. The absence of matrix effects contributed to the reliable quantitation by external calibration, avoiding the use of expensive isotopically labeled internal standards. The novel SUPRAS-based DLLME method, coupled with HPLC-MS/MS, was applied for determination of target hormones in real urine.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Xinyu Liang,Juntao Wang,Qiuhua Wu,Chun Wang,Zhi Wang
A hypercrosslinked triphenylamine polymer (HCTPA) was prepared through a simple one-step Friedel-Crafts alkylation reaction. The HCTPA had an extended π-conjugated system, large specific surface area and high micropore volume. In order to evaluate its extraction performance, the HCTPA was used as the adsorbent for the solid-phase extraction of the phenylurea herbicides (monuron, chlortoluron, isoproturon, monolinuron and buturon) from watermelon, tomato and cucumber samples. The main parameters that could affect the extraction efficiency including the sample volume, sample solution pH, sample solution loading rate and desorption conditions were investigated and optimized. Under the optimized conditions, the HCTPA showed a good extraction performance for the target analytes. The linear range for the quantification of the analytes was in the range of 0.4–160.0 ng g −1 with the correlation coefficients of 0.9996–0.9999. The limits of detection (S/N = 3) of the analytes by the method ranged from 0.06 to 0.12 ng g −1 for watermelon and tomato, and 0.06–0.15 ng g −1 for cucumber samples. The recoveries of the analytes for the method were between 83.1% and 112.5% with the relative standard deviations below 5.5%. These results indicate that the HCTPA can be used for the enrichment of phenylurea herbicides from complex matrix samples like watermelon, tomato and cucumbers.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Liang-Liang Qian,Rui-Xian Li,Hai-Tao Gao,Chun-Min Wang,Lei Li,Qian Xu,Jian Li
In this work, male rats were exposed to multiple phthalate esters (MIXPs) in a long-term low-dose model for the early evaluation of reproductive toxicity. An ananlysis method with better sensitivity, accuracy and precision was established to determine the five sex hormones (androstenedione, testosterone, dehydroepiandrosterone, dihydrotestosterone, and estrone) in collected urine samples. The results showed that all the analytes in the MIXPs treated group changed in a time-dependent manner. Specifically, estrone significantly decreased from the 30th day and the other four changed from the 30th day and then significantly increased on the 60th day, while no obvious changes were found in the control group. Therefore, a possible way was provided for the early evaluation of male reproductive toxicity induced by Phthalate esters (PEs) . The reliability of judgment was improved by observing the changes of five target hormones simultaneously. Furthermore, good compliance was predicted for the practical application due to the noninvasive and convenient urine sample collection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Guohong Liu,Zhaohan Zhang,Chen Yan,Yang Wang,Xuerui Ma,Peng Gao,Yujie Feng
Boron nitride Nanosheets (BNNSs) was fabricated with a method of heating the mixture of boric acid and urea in N 2 atmosphere and used to remove estrone (E 1 ) from water. The obtained BNNSs exhibited a higher surface area of 896 m 2 /g, a large pore volume of 0.76 cm 3 /g, and only few layers (0.398 nm) with the boric acid and urea ratio of 1:80. The layer number of BNNSs decreased from 15 to 4 with the mole ratio of boric acid and urea decreasing from 1:20 to 1:80, which was identified by SEM , TEM , AFM and BET measurements. More importantly, BNNSs presented an outstanding adsorption performance for estrone with the adsorption capacity of 249.15 mg E 1 /g BNNSs. The adsorption process could be best fitted by pseudo second-order kinetic model and the equilibrium data at different temperatures were well fitted by Langmuir isotherm model. The thermodynamics analysis revealed that E 1 adsorption on BNNSs was spontaneous (ΔG = −29.33 kJ mol −1 ), enthalpy-retarded (ΔH = 29.75 kJ mol −1 ), entropy-driven (ΔS = 198.26 J mol −1 K −1 ), and mostly chemical adsorption. The adsorption rates of E 1 in water were sharply enhanced with thinner BNNSs as absorbents and removal efficiency by BN-60 regenerated after 6 times was above 95%, it was shown that the surface areas, mesopores and remarkable structure played important roles in the adsorption process. The firmness of E 1 onto BNNSs and the stability of adsorption efficiency made BNNSs as a potential absorbent for efficient removal of E 1 from wastewater.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 4
·2857-2864
·
IF 3.201
Chunxi Zhao,Yang Jiao,Lei Zhang,Yaling Yang
Herein, we report a fast and selective technique for the determination of diethylstilbestrol in milk sample using fluorescent sulfur and boron co-doped carbon dots (S,B co-doped CDs). The S,B co-doped CDs were synthesized by hydrothermal synthesis using poly(sodium-p-styrenesulfonate) and borax as precursors. The as-prepared S,B co-doped CDs possess good stability and fluorescence characteristics and exhibit a fluorescence quantum yield of 25.7%. The fluorescence intensity of the S,B co-doped CDs decreased in the presence of diethylstilbestrol, and a good linear relationship existed between the fluorescence quenching and different concentrations of diethylstilbestrol in the range of 0.1–200 μM with a detection limit of 0.06 μM. Finally, the S,B co-doped CDs were successfully applied in the detection of diethylstilbestrol in milk samples, yielding recoveries between 90.4% and 109.2%.
✓ 已验证
产品关联
DOI
高影响因子
WATER AIR AND SOIL POLLUTION
·
2019
·Vol. 230
·期 6
·1-9
·
IF 1.774
Pu Hongbin,Huang Zhibin,Sun Da-Wen,Xie Xiaohui,Zhou Weibiao
Pollution of environmental endocrine disruptors has caused increasing concern globally. In the current study, a simple colorimetric method with high sensitivity and good selectivity for 17β-estradiol (E2) detection was developed, which employed gold nanoparticles (AuNPs) as colorimetric probe for specific recognition of shortening DNA aptamer. Visible color change from bright red to violet was observed for aggregation of AuNPs without the protection of DNA aptamer. After optimization, the method exhibited great performance for E2 detection with good linearity between E2 concentrations from 0.2 to 5 nM and the absorbance ratio at 620 and 523 nm, with the limit of detection of 0.1 nM. The method was also successfully applied to E2 determination in different spiked water samples including fishpond water, lake water, and tap water, in which good recoveries from 93.1 to 108.9% and acceptable relative standard deviations from 3.4 to 8.9% were obtained. This technique showed great potential for on-site fast determination of E2 in environmental water samples.
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Guihua Gao,Sijia Li,Shuo Li,Yudan Wang,Pan Zhao,Xiangyu Zhang,Xiaohong Hou
In this work, computational and experimental methods were used to study the adsorption of estrogens and glucocorticoids on metal–organic frameworks (MOFs). Computer-aided molecular simulation was applied to predict the adsorption of eight analytes on four MOFs (MIL-101(Cr), MIL-100(Fe), MIL-53(Al), and UiO-66(Zr)) by examining molecular interactions and calculating free binding energies. Subsequently, the four water-stable MOFs were synthesized and evaluated as adsorbents for the target hormones in aqueous solution. As the MOF exhibiting the highest adsorption capacity in both computations and experiments, MIL-53(Al) was chosen as a sorbent to develop a dispersive micro-solid-phase extraction procedure coupled to ultra-performance liquid chromatography tandem mass spectrometry for simultaneous determination of the target analytes in water and human urine samples. Experimental parameters affecting the extraction recoveries, including pH, ionic strength , MIL-53(Al) amount, extraction time, desorption time, and desorption solvent, were optimized. The optimized method provided a linear range of 0.005025–368.6 μg/L with good correlation coefficients (0.9982 ≤ r 2 ≤ 0.9992), and limits of detection (S/N = 3) and quantification (S/N = 10) of 0.0015–1.0 μg/L and 0.005–1.8 μg/L, respectively. The analyte recoveries were in the range of 80.6–98.4% in water samples and 88.4–93.2% in urine samples. Furthermore, MIL-53(Al) showed good stability over 10 extraction cycles (RSD < 10.0%). Good agreement between experimental measurements and computational results showed the potential of this approach for elucidating adsorption mechanisms and predicating extraction efficiencies for MOFs and targets, providing new directions for the development and utilization of MOFs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）