MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xin Zhang,Li Tian,Kexin Wu,Zhuo Sun,Qian Wu,Xiangyu Shan,Yingjie Zhao,Ruizhan Chen,Juan Lu
An electrochemical luminescence (ECL) sensor for the detection of glucose was constructed by covering CdTe nanomaterials, ZIF-7 and glucose oxidase (GOD) on the electrode surface, respectively. CdTe and ZIF-7, as co-reaction promoters, could amplify ECL signals through synergy effect. The results demonstrated that ECL intensity increased when the concentration of glucose increased. Based on the change of ECL intensity, a method for the determination of glucose was established. Possible response mechanisms were also studied. The parameters of measurement conditions were optimized. The linear range of glucose was 2.0 × 10 −10 ∼ 2.0 × 10 −5 mol L −1 and the detection limit was 6.7 × 10 −11 mol L −1 under the optimal conditions. The constructed sensor had high sensitivity and selectivity. The results were satisfactory for the determination of glucose in real human serum samples.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Fangfang Yu,Tingya Wang,Yihan Wang,Tengfei Liu,Hongjie Xiong,Liu Liu,Jiang Xiao,Xiaohui Liu,Hui Jiang,Xuemei Wang
It is of great significance to design functional nanomaterials with specific response to tumor microenvironments (TMEs), so as to achieve accurate imaging and efficient treatment of tumor. In this work, a TME-activated biomimetic nanocomposite with dual imaging capabilities of turn-on fluorescence imaging and photothermal imaging was constructed for high-sensitivity sensing of glutathione (GSH) and self-enhanced photothermal/chemodynamic synergistic therapy. The nanocomposite was designed by loading nanozyme MnO 2 and luminol gold nanoclusters on ZIF-8 (Z-M-LA), which was then coated with 4T1 cell membrane (Z-M-LA@CM). The biomimetic membrane decoration was used to escape immune clearance and maintain good biocompatibility and targeting ability. The fluorescence of luminol gold nanoclusters quenched by MnO 2 can be recovered in vivo through MnO 2 degradation by GSH, which enables the TME-activated visualization of tumor. Simultaneously, MnO 2 shows peroxidase-like activity after being degraded by GSH and the released Mn 2+ can catalyze H 2 O 2 to generate abundant hydroxyl radicals (·OH) to realize self-enhanced photothermal/chemodynamic synergistic therapy. Furthermore, Z-M-LA@CM was also investigated to induce immune effect in vivo and it exhibited good pH-responsive degradation behavior, which could remain stable under alkaline conditions (pH = 7.4) and be degraded in acidic solutions (pH = 5.6). These properties make Z-M-LA@CM an appealing biodegradable nanotherapeutic agent for tumor precise diagnosis and therapy.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Bing Yang,Lu Shi,Qiaorong Tang,Wei Liu,Baoxin Li,Chaoyong Yang,Yan Jin
Automated and sensitive detection of nucleic acid is crucial for reliable and safe disease diagnostics because the minimum manual operation can effectively avoid sample contamination and infection risk. Herein, a sensitive, label-free and automated method was developed for detecting trace DNA by integrating digital microfluidics (DMF) with chemiluminescence (CL) detection. DMF can automatically manipulate discrete droplets. Taking advantage of sensitive and simple CL detection, DMF-CL integration can automatically and sensitively detect HIV DNA via the preset pathway. To further improve the sensitivity, on-chip exponential amplification reaction (EXPAR) was explored to produce large quantities of double-stranded DNA (dsDNA) in the presence of HIV DNA. Then, SYBR Green I (SG) intercalated into dsDNA to generate singlet oxygen upon light irradiation , which in situ oxidized potassium ferrocyanide into potassium ferricyanide . Finally, the CL reaction of potassium ferricyanide and luminol ensured the label-free, reliable and specific detection of HIV DNA in the range of 10 pM to 2 nM. Saving time-consuming and laborious manual operations, the DMF-CL integration greatly reduced the reagent consumption and avoided the contamination of DNA sample as well. Therefore, DMF-CL offers a promising and useful technology for developing automated and reliable biosensing and bioanalysis.
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Fundamental Research
·
2021
·
IF 0
Ping Zhou,Shujie Hu,Weiliang Guo,Bin Su
Electrochemiluminescence (ECL) of luminol is a luminescence process that proceeds in the presence of reactive oxygen species (e.g. hydrogen peroxide (H 2 O 2 )) at a suitable electrode potential, the reaction mechanism of which is complicated and remains ambiguous. In this work, we report a visualization approach for measuring the thickness of the ECL layer (TEL) of the luminol/H 2 O 2 system to decipher the reaction process by combined use of the microtube electrode, ECL microscopy, and finite element simulations. With the increase of solution pH, the ECL image captured with the microtube electrode tends to vary from spot to ring, corresponding to the decrease of TEL from >9.1 μm to ca. 4.3 μm. We propose that different intermediates are involved in the course of ECL reaction. At a low pH (e.g. pH < 9), a relatively large TEL is most likely determined by the diffusion of oxidized and deprotonated luminol intermediate that is neutral and has a long lifetime. While at a high pH (e.g. pH in the range of 10 to 12), the ECL reaction is controlled by short-lived radical intermediates of both luminol and superoxide anion. The proposed mechanism is proved theoretically by finite element simulations and experimentally by the apparent effect of concentration ratio of luminol/H 2 O 2 .
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Li-Ping Zong,Junji Li,Guofang Shu,Xinye Liu,Robert S. Marks,Xue-Ji Zhang,Serge Cosnier,Dan Shan
In view of the shortcomings of the current coreactant electrochemiluminescence (ECL) and inspired by natural oxygen (O2) reduction metalloenzymes, a novel ECL amplification strategy was established. A pyrolytic iron- and nitrogen-doped (Fe–N–C) nanosheet rich in singly ionized oxygen vacancy (VO•) defects was rationally designed by destroying the highly saturated coordination with a preorganized ligand 1,10-phenanthroline-2,9-dicarboxylic acid (PDA). Extraordinary catalytic activity for O2 activation was obtained via screening a special pyrolysis temperature using spectroscopic and electrochemical methods. The high-spin ferric centers of highly dispersed FeC nanoclusters and abundant carbon and oxygen vacancy defects fully contributed to the inherent catalytic activity. ECL amplification was achieved by integrating the material with luminol to generate redox-active radicals in situ from dissolved O2 and simultaneously shorten the transferring distance of radicals. Tetracycline (TC), which posed a growing threat to aquatic biodiversity and environmental safety, as a model antibiotic was successfully detected with a detection limit of 3.88 nM (S/N = 3), clarifying a promising application prospect of this new effective ECL amplification strategy in biological analysis and environmental monitoring.
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高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 189
·期 1
·1-10
·
IF 5.833
Liu Guangyan,Li Shiyu,Jiang Zejun,Li Jianping
A novel approach for trace detection of fipronil with a molecularly imprinted electrochemiluminescence sensor (MIECLS) is proposed. The sensitivity is significantly improved via signal amplification of the enzymatic reaction of horseradish peroxidase (HRP) released from encapsulated liposomes which linked onto the template molecules after rebinding. The molecularly imprinted polymer membrane was prepared through the electropolymerization of monomers with fipronil as a template. After the elution of the template molecules, the analyte fipronil was reabsorbed into the cavities. HRP-encapsulated liposomes were linked to the target molecules by light-triggered click reaction. The higher the concentration of the target was, the more HRP-encapsulated liposomes were present on the molecularly imprinted polymer (MIP) sensor. Then, HRP was liberated from liposomes, and the catalytic degradation of hydrogen peroxide (H 2 O 2 ) by HRP occurs, which changed the electrochemiluminescence intensity of luminol significantly. The change of the ∆ ECL intensity was linearly proportional to the logarithm of the fipronil concentration ranging from 1.00 × 10 −14 to 1.00 × 10 −9 mol/L, and the detection limit was 7.77 × 10 −16 mol/L. The recoveries obtained ranged from 95.7 to 105.8% with RSD < 5%. The sensitivity of the detection was significantly improved, and the analysis process was simplified in that the incubation step required in the conventional method was avoided. The sensor proposed provides a feasible platform for ultra-trace amount determination. Graphical abstract
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Guangyan Liu,Jun Ling,Hanzhao Xie,Jianping Li
A molecularly imprinted polymer (MIP)-electrochemiluminescence (ECL) sensor was prepared and an ultrasensitive strategy was proposed for detecting an ultra-trace amount of ethiprole based on the enzymatic signal amplification of glucose oxidase (GOD). The ethiprole molecule on the MIP was marked with GOD-encapsulated liposomes via light-triggered click reaction after elution and readsorption. Many GOD particles were released from the liposomes and catalyzed the reaction to produce a large amount of H 2 O 2 in the presence of glucose, which considerably enhanced the ECL intensity of luminol. Therefore, the sensitivity for ethiprole determination was promoted remarkably. The ECL signal of the sensor was linearly related to the logarithm of ethiprole concentration from 5.00 × 10 −16 M to 5.00 × 10 −11 M, and the detection limit was 1.00 × 10 −16 M, which was the lowest of all the analytical methods used in ethiprole determination. Ethiprole in samples was determined directly without the tedious and complicated sample treatment procedures, such as separation and enrichment. The click chemistry labeling method using liposomes simplified the operation steps of the MIP-ECL sensor in ethiprole determination.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Fuad Abduro Bushira,Shimeles Addisu Kitte,Yong Wang,Haijuan Li,Ping Wang,Yongdong Jin
In this study, an effective oxygen vacancy (Ov)-involved luminol-dissolved oxygen (O2) electrochemiluminescence (luminol-DO ECL) system was developed and exploited for ECL sensing applications through significant plasmon enhancement of the Ov-involved weak luminol-DO ECL signals by the combined use of Cu-doped TiO2 oxygen vacancy and a Au@SiO2 nanomembrane. The results disclosed that the ECL response of the corresponding system could be synergistically boosted, and the plausible underlying mechanism has been discussed. Furthermore, for the first time, the developed system has been successfully applied for the highly sensitive detection of alkaline phosphatase with a low limit of detection of 0.005 U/L, with an excellent linear range from 0.005 to 10 U/L, as well as good stability and reproducibility.
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高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 183
·期 2
·667-673
·
IF 3.741
Xu Shuxia,Li Jialin,Li Xianming,Su Min,Shi Zeming,Zeng Ying,Ni Shijun
This article describes a nonenzymatic chemiluminescence resonance energy transfer (CRET) system for highly sensitive determination of hydroquinone. It was found that the Co(II)-catalyzed luminescence of luminol in pH 11.5 solution is transferred to CdTe quantum dots (QDs) via a CRET mechanism. Hydroquinone is oxidized by H 2 O 2 to benzoquinone which quenches the fluorescence of the QDs. The enhancement is attributed to the adsorption of Co(II) on the surface of the negatively charged QDs. The CRET system was applied in an assay for hydroquinone that has a detection limit of 0.17 nmol L −1 . The detection limit was lower by factors between 100 and 250,000 than earlier reported methods. The assay was successfully applied to the quantitation of hydroquinone in (spiked) water samples, with recoveries ranging from 95 % to 106 %, and relative standard deviations between 0.5 % and 2.4 %.
A Co(II)-luminol-H 2 O 2 -quantum dot chemiluminescence resonance energy transfer system was developed for highly sensitive detection of hydroquinone. Strong signals were observed due to energy transfer between luminol donor and CdTe quantum dots which is catalyzed by Co(II).
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ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Jing Lei,Ling Zhang,Mei Li,Wei Liu,Yan Jin,Baoxin Li
Oxygen vacancy is one intrinsic defect in metal oxide materials. Interestingly, we herein found that the surface oxygen vacancy can significantly enhance the catalytic activity of Co3O4 nanowires in the luminol–H2O2 chemiluminescence (CL) reaction. 0.1 ng/mL Co3O4 nanowires containing 51.3% surface oxygen vacancies possessed ca. 2.5-fold catalytic activity of free Co2+ (the best metal ionic catalyst for the luminol–H2O2 CL reaction). The superior catalytic efficiency is attributed to the enhanced adsorption of H2O2 by surface oxygen vacancies, which in turn accelerates the cleavage of O–O bonds and generates •OH radicals. More importantly, the surface oxygen vacancy-rich Co3O4 nanowires retained about 90% catalytic activity after modification with antibodies. The surface oxygen vacancy-rich Co3O4 nanowires were used to label the secondary antibody, and one sandwich-type CL immunoassay of carcinoembryonic antigen was established. The detection limit was 0.3 ng/mL with a linear range of 1–10 ng/mL. This proof-of-concept work proves that surface oxygen vacancy-rich Co3O4 nanowires are suitable for labeling biomolecules in CL bioanalysis and biosensing.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yanmei Zhou,Shupu Xie,Mingjie Li,Liantao Xin,Xiaoru Zhang,Yi Liu
A CRISPR-Cas12a controlled chemiluminescence resonance energy transfer (CRET) biosensor was constructed for the detection of cancer biomarker carcinoembryonic antigen (CEA). The CRET probe, a single-strand DNA (ssDNA) with hemin/G-quadruplex on one end and CdSe/ZnS quantum dots (QDs) nanosphere on the other end, was used as the substrate for the trans-cleavage of CRISPR/Cas12a system. The hemin/G-quadruplexes catalyzed the oxidation of luminol in the presence of H 2 O 2 . The generating chemiluminescence could excite the CdSe/ZnS QDs encapsulated in the nanosphere, producing CRET. When target CEA specifically identified dual aptamers on the surface of magnetic beads (MBs), the terminal sequence of Aptamer 2 triggered the hybridization chain reaction (HCR), whose product initiated the trans-cleavage activity of CRISPR/Cas12a and destroyed the CRET system. As a result, the luminescence of QDs nanosphere was declined. With HCR, CRISPR-Cas12a trans-cleavage, and mimic DNAzyme catalytic amplification, the biosensor gave a detection limit down to 5.6 pg/mL. Due to the application of CRISPR/Cas12a, the CRET sensor with the characteristics of easy operation and no external light source required was more suitable for point-of-care testing (POCT) detection of various targets.
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Foods
·
2023
·Vol. 12
·期 23
·4363
·
IF 5.2
Zhiping Yu,Chengqiang Li,Jiashuai Sun,Xia Sun,Guodong Hu
The accumulation of pesticide residues poses a significant threat to the health of people and the surrounding ecological systems. However, traditional methods are not only costly but require expertise in analysis. An electrochemiluminescence (ECL) aptasensor was developed using chitosan and molybdenum disulfide (CTS-MoS2), along with acetylene black (AB@CTS) for the rapid detection of malathion residues. Due to the weak interaction force, simple composite may lead to uneven dispersion; MoS2and AB were dissolved in CTS solution, respectively, and utilized the biocompatibility of CTS to interact with each other on the electrode. The MoS2nanosheets provided a large specific surface area, enhancing the utilization rate of catalytic materials, while AB exhibited excellent conductivity. Additionally, the dendritic polylysine (PLL) contained numerous amino groups to load abundant luminol to catalyze hydrogen peroxide (H2O2) and generate reactive oxygen species (ROS). The proposed ECL aptasensor obtained a low detection limit of 2.75 × 10−3ng/mL (S/N = 3) with a good detection range from 1.0 × 10−2ng/mL to 1.0 × 103ng/mL, demonstrating excellent specificity, repeatability, and stability. Moreover, the ECL aptasensor was successfully applied for detecting malathion pesticide residues in authentic samples with recovery rates ranging from 94.21% to 99.63% (RSD < 2.52%). This work offers valuable insights for advancing ECL sensor technology in future applications.Keywords:electrochemiluminescence;aptamer;CTS-MoS2;AB@CTS;PLL-luminol;nano gold
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高影响因子
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