Electrocatalysis
·
2023
·
IF 3.1
Wei Guobing,Fan Qiqi,Hong Nian,Cui Hanfeng,Zhang Wenxing,Rustam Mijit,Alim Alxir,Jiang Tao,Dong Huanhuan,Fan Hao
Here, we developed a novel reagentless aptamer sensor for the detection of aflatoxin B1 (AFB1) that uses a DNA walker strategy and bisferrocene to enhance the signal. First, the special binding of AFB1 and AFB1 aptamer activates the DNAzyme strand. Then, with the aid of Mn 2+ , the DNAzyme strand sequentially cuts the bisferrocene immobilized substrate strand, resulting in a change in the electrochemical signal. And we wrapped Mn 2+ in the organometallic framework UiO-66(Zr)-(COOH) 2 and integrated it with DNA walker. It enables the initiation of the cut reaction without the addition of Mn 2+ during the detection, enabling real-time monitoring. Based on the above advantages, under optimal conditions, the sensor shows good detection linearity in the range of 0.1 pg/mL to 1000 ng/mL, and the detection limit is 4.81 fg/mL. The sensor has also been successfully applied for the detection of AFB1 in complex matrices, including peanut oil samples, with high sensitivity and reagentless operation. In addition, the proposed aptamer sensor can be used to detect other mycotoxins by replacing the aptamer, thus providing an efficient platform for the detection and real-time monitoring applications. Graphical Abstract
A reagentless electrochemical aptamer sensor for AFB1 detection was constructed based on DNAzyme-driven DNA walker and bisferrocene. No significant change in electrochemical signal was observed when the target AFB1 was not added. The electrochemical signal was significantly reduced when the target AFB1 was added.
研究领域:
生物化学
·
食品安全
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Shijin Zhou,Liang Guo,Xinheng Shi,Lele Ma,Huaixia Yang,Mingsan Miao
An electrochemical sensor based on environmentally friendly eRAFT polymerization was developed for the detection of aflatoxin B 1 (AFB 1 ) in food and herbal medicine. Two biological probes, aptamer (Ap) and antibody (Ab), were used to specifically recognize AFB 1 , and a large number of ferrocene polymers were grafted on the electrode surface by eRAFT polymerization, which greatly improved the specificity and sensitivity of the sensor. The detection limit of AFB 1 was 37.34 fg/mL. In addition, the recovery rate was 95.69% to 107.65% and the RSD was 0.84% to 4.92% by detecting 9 spiked samples. The delighted reliability of this method was verified by HPLC-FL.
研究领域:
分析化学
·
食品科学
·
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Yaqi Zhang,Yong Man,Jingna Li,Yaming Sun,Xiuming Jiang,Lijun He,Shusheng Zhang
The aflatoxin B 1 (AFB 1 ) early warning technique based on precursors is an effective strategy for the prevention of AFB 1 contamination risk. The determination of precursors is imperative to ensure the efficiency of the early warning technique. Herein, a controllable magnetic adsorbent Fe 3 O 4 /ZIFs was first introduced for the effective extraction and determination of averantin (AVN) and sterigmatocystin (ST) precursors in cereal by combining magnetic solid-phase extraction (MSPE) and high-performance liquid chromatography (HPLC). Benefiting from the abundant adsorption sites and multifunctional groups matching the analytes , Fe 3 O 4 /ZIFs effectively and simultaneously extracted AVN and ST with great differences in polarity and structure via multiple interactions. AVN was extracted by Fe 3 O 4 /ZIFs mainly through π–π and hydrophobic interactions, while ST was extracted predominantly by electrostatic interactions and surface complexation. The limits of detection were 0.08 μg kg −1 (AVN) and 0.36 μg kg −1 (ST). The developed method exhibited satisfactory spiked recoveries (79.1%–105.4%) in the determination of AVN and ST in rice. This work provides a novel analytical strategy for further studying AFB 1 early warning technique and the formation and transformation of aflatoxins .
研究领域:
分析化学
·
食品安全
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 9
·e70571
·
IF 3.4
Xiaoya Meng,Zhongyu Li,Zili Xia,Dongdong Chen,Zijing Yuan,Yang Han,Heng Zhang,Xia Sun,Jun Seop Lee,Usmanova Surayo Rakhmatzhanovna,Bobokalonov Jamshed Tolehmurodovich,Wenping Zhao
An electrochemical aptasensor based on Ti 3 C 2 T x (MXene) and cDNA-PAA was established for sensitive detection of aflatoxin B1 (AFB1) in peanuts. The high specific surface area of Ti 3 C 2 T x (MXene) contributed to improve electrochemical efficiency and enhancing sensor stability which were characterized by the Randles−Sevcik equation. The self-made polyacrylic acid (PAA) nanogel was added with aptamer complementary chain (cDNA) to form cDNA-PAA nano gel composite, which could realize the synergistic amplification of the difference between the electrochemical signals before and after the addition of AFB1. The sensing effect of cDNA-PAA was validated using DPV. Methylene blue (MB) was modified at the proximal 3′ termini of the aptamer (Apt-MB), meanwhile AuNPs was used to immobilize Apt-MB and improve the efficiency of electrochemical reactions. The competition between AFB1 and cDNA-PAA combined with Apt MB resulted in a significant change in the electrochemical signal current. In addition, the constructed electrochemical aptasensor had a lower detection limit of 1.0 × 10 −3 ng/L for AFB1 under optimal detection conditions, and a detection range of 1 to 1000 ng/L. Moreover, peanuts with different concentrations of AFB1 were used as actual detection samples. Through the constructed sensor detection, the spiked recovery rates within the spiking range of 50 to 500 ng/mL were 97.76% to 101.84% ( n = 3).
研究领域:
生物传感器
·
食品安全检测
·
分子诊断
·
纳米材料应用
·
电化学分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Wenting Li,Yongqiang Shi,Yuanyuan Zhu,Yongjian Yu,Dong Han,Xiaobo Zou,Jiyong Shi
This study developed a novel dual-functional portable electrochemical biosensor capable of simultaneous and sensitive detection of AFB 1 and OTA. The biosensor incorporates SPCE modified with gold nanoflowers, which not only enhances electrical conductivity and specific surface area of work electrode but also supports the stable immobilization of DNA tetrahedral probes. This biosensor utilizes ferrocene and methylene blue as distinct electrochemical signal reporters and enables specific and parallel recognition of AFB 1 and OTA in a single measurement, this strategy greatly improves its detection efficiency. Integrated with a portable electrochemical workstation and smartphone, this platform facilitates rapid, user-friendly, and real-time mycotoxin detection. Under optimized conditions, the biosensor exhibited wide linear detection ranges (0.05–250 ng/mL) and impressive detection limits of 2.8 pg/mL (AFB 1 ) for and 4.1 pg/mL (OTA). This work developed platform exhibits excellent stability and field applicability, providing a practical integrated solution for on-site screening of multiple mycotoxin in agricultural products.
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xueying Gao,Ying Liu,Jinxiang Wei,Zhouping Wang,Xiaoyuan Ma
Aflatoxin B 1 (AFB 1 ) is a virulent metabolite secreted by Aspergillus fungi, impacting crop quality and posing health risks to human. Herein, a dual-mode Raman/fluorescence aptasensor was constructed to detect AFB 1 . The aptasensor was assembled by gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), while the surface-enhanced Raman scattering (SERS) and fluorescence resonance energy transfer (FRET) effects were both realized. AuNPs were modified with the Raman signal molecule 4-MBA and the complementary chain of AFB 1 aptamer (cDNA). MNPs were modified with the fluorescence signal molecule Cy5 and the AFB 1 aptamer (AFB 1 apt). Through base pairing, AuNPs aggregated on the surface of MNPs, forming a satellite-like nanocomposite, boosting SERS signal via increased “hot spots” but reducing fluorescence signal due to the proximity of AuNPs to Cy5. Upon exposure to AFB 1 , AFB 1 apt specifically bound to AFB 1 , causing AuNPs detachment from MNPs, weakening the SERS signal while restoring the fluorescence signal. AFB 1 concentration displayed a good linear relationship with SERS/fluorescence signal in the range of 0.01 ng/mL–100 ng/mL, with a detection limit as low as 5.81 pg/mL. The use of aptamer assured the high selectivity toward AFB 1 . Furthermore, the spiked recovery in peanut samples ranged from 91.4 % to 95.6 %, indicating the applicability of real sample detection. Compared to single-signal sensor, this dual-signal sensor exhibited enhanced accuracy, robust anti-interference capability, and increased flexibility, promising for toxin detection in food safety applications.
研究领域:
食品安全
·
分析化学
·
纳米技术
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Hai Chi,Guoqin Liu
Aflatoxin B1 (AFB1) is a toxic metabolite produced by fungi with substantial environmental and health concerns. Thus, the detection of AFB1 has attracted increasing attention. In this study, a fluorometric sandwich biosensor was developed for the detection of AFB1, which was prepared by combining molecularly imprinted polymer (MIP) with an aptamer. Porous carbon (PC) generated by the carbonization of ZIF-8 provided abundant sites for the MIP layer to achieve high-performance recognition of AFB1. The aptamer-modified CdTe/ZnS achieved efficient sandwich analysis of AFB1 as a signal probe. The sandwich biosensor exhibited excellent selectivity and high sensitivity with a dynamic linear range of 0.01–20 ng/mL and good reliability in spiked edible oil samples as low as 4.0 pg/mL. In sum, the developed sandwich biosensor is promising for the detection of AFB1 in food and the environment, with great potential in the control of other challenging food hazards .
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Xia Yu,He Rui,Sun Ying,Zhou Hangyu,Gao Minjie,Hu Xiuyu,Cui Xiaobing,Cheng Qianqian,Wang Zhouping
Aflatoxins are naturally occurring high-toxic secondary metabolites, which cause worldwide environmental contaminations and wastes of food and feed resources and severely threaten human health. Thus, the highly efficient methods and technologies for detoxification of aflatoxins are urgently needed in a long term. In this work, we report the construction of recombinant Kluyveromyces lactis strains GG799(pKLAC1-Phsmnp), GG799(pKLAC1-Plomnp), GG799(pKLAC1-Phcmnp), and then the food-grade expression of the three manganese peroxidases in these strains, followed by the degradation of aflatoxin B1 (AFB1) using the fermentation supernatants. The expression of the manganese peroxidases was achieved in a food-grade manner since Kluyveromyces lactis is food-safe and suitable for application in food or feed industries. The inducible expression process of the optimal recombinant strain GG799(pKLAC1-Phcmnp) and the aflatoxin B1 degradation process were both optimized in detail. After optimization, the degradation ratio reached 75.71%, which was an increase of 49.86% compared to the unoptimized results. The degradation product was analyzed and determined to be AFB1-8,9-dihydrodiol. The recombinant strain GG799(pKLAC1-Phcmnp) supernatants degraded more than 90% of AFB1 in the peanut samples after twice treatments. The structural computational analysis for further mutagenesis of the enzyme PhcMnp was also conducted in this work. The food-grade recombinant yeast strain and the enzyme PhcMnp have potential to be applied in food or feed industries.
研究领域:
生物技术
·
食品科学
·
微生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Metabolites
·
2023
·Vol. 13
·期 7
·785
·
IF 4.1
Peizhou Yang,Wenjing Wu,Danfeng Zhang,Lili Cao,Jieshun Cheng
Aflatoxin pollution poses great harm to human and animal health and causes huge economic losses. The biological detoxification method that utilizes microorganisms and their secreted enzymes to degrade aflatoxin has the advantages of strong specificity, high efficiency, and no pollution inflicted onto the environment. In this study,Bacillus subtilisWJ6 with a high efficiency in aflatoxin B1degradation was screened and identified through molecular identification, physiological, and biochemical methods. The fermentation broth, cell-free supernatant, and cell suspension degraded 81.57%, 73.27%, and 8.39% of AFB1, respectively. The comparative transcriptomics analysis indicated that AFB1led to 60 up-regulated genes and 31 down-regulated genes inB. subtilisWJ6. A gene ontology (GO) analysis showed that the function classifications of cell aggregation, the organizational aspect, and the structural molecule activity were all of large proportions among the up-regulated genes. The down-regulated gene expression was mainly related to the multi-organism process function under the fermentation condition. Therefore,B. subtilisWJ6 degraded AFB1through secreted extracellular enzymes with the up-regulated genes of structural molecule activity and down-regulated genes of multi-organism process function.Keywords:aflatoxin B1;Bacillus subtilis;microbial degradation;comparative transcriptomics;biological detoxification
研究领域:
微生物学
·
毒理学
·
分子生物学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AOAC INTERNATIONAL
·
2026
·
IF 1.7
Hao Huihui,Wu Mengke,Zhao Xiaolei,Li Jianjun,He Jinxing
BackgroundAflatoxin B1 (AFB1) is widespread in various kinds of food and poses a serious threat to health when it enters the human body via the food chain.ObjectiveThis study aimed to establish a rapid and sensitive method for the detection of AFB1 and then combine it with HPLC for the quantitative analysis of AFB1 content.MethodsIn this work, hydrothermal and sol–gel methods were employed to prepare aminated magnetic nanoparticles featuring strong magnetism and excellent dispersion. Magnetic nanoparticles bound with antibodies can specifically capture AFB1 and then be combined with HPLC for the quantitative analysis of AFB1 content. Moreover, the key factors influencing the extraction efficiency, such as buffer type, adsorption time, elution time and volume, were optimized.ResultsThe samples were spiked with AFB1 at low, medium, and high concentration levels of 5.0, 10.0, and 20.0 μg/kg, respectively. The established method exhibits good linearity within the range of 0.1–100 µg/kg, and the detection limit is as low as 0.09 µg/kg (S/N = 3). The recoveries in real samples ranged from 79.33 to 111.51%, with all RSDs being less than 11.16% (n = 3 for each level).ConclusionIn conclusion, a rapid and efficient analytical method for detecting AFB1 in corn, peanut, and oat based on functionalized immunomagnetic beads (IMBs) coupled with high-performance liquid chromatography with a fluorescence detector (HPLC–FLD) have been successfully developed. The method exhibited excellent sensitivity, featuring a low method detection limit of 0.09 μg/kg, high accuracy, and remarkable precision. In comparison to traditional sample preparation techniques, this IMB-based approach presents substantial advantages in terms of simplicity, rapidity (completing sample processing within approximately 30 min), and cost-effectiveness.HighlightsThe IMBs possess excellent magnetic separation capabilities, which effectively streamlines the pre-processing steps.
✓ 已验证
DOI
高影响因子
Biosensors-Basel
·
2025
·Vol. 15
·期 5
·313
·
IF 5.6
Yinjun Li,Hua Ding,Ziyu Wang,Zewei Luo,Xitian Peng
Lateral flow immunoassay (LFIA) is a promising tool for rapid detection in the field of agricultural product analysis due to its advantages of cost-effectiveness and operational simplicity. In this work, Eu metal–organic frameworks (MOFs) were introduced to LFIA as a rapid detection method characterized by high stability and low interference. Key research objectives included strong fluorescence, ease of labeling, and the utilization of fluorescent probes. Eu-MOFs were synthesized in one step via the hydrothermal method, exhibiting a fluorescence lifetime of 163 μs and spherical particles with diameters ranging from 250 to 400 nm. These conditions fulfill the characteristics and requirements of LFIA. Eu-MOFs exploit the porous nature of MOFs to mitigate the drawbacks associated with complex crosslinking agents. This enables antibody proteins to be cross-linked merely upon contact, thereby simplifying the detection process. A time-resolved LFIA method was developed utilizing Eu-MOFs for the detection of aflatoxin B1 (AFB1) in corn, achieving a limit of detection (LOD, IC10) of 0.149 ng/mL. The accuracy and reliability of the Eu-MOFs-LFIA method were validated through comparisons with spiked concentrations during spiking and blind sample analyses, with verification conducted using ultra-high-performance liquid chromatography mass spectrometry (UPLC-MS). Furthermore, testing of real samples demonstrated that the Eu-MOFs-LFIA method can effectively facilitate rapid detection of AFB1 in corn.
研究领域:
食品科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Meng Wang,Xiaoqi Zhao,Xiaoyun Yang,Xueming He
The fluorescence characteristics of aflatoxin B 1 (AFB 1 ) have enabled the development of an effective and non-destructive screening method for AFB 1 in agro-products using fluorescence spectroscopy. However, the complex and varied matrices present in most foodstuffs can significantly distort the intrinsic fluorescence of AFB 1 . In this study, the absorption and scattering properties of vegetable oils were obtained using double integrating spheres (DIS), and fluorescence intensity was obtained using laser-induced fluorescence (LIF) technique. A six-parameter analytical model has been developed to recover AFB 1 intrinsic fluorescence based on the absorption and scattering features at excitation (375 nm) and emission (424 nm) wavelengths employing a one-dimensional convolutional neural network (1D-CNN). Prediction models for AFB 1 concentration in vegetable oils with gradient variations of matrices were calibrated using the disturbed and recovered intrinsic fluorescence intensity, respectively. The models were validated and compared to demonstrate the feasibility and superiority of the proposed method.
研究领域:
食品安全
·
光谱学
·
人工智能
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）