SENSORS
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2023
·Vol. 23
·期 3
·1362
·
IF 3.9
Haozhi Zhang,Hao Jia,Weiwen Feng,Zao Ni,Pengcheng Xu,Xinxin Li
Ultra-responsive single-crystal silicon MEMS thermopiles for differential thermal analysis (DTA) are developed. Facilitated by a unique “microholes interetch and sealing (MIS)” technique, pairs of suspended thermopiles are batch fabricated in a differential form, with high-density (54 pairs) n-type/p-type single-crystal silicon thermocouples integrated within each thermopile (sample area ~0.045 mm2). The fabricated MEMS thermopile sensors exhibit outstanding power responsivity of 99.5 V/W and temperature responsivity of 27.8 mV/°C, which are more than 4 times higher than those reported for material thermal analysis. The high-responsivity MEMS DTA chips allow us to accurately measure the indium melting point at different heating rates of ~1–100 °C/s. We also perform DTA measurement of the dehydration process of CuSO4·5H2O and the crystals show three stages of losing water of crystallization before becoming anhydrous copper sulfate salt. Our high-performance, cost-effective MEMS sensing chips hold promise for rapid and accurate DTA characterization for a wide range of applications.Keywords:MEMS thermopile;differential thermal analysis;DTA;single-crystal silicon thermocouples;MIS process
研究领域:
材料科学
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微机电系统
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热分析技术
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DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Guowei Wang,Chengpan Li,Chunguang Miao,Shibo Li,Bensheng Qiu,Weiping Ding
With the emergence of various cutting-edge micromachining technologies, lab on a chip is growing rapidly, but it is always a challenge to realize the on-chip separation of living cells from cell samples without affecting cell activity and function. Herein, we report a novel on-chip label-free method for sorting living and dead cells by integrating the hypertonic stimulus and tilted-angle standing surface acoustic wave (T-SSAW) technologies. On a self-designed microfluidic chip, the hypertonic stimulus is used to distinguish cells by producing volume differences between living and dead cells, while T-SSAW is used to separate living and dead cells according to the cell volume difference. Under the optimized operation conditions, for the sample containing 50% of living human umbilical vein endothelial cells (HUVECs) and 50% of dead HUVECs treated with paraformaldehyde, the purity of living cells after the first separation can reach approximately 80%, while after the second separation, it can be as high as 93%; furthermore, the purity of living cells after separation increases with the initial proportion of living cells. In addition, the chip we designed is safe for cells and can robustly handle cell samples with different cell types or different causes of cell death. This work provides a new design of a microfluidic chip for label-free sorting of living and dead cells, greatly promoting the multi-functionality of lab on a chip.
研究领域:
微流控技术
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生物医学工程
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细胞生物学
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芯片实验室技术
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产品关联
DOI
PubMed
高影响因子
Biotechnology Journal
·
2023
·Vol. 19
·期 1
·2300273
·
IF 4.7
Wenqi Hu,Yidan Zhu,Qu Tang,Xiaolei Ji,Lei Wang,Weijun Ou,Guo Li,Li Wu,Hui Cong,Yuling Qin
The chip-based digital polymerase chain reaction (PCR) is an indispensable technique for amplifying and quantifying nucleic acids, which has been widely employed in molecular diagnostics at both fundamental and clinical levels. However, the previous designs have yet to achieve widespread application due to limitations in complex chip fabrication, pretreatment procedures, special surface properties, and low throughput. This study presents a facile digital microfluidic chip driven by centrifugal force for digital PCR analysis. Interestingly, regardless of the hydrophilicity or hydrophobicity of the inner chip surface, an efficient digitization process can be achieved. PCR reagents introduced into the inlet can be allocated to 9600 microchambers and subsequently isolated by the immiscible phase (silicone oil). The centrifugal priming approach offers a facile means to achieve high-throughput analysis. The design was further employed for the quantification of nucleic acids using digital PCR. The calculated result exhibited a strong correlation with the measured value at the concentrations from 1 copy/μL to 1000 copies/μL ( R 2 = 0.99). Additionally, the chip also allowed digital multiplexed analysis, thereby indicating its potential for multi-target detection applications.
研究领域:
分子诊断
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生物技术
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微流控技术
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核酸分析
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Naiqian Zhang,Zhihua Liu,Xiao Yu,Jiaqi Yang,Wu Qiu,Sai Bi,Tianjia Jiang,Dapeng Yang
In situ monitoring of biomarkers in wound fluids using wearable potentiometric sensors is especially significant for early wound infection prediction and effective wound management. However, the antifouling abilities of such sensors in wound fluids have rarely been investigated. Herein, we propose a simple and versatile strategy based on a self-adhesive hydrogel coating to impart antifouling capabilities to a wearable potentiometric sensing chip for on-body wound monitoring. A catechol-functionalized zwitterionic hydrogel is synthesized via a dopamine-triggered gelation strategy, which exhibits a low swellability, good biocompatibility, and high stretchability. Notably, owing to its self-adhesive properties, a thin hydrogel layer could be readily immobilized on various surfaces of the sensing chip without elaborate modification protocols. Using a polymeric membrane-based H+-sensing chip as a model, the hydrogel-coated chip exhibits significantly enhanced antifouling performance and decreased cytotoxicity compared to the pristine one. These improvements are evidenced by the markedly suppressed adsorption of bacteria, proteins, and cells, and an ∼35% increase in cell viability. In situ bacterial infection monitoring in a series of on-body experiments indicates the capability of the proposed antifouling sensing chip to measure both spatial and temporal pH changes in wound microenvironments.
研究领域:
生物医学工程
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可穿戴电子设备
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生物传感器
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伤口护理
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材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Junjie Wang,Chunxiang Xu,Milan Lei,Yi Ma,Xiaoxuan Wang,Ru Wang,Jianli Sun,Ru Wang
The detection of cardiac markers with high sensitivity and specificity is exceedingly essential for the early diagnosis and warning of cardiovascular disease. In this work, we developed a microcavity-based sandwich immune chip to realize the ultrasensitive detection of cardiac troponin I (cTnI) and creatine kinase isoenzyme MB (CK-MB) by surface-enhanced Raman spectroscopy (SERS) method. Herein, polystyrene microspheres modified with gold nanoparticles (Au NPs) were fixed on the substrate via polydopamine, which was used as an immune capture chip. 5,5′-Dithio bis-(2-nitrobenzoic acid) (DTNB) and 4-mercaptobenzoic acid (4MBA) labelled Au NPs served as immune signal probes for cTnI and CK-MB, respectively. Light confinement effect of polystyrene (PS) microcavities and localized surface plasmon resonance (LSPR) near the Au NPs synergistically strengthened the electromagnetic field and promoted the interaction between light and matter, which enhanced SERS performance with an enhancement factor of 1.95 × 10 12 for SERS sensing. The immunoassay results demonstrated the sensitive quantitative detection with the limit of detection (LOD) of 3.16 pg/mL and 4.27 pg/mL for cTnI and CK-MB, separately. The method also had good specificity, reproducibility and stability. It could be expected that our proposed microcavity-based SERS chip immunoassay technique would be considered as an effective diagnostic tool for ultrasensitive detection of cardiac biomarkers.
研究领域:
心脏病学
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纳米技术
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分析化学
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生物医学工程
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DOI
高影响因子
Advanced Science
·
2026
·
IF 14.1
Yu-Chieh Yuan,Beibei Xu,Jenna McCormack,XuHai Huang,Jingzhe Ma,Thomas Marshall,Yacong Sun,Hardeep Singh,Alyssa Fanelli,Gauri Kulkarni,Ji Hye Seo,Paige Gilbride,Bing Wei,Bo Wang,Yanyan Liu,Fei Ma,Lin Zhou,Shuyang Wang,Xiaohua Qian,Zhiyong Xie,Polina Golland,Longlong Si,Yu Shrike Zhang,Xin Xie,Haiqing Bai
Functional precision oncology complements genomic approaches by directly testing treatment options on patient-derived models. However, existing platformssuch as patient-derived xenografts (PDXs) and patient-derived organoids (PDOs), face major barriers in clinical use due to technical challenges, including limited standardization, high costs, long assay times, scalability constraints, and incomplete recapitulation of the patient tumor microenvironment (TME). Here, we present a scalable, low-cost Organ Chip (OC) platform fabricated entirely from thermoplastics via injection molding. Leveraging a patented channel geometry and surface treatment, the device achieves barrier-free hydrogel confinement through capillary pinning without porous membranes, micropillars, or other barrier structures. This automation-compatible platform supports tissue-specific extracellular matrices and co-culture through versatile perfusion modes, with robust imaging compatibility. We demonstrate its feasibility for drug sensitivity testing using multiple cell lines and patient-derived primary cells, with imaging-based phenotypic profiling for accurate quantification of drug responses, closely aligning with clinical outcomes. Additionally, we integrated a deep learning-based image translation model that predicts fluorescence staining from bright-field images. This approach enables longitudinal, label-free phenotypic analysis with higher sensitivity than conventional endpoint staining. Together, this integrated cancer OC system overcomes key technical challenges and offers a promising framework for functional precision oncology through high-throughput, patient-relevant drug testing.
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产品关联
DOI
PubMed
高影响因子
OPTICS EXPRESS
·
2023
·Vol. 31
·期 13
·21212-21224
·
IF 3.8
Zhuo Ren,Chunlan Liu,Yong Wei,Chunbiao Liu,Chen Shi,Xingkai Wang,Yixiong Tang,Rui Wang,Zhihai Liu
The Fiber SPR chip laboratory has become a popular choice in biochemical detection. To meet the needs of different kinds of analytes for the detection range and number of channels of the chip, we proposed a multi-mode SPR chip laboratory based on microstructure fiber in this paper. The chip laboratory was integrated with microfluidic devices made from PDMS and detection units made of bias three-core fiber and dumbbell fiber. By injecting light into different cores of a bias three-core fiber, different detection areas of dumbbell fiber can be selected, enabling the chip laboratory to enter high refractive index detection, multi-channel detection and other working modes. In the high refractive index detection mode, the chip can detect liquid samples with a refractive index range of 1.571-1.595. In multi-channel detection mode, the chip can achieve dual parameter detection of glucose and GHK-Cu, with sensitivities of 4.16 nm/(mg/mL) and 9.729 nm/(mg/mL), respectively. Additionally, the chip can switch to temperature compensation mode. The proposed multi working mode SPR chip laboratory, based on micro structured fiber, offers a new approach for the development of portable testing equipment that can detect multiple analytes and meet multiple requirements.
研究领域:
生物化学检测
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光学传感
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微流控技术
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生物医学工程
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DOI
高影响因子
Smart Medicine
·
2026
·Vol. 5
·期 2
·e70032
·
IF 12.8
Xirui Wang,Xiang Lin,Yue Zhi,Luoran Shang,Yuan Luo,Yongan Wang
Neuroinflammation is a core pathological mechanism in neurodegenerative diseases. Although natural many compounds, derived from traditional Chinese medicine have shown promise in modulating neuroinflammation, conventional evaluation methods remain inefficient and fail to meet modern drug development needs. This study aimed to develop a neuroinflammation-on-a-chip for efficient and accurate evaluation of the anti-neuroinflammatory activity of such compounds. By integrating gelatin methacryloyl (GelMA) hydrogel with a microchamber array structure into a multi-channel concentration-gradient microfluidic chip, we constructed a functional neuroinflammation-on-a-chip suitable for high-throughput drug screening. Preliminary results demonstrated that the chip can successfully model lipopolysaccharide (LPS)-induced neuroinflammation and test the anti-inflammatory effects of curcumin (Cur) and resveratrol (RSV). Relative to traditional approaches, the chip offers the advantages of low sample consumption, rapid detection, and high data reliability. This study provides a novel tool for the efficient evaluation of anti-neuroinflammatory activity of traditional Chinese medicine active compounds and offers an innovative platform for research on neuroinflammation-related diseases. Key Points A neuroinflammation-on-a-chip with a 3D biomimetic microenvironment is developed. It enables high-throughput, dose-dependent drug screening via an on-chip gradient generator. The platform successfully models LPS-induced neuroinflammatory responses. It validates the anti-neuroinflammatory effects of natural compounds Curcumin and Resveratrol.
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Zhishan Liang,Yingming Ma,Ying He,Mengjiao Dai,Dongxue Han
Abnormal dopamine (DA) levels are implicated in numerous physiological disorders—including Parkinson's and Alzheimer's diseases—necessitating highly sensitive and selective analytical methods. Here we report a bismuth tungstate nanoparticle-modified glassy‑carbon electrode (Bi 2 WO 6 NPs/GCE) that enables chronoamperometric DA sensing with excellent electrocatalytic activity. Operating at an oxidation potential of +0.46 V, the sensor responds linearly to DA from 5 nM to 345 μM (R 2 = 0.9997) and offers a detection limit of 2.7 nM. To further miniaturise the platform, Bi 2 WO 6 NPs were drip-coated onto a laser-induced graphene pattern on eco-friendly polyvinyl chloride laser-induced graphene (PVC-LIG), producing a Bi 2 WO 6 NPs/Chip that functions inside a home-built integrated device. The chip requires only 70 μL electrolyte, operates at a lower potential (+0.22 V), and detects DA from 0.5 to 100 μM with a 14 nM detection limit. Both configurations exhibit excellent reproducibility (RSD < 1 %) and have been successfully applied to spike-and-recovery assays in human serum, demonstrating their promise for point-of-care DA monitoring.
研究领域:
分析化学
·
纳米技术
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电化学
·
生物传感器
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神经科学
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生物医学工程
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产品关联
DOI
高影响因子
Science Advances
·
2024
·Vol. 10
·期 46
·
IF 11.7
Wenshang Guo,Ye Tao,Ruizhe Yang,Kaihao Mao,Hongwei Zhou,Minghui Xu,Tie Sun,Xiao Li,Changrui Shi,Zhenyou Ge,Rui Xue,Haizhou Zhou,Yukun Ren
Developing instant detection systems with disease diagnostic capabilities holds immense importance for remote or resource-limited areas. However, the task of creating these systems—which are simultaneously easy to operate, rapid in detection, and cost-effective—remains a challenge. In this study, we present a compact highly sensitive photothermal reverse transcriptase–loop-mediated isothermal amplification (RT-LAMP) chip (SPRC) designed for the detection of multiple diseases. The nucleic acid (NA) amplification on the chip is achieved through LAMP driven by either LED illumination or simple sunlight focusing. SPRC performs sample addition and amplification within a limited volume and autonomous enrichment of NA during the sample addition process, achieving a limit of detection (LOD) as low as 0.2 copies per microliter. Through 120 clinical samples, we achieved an accuracy of 95%, with a specificity exceeding 97.5%. Overall, SPRC has achieved promising progress in the application of point-of-care testing (POCT) by using light energy to simultaneously detect multiple diseases.
研究领域:
分子生物学
·
医学检验
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Wenqi She,Chong Shen,Zaifei Xue,Bin Zhang,Guoliang Zhang,Qin Meng
Current hydrogel strain sensors have never been integrated into dynamic organ-on-a-chip (OOC) due to the lack of sensitivity in aqueous cell culture systems. To enhance sensing performance, a novel strain sensor is presented in which the MXene layer is coated on the bottom surface of a pre-stretched anti-swelling hydrogel substrate of di-acrylated Pluronic F127 (F127-DA) and chitosan (CS) for isolation from the cell culture on the top surface. The fabricated strain sensors display high sensitivity (gauge factor of 290.96), a wide sensing range (0–100%), and high repeatability. To demonstrate its application, alveolar epithelial cells are cultivated on the top surface of the hydrogel strain sensor forming alveolar barriers, and then integrated into dynamic lung-on-a-chip (LOC) systems. This system can sensitively monitor normal physiological breathing, pathological inflammation stimulated by lipopolysaccharide (LPS), and alleviated inflammation through drug intervention.
研究领域:
生物医学工程
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组织工程
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材料科学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2017
·
IF 0.795
Ze-Jie ZHANG,Xi SU,Yi XU,Li CHEN
A multifunctional microfluidic chip integrated with multi-orifice flow fractionation (MOFF) and magnetic capture technique was developed to specifically separate and capture HepG2 cells in artificial samples. The chip contained a glass substrate and a polydimethylsiloxane (PDMS) micro-channel cover plate. The PDMS cover plate consisted of three 10-mm-long injection channels, a MOFF separation zone and a hexagonal cavity cell enrichment detection zone. The MOFF separation zone, with a total length of 20 mm, was consisted of 80 semi-rhombic shrinkage and expansion units with a length of 0.18 mm, a depth of 50 μm, a shrinkage area width of 0.06 mm, and an expansion area of 0.20 mm. The angle between each group of shrinkage and expansion units was 103.0°. In this work, HepG2-blood cell suspension was used as the sample. Based on the principle that the magnetic beads surface modified c-Met antibody could specifically bind to HepG2 cells, an immune-magnetic beads (anti-MNCs) suspension at a concentration of 50 μg mL −1 was prepared by surface carboxylated beads, EDC (1 mg mL −1 ), NHS (1 mg mL −1 ) and c-Met antibody. At the optimized flow rate of 50 μL min −1 , HepG2 in suspension samples was efficiently captured at the detection zone of chip via a magnetic field. The carbon quantum dots were prepared by microwave heating with citric acid and thiourea to label HepG2 cells which made it possible that the captured HepG2 could be visualized in-situ. The captured cells in the chip detection area were counted by microscope. It was shown that the capture rate of HepG2 cells was 88.5% ± 6.7% (500 μL volume contained 10 6 blood cells and 10 HepG2 cells). The results demonstrated the developed multifunctional microfluidic chip may serve as a promising tool for separation and capture of tumour cells.
研究领域:
生物医学工程
·
细胞生物学
·
癌症研究
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DOI
高影响因子
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