NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 18
·10407-10414
·
IF 3.269
Meng Yu,Yongjun Hu,Jianzhi Liu
In recent years, the illegal abuse of β2-adrenergic agonists in livestock for growth promotion has been reported frequently. For conventional immunoassay methods, the development of quantitative and simultaneous detection is a great challenge. In this report, a highly sensitive multiplexed immunoassay based on competitive surface-enhanced Raman scattering (SERS) spectroscopy has been developed for the simultaneous detection of clenbuterol and ractopamine. The SERS nanoprobes based on gold nanoparticles (AuNPs) were attached by 4,4′-dipyridyl (DP), clenbuterol antibody, and 2,2′-dipyridyl (BP), ractopamine antibody respectively. The mode of detection of multiple antibodies was implemented by competitive binding between free clenbuterol, free ractopamine and clenbuterol–BSA, ractopamine–BSA immobilized on the substrates with their antibodies labeled on SERS nanoprobes. The results indicated that compared to conventional methods, the proposed method can detect clenbuterol and ractopamine simultaneously over a wide concentration range (1–1000 pg mL−1) with a lower limit of ca. 1.0 pg mL−1, which holds great potential for applications in biochemical analysis and food safety.
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高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 3
·1089-1097
·
IF 4.831
Zhang Zhihong,Duan Fenghe,He Linghao,Peng Donglai,Yan Fufeng,Wang Minghua,Zong Wei,Jia Chunxiao
A nanocomposite consisting of zinc sulfide quantum dots and polyaniline (ZnSQD@PANI) was placed on a gold electrode along with antibody against clenbuterol to give an amperometric immunosensor for clenbuterol. Compared to the use of pristine PANI, the electrode modified with the ZnSQD@PANI nanocomposite adsorbs clenbuterol antibody much better and therefore exhibits higher sensitivity to clenbuterol. The biosensor, when operated at a working potential of 0.21 V (vs. Ag/AgCl), displays a detection limit as low as 5.5 pg⋅mL −1 and works over the 0.01 to 10 ng⋅mL −1 concentration range. Related species such as salbutamol and ractopamine, urine components such as urea and uric acid, and the ions Ca(II), Na(I), and K(I) do not interfere.
A nanocomposite of zinc sulfide quantum dots and polyaniline was synthesized and utilized as the electrode material for detecting clenbuterol hydrochloride (CH). Sensing includes immunoglobulin G anchoring, adsorption of clenbuterol antibody, and detection of clenbuterol.
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高影响因子
NANO
·
2024
·
IF 1
Ying Zhou,Jinai Bian,Min Tian,Tingting Wang,Guomei Zhang,Yan Zhang,Caihong Zhang
Chronic ingestion of food containing clenbuterol (CLB) in the human body can cause acute poisoning. Thus, it is essential to develop a sensitive CLB detection way. Here, a novel electrochemical aptamer sensor based on aptamer/gold nanoparticles/amino magnetic ferric oxide/graphene nanosheets (aptamer/Au NPs/Fe 3 O 4 –NH 2 /GN) nanocomposite has been developed. The Fe 3 O 4 –NH 2 nanoparticles were self-assembled on GN, and then Fe 3 O 4 –NH 2 /GN nanocomposite was coated on the glassy carbon electrode (GCE) surface. Afterward, Au NPs were electrodeposited on Fe 3 O 4 –NH 2 /GN nanocomposite to fix the aptamer by the Au–S bonds. When CLB was added to the solution, the aptamer will specifically recognize CLB, causing current changes. Under the optimized conditions, the sensor shows better electrochemical performance (linear range is 1 × 1 0 − 1 3 M to 1 × 1 0 − 7 M, the detection limit is 1 . 1 6 × 1 0 − 1 4 M ( S / N = 3 )). It has been successfully applied to the detection of CLB in actual samples. The results show that the aptamer sensor has good selectivity, stability and repeatability, which will provide a novel idea for more sensitive and quicker CLB detection.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yingjie Zhao,Li Tian,Xin Zhang,Zhuo Sun,Xiangyu Shan,Qian Wu,Ruizhan Chen,Juan Lu
A specificity recognition molecularly imprinted electrochemiluminescence sensor (MIECLS) for determination of clenbuterol (CLB) was established by molecular imprinting technology. In this sensing platform, Fe 2 O 3 was used as the carrier to fix Ru(bpy) 3 2+ , and it could also improve electrochemiluminescence (ECL) signal due to large specific surface area and better electronic transmission efficiency. The introduction of molecular imprinted membranes (MIPs) with CLB as template molecules realized the specificity identification of sensors. The intensity of the ECL signal decreased gradually when the concentration of CLB increased. Based on this characteristic, a novel method for measuring CLB was established, and the related mechanism was studied. Under the optimal conditions, the ECL response was proportional to the logarithm of CLB concentration in the range of 1 × 10 −9 mol L −1 to 1 × 10 −4 mol L −1 with a detection limit of 3.3 × 10 −10 mol L −1 . The results prove that MIECLS has great potential in pork and liver samples testing.
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高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Sujun Liu,Kai Shi
The residue of clenbuterol (CLB) in food poses a potential harm to human health. Herein, we presented an electrochemical aptasensor ( E -A-CRISPR) based on employing an aptamer as a specific recognition element and asymmetric CRISPR-Cas12a as signal amplifiers for sensitive, and selective detection of CLB. In this E -A-CRISPR system, the target CLB bound to the aptamer and initiated cascade signal amplification through the DNase activity of CRISPR-Cas12a with two competitive gRNAs. Upon amplification, the active Cas12a cleaved the methylene blue-labeled hairpin probe on the electrode, reducing the peak current. Under optimal conditions, the E -A-CRISPR system showed a wide linear range (1 pM-100 nM) and a low detection limit (500 fM). This system could detect CLB in potable water, pig liver, and pork samples, showing significant potential for food safety monitoring. To our knowledge, this study is the first to use a CRISPR-Cas12a powered system for electrochemical sensing of CLB.
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Chunjie Zhu,Guangying Zhao,Wenchao Dou
A novel immunochromatographic assay (ICA) using colored silica nanoparticles (colored SiNPs) as optical directly-detected label was developed for point-of-care detection of clenbuterol (CLE). To our knowledge, this is the first report that using colored SiNPs as nanolabel to develop an ICA. CLE was selected as the model analyte for competitive ICA. The coloration of monodispersed silica nanoparticles (SiNPs) was preceded in water with the presence of aminosilanes and C.I Reactive Violet 5. The purple SiNPs were introduced into a pre-hydrolyzed Stöber system to regrow a silica shell, forming core-shell purple SiNPs. The silica shell prevented dye on SiNPs from leaking. Under appropriate conditions, the core-shell purple SiNPs based ICA was developed to screen for CLE detection by naked eyes at cut-off values of 3 ng/mL, 6 ng/mL and 5 ng/mL in phosphate buffered saline, urine and pork, respectively. The core-shell purple SiNPs based immunoassay system has no cross activity with other nonspecific structural analogues. The results showed that colored SiNPs are promising to be alternative visible labels for the ICA strip.
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高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Li Wu,Qijie Mo,Shoulian Wei,Ruiping Gao,Shuai Zhang,Xiaohua Zhu
This study demonstrates a novel colorimetric method for clenbuterol (CLB) detection based on the peroxidase-like activity of SiO 2 -coated Fe 3 O 4 nanoparticles (Fe 3 O 4 @mSiO 2 ) modified with montmorillonite (MMT) and sodium dodecyl sulfate (SDS), namely, Fe 3 O 4 @mSiO 2 @MMT-SDS. Fe 3 O 4 @mSiO 2 @MMT-SDS nanoparticles were prepared by modifying Fe 3 O 4 @mSiO 2 with MMT and SDS to impart negative charges to the particle surfaces. The structural properties, steady-state kinetics, catalytic performance, and reaction conditions of the as-prepared nanoparticles were analyzed. Fe 3 O 4 @mSiO 2 @MMT-SDS nanoparticles exhibited excellent dispersion and enzyme-like activity, which improved the affinity with substrate (TMB and H 2 O 2 ) for the catalytic oxidation of CLB by H 2 O 2 , turning the solution blue in the presence of the 3,3ʹ, 5,5ʹ-tetramethylbenzidine chromogen. Under optimal reaction conditions, a strong linear correlation between the absorbance of solution and CLB concentration (R 2 = 0.9983) was observed. Here, the limit of detection, spike recovery, and relative standard deviation were 9.6 × 10 -9 mol · L -1 , 94.2 %–102.2 %, and 2.2 %–4.4 %, respectively. The proposed method is a simple, fast, cost-efficient, and sensitive assay, which can be used to rapidly and accurately screen CLB in pork and animal feeds.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Tao Peng,Jianyi Wang,Sijun Zhao,Yuyang Zeng,Pimiao Zheng,Demei Liang,Ghulam Mujtaba Mari,Haiyang Jiang
A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l -arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor . The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment , qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L −1 . In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L −1 , respectively, with detection limits of 0.003 and 0.023 μg L −1 . Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes , and single-analyte detection.
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PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 7
·1105-1111
·
IF 1.9
Xiaoyun Lin,Yongnian Ni,Xueying Pei,Serge Kokot
An electrochemical biosensor capable of direct detection of DNA damage induced by clenbuterol (CLB) has been developed. A glassy carbon electrode (GCE) was modified using reduced graphene oxide-Nafion and dsDNA to produce a dsDNA/RGO-Nafion/GCE sensor. The modified electrode was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and the results at each stage of the electrode construction were interpreted. They indicated that the electrochemical oxidation peak currents of guanine and adenine at the electrode (dsDNA/RGO-Nafion/GCE) significantly increased as compared with those at the untreated electrode (dsDNA/GCE). Based on this, the sensor was used to measure the change of the oxidation peak currents for guanine and adenine between the intact and damaged dsDNA in the sensor films, and the DNA damage was successfully detected. Furthermore, the plot of peak current decline for the guanine and adenine versus the concentration of CLB was linear in the range of 5.0 × 10−7 to 4.0 × 10−6 mol L−1, with a limit of detection (LOD) of 3.2 × 10−7 mol L−1, which provided a good method to determine CLB indirectly.
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高影响因子
ACS Sensors
·
2024
·
IF 8.2
Jinru Xiao,Jiacheng Ding,Chenyang Sun,Dingbin Liu,Hongmei Gao,Yaqing Liu,Yang Lu,Xia Gao
Sports doping remains a significant challenge in competitive sports. Given that urine analysis is the standard for detecting doping, developing rapid, sensitive, accurate, and high-throughput methods for stimulant detection in urine is crucial. Surface-enhanced Raman scattering (SERS) tag-based immunoassays have emerged as powerful analytical tools known for their high sensitivity and specificity, holding particular promise for stimulant detection in urine samples. However, both the Raman signals of typical SERS tags and sample matrices are within the Raman fingerprint region (<1800 cm–1), which could lead to spectrum overlap, potentially reducing detection accuracy and sensitivity. By recognizing this, we designed a competitive immunoassay that integrates two types of zero-background SERS tags and magnetic separation. These innovative SERS tags exhibit distinctive Raman peaks within the Raman-silent region (1800–2800 cm–1), effectively mitigating potential spectrum overlap with background sample signals. Moreover, magnetic separation not only enhances operational simplicity but also improves the system’s anti-interference capability. Using clenbuterol (CL) and higenamine (HM) as model targets, the SERS-based competitive immunoassay demonstrated sensitive detection of individual CL or HM standards, with limits of detection (LODs) of 0.87 and 0.71 pg/mL, respectively. In multiplex mode, CL and HM can be simultaneously detected with LODs of 1.0 and 0.81 pg/mL, respectively. Furthermore, the recovery rates in urine samples ranged from 83 to 116% (relative standard deviation, RSD ≤ 6.4%) for CL and from 82 to 103% (RSD ≤ 5.1%) for HM, further confirming the reliability of the SERS-based immunoassay for practical applications.
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 412
·期 1
·193-201
·
IF 3.286
Sun Yu,Chen Hui,Ma Pei,Li Junying,Zhang Zheng,Shi Hao,Zhang Xuedian
A highly sensitive approach to detect trace amount of clenbuterol (CB) based on graphene oxide/gold nanoparticles (GO/Au NPs) by surface-enhanced Raman spectroscopy (SERS) was presented. To be specific, the GO/Au nanocomposites were formed by depositing Au NPs onto the surface of GO through an in situ reduction process, where a high density of inherent hot spots was created between Au NPs. By optimizing the depositing density of Au NPs, the strongest electromagnetic coupling effect originating from highly dense hot spots was obtained. The optimized GO/Au was demonstrated to enhance the Raman signals of CB by 4.8 times more than that of CB enhanced by Au NPs. Moreover, GO/Au nanocomposites exhibit good biocompatibility and accessible surface for high adsorption of target molecules through the pi-pi stacking with graphene oxide. Hence, the proposed GO/Au nanocomposites were utilized to capture aromatic molecules like CB and served as excellent sensitive SERS-active substrates for sensing of it, which exhibited an excellent linear performance in the range of 5 × 10 −8 to 1 × 10 −6 mol/L with a limit of detection (LOD) of 3.34 × 10 −8 mol/L (S/N = 3). Due to high-density hot spots with easy operation, this proposed GO/Au nanocomposite–based SERS technique holds great potential in the application of food safety analysis and biomedical science.
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高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Li Liu,Chenghong Long,Shenghui Wei,Yu Sun
The determination of clenbuterol is crucial for food safety and sport science. In this work, we use tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane as cross-linking agents and functional monomers for the electrodeposition of SiO 2 films with the addition of clenbuterol. After the elution process, the prepared electrode can be used for the sensitive determination of clenbuterol due to the molecular imprinting effect. Under optimum conditions, results show that the current of clenbuterol decreases linearly in the range of 2 μM to 0.1 mM, and the detection limit is 31 nM. The proposed electrochemical sensor also exhibits excellent stability and reproducibility, showing its high potential for use in sensing clenbuterol in food samples.
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高影响因子
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