APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2026
·Vol. 110
·期 1
·156
·
IF 4.7
Xu Haoyue,Zhu Xueliang,Li Qian,Liu Yuxi,Wang Qi,Meng Xuelian
Peste des petits ruminants (PPR) is an acute, highly contagious viral disease caused by Peste des petits ruminants virus (PPRV). PPR has inflicted severe economic losses on livestock industries across numerous countries in Africa, Asia, and Europe, posing a significant threat to the survival of endangered wild ruminant species. In this study, we performed prokaryotic expression of the PPRV nucleocapsid (N) protein and prepared rabbit-derived polyclonal antibodies (pAbs). We also identified the characteristics of two mouse-derived monoclonal antibodies (mAbs) against the PPRV N protein. The selected mAb was labeled with horseradish peroxidase (HRP). A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established to detect PPRV antigen using rabbit-derived pAbs and HRP-conjugated mAbs. The threshold for the DAS-ELISA was determined to be 0.227. The DAS-ELISA demonstrated minimum detection limits of 0.195 μg/mL for the recombinant PPRV N protein and 250 50% tissue culture infectious dose (TCID 50 ) for PPRV. Of note, the assay exhibited no cross-reactivity with Capripoxvirus (CaPV), Orf virus (ORFV), Lumpy skin disease virus (LSDV), Foot-and-mouth disease virus (FMDV), or Parainfluenza virus 5 (PIV5). The repeatability of the DAS-ELISA was validated with both intra-assay and inter-assay coefficients of variation (CV%) being less than 9%, indicating good repeatability. Compared to the results obtained using the commercial ID Vet kit (ID-Vet, Montpellier, France) for PPRV antigen detection on the 240 tissue samples, the DAS-ELISA showed a concordance rate of 93.75% and a kappa value of 0.86, demonstrating the reliability of the developed method. The results showed that the DAS-ELISA established in this study is a reliable diagnostic method for the PPRV detection. Key points • Polyclonal and monoclonal antibodies against PPRV N protein were prepared. • A DAS-ELISA based on mAbs and pAbs for detecting virus infection was developed. • The established DAS-ELISA exhibits excellent sensitivity and specificity.
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DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Siyu Wang,Jiayu Xin,Xi Zhao,Changliu He,Cui Li,Lei Zheng,Lin Chen,Xu Zhang
In this study, a high-strength organogel was successfully prepared using a ternary deep eutectic solvent containing choline chloride, glycerol, and aluminum chloride after the pretreatment of lignocellulose (pine wood). This organogel is based on poly(acrylic acid) (PAA) and poly(vinyl alcohol) (PVA). When assembled into a capacitor, the gel electrolyte exhibited an excellent electrochemical performance. This strategy provides a novel recycling method for the high-value utilization of lignin-rich pretreatment waste liquids. The DES-based gel containing lignin forms cross-links between the deep eutectic solvent (DES) and the polymer using lignin as an anchor point through redox reactions, significantly enhancing the mechanical properties of the organogel. The maximum breaking strength reached 0.67 MPa. The highest compressive strength was 1.45 MPa, and the highest compressive modulus was 10.85 MPa. The gel electrolyte was assembled in a supercapacitor, with an ionic conductivity as high as 63.43 mS cm–1. It exhibited a high specific capacitance of 212 F g–1 at a current density of 1 A g–1 and maintained a capacitance retention rate of 71.37% at −40 °C. This study integrates DES in biomass pretreatment and supercapacitor energy storage, enabling high-value use of waste liquid and sustainable resource development. The biomass waste-based method reduces dependence on nonrenewable resources, offering a new sustainable development perspective.
研究领域:
储能技术
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电化学
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高分子科学
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材料科学
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生物质利用
·
可持续能源
·
纳米技术
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yi-Wen Wu,Jie Zhang,Xin-Ran Ma,Jun Cao
An efficient, green, and novel basic deep eutectic solvent (DES)-assisted mechanochemical extraction method, combined with ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, is proposed for simultaneous extraction and determination of target compounds from functional food. Several parameters are investigated using single - factor optimization, and response surface methodology is employed to determine the optimum extraction conditions. The conditions include a grinding time of 316.29 s, 83.72 μL of DES (K 2 CO 3 and glycerol), and vortex time of 2.408 min. The developed method provides good linearity (R 2 > 0.9958), stable intraday and interday precision ranging from 0.07 % to 3.31 %, high sensitivity (LODs <20.83 ng/mL), and acceptable accuracy with recoveries of 83.75 %–102.02 %. Moreover, satisfactory greenness (0.71) and practicality (72.5) are achieved in terms of the Analytical Greenness metric and the blue applicability grade index. The proposed method notably contributes to the simultaneous extraction and detection of flavonoids and saponins in astragali radix.
研究领域:
分析化学
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食品科学
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植物化学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jun Hu,Jing Xu,Yuting Tian,Fangjian Shan,Jiangli Wei,Zixun Chen,Xiaoying Xin,Gaojian Chen,Guodong Fu
Conductive hydrogels (CHs) offer exceptional promise for flexible wearable electronics, however, traditional CHs exhibit poor mechanical, degradability, transparency, and self-healing performance. Moreover, their preparation methods often involve time-consuming processes, reliance on external energy sources, and toxic chemical initiators, retarding their practical application. To address these challenges, a novel nanoparticle-assisted deep eutectic solvents (DES)-catalyzed system was first adopted, achieving the efficient preparation of multifunctional CHs within 10 min by the initiator and crosslinker-free method. The as-prepared hydrogels demonstrating high extensibility (up to 4187 %), elasticity, transparency (>90 %), conductivity (up to 21.5 mS/m), self-healing, resistance to environmental factors (−70 °C, and RT for 14 days), excellent biocompatibility and antibacterial capacity. These properties enable them to realize real-time detection of human strain, voice, and deformation (response time = 210 ms). Furthermore, the hydrogels' inherent anti-inflammatory and antioxidant properties promote efficient wound healing, while their degradability in saline solution (<62 h) aligns with the demands of sustainable wearable technology. Our approach offers a promising avenue for developing environmentally friendly and high-performance CHs that can revolutionize the field of wearable sensors.
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高影响因子
PETROLEUM CHEMISTRY
·
2026
·Vol. 66
·期 1
·49-56
·
IF 1.1
Yu Hongyin,Zhao Rongxiang,Li Xiuping,Hou Liangpei
A deep eutectic solvents (DES) was synthesized by stirring mixture of 1-butyl-pyridinehydrochloride ([C 4 pyr]Cl) and oxalic acid (C 2 H 2 O 4 ) at 100°C. The [C 4 pyr]Cl/C 2 H 2 O 4 DES was characterized by FT-IR and 1 H NMR spectroscopy. This DES is capable of extracting thiophene-type sulfides due to the similar polarity of 1-butyl-pyridine and DBT. The –COOH groups within the [C 4 pyr]Cl/C 2 H 2 O 4 DES are readily oxidized to ‒COOOH by H 2 O 2 , which susequently catalyzes the thiophene oxidation into sulfone in the model oil. The higher polarity of sulfone compared to that of thiophone enhances their solubility in the solvent. When the V(DES)/V(model oil) = 0.2, T = 50°C, and O/S = 6, the DBT removal rate reached 98.6% within 80 min. The catalytic activity of [C 4 pyr]Cl/C 2 H 2 O 4 exhibited only a slight decrease after nine regeneration cycles. The apparent activation energy for DBT removal using [C 4 pyr]Cl/C 2 H 2 O 4 was 33.3 kJ/mol. Experimental results indicate that the low apparent activation energy contributes to the high desulfurization efficiency.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Si Hong,Peng-Fei Wang,Ming-Zhao Xiao,Xiao-Jun Shen,Jia Liu,Xue-Fei Cao,Tong-Qi Yuan
Deep eutectic solvent (DES) pretreatment of lignocellulose is an appealing and green approach for efficient lignin extraction. However, the process of lignin regeneration and DES recovery was time-consuming and labor-intensive. Herein, without any separation and purification after the DES pretreatment of Eucalyptus , the lignin/DES mixture was firstly used to fabricate hierarchical porous carbon materials through DES-thermal carbonization (DTC) and activation. Results revealed that the DTC processing involved degradation, condensation, aromatization, and aggregation, which significantly improved the carbonization degree and thermostability of carbon materials. X-ray photoelectron spectroscopic results confirmed the introduction of nitrogen atom from the choline chloride/lactic acid DES into the fabricated carbon materials during the DTC processing. This phenomenon can generate extra pseudocapacitance and thus increase the specific capacitance of supercapacitors. The capacitance retention of the resultant porous carbon materials (AC-200–12) reached above 95% after 10,000 charge-discharge cycles, which was much superior to commercial activated carbon. This facile and sustainable methodology overcomes traditional complex and expensive methods for preparing carbon materials from hardwood, which show promising potential to be used as electrode materials in a supercapacitor.
研究领域:
材料科学
·
能源技术
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高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Wei Liu,Chuang Jiang,Xiaoyu Li,Haoyu Li,Yu Zhang,Yi Huang,Shangqing Chen,Qingxi Hou
Deep eutectic solvents (DES) have been regarded as green solvents in the biorefinery of lignocellulosic biomass, but long duration time has severely limited efficiency. The microwave-assisted DES pretreatment along with enzymatic hydrolysis and high-pressure homogenization process was proposed to produce lignin-containing cellulose nanofibrils (LCNF) from corncob. Benefiting from microwave-assisted DES pretreatment, the duration time was greatly shortened; meanwhile the effects of different kinds of DES on the resultant LCNF were investigated. The results showed that, the microwave-assisted DES fabricated LCNF (M-LCNF) was successfully obtained, exhibiting good nano size, thermal stability , colloidal stability, and fluorescence. M-LCNF was further introduced into phytic acid (PA) enhanced poly(acrylamide- co -acrylic acid) (P(AM-co-AA)) network and constructed composite conductive hydrogels (PLP). The obtained hydrogels exhibited good mechanical strength , UV blocking ability, fluorescence, and conductivity. A simple battery assembled with the resultant PLP as electrolyte had an out voltage of 2.41 V. The composite conductive hydrogel showed good sensing performance towards different stimuli (e.g., stretching and compression) and human motions in real time. It is expected that this research would provide an alternative way for green fabrication of LCNF and potential application of LCNF in flexible sensors.
研究领域:
材料科学
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纳米技术
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生物炼制
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复合材料
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可持续化学
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聚合物科学
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生物医学工程
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DOI
PubMed
高影响因子
CELLULOSE
·
2023
·Vol. 30
·期 17
·10953-10969
·
IF 5.7
Xie Hongxiang,Fan Yutong,Lu Dexiang,Yang Hongbin,Zou Yang,Wang Xumei,Ji Xingxiang,Si Chuanling
In this study, the zwitterionic cellulose nanofibers (Z-CNFs) were prepared by pretreating bleached softwood kraft pulp (BSKP) with a deep eutectic solvent (DES) composed of betaine and anhydrous oxalic acid and then high-pressure homogenization. Some carboxyl groups from oxalic acid and quaternary ammonium groups from betaine were introduced onto the surface of cellulose by esterification in this pretreatment process, which endows cellulose nanofibers (CNFs) with zwitterionic characteristic. A significant feature is that the zeta potential of as-prepared CNFs approaches − 4 eV, so that Z-CNFs are prone to aggregation. The Z-CNFs maintain cellulose Iβ crystal form with a width of 5–15 nm and a length of > 1 μm, and demonstrate good thermal stability ( T on = 188–190 °C, T max = 359–363 °C). The steady rheological analysis and modulus analysis show that all Z-CNF suspensions are pseudoplastic fluid with shear-thinning behavior, and have weak fibril network structure but still exhibit solid like behavior under low shear conditions at a concentration of 1.2%. The Z-CNF films fabricated from Z-CNFs exhibit good light transmittance (76–83%), high haze (87–90%) and high tensile strength (96 MPa). Therefore, an efficient green pretreatment method was developed for preparation of zwitterionic cellulose nanofibers, which could be used in the field of various fluid and film materials.
研究领域:
材料科学
·
纳米技术
·
化学工程
·
胶体与表面化学
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DOI
高影响因子
Talanta Open
·
2025
·
IF 3.7
Xin Li,Kaigang An,Shuang Zhang,Jing He,Huifeng Liu,Hongdeng Qiu,Yiqun Wan,Jia Chen
Rapid detection of antibiotic residues in food is essential for public health. This study aims to develop a highly sensitive colorimetric sensing platform based on a novel nanozyme. To this end, we used a green, tunable, and safe choline chloride/urea-based deep eutectic solvent (DES) to synthesize a metal organic framework, Cu-BTC (BTC: 1,3,5-benzenetricarboxylate), with many highly dispersed copper active sites. As a result, the prepared Cu-BTC showed strong peroxidase-like activity. In the presence of hydrogen peroxide (H 2 O 2 ), it efficiently catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue product TMBox. Importantly, we found that tetracycline (TC) specifically and significantly inhibits this color reaction. The inhibition occurred because TC can directly reduce the blue TMBox, suppressing color development. Based on this effect, we developed a new colorimetric method for sensitive TC analysis. The method had a wide linear range (0.025-25 μM) and a very low detection limit (0.0024 μM). Moreover, it was successfully applied to accurately detect TC in complex food matrices such as milk and honey. Thus, this work not only provides a green route to synthesize high-performance nanozymes, but also establishes a simple, fast, and sensitive colorimetric platform for on-site detection of antibiotic residues in food.
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高影响因子
RSC Advances
·
2019
·Vol. 9
·期 25
·14400-14406
·
IF 3.049
Rong-xiang Zhao,Xiu-ping Li,Chun-feng Mao,Liangpei Hou,Xiaohan Gao
N,N-Dimethylformamide hydrochloric acid/XMCln ([HDMF]Cl/XMCln, M = Zn or Fe, n = 2 or 3) was synthesized by stirring the mixture of [HDMF]Cl and metal chloride. [HDMF]Cl-based DES was characterized by FT-IR spectroscopy, ESI-MS and 1H-NMR spectroscopy. The oxidative desulfurization activity was investigated using [HDMF]Cl/0.2FeCl3 and [HDMF]Cl/ZnCl2 as the extractant and catalyst, and hydrogen peroxide (H2O2) as the oxidant. The desulfurization rate can reach up to 98.08% and 99.2% for DBT using [HDMF]Cl/0.2FeCl3 and [HDMF]Cl/ZnCl2, respectively. After recycling for 7 times, the removal rate of DBT still can reach more than 97%.
研究领域:
化学工程
·
环境科学
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DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2022
·
IF 4.05
Nie Kai,Liu Shaoyang,Zhao Tao,Tan Zhijian,Zhang Yuanming,Song Yan,Li Boya,Li Linlin,Lv Wanwan,Han Guangting,Jiang Wei
Efficient fractionation and valorization of biomass major components are important for biomass utilization. Deep eutectic solvent (DES) treatment is a promising method for biomass processing. In this study, a choline chloride oxalic acid dihydrate (ChCl-OA) DES treatment method was developed to efficiently separates biomass components and isolate the lignin from bamboo. Hemicellulose (from 16.2 to 3.2%) and lignin (from 27.4 to 4.9%) components in recovery solid were significantly reduced. Results revealed that the DES-extracted lignin (DESL) had a higher yield (82.1%) and purity (94.9%), but a lower molecular weight (1148 g/mol) compared with the milling wood lignin (MWL) and alkali lignin (AL). Meanwhile, the DESL from the acid DES pretreatment of bamboo biomass was beneficial to the preparation of stable and homogenous lignin nanoparticles (LNPs). This study provides an efficient method to separate the main components of biomass and prepare LNPs. The DES treatment showed great potential for biomass component fractionation and lignin valorization.
研究领域:
生物化学
·
材料科学
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Gul Muhammad,Jingjing Wang,Wenlong Xiong,Yongkun Lv,Shen Zhang,Anqi Zhao,Parisa Jahanbakhsh-Bonab,Alexei Solovchenko,Jingliang Xu,Md. Asraful Alam
The intention of the present study was to develop a green and sustainable extraction procedure to obtain lutein from microalgae. A series of acidic and polyol based deep eutectic solvents (DESs) were synthesized to establish a novel pretreatment process. Response surface methodology (RSM) was adopted to analyzese the significance of pretreatment parameters with high prediction levels of lutein yield. Amongst all the DESs-assisted pretreatment, polyol based ChCl:Gly (1:2) DES obtained the maximum lutein yield at the following optimal conditions: biomass-to-DES ratio, 3:34.6; temperature, 26 °C; time, 40 min. Lutein extraction was optimized by one-factor-at-a-time approach, and results showed that 1.35 mg/g lutein could obtained at 40 °C for 20 min with a biomass-to-solvent ratio of 30 mg/mL. The recyclability of the DES ChCl:Gly (1:2) was also tested and showed satisfactory results up to three cycles. The mechanism of lutein extraction using ethyl acetate was explored by molecular dynamics.
研究领域:
化学工程
·
生物技术
·
可持续化学
·
提取技术
·
微藻生物技术
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DOI
高影响因子
危险品化学品经营许可证（带存储）