COLLOIDS AND SURFACES B-BIOINTERFACES
·
2014
·
IF 4.287
Shuai Li,Qin He,Tianchan Chen,Wei Wu,Kening Lang,Zhong-Ming Li,Jianshu Li
The combination of multiple drugs within a single nanocarrier can provide significant advantages for disease therapy and it is desirable to introduce a second drug based on host–guest interaction in these co-delivery systems. In this study, a core-stabilized mixed micellar system consisting of β-cyclodextrin-conjugated poly(lactic acid)- b -poly(ethylene glycol) (β-CD-PLA-mPEG) and DL-Thioctic acid (TA) terminated PLA-mPEG (TA-PLA-mPEG) was developed for the co-delivery of DOX and fluorescein isothiocyanate labeled adamantane (FA). DOX can be loaded within the hydrophobic segment of PLA and FA may form stable complexation with β-CD in the core. The mixed micelles (MM) are based on well-accepted medical materials and can be easily cross-linked by adding 1,4-dithio- d , l -threitol (DTT), which can enhance the stability of the system. Drug-loaded MM system was characterized in terms of particle size, morphology, drug loading and in vitro release profile. Cytotoxicity test showed that blank MM alone showed negligible cytotoxicity whereas the drug-loaded MM remained relatively high cytotoxicity for HeLa cancer cells. Confocal laser scanning microscopy (CLSM) demonstrated that the MM could efficiently deliver and release DOX and FA in the same tumor cells to effectively improve drugs’ bioavailability. These results suggested that the core-stabilized MM are highly promising for intracellular co-delivery of multiple drugs.
研究领域:
药剂学
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纳米医学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Photodiagnosis and Photodynamic Therapy
·
2022
·
IF 3.577
Junting Tao,Hao Zou,Xiaokun Liao,Xinjian Lu,Jun cao,Jiaqi Pan,Chaorong Li,Yingying Zheng
Green fluorescent carbon dots (CDs) were prepared by one-step hydrothermal method and then modified into folic acid functionalized carbon dots (FA-CDs) and hyaluronic acid functionalized carbon dots (HA-CDs) with targeted function to study their application in breast cancer cells imaging. The microstructure of the CDs observed through TEM showed the CDs with a scale of 2.69 nm. FT-IR and XPS showed the changes of bonds and functional groups that confirmed the transformation of COOH and NH 2 to amide bonds. FA-CDs and HA-CDs had good water solubility and cytocompatibility, which laid a foundation for their application in human breast cancer cells imaging. At the same time, FA-CDs and HA-CDs had strong fluorescence excitation, and the optimal emission wavelength was about 450 nm. In fluorescence imaging of cells, carbon dots had bright green fluorescence in both breast cancer cells (MCF-7 cells) and normal cells (EC cells). After targeted endocytosis, FA-CDs and HA-CDs could emit bright green fluorescence in cancer cells but could not in normal cells, which proved that the synthesized FA-CDs and HA-CDs had targeting properties. FA-CDs and HA-CDs could be used to accurately identify breast cancer cells and normal cells as cancer diagnosis material, which had the potential application in early cancer diagnosis.
研究领域:
纳米技术
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生物医学工程
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癌症研究
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Oncology
·
2021
·
IF 6.244
Liang Dongxin,Tuo Xiaoqian,Wang Qing,Zhao Lanbo,Zhang Kailu,Wang Yiran,Feng Xue,Yin Panyue,Guo Lin,Liu Yu,Wang Lei,Han Lu,An Ruifang,Li Qiling
The lack of cytopathologists delays the advancement of screening for endometrial cancer. It was urgent to develop a new dye for rapid diagnosis. Our study aimed to synthesize a targeted folate receptor-α near-infrared (NIR) fluorescent agent, folic acid-zwitterionic NIR fluorophore (ZW-FA), and explore the feasibility for screening of endometrial cancer and precancerous change. Folic acid was conjugated with zwitterionic NIR fluorophore. The preparation of ZW-FA was validated by 1H NMR, mass spectrometric, ultraviolet spectra and fluorescence spectra. ZW-FA was incubated with endometrial cytology samples obtained from patients who underwent dilation and curettage or total hysterectomy. Diagnostic utility was calculated by applying laser confocal microscope, Image-J and statistical models, such as enumeration, receiver operating characteristic curve, logistic regression, support vector machine and decision tree were used. The purity of ZW-FA was > 95% determined by 1H NMR. ZW-FA had the strongest absorption peak at 633 nm in ultraviolet spectra. Photostability of ZW-FA was over 8 hours. In clinical validation, a total of 92 patients were enrolled. The cut-off value of ZW-FA was 49 in enumeration, which was used to distinguish the type of samples. Indicators about diagnostic utility are as follows: sensitivity 90.77%, specificity 62.96%, false-positive rate 37.04%, false-negative rate 9.23%, positive predictive value 85.51% and negative predictive value 73.91%. The samples processed by ZW-FA did not affect further Hematoxylin-Eosin staining and pathological diagnosis. It was an effective cytologic strategy for in vitro diagnosis of endometrial cancer and precancerous change by using ZW-FA.Clinical Trial Registration: http://www.chictr.org.cn/index.aspx, identifier ChiCTR1800020123.
研究领域:
肿瘤学
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医学影像学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Communications
·
2026
·
IF 4.9
Xinxu Wang,Aoxue Zhang,Xurui Wang,Xiaohua Yang,Kaixin Bi,Yanan Gao,Lunhang Liu,Yan Lei,Binyue Zhang,Canzhou Wang,Yanhong Wang,Hongyan Jia
Malignant tumors remain a major global health burden due to high mortality and limited therapeutic efficacy. Nanomaterials offer promising strategies for cancer treatment owing to their tunable physicochemical properties. In this study, a multifunctional MIL-101@ICG@FA nanocomposite was constructed to integrate photothermal, photodynamic, and chemodynamic therapies. The Fe-based MOF exhibited high porosity and loading capacity, enabling effective incorporation of indocyanine green. The nanoplatform displayed strong photothermal conversion, enhanced reactive oxygen species generation, and Fenton-like catalytic activity under near-infrared irradiation. In-vitro assays demonstrated potent cytotoxicity against tumor cells, while in-vivo experiments confirmed efficient tumor growth inhibition with no obvious toxicity observed in the short-term evaluation. These findings highlight the synergistic therapeutic advantages of MIL-101@ICG@FA and support its potential as a promising candidate for multimodal cancer therapy.
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry A
·
2026
·
IF 9.2
Miaosen Zhang,Jintian Li,Weiwei Mao,Wenjun Zhang,Liang Chu
Metallic lead (Pb0) defects in halide perovskites act as deep-level traps, serve as charge-recombination centers, and accelerate crystal structure degradation. Here, blade coating under ambient air conditions is employed to scalably fabricate FA–Cs perovskite films for inverted photovoltaic modules. During this process, (CH3)3SI is incorporated to interact with the surplus PbI2, resulting in the formation of a one-dimensional (1D) (CH3)3SPbI3 phase and suppressing harmful Pb0 defects. The 1D phase can optimize energy-level alignment, reduce defect states, inhibit carrier recombination, and enhance carrier transport. By optimizing the (CH3)3SI concentration, the power conversion efficiency of perovskite modules on a 10 × 10 cm2 substrate is enhanced from 18.25% to 19.98%, accompanied by improved open-circuit voltage and fill factor. Besides, the hysteresis index is reduced from 0.09 to 0.02. Additionally, the unencapsulated modules retain approximately 90% of their initial efficiency after 1000 hours, whereas the control devices maintain only 84% after 740 hours.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Weijun Fang,Wenjuan Zhu,Hu Chen,Hanyuan Zhang,Shi Hong,Wenmei Wei,Tingting Zhao
Developing of multifunctional nanoplateforms for simultaneous accurate cancer detection and target therapy is essential in cancer treatment. Herein, we present a facile synthesis of DOX/MNPs-FA nanocomposites for high-performance MRI and tumor-targeted drug delivery. In such core–shell structured DOX/MNPs-FA nanocomposite, the high magnetism of iron oxide core can be tuned and achieved by controlling the Zn2+ dopant amount, making them suitable as excellent contrasts in T2-weighted MR imaging. The outer porous silica shell with large pores about 5.4 nm in diameter possesses high surface area and pore volume. Anticancer drug (DOX molecules) can be stored in the large pore channels and is triggered to be released under acidic condition (pH 4–6). Importantly, the presence of folic acid on the surface of DOX/MNPs-FA allows the targeted delivery of the DOX to tumor cells, and therefore improves tumor chemotherapy efficiency. Our in vitro studies also demonstrated that DOX/MNPs-FA could be efficiently internalized into HeLa cells via the folate receptor-mediated endocytosis, and generate a greater cytotoxicity toward cancer cells compared to free DOX and DOX/MNPs. Therefore, these DOX/MNPs-FA multifunctional nanocomposites have great potential applications for simultaneous tumor diagnosis and targeted chemotherapy.
研究领域:
生物医学工程
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癌症治疗
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材料科学
✓ 已验证
产品关联
DOI
PubMed
Small
·
2017
·Vol. 13
·期 23
·1700484
·
IF 8.643
Ning Zhou,Yiheng Shen,Yu Zhang,Ziqi Xu,Guanhaojie Zheng,Liang Li,Qi Chen,Huanping Zhou
Engineering the chemical composition of organic and inorganic hybrid perovskite materials is one of the most feasible methods to boost the efficiency of perovskite solar cells with improved device stability. Among the diverse hybrid perovskite family of ABX 3 , formamidinium (FA)-based mixed perovskite (e.g., FA 1− x Cs x PbI 3 ) possesses optimum bandgaps, superior optoelectronic property, as well as thermal- and photostability, which is proven to be the most promising candidate for advanced solar cell. Here, FA 0.9 Cs 0.1 PbI 3 (Cl) is implemented as the light-harvesting layer in planar devices, whereas a low temperature, two-step solution deposition method is employed for the first time in this materials system. This paper comprehensively exploits the role of Cs + in the FA 0.9 Cs 0.1 PbI 3 (Cl) perovskite that affects the precursor chemistry, film nucleation and grain growth, and defect property via pre-intercalation of CsI in the inorganic framework. In addition, the resultant FA 0.9 Cs 0.1 PbI 3 (Cl) films are demonstrated to exhibit an improved optoelectronic property with an elevated device power conversion efficiency (PCE) of 18.6%, as well as a stable phase with substantial enhancement in humidity and thermal stability, as compared to that of FAPbI 3 (Cl). The present method is able to be further extended to a more complicated (FA,MA,Cs)PbX 3 material system by delivering a PCE of 19.8%.
研究领域:
材料科学
·
能源技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CURRENT PHARMACEUTICAL DESIGN
·
2022
·Vol. 28
·期 9
·743-750
·
IF 3.31
Ning Zhouyu,Yang Lina,Yan Xia,Wang Dan,Hua Yongqiang,Shi Weidong,Lin Junhua,Meng Zhiqiang
Background: To investigate the effects of the Lenvatinib@H-MnO2-FA administration system on the proliferation and apoptosis of Intrahepatic cholangiocarcinoma (ICC) and the underlying molecular mechanism. Materials and Methods: In this research, hollow MnO2
(H-MnO2) was synthesized via the modified Stöber method, and H-MnO2 was modified with polyethylene glycol-bis (Amine) (NH2-PEG-NH2) and folic acid (FA) to obtain H-MnO2-PEG-FA (H-MnO2-FA).
Lenvatinib was coated in the hollow cavity of H-MnO2-PEG-FA to further form a nanometre drug-carrying system (Lenvatinib@H-MnO2-PEG-FA). Lenvatinib@H-MnO2-FA was characterized through transmission electron microscopy (TEM)
and scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FT-IR) was used to verify that Lenvatinib was loaded on nanoparticles. Functionally, confocal laser scanning microscopy (CLSM), 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) staining, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay were performed to determine the effect of Lenvatinib@H-MnO2-FA on the proliferation and apoptosis of ICC cells (9810 cells). Finally, the protein levels of Raf-1MEK1/2-ERK1/2 signalling pathway components were detected through Western blotting analysis.
Results: We successfully synthesised a Lenvatinib@H-MnO2-PEG-FA administration system. The resulting nanomaterials had excellent biological stability and improved targeting effects. Functionally, Lenvatinib@ H-MnO2-FA inhibited the proliferation
of 9810 cells. The Bcl-2 protein level was significantly downregulated, and the caspase-3 protein level was significantly upregulated, indicating that Lenvatinib@H-MnO2- PEG- FA promoted the apoptosis of 9810 cells. Mechanistically, Lenvatinib@H-MnO2-FA
increased the phosphorylation levels of Raf, MEK1/2, and ERK1/2. Conclusion: H-MnO2-FA can more effectively deliver Lenvatinib to inhibit proliferation and promote apoptosis in ICC, which could be the promising drug delivery nano-vehicles for delivery drugs.
研究领域:
药理学
·
肿瘤学
·
纳米技术
·
细胞生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2020
·
IF 2.734
Xiaojie Song,Kangli Lan,Qi-Yan Lv,Hongmin Liu,Hui-Fang Cui
Design and fabrication of multifunctional nano-carrier is a promising tool in the field of nanomedicine for glioma theranostics. In this study, blue color nanoparticles with targeted tumor cell staining and drug delivery capabilities were simply fabricated by crosslinking lysine (Lys) and lysine-folic acid (Lys-FA) mixture with genipin as the crosslinking agent through a reversed microemulsion method. Doxorubicin (DOX) as the model drug was loaded through electrostatic interaction. The nanoparticles before (Lys-FA NPs) and after (Lys-FA/DOX NPs) loading of DOX were characterized by ultraviolet–visible (UV–Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), dynamic laser light scattering (DLS), transmission electron microscopy (TEM), and fluorescence spectroscopy. Ultimately, the glioma cell staining ability of Lys-FA NPs and cytotoxicity of Lys-FA/DOX NPs were tested in vitro . The results showed that the particle size of Lys-FA NPs can be tuned by adjusting the ratio of Lys to Lys-FA simply. The Lys-FA NPs exhibited dark blue and the blue color is stable under different pH values and temperatures. The feature makes the staining glioma cell pellets visible to naked-eyes. The model drug DOX was loaded on Lys-FA NPs indicated by the increase of size and zeta potential , and change of optical properties. The DOX loaded on the Lys-FA NPs can be sustainedly released, other than burst release. Encouragingly, the Lys-FA NPs can deliver DOX into cells and dramatically reduce cell viability, as proved by U87 cell cytotoxicity analysis and confocal fluorescence imaging. In addition, the Lys-FA NPs (≤2.5 mg mL −1 ) are nontoxic to CHO cells. In a short, the blue color Lys-FA NPs can visibly stain glioma cell pellets and deliver DOX into glioma cells, which can potentially be applied as a multifunctional nanocarrier for tumor imaging and drug delivery.
研究领域:
纳米医学
·
癌症研究
·
药学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-12
·
IF 5.228
Yin Ting,Huang Peng,Gao Guo,Shapter Joseph G.,Shen Yulan,Sun Rongjin,Yue Caixia,Zhang Chunlei,Liu Yanlei,Zhou Sui,Cui Daxiang
The development of targeted nanoprobes is a promising approach to cancer diagnostics and therapy. In the present work, a novel multifunctional photo/magnet-diagnostic nanoprobe (MNPs-PEG 2K -FA@Ce6) has been developed. This nanoprobe is built using folic acid (FA), bifunctional polyethylene glycol (PEG 2K ) and photosensitizer chlorin e6 (Ce6). The MNPs-PEG 2K -FA@Ce6 nanoprobes are superparamagnetic, can be synthesized on a large scale by a one-pot hydrothermal process without further surface modification and are stable in an aqueous environment for eight months. Compared with free Ce6 nanoprobes in vitro studies, the MNPs-PEG 2K -FA@Ce6 nanoprobes significantly enhance cellular uptake efficiency and promote the effectiveness of photodynamic therapy (PDT) with the assistance of 633 nm laser irradiation. The unique nanoprobes show superior penetration and a retention time of more than six days with less accumulation in the liver allowing highly effective tumor recognition and monitoring. Additionally, there was little damage to healthy organs or tissues. These exciting new nanoprobes could be potential building blocks to develop new clinical therapies and translational medicine.
研究领域:
生物医学工程
·
癌症研究
·
分子影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Micromachines
·
2022
·Vol. 13
·期 7
·1057
·
IF 3.523
Yuanhui Wu,Meizhi Yang,Dan Long,Fanian Yang,Suxing Luo
Natural organic matters (NOMs) are ubiquitous in the environment, but few systematic studies have examined the influence of NOMs on the sorption ability of magnetic sludge biochar. In this study, magnetic sludge biochar was synthesized, characterized, and used as a sorbent to remove tetracycline (TC) from aqueous solutions. The effects of pH, humic acid (HA), and fulvic acid (FA) on TC adsorption by magnetic sludge biochar were studied using batch experiments. Adding HA and FA can alter the adsorption behavior of TC, except for its pH dependency. The results of this study show that relatively low concentrations of dissolved HA (≤8 ppm) and FA (≤5 ppm) promote the adsorption capacity of TC, but higher concentrations compete against TC for sorption sites on the surface of magnetic sludge biochar. The results of this study promote a better understanding of the application of magnetic sludge biochar in real antibiotic wastewater.Keywords:adsorption;fulvic acid;humic acid;magnetic sludge biochar;tetracycline
研究领域:
环境科学
·
污染控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Ling Xiong,Liying Ma,Meiqi Zhao,Haojian Zhang,Shaohua Chen
In present work, nanoscale zero-valent iron (nZVI) was successfully stabilized using fulvic acid (nZVI-FA). Shewanella oneidensis MR-1 (MR-1) was used to enhance the reactivity of the nZVI-FA. The results demonstrated that nZVI-FA combined with the MR-1 (nZVI-FA/MR-1) system could effectively solve the aggregation and surface passivation of nZVI, thereby significantly enhancing the reactivity of nZVI for Cr(VI) removal. The Cr(VI) removal efficiency of the nZVI-FA/MR-1 system (100 %) within 24 h was apparently higher than that of the ZVI-FA system (64.43 %). The enhancement of Cr(VI) removal is because fulvic acid (FA) FA can stabilize nZVI against aggregation; MR-1 could rapidly reduce the Fe(III) (hydr)oxides (FeHOs) passivation layers on the surface of nZVI-FA to biogenic Fe(II) [bio-Fe(II)], which in turn reduced Cr(VI). X-ray photoelectron spectroscopy (XPS) analysis revealed that the Fe(II)/Fe(III) ratio (1.20) and Cr(III)/Cr(VI) ratio (2.53) of the precipitates in the nZVI-FA/MR-1 system were much higher than that those in the nZVI-FA system (0.55 and 1.40, respectively). Three-dimensional excitation-emission matrix (3D-EEM) spectral analysis confirmed that tyrosine, tryptophan, and protein-like substances in extracellular polymeric substances (EPSs) from MR-1 are vital for the removal of Cr(VI). The mechanism analysis demonstrated that most Cr(VI) was reduced to Cr(III); Cr(III) existed as Cr 2 O 3 , FeCr 2 O 4 and Cr x Fe 1-x (OH) 3 precipitates, and some of the Cr(VI) was removed from the solution by adsorption and complexation. This study offers a low-cost, efficient, and environmentally friendly strategy to enhance the reactivity of nZVI for Cr(VI) removal, which has broad prospective applications.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）