Regenerative Biomaterials
·
2024
·
IF 5.6
Hu Zhaofan,Zhang Yunyang,Zhang Jingjing,Zheng Ran,Yang Yang,Kong Fei,Li Haoran,Yang Xinyan,Yang Shuhui,Kong Xiangdong,Zhao Ruibo
Biomineralization-based cell-material living composites ex vivo showed great potential for living materials construction and cell regulation. However, cells in scaffolds with unconnected pores usually induce confined nutrient transfer and cell–cell communications, affecting the transformation of osteoblasts into osteocytes and the mineralization process. Herein, the osteoblast-materials living hybrids were constructed with porous PLLA microspheres using a rational design, in which cell-based living materials presented an improved osteoblast differentiation and mineralization model using rationally designed cell-microsphere composites. The results indicated that the microfluidic-based technique provided an efficient and highly controllable approach for producing on-demand PLLA microspheres with tiny pores (<5 μm), medium pores (5–15 μm) and large pores (>15 μm), as well as further drug delivery. Furthermore, the simvastatin (SIM)-loaded porous PLLA microsphere with ε-polylysine (ε-PL) modification was used for osteoblast (MC3T3-E1) implantation, achieving the cell-material living microhybrids, and the results demonstrated the ε-PL surface modification and SIM could improve osteoblast behavior regulation, including cell adhesion, proliferation, as well as the antibacterial effects. Both in vitro and in vivo results significantly demonstrated further cell proliferation, differentiation and cascade mineralization regulation. Then, the quantitative polymerase chain reaction or histological staining of typical markers, including collagen type I, alkaline phosphatase, runt-related transcription factor 2 and bone morphogenetic protein 2, as well as the calcium mineral deposition staining in situ, reconfirmed the transformation of osteoblasts into osteocytes. These achievements revealed a promising boost in osteogenesis toward mineralization at the microtissue level by cell-microsphere integration, suggesting an alternative strategy for materials-based ex vivo tissue construction and cell regulation, further demonstrating excellent application prospects in the field of biomineralization-based tissue regeneration.
研究领域:
组织工程
·
再生医学
·
生物材料学
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产品关联
DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2025
·
IF 6.3
Yuting Wang,Haiyan Zhang,Zhiguang Xu,Xuchu Yin,Qingpeng Wang,Tao Zhang,Yan Zhao
Passive radiative cooling has attracted extensive attention owing to its great potential in reducing energy consumption. Endowing common fabrics with radiative cooling ability is an effective personal cooling strategy to manage skin microenvironment and improve thermal comfort. Herein, we report the use of silk fibroin microspheres to impart cotton fabrics with radiative cooling ability, taking advantages of both the high infrared emittance of silk fibroin and the high solar reflectance of microspherical structure. The silk fibroin microspheres were prepared from an aqueous solution of regenerated silk fibroin through a simple ethanol-induced self-assembly approach. The diameter of microspheres (0.37–1.52 μm) and thus, the solar reflectance, can be easily adjusted by varying the ethanol amount. The general dip-coating method was used to apply the silk fibroin microspheres onto cotton fabrics. Remarkably, compared to pristine cotton fabric and pure silk fabric, the cotton fabric coated with silk fibroin microspheres achieved an average temperature drop of 5.1 °C and 2.3 °C, respectively, during daytime outdoor test. Moreover, the washing resistance of the silk fibroin microsphere coating can be greatly improved by applying an additional thin layer of polyacrylate adhesive, thereby demonstrating the practical application feasibility of coated cotton fabric for personal thermal management.
研究领域:
材料科学
·
纺织工程
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产品关联
DOI
高影响因子
Materials Today Bio
·
2024
·
IF 8.7
Kaixuan Ma,Lei Yang,Wenzhao Li,Kai Chen,Luoran Shang,Yushu Bai,Yuanjin Zhao
Hydrogel microspheres hold great promise as scaffolds for bone repair. Their hydrated matrix, biocompatibility, and functional properties make them an attractive choice in regenerative medicine. However, the irregularity of defect requires shape adaptability of the microspheres. Additionally, there is still room for improvement regarding the component of the microspheres to achieve sufficient bioactivity. Here, we prepare multi-bioactive microspheres composed of methacrylated silk fibroin (SFMA) via microfluidic electrospray. Magnesium ascorbyl phosphate (MAP) is encapsulated within the microspheres, whose sustained release facilitates angiogenesis and osteogenic differentiation. The microspheres are further coated with a polydopamine (PDA) layer, allowing them to assemble in situ into a scaffold that conforms to the non-uniform contours of bone defects. The photothermal conversion capability of PDA also provides mild photothermal stimulation to further promote bone regeneration. Based on the synergistic effects, our in vivo experiments demonstrated that the microsphere scaffold effectively promotes bone defect healing. Thus, this multi-bioactive scaffold offers a versatile strategy for bone repair with promising clinical potential.
研究领域:
生物材料学
·
再生医学
·
骨科
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2024
·
IF 4.6
Juan Zhou,Yanling Zhao,Yan Ling,Peng Zhao,Hui Gao,Yang Yang,Jinghua Chen
By recruiting stem cells into scaffolds and differentiating them into osteoblasts, stem cells can be mobilized to directly repair bone defects, which avoids a series of disadvantages of exogenous stem cell implantation. In this study, a microsphere-composite hydrogel for the recruitment and osteogenic differentiation of stem cells was constructed. Methacrylic anhydride modified gelatin (GelMA) and heparin (HepMA), as well as nanohydroxyapatite (nHAP), were used to prepare microspheres followed by adsorbing platelet-derived growth factor BB (PDGF-BB) whose loading efficiency was 53.7 ± 2.2%. Then the microspheres were compounded to the GelMA hydrogel encapsulated with simvastatin (SIM) to obtain microsphere-composite hydrogel GHnH-P@GS. GHnH-P@GS hydrogel could slowly release SIM and PDGF-BB, and the extents of release within 7 days were 44.1 ± 2.0% and 32.8 ± 1.1%. The synergistic effect of small molecule drugs and growth factors not only induced the recruitment of rabbit bone marrow-derived mesenchymal stem cells, but also promoted the osteogenic differentiation of stem cells, which was confirmed by experiments of cell migration, alkaline phosphatase, and alizarin red staining. Collectively, the microsphere-composite hydrogel GHnH-P@GS has a certain reference significance for the design of scaffolds for alveolar bone repair and regeneration.
研究领域:
组织工程
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再生医学
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生物医学材料
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干细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Huixuan Chen,Jiayu Li,Siwen Tao,Xinhua Tian,Xikun Sun,Ruiqin Gao,Ni Bai,Guo-Dong Li
Developing fast, accurate and sensitive triethylamine gas sensors with low detection limits is paramount to ensure the safety of workers and the public. However, sensors based on single metal oxide materials still suffer from drawbacks such as low response sensitivity and long response and recovery times. To address these challenges, in this work, a series of mesoporous CdO/CdGa 2 O 4 microspheres were synthesized. We optimized the sensor's sensing performance to triethylamine by fine-tuning the ratio of CdO to CdGa 2 O 4 . Among them, CdO:3CdGa 2 O 4 -based sensor demonstrates a rapid response time of 2 s to detect 100 ppm of triethylamine, with a high response value of 211 and exceptional selectivity. Furthermore, it exhibits a low detection limit of 20 ppb for triethylamine, making it suitable for practically testing fish freshness. Crucially, electron transfer between the heterojunctions increases the chemically adsorbed oxygen on the materials’ surface, thereby enhancing the sensor's response sensitivity to triethylamine. This discovery provides new insights and methodologies for the design of highly efficient triethylamine gas sensors.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
Composites Part C: Open Access
·
2020
·
IF 0
Xubing Fu,Xingke Zhao,Lanjie Li,Chenxu Zhou,Xia Dong,Dujin Wang,Guisheng Yang
The development of innovative methods to prepare graphene-based polymer composites with remarkable properties has become a priority due to the many practical applications of these microstructures. Herein, a new technology was used to fabricate composites by combining graphene materials and polymer microspheres. Polyamide 6 (PA6) microspheres were synthesised by reaction-induced phase separation method, and they were wrapped with few-layer graphene (FLG) or graphene oxide (GO) by exfoliated adsorption method, which allows FLG and GO sheets to form a three-dimensional network structure in the PA6 matrix. Electrical and mechanical characterisation led to a distinct improvement in the properties of PA6 composites, which were superior to traditional graphene/polymer composites. Moreover, the process proposed in this work proved to be an effective method to prepare graphene/polymer composites, suitable for industrial production, and can be extended to other kinds of composites with nanofillers.
研究领域:
材料科学
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纳米技术
✓ 已验证
DOI
Surface Topography-Metrology and Properties
·
2024
·Vol. 12
·期 2
·025016
·
IF 2
Shengli Chen,Jiyuan Zhu
Rapid corrosion rate is limiting the engineering application of magnesium alloys. In this paper, an attempt was made to produce a corrosion resistant coating on the surface of AZ91D magnesium alloy using hydrothermal method. The coating surface is covered with microspheres and has a dense structure, which effectively isolates the contact between the outside world and the substrate. The corrosion potential(E) raised from −1.57 V to −0.45 V compared to the bare sample. The corrosion current density and corrosion rate of the coating can be reduced by 6 orders of magnitude, and the polarization resistance raised by 6 orders of magnitude. The thickness of the coating is 45.4 μm at the moment, with a minimum of defects on the surface, resulting in excellent corrosion resistance in the immersion test. The coating also has good adhesion strength at 4B, which can expand the application range of magnesium alloy in corrosive environments.
研究领域:
材料科学
·
腐蚀与防护
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DOI
高影响因子
MACROMOLECULAR RESEARCH
·
2020
·Vol. 28
·期 6
·644-649
·
IF 2.047
Zhen Shuang,Zhao Cheng,Zhao Xin,Ji Wu,Li Jieshou
Gut microbiota play an important role in many diseases. However, few researches evaluated the effect of faecal bacteria transplantation in protecting gut barrier during colitis. In this study, we focused on the effect of faecal microbiota encapsulated alginate microsphere on dextran sulfate sodium (DSS) induced colitis mice. Bacteria are sensitive to gastric juice and digestive enzymes, which is the dilemma of utilizing microbiota. Through taking advantage of alginate, microspheres that could protect its contents were fabricated. In vitro drug release experiment demonstrated their ability in protecting contents in simulated gastric fluid and release contents in simulated intestinal fluid. Thus, the resultant microsphere could be used in faecal microbiota transplantation. In vivo , the transplantation of faecal microbiota from healthy mice showed protection against DSS induced oxidative stress and gut barrier damage. Our findings suggest that the faecal microbiota transplantation could be a potential effective treatment for protecting gut barrier in inflammatory bowel diseases (IBD) colitis with faecal microbiota encapsulated alginate microsphere.
研究领域:
胃肠病学
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微生物学
·
药剂学
✓ 已验证
DOI
高影响因子
Soft Matter
·
2026
·
IF 2.9
Jie Ren,Wenjing Zhang,Yu Wang,Ziqiong Zhou,Yan Li,Yang Wu
Hydrogel-based flexible sensors are attractive for wearable electronics because of their softness, conformability, and tissue-like mechanics. Nevertheless, integrating high toughness, robust adhesion, self-healing ability, anti-freezing capability, and reliable strain sensitivity into a single hydrogel remains challenging. Here, we report a multifunctional conductive hydrogel in which ionic liquid-functionalized microspheres (MS) are introduced into an oxidized xanthan gum/chitosan (OXG/CS) matrix to serve as reinforcing nodes, dynamic physical crosslinking sites, and sacrificial energydissipation units. The microspheres interact strongly with the surrounding polymer chains through hydrogen bonding and electrostatic interactions, while the OXG/CS network provides reversible imine bonds. This hybrid dynamic network markedly improves the mechanical performance of the hydrogel, yielding an elongation at break of more than 970%, a toughness of 320 kJ m⁻³, and strong interfacial adhesion, while retaining excellent self-healing behavior. The incorporation of ionic species also endows the hydrogel with high conductivity (4.1 S m⁻¹) and excellent anti-freezing performance. As a strain sensor, the hydrogel exhibits high sensitivity, with a gauge factor of 6.70 in the 100-600% strain range, and can reliably detect subtle physiological signals such as pulse waves and vocal-cord vibrations. These results demonstrate that ionic microsphere reinforcement is an effective strategy for constructing multifunctional hydrogel sensors for use in complex environments.
✓ 已验证
DOI
PubMed
高影响因子
ACS Omega
·
2021
·
IF 3.512
Yan Zeng,Xiaokai Li,Xia Liu,Yuzhou Yang,Zhimin Zhou,Jincai Fan,Haiyue Jiang
Microtia, frequently encountered in plastic surgery practice, is usually corrected by auricular reconstruction with prostheses or autologous cartilages. In recent decades, however, cartilage tissue engineering has been emerging as a promising alternative for its minimal invasion and low immunogenicity. As a critical factor for tissue engineering, scaffolds are expected to be sufficiently porous and stiff to facilitate chondrogenesis. In this work, we introduce novel poly-l-lactic acid (PLLA) porous microsphere-reinforced silk-based hybrid (SBH) scaffolds with a multihierarchical porous structure. The scaffolds are fabricated by embedding PLLA porous microspheres (PMs) into a blending matrix of silk fibroin (SF) and gelatin solution, followed by mixing with a degummed silk fiber mesh and freeze-drying process. Through adjusting the amount of PLLA PMs, the mechanical strength approximates to natural cartilage and also balanced physical properties were realized. Biological evaluations of SBH scaffolds, both in vitro and in vivo, were conducted and PM-free plain silk-based (PSB) scaffolds were applied as control. Overall, it suggests that the incorporation of PLLA PMs remarkably improves mechanical properties and the capability to promote chondrogenesis of SBH scaffolds, and that SBH scaffolds appear to be a promising construct for potential applications in auricular cartilage tissue engineering and relevant fields.
研究领域:
生物医学工程
·
组织工程
·
整形外科
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Dongying Zhang,Qianqian Ouyang,Zhang Hu,Sitong Lu,Weiyan Quan,Puwang Li,Yu Chen,Sidong Li
Chitosan-based thermosensitive hydrogels have been widely used in drug delivery and tissue engineering, but their poor bioactivity has limited their further applications. Integral active oyster peptide microspheres (OPM) with an average particle diameter of 3.9 μm were prepared with high encapsulation efficiency (72.8%) and loading capacity (11.9%), exhibiting desirable sustained release effects. Using catechol functionalized chitosan (CS-C) as the polymeric matrix, OPM as the filler, and β-sodium glycerophosphate (β-GP) as a thermal sensitizer, the thermosensitive hydrogel CS-C/OPM/β-GP was prepared. Besides, the application of the hydrogel on wound healing was studied, and its biosafety was evaluated. The results of cell migration in vitro showed that the cell migration rate of CS-C/OPM/β-GP reached 97.47 ± 5.41% within 48 h, indicating that the hydrogel accelerated the migration of L929 cells. As demonstrated in the mouse skin wound experiment, CS-C/OPM/β-GP hydrogel not only inhibited the aggregation of diversified inflammatory cells and accelerated the generation of collagen fibers and new blood vessels of the wound, but also enhanced the synthesis of total protein (TP) in granulation tissue, and up-regulated the expression of Ki-67 and VEGF in the injury, thereby achieving fast wound healing. Safety evaluation results showed that CS-C/OPM/β-GP hydrogel was not cytotoxic to L929 cells, and the hemolysis ratio was less than 5% within 1 mg/mL. In conclusion, CS-C/OPM/β-GP hydrogel is expected as a promising medical dressing for wound healing.
研究领域:
材料科学
·
生物医学工程
·
组织工程
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Shuguang Wang,Kun Shi,Jiawei Lu,Weilian Sun,Qinghui Han,Lingbin Che,Dong Zhang
As a common complication of spine surgery, postoperative epidural fibrosis is an important cause of failed back surgery syndrome (FBSS), yet there is no effective clinical intervention to tackle it. Herein, for the first time, we develop a strategy of combining a gelatin methacryloyl (GelMA) hydrogel matrix with poly(lactic-co-glycolic acid) (PLGA) microsphere-encapsulated resveratrol (RSV), which aims to synergistically promote the inhibition effect on epidural fibrosis. The resultant RSV@PLGA-GelMA (8% w/v) hydrogels possess optimal mechanical properties and prompt the matrix sustainably and stably to release RSV for several weeks. It is further shown that the hybrid hydrogels without the drug exhibit good biosafety without distinct cytotoxicity, while RSV@PLGA-GelMA could prevent fibroblast proliferation and migration. Further rat laminectomy model indicates that the RSV@PLGA-GelMA hydrogels reduce epidural fibrosis by inhibiting fibroblast proliferation and extracellular matrix overexpression and deposition via a TGF-β/Smad signaling pathway. Consequently, we believe that such a creative structural combination will be a promising strategy for preventing postoperative epidural fibrosis of spine surgery.
研究领域:
生物医学工程
·
药学
·
神经外科
✓ 已验证
产品关联
DOI
PubMed
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