JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2025
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IF 3.1
Jizhou Li,Shiruoyu Zhou,Ruining Bai,Zhifeng Fu
Timely and sensitive monitoring of pathogens in clinical specimen is highly demanded for efficient control and precise treatment of infectious diseases. Herein, a 3D-printed maneuverable device integrating incubation, washing, and detection functions was manufactured for colorimetry of Pseudomonas aeruginosa ( P. aeruginosa ) using transparent resin. The device combined with magnetic beads (MBs) can achieve specific separation and efficient enrichment of P. aeruginosa . Furthermore, it can be directly fixed onto a 96-well plate holder for colorimetry. Specifically, a P. aeruginosa bacteriophage termed as JZ1 acquired from river water was applied as a recognition reagent to functionalize the separation vectors MBs. Then, a nanoconfinement MOFs material termed as PCN-222(Pt) with remarkable peroxidase-like activity was conjugated with polymyxin B to act as a signal tracer. With the formation of target bacterial complexes, the bound PCN-222(Pt) catalyzed the color reaction of 3,3′,5,5′-tetramethylbenzidine to enable quantitative colorimetry of P. aeruginosa by the maneuverable device. With this device, P. aeruginosa can be quantified within 40 min, with a dynamic range of 1.9 × 10 2 ∼ 1.9 × 10 6 cfu mL −1 . The results for colorimetry of P. aeruginosa in diverse sample matrixes demonstrated its satisfactory practicability. This work provides a facile, timely, and cost-effective technique for point-of-care testing of pathogens.
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RESPIRATORY RESEARCH
·
2023
·Vol. 24
·期 1
·1-19
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IF 5.8
Liu Lin,Fan Haiyang,Li Liang,Fan Yunping
Background Type 2 diabetes mellitus (T2DM) is widely prevalent worldwide, and respiratory tract infections (RTIs) have become the primary cause of death for T2DM patients who develop concurrent infections. Among these, Pseudomonas aeruginosa infection has been found to exhibit a high mortality rate and poor prognosis and is frequently observed in bacterial infections that are concurrent with COVID-19. Studies have suggested that acarbose can be used to treat T2DM and reduce inflammation. Our objective was to explore the effect of acarbose on P. aeruginosa RTI in T2DM individuals and elucidate its underlying mechanism. Methods High-fat diet (HFD) induction and P. aeruginosa inhalation were used to establish a RTI model in T2DM mice. The effect and mechanism of acarbose administered by gavage on P. aeruginosa RTI were investigated in T2DM and nondiabetic mice using survival curves, pathological examination, and transcriptomics. Results We found that P. aeruginosa RTI was more severe in T2DM mice than in nondiabetic individuals, which could be attributed to the activation of the NF-κB and TREM-1 signaling pathways. When acarbose alleviated P. aeruginosa RTI in T2DM mice, both HIF-1α and NF-κB signaling pathways were inhibited. Furthermore, inhibition of the calcium ion signaling pathway and NF-κB signaling pathway contributed to the attenuation of P. aeruginosa RTI by acarbose in nondiabetic mice. Conclusions This study confirmed the attenuating effect of acarbose on P. aeruginosa RTIs in T2DM and nondiabetic mice and investigated its mechanism, providing novel support for its clinical application in related diseases.
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Food Bioscience
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2025
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IF 5.9
Hongwei Zhan,Xiang Zou,Jiao Li,Lixiu Zhang,Guanyuan Qu,Yanqing Li,Yongjun Sun,Wenming Ju,Mengqi Ye,Yang Deng,Ting Ding
Pseudomonas fluorescens is a prevalent spoilage bacterium in various chilled aquatic products, producing toxic biogenic amines (BAs) through the decarboxylation of amino acids via amino acid decarboxylases. Our previous studies revealed that P. fluorescens PF12 regulates BA production through its quorum sensing (QS) system, suggesting that disrupting QS could be a potential approach to control BA generation. In this study, we targeted the three-dimensional structure of the LuxI-type protein in P. fluorescens PF12 to screen for QS inhibitors (QSIs) through high-throughput screening from Seaweed Metabolite Database and Comprehensive Marine Natural Products Database. Nine compounds with high binding scores to the LuxI-type protein were identified. Considering cost-effectiveness and safety, phytol was selected for further investigation. Experimental results demonstrated that sub-inhibitory concentrations of phytol significantly suppressed BA production, siderophore production, motility, extracellular protease secretion, and biofilm formation in P. fluorescens PF12. Specifically, when the salmon fillets were stored for 288 h, the histamine content in the fillets treated with 25 mg/mL phytol decreased by 43.81 %. RT-qPCR revealed that the expression levels of key genes hisD and hisC related to histamine synthesis in the phytol treatment group were reduced by 7.68 ( p < 0.05) and 8.23 % ( p < 0.05), respectively, compared to the control group. Molecular docking analysis revealed that phytol interacts with key residues in the LuxI-type protein, thereby blocking the synthesis of acyl-homoserine lactones (AHLs) and downstream QS signaling. These findings indicate that phytol has great potential for controlling BA production in aquatic products and ensuring food safety.
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Journal of Cleaner Production
·
2024
·
IF 9.7
Chun-dan Gan,Jin-yan Yang,Jiang-fei Li,Meng-qi Yang,Xin-yue Du,Aleksander Nikitin
The continuously elevated concentration of vanadium (V) in the environment is of great concern due to its potential risks to organisms. Microbial reduction is considered a green way to remediate the V-contaminated sites. This study presents the first demonstration of Pseudomonas balearica , a bacterium flourishing in vanadium-titanium magnetite tailings, exhibiting the capacity to reduce pentavalent V [V(V)]. We found that P. balearica exhibited strong tolerance to high V(V) levels, despite lacking known V(V)-reducing genes. After 48 h of incubation, the reduction rates of V(V) by P. balearica under 0.5 mM and 10 mM V(V) stress reached 81.1% and 60.6%, respectively. The phosphorus group and carboxyl group on the bacterial cell surface contributed to V(V) binding and reduction. Both extracellular and intracellular V(V) reduction was significantly facilitated by many critical V-induced differentially expressed genes (DEGs) encoding nitrate/nitrite reductases, ABC transporters , NADH, and c-type cytochromes . In addition, up-regulation of DEGs associated with reactive oxygen species (ROS)-scavenging enzymes , such as glutathione peroxidase, superoxide dismutase , and catalase , was observed as a protective response to oxidative stress triggered by V(V). Moreover, some up-regulated DEGs ( ureC , ureE , ureA , and ureG ) encoding urease subunit or urease accessory proteins significantly enriched in the nickel cation binding pathway, indicating that P. balearica may be resistant to Ni stress by regulating urease activity. This study will help to more thoroughly elucidate the characteristics and mechanisms of V(V) reduction by microbes and provide a native bacterium flourishing in tailings for potential bioremediation of V pollution and bioleaching of V-sources.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-19
·
IF 12.6
Du Chunhong,Wang Shuai,Cheng Yijie,Li Jie,Zhang Yufei,Li Zhuohao,Zhu Baolin,Wu Zhongming,Zhang Xinge,Zhou Lingyi
Pneumonia caused by Pseudomonas aeruginosa ( P. aeruginosa ) infection remains a formidable clinical challenge due to persistent biofilm formation and intrinsic antibiotic resistance, exacerbated by bacterial iron homeostasis that stabilizes biofilm architecture and neutralizes oxidative stress. Herein, we present Fe/TNT@NM, a biomimetic nanosonosensitizer activated by ultrasound (US) to dismantle biofilms through dual extracellular-intracellular mechanisms. The nanosonosensitizer features an iron-doped titanate nanotube (Fe/TNT) core encapsulated within a neutrophil membrane (NM). Under US irradiation, Fe/TNT@NM generates sonodynamic reactive oxygen species (ROS) extracellularly and enhances Fe 3+ release. These ions catalyze the Fenton reaction extracellularly to amplify chemodynamic effects and disrupt intracellular iron homeostasis, triggering bacterial ferroptosis. The NM coating enables immune evasion and biofilm-targeted delivery. This ultrasound-reinforced ferroptosis strategy synchronizes extracellular ROS storms with intracellular iron dyshomeostasis, achieving dual-action biofilm dismantling and eradication of drug-resistant P. aeruginosa . In a murine pneumonia model, Fe/TNT@NM suppresses biofilms and mitigates pulmonary injury. By converging biomimetic targeting, sonodynamic-chemodynamic cascades, and ultrasound-augmented ferroptosis, this nanosonosensitizer presents a paradigm-shifting approach to combat refractory biofilm infections and antibiotic resistance. Graphical
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BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Jiamu Li,Aijia Sun,Han Lai,Chuanhe Li,Huayi Li,Zhengchun Yang,Peng Pan,Jie He,Rui Zhang,Chunhong Wang
In this study, an electrochemical sensor for the specific detection of Pseudomonas aeruginosa ( P. aeruginosa ) was developed using an F23 aptamer-functionalized nitrogen-doped multi-walled carbon nanotubes (N doped-MWCNTs) and silver nanoparticles (AgNPs) composite. Systematic optimization of the Ag/C ratio revealed that a 1:10 composition delivers superior electrochemical performance, owing to synergistic effects between highly dispersed AgNPs and efficient nitrogen doping. Then a biosensor was constructed based on a three-electrode system, featuring a screen-printed electrode (SPE) modified with optimized N-doped MWCNTs/AgNPs-10/F23 aptamer as the working electrode. The structural and compositional characteristics of the sensor materials were systematically characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). The electrochemical performance was evaluated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) to assess the conductivity and charge transfer properties of the electrode materials. The sensor exhibited a wide linear detection range from 10 −1 to 10 6 CFU·mL −1 and the limit of detection is 0.0798 CFU·mL −1 , demonstrating high specificity and sensitivity for P. aeruginosa . This study demonstrates a novel strategy for developing cost-effective, portable biosensors with exceptional selectivity for bacterial pathogen detection, offering significant potential for real-time environmental monitoring and point-of-care diagnostic applications.
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ACS Applied Materials & Interfaces
·
2025
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IF 8.2
Rui Chen,Jinjie Wang,Yuhang Wang,Xin Liu,Zhiqi Yang,Yiping Ren,Yunan Yang,Tianshu Zou,Yan Yan,Liqi Wei,Biao Zhang,Lili Wang,Yining Chen,Hongxia Ma,Yan Cheng
The emergence of multidrug-resistant bacteria and persistent inflammatory responses have exacerbated the therapeutic difficulty of bacteria-infected pneumonia. There is an urgent need to find new strategies for synchronous sterilization and inflammation regulation for pneumonia. Herein, we developed maleimide-modified and alantolactone (Ala)-loaded copper indium selenide nanoparticles (ACIS NPs) to achieve near-infrared II (NIR-II) light-triggered photodynamic immunotherapy for multidrug-resistant Pseudomonas aeruginosa-infected pneumonia. After intranasal administration, they can quickly penetrate the mucus layer and then target P. aeruginosa and attach to its biofilm, achieving bacterial inactivation and biofilm elimination under NIR-II light irradiation. Simultaneously, ACIS NPs can also release Ala at the lesion site. They can not only stimulate the maturation of phagolysosomes in macrophages to increase phagocytosis efficiency but also downregulate the expression of NF-κB to reduce inflammatory cytokines and relieve the inflammatory level, thus promoting the polarization of macrophages toward an anti-inflammatory phenotype. Both in vitro and in vivo experiments have demonstrated that ACIS NPs can accumulate in lung tissues for killing P. aeruginosa and regulating macrophages, exhibiting high therapeutic efficacy against bacteria-infected pneumonia. This study demonstrates the potential of ACIS NPs as an alternative to traditional antibiotics, offers hope against bacterial resistance and the inhibition of biofilm formation, regulates the disordered inflammatory environment of the body, and provides a new strategy for bacteria-infected pneumonia therapy.
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VACCINE
·
2026
·
IF 3.4
Xiaotong Wang,Chanyuan Dai,Xiang Ji,Haiyue Zheng,Hao Wu,You Yang
Pseudomonas aeruginosa is a critical-priority pathogen responsible for life-threatening infections in immunocompromised individuals, yet its extensive antigenic heterogeneity has impeded the development of immunotherapeutics. Alginate, a structurally conserved surface polysaccharide that forms the backbone of the biofilm matrix of mucoid P. aeruginosa strains in chronic infections, represents a compelling target. Here, we report the generation and functional evaluation of mAb 546B, a murine IgG1 monoclonal antibody directed against the synthetic mannuronic acid tetrasaccharide epitope 1 . This antibody bound to the surface alginate of both mucoid and nonmucoid P. aeruginosa strains and induced pronounced bacterial aggregation. Notably, 546B inhibited biofilm formation, disrupted established biofilms, and reduced the minimum inhibitory concentration of meropenem across multiple strains. In a bacterial clearance model, 546B monotherapy reduced lung bacterial burden compared to PBS controls, and its combination with meropenem achieved a marked reduction accompanied by significantly attenuated pulmonary inflammation and histopathological injury. In a lethal PAO1 pneumonia model, 546B alone improved the 7-day survival rate of mice, and combination with meropenem could further enhance the survival rate. These findings establish 546B as a promising monoclonal antibody that targets alginate-mediated biofilms, supporting its further development as a passive immunotherapeutic against P. aeruginosa infection.
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Synthetic and Systems Biotechnology
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2023
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IF 4.8
Siyang He,Weiwei Wang,Weidong Wang,Haiyang Hu,Ping Xu,Hongzhi Tang
Industrial expansion has led to environmental pollution by xenobiotic compounds like polycyclic aromatic hydrocarbons and monoaromatic hydrocarbons. Pseudomonas spp. have broad metabolic potential for degrading aromatic compounds. The objective of this study was to develop a “biological funneling” strategy based on genetic modification to convert complex aromatic compounds into cis,cis -muconate (ccMA) using Pseudomonas putida B6-2 and P. brassicacearum MPDS as biocatalysts. The engineered strains B6-2 (B6-2ΔcatBΔsalC) and MPDS (MPDSΔsalC(pUCP18k-catA)) thrived with biphenyl or naphthalene as the sole carbon source and produced ccMA, attaining molar conversions of 95.3% (ccMA/biphenyl) and 100% (ccMA/naphthalene). Under mixed substrates, B6-2ΔcatBΔsalC grew on biphenyl as a carbon source and transformed ccMA from non-growth substrates benzoate or salicylate to obtain higher product concentration. Inserting exogenous clusters like bedDC 1 C 2 AB and xylCMAB allowed B6-2 recombinant strains to convert benzene and toluene to ccMA. In mixed substrates, constructed consortia of engineered strains B6-2 and MPDS specialized in catabolism of biphenyl and naphthalene; the highest molar conversion rate of ccMA from mixed substrates was 85.2% when B6-2ΔcatBΔsalC was added after 24 h of MPDSΔsalC(pUCP18k-catA) incubation with biphenyl and naphthalene. This study provides worthwhile insights into efficient production of ccMA from aromatic hydrocarbons by reusing complex pollutants.
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BIODEGRADATION
·
2022
·Vol. 34
·期 1
·73-81
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IF 3.731
Gu Chen,Liang Jinxuan,Liu Ming,Rui Jianliang,Shi Jiyan,Yu Yanming,Zhang Xuwang
Bisphenol A (BPA) has been widely used in the manufacture of polymeric materials. BPA is regarded as an endocrine disrupting chemical, posing a great threat to the public health. In this study, a bacterial strain LM-1, capable of utilizing BPA as the sole carbon and energy source under aerobic conditions, was originally isolated from an activated sludge sample. The isolate was identified as Pseudomonas sp. based on 16S rRNA gene sequence analysis. Strain LM-1 was able to completely degrade 25–100 mg/L BPA within 14–24 h, and it also exhibited high capacity for BPA degradation at a range of pH (6.0–8.0). (NH 4 ) 2 SO 4 and NH 4 NO 3 were the suitable nitrogen sources for its growth and BPA biodegradation, and the BPA degradation could be accelerated when exogenous carbon sources were introduced as the co-substrates. Metal ions such as Zn 2+ , Cu 2+ , and Ni 2+ could considerably suppress the growth of strain LM-1 and BPA degradation. According to the analysis of liquid chromatography coupled to Q-Exactive high resolution mass spectrometry, hydroquinone, p -hydroxybenzaldehyde, and p -hydroxybenzoate were the predominate metabolites in the BPA biodegradation and the degradation pathways were proposed. This study is important for assessment of the fate of BPA in engineered and natural systems and possibly for designing bioremediation strategies.
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CHEMOSPHERE
·
2022
·
IF 8.943
Juping You,Jie Shao,Jianmeng Chen,Dongzhi Chen
A new strain, Pseudomonas sp. SJ-1, which was able to remove model odorous organics methanethiol (MT) has been isolated from the wastewater treatment plant and identified via 16S rRNA analysis . Initial MT concentration, temperature and pH played an important role in MT removal, and up to 100% of 260 mg L −1 of MT could be removed within 11 h under the optimum conditions (30 °C, pH 7.0) with an average degradation rate of 23.6 mg L −1 h −1 , which was the highest one in literature so far. The silicone particles were added as the non-aqueous phases (NAP) to enhance the performance of MT degradation. The results indicated that the maximum degradation rate and specific cell growth of strain SJ-1 were 2.36 times and 1.31 times by Haldane kinetic model analysis in the NAP added test. The SO 4 2− was identified as the major intermediate and CO 2 as a final product in MT biodegradation. Overall, this is the first report that a newly isolated Pseudomonas sp. could use high concentration MT as sole energy source and carbon source and its activity could be enhanced by adding NAP. The results provide a suggestion for the development of more effective and reliable biological treatment processes.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
·
IF 7.129
Xiaohui Liu,Jing Chen,Ying Liu,Zhengfen Wan,Xiaochun Guo,Shaoyong Lu,Dongru Qiu
The antibiotic-degrading ability and mechanism of the bacteria in the novel and ecological bioelectrochemical technology-integrated constructed wetlands (BICW) remain unknown. In this study, the sulfamethoxazole (SMX) degrading strain Pseudomonas silesiensis F6a (F6a), which had high degradation efficiency, was firstly isolated from a substrate sample in BICW. The SMX degradation process of F6a follows pseudo first order kinetics. Four metabolic pathways and twelve degradation products were identified. Based on genomics and proteomics analysis, six key SMX-degrading genes, Gene4641 deoC , Gene0552 narI , Gene0546 luxS , Gene1753 nuoH , Gene0655 and Gene4650, were identified, which were mainly participated in C-S cleavage, S-N hydrolysis and isoxazole ring cleavage. Interestingly, we found the corresponding sulfonamides resistance genes were not detected in F6a, which may provide an evidence for low abundance of the sulfonamides resistance genes in BICW system. These findings would contribute to a better understanding of biotransformation of antibiotic in the BICW.
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