RSC Advances
·
2020
·Vol. 10
·期 12
·6801-6806
·
IF 3.119
Pengyu Xiao,Yang Liu,Wenjing Zong,Jin Wang,Minghuo Wu,Jingjing Zhan,Xianliang Yi,Lifen Liu,Hao Zhou
Multiple enzyme-like activities of manganese oxides (MnO2) have been reported and applied in catalysis, biosensors, and cancer therapy. Here, we report that catechol can be determined colorimetrically based on the 3,3′,5,5′-tetramethylbenzidine (TMB) oxidase-like activity of δ-MnO2. The detection was based on pre-incubation of catechol containing water samples with δ-MnO2, and then the residual TMB oxidase-like activity of reacted δ-MnO2 was linearly dependent on the catechol concentration in the range of 0.5 to 10 μM. This determination method was stable at pH 3.73–6.00 and was not affected by ion strength up to 200 μM. Common co-solutes in water bodies (50 μM) have negligible effects and excellent selectivity of catechol among various phenolic compounds (15 μM) was facilitated. Both reduction and aggregation of δ-MnO2 were observed during the incubation process with catechol, and aggregation-induced TMB oxidase–mimic activity decrease was the main factor for this colorimetric determination.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2017
·Vol. 64
·期 7
·795-803
·
IF 0.935
Junyu Lu,Lianqiang Wei,Dongmei Yao,Xiuju Yin,Hongfang Lai,Xiuxiang Huang
β-AgVO 3 nanorods have been demonstrated to exhibit intrinsic peroxidase-like activity. The oxidation of glucose can be catalyzed by glucose oxidase (GO x ) to generate H 2 O 2 in the presence of O 2 . The β-AgVO 3 nanorods can catalytically oxidize peroxidase substrates including o -phenylenediamine (OPD), 3,3′,5,5′-tetramethylbenzidine (TMB), and diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) by H 2 O 2 to produce typical color reactions: OPD from colorless to orange, TMB from colorless to blue, and ABTS from colorless to green. The catalyzed reaction by the β-AgVO 3 nanorods was found to follow the characteristic Michaelis–Menten kinetics. Compared with horseradish peroxidase and AgVO 3 nanobelts, β-AgVO 3 nanorods showed a higher affinity for TMB with a lower Michaelis–Menten constant ( K m ) value (0.04118 mM) at the optimal condition. Taking advantage of their high catalytic activity, the as-synthesized β-AgVO 3 nanorods were utilized to develop a colorimetric sensor for the determination of glucose. The linear range for glucose was 1.25–60 μM with the lower detection limit of 0.5 μM. The simple and sensitive GO x -β–AgVO 3 nanorods–TMB sensing system shows great promise for applications in the pharmaceutical, clinical, and biosensor detection of glucose.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 8
·2481-2489
·
IF 4.831
Lu Yuan,Yu Jing,Ye Weichun,Yao Xin,Zhou Panpan,Zhang Haixia,Zhao Suqin,Jia Lipei
The authors describe a colorimetric method for the determination of Hg(II) ions by exploiting the peroxidase-lile activity of few-layered MoS 2 nanosheets (MoS2-NSs). These were prepared by sonication-induced exfoliation of bulk MoS 2 crystals in aqueous surfactant solution. The MoS2-NSs were found to acts as a peroxidase mimic that is capable of oxidizing the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2) to give a blue product with an absorption maximum at 652 nm. The addition of Hg(II) strongly accelerates the kinetics of this reaction. It is shown that the enzyme mimic possesses a high affinity for TMB and a lower pseudo-Michaelis-Menten constant. The stimulating effect of Hg(II) is seriously influenced by the change of surface charge. The use of nanosheets covered with (negatively charged) polystyrene sulfonate results in a decrease in the formation of blue dye, while those covered with (cationic) poly(diallyldimethyl ammonium) ions cause a small increase. Under optimal conditions, the peroxidase-like activity of MoS2-NSs is affected by Hg(II) in the 2.0 to 200 μM concentration range. The method has a detection limit (LOD) of 0.5 μM which is much below the allowed level in cosmetics (1 ppm; ca. 5 μM). The method display excellent sensitivity, selectivity and stability. It was applied to the determination of total mercury in cosmetic samples, and results compared well with results obtained by ICP-AES.
A spectrophotometric assay for mercury - (II) determination is reported that is based on Hg 2+ -stimulation effect on the 3,3′,5,5′-tetramethylbenzidine (TMB)-H 2 O 2 reaction system catalyzed by MoS 2 nanosheets.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 38
·18048-18053
·
IF 3.591
Rui Shi,Qiaoling He,Shiqi Cheng,Bolin Chen,Yilin Wang
MoS2 nanosheets (MoS2 NSs), which exhibited peroxidase-like activity, were prepared using the hydrothermal method. The microstructure and element speciation of the MoS2 NSs were characterized using transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS), respectively. The obtained MoS2 NSs could catalyze the H2O2-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form blue oxidized TMB (ox TMB) with an absorption peak at 652 nm. The effects of MoS2 NS concentration, incubation time, temperature, and acidity on the color development of TMB were investigated. Under the optimal conditions (1.0 μg mL−1, 40 min, 40 °C, pH 4.0), the increase in absorbance, which was induced by MoS2 NS-catalyzed TMB oxidation, enabled the detection of H2O2 ranging from 2.5 μmol L−1 to 300 μmol L−1. As H2O2 is one of the products of glucose oxidase (Gox)-catalyzed glucose oxidation, a colorimetric method was further established for measuring the concentration of glucose. The absorbance response was linearly dependent on glucose concentration of 2.5–200 μmol L−1, with a detection limit of 2.1 μmol L−1. The proposed method was applied to the assay of glucose in urine samples with satisfactory results.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 29
·13952-13962
·
IF 3.3
Dehong Bai,Ziyu Xue,Shaohui Li,Ran Meng,Dongxia Zhang,Xibin Zhou
Chromate (Cr(VI)) is a highly toxic heavy metal ion that not only endangers human health but also causes environmental pollution. To control and remove Cr(VI) from water more effectively, we report a simple, novel, and rapid detection technique for Cr(VI) in water based on an iron-manganese bimetal-metal organic framework (FexMny-MOF). Firstly, the synthesized FexMny-MOF has a large specific surface area and porosity; for the first time, an MOF with dual mimic enzyme properties is reported, which can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue product in the presence or absence of H2O2. Interestingly, the mimic enzyme properties of FexMny-MOF are enhanced than those of Fe-MOF and Mn-MOF. The free energies of elementary reactions and adsorption energies (Eads) of H2O2 and O2 adsorbed on different materials were calculated by density functional theory (DFT) to verify the mechanism of action of FexMny-MOF. The Fe1Mn1-MOF mimics oxidase-like oxidation of TMB; 8-hydroxyquinoline (8-HQ) is used as the inhibitor of TMB oxidation, and the specific interaction between 8-HQ and Cr(VI) weakens the inhibition of 8-HQ on TMB oxidation to achieve the detection of Cr(VI). The established method has high sensitivity and selectivity with a detection limit of 51 nM and a linear range of 80–3500 nM. This method has been successfully applied to the determination of Cr(VI) in tap water and the Yellow River, showing its great potential in environmental monitoring.
研究领域:
环境科学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Analysis and Testing
·
2019
·Vol. 3
·期 3
·228-237
·
IF 0
Niu Xiangheng,Ye Kun,Li Zhibo,Zhao Hongli,Wang Linjie,Pan Jianming,Song Hongwei,Lan Minbo
Developing reliable and facile approaches for alkaline phosphatase (ALP) sensing is important due to its role as a clinical biomarker for many diseases. In this study, we proposed a new and convenient colorimetric assay based on the pyrophosphate (PPi)-mediated oxidase-mimicking activity switching of nanosized MnFe 2 O 4 for the detection of ALP. The synthesized MnFe 2 O 4 exhibited high oxidase-like activity to catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to its blue product TMBox in the presence of dissolved O 2 , leading to a color reaction rapidly and remarkably; PPi could significantly inhibit the activity of the MnFe 2 O 4 nanozyme via the strong interaction between PPi and the Fe(III) species in MnFe 2 O 4 , resulting in the suppression of the TMB color reaction; when ALP was added, it hydrolyzed the PPi substrate to phosphate (Pi) that had no obvious effect on the MnFe 2 O 4 activity, and such that the TMB color reaction catalyzed by the nanozyme could be observed again. With the above principle, linear colorimetric determination of ALP in the scope of 0.6–55 U L −1 was achieved, giving the limit of detection down to 0.27 U L −1 . Besides, the developed assay could provide selective response toward ALP against other co-existing biological species. Furthermore, reliable detection of ALP in human serum samples was verified by our assay, revealing its great promise as an effective and facile tool for ALP monitoring in clinical practice.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
医学诊断
✓ 已验证
DOI
JOURNAL OF NANOPARTICLE RESEARCH
·
2016
·Vol. 18
·期 3
·1-10
·
IF 2.101
Guo Leilei,Huang Kaixun,Liu Hongmei
Selenium nanoparticles (SeNPs) are considered to be the new selenium supplement forms with high biological activity and low toxicity; however, the molecular mechanism by which SeNPs exert the biological function is unclear. Here, we reported that biocompatibility SeNPs possessed intrinsic oxidase-like activity. Using Na 2 SeO 3 as a precursor and glutathione as a reductant, biocompatibility SeNPs were synthesized by the wet chemical reduction method in the presence of bovine serum albumin (BSA). The results of structure characterization revealed that synthesized SeNPs were amorphous red elementary selenium with spherical morphology, and ranged in size from 25 to 70 nm size with a narrow distribution (41.4 ± 6.7 nm). The oxidase-like activity of the as-synthesized SeNPs was tested with 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate. The results indicated that SeNPs could catalyze the oxidization of TMB by dissolved oxygen. These SeNPs showed an optimum catalytic activity at pH 4 and 30 °C, and the oxidase-like activity was higher as the concentration of SeNPs increased and the size of SeNPs decreased. The Michaelis constant ( K
m ) values and maximal reaction velocity ( V
max ) of the SeNPs for TMB oxidation were 0.0083 mol/L and 3.042 μmol/L min, respectively.
研究领域:
纳米生物学
·
生物化学
·
医学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Xuehong Zhang,Wei Liu,Xuemei Li,Zhen Zhang,Duoliang Shan,Hong Xia,Shouting Zhang,Xiaoquan Lu
Hexavalent chromium ion (Cr6+) is one of the most toxic substances for plants, for animals, and is a confirmed human respiratory carcinogen. However, so far, there are few independent and efficient colorimetric methods for detection of Cr6+. Here, we introduce a convenient, label-free, catalysis-based, and efficient strategy for quantification of Cr6+ by using a colorimetric sensing probe 3,3′,5,5′-tetramethylbenzidine (TMB). In the presence of a trace amount of gold amalgam nanocomposites (Au@Hg) and Cr6+, TMB can be oxidized to oxTMB and the color changed to an intense blue that was observed by naked-eye and absorption spectroscopic method. In addition, the colorimetric method shows the high selectivity against 34 other interfering substances, and it can be performed at room temperature, in water, and requires only ∼5 min. Thus, the catalysis-based colorimetric assay for accurate and ultrahigh selective identification of Cr6+ will find widespread use in the world.
研究领域:
环境科学
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jialu Pan,Man Li,Feng Zhang,Xiaoran Wang,Yanqing Cong,Shi-Wen Lv
It is of great significance to exploit simple and efficient methods for detecting hazardous substances in the fields related to food safety and environmental protection. Herein, for the first time, a newly-designed hollow Co-N/C nanocage with oxidase-like activity was synthesized via a feasible method. Benefiting from the construction of hollow structure and the introduction of carbon layer , the specific surface area of enzyme mimic was improved, so exposing more active sites. Importantly, hollow Co-N/C nanocage displayed an excellent performance in interfacial charge transfer, which provided convenience for the occurrence of chromatic reaction between enzyme mimic and TMB . As predicted, hollow Co-N/C nanocage can effectively oxidize colorless TMB to blue oxTMB in the absence of H 2 O 2 , and the analyses about catalytic mechanism suggested that both the redox couples of Co 3+ /Co 2+ and O 2 •− were involved in the oxidation of TMB. Furthermore, a chromogenic sensing method for the detection of Na 2 SO 3 and Cr 6+ was exploited, and a broad linear range and a low LOD can be achieved. More interestingly, developed detection method still possessed good recovery even in real samples. In a word, current work will share novel insights into the design of enzyme mimics and the analyses on chromogenic mechanism.
研究领域:
材料科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2017
·Vol. 52
·期 18
·10738-10750
·
IF 2.599
Guo Leilei,Mao Long,Huang Kaixun,Liu Hongmei
In this paper, platinum (Pt)–selenium (Se) nanostructures and Pt nanoparticles were synthesized by a facile one-step chemical reduction route and their catalytic performance was evaluated as oxidase mimic. The results of structure characterization revealed that Pt – Se nanostructures consist of Pt and Se atoms (the Pt/Se atomic molar ratio is approximately 7:3), while Pt nanoparticles consist of pure element Pt. The oxidase-like activity of Pt – Se nanostructures and Pt nanoparticles was evaluated with 3,3′,5,5′-tetramethylbenzidine (TMB) as substrate. The results indicated that Pt – Se nanostructures had a lower Michaelis constant ( K
m ) and higher catalytic constant ( K
cat ) for TMB oxidation than that of Pt nanoparticles,which mean the binary Pt – Se hybrid nanostructures had stronger binding affinity with TMB and higher catalytic activity in comparison with monometallic Pt nanoparticles. The enhanced oxidase-like activity of Pt – Se nanostructures may be due to element Se doped in binary Pt – Se hybrid nanostructures, which can accelerate electron transport and provide excellent chemical stability against catalytic performance degradation during the TMB oxidation reaction. Mercury (II) ions (Hg 2+ ) could inhibit the oxidase mimetic activity of Pt – Se nanostructures and resulted in a color change of the reaction system. Based on this mechanism, a facile colorimetric assay for Hg 2+ was developed with a detection limit as low as 70 nm and a linear range of 0–2.5 μM.
研究领域:
纳米材料
·
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
ACS Applied Nano Materials
·
2020
·
IF 0
Chun-Xia Zhang,Yan-Cai Gao,Hong-Wei Li,Yuqing Wu
Guanosine monophosphate (GMP)-protected bimetallic nanoclusters of gold and platinum, Au–PtNCs-GMP, were prepared through an improved hydrothermal method under basic conditions, which show high catalytic activity for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) under ambient conditions. Further systematic optimization of synthesis provides the best catalyst by feeding the materials 0.8 mM HAuCl4, 0.2 mM H2PtCl6, 4.0 mM NaOH, and 3.0 mM GMP, which reacted at 120 °C for 30 min, which show an initial maximum velocity (Vmax) of 253.8 × 10–8 M s–1 for TMB as the substrate. Such a value is at the top among the oxidase mimetics reported, which is essentially attributed to the synergistic effect between two metals in the small nanocluster. Moreover, the achieved Au–PtNCs-GMP show a short reaction time of 60 s for the oxidation of substrates, which is much faster than the previously reported ones. The in-depth mechanistic studies by 1H and 31P NMR spectroscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy of the optimized Au–PtNCs-GMP reveal them to be of ultrasmall size with a unique ligand structure, particularly the synergistic effect between the two metals endow the product with a high catalytic performance. Therefore, the present study not only provides a novel bimetallic nanocluster as a good catalyst with highly efficient oxidase mimetics but also opens a way for the development of other oxidase mimetics.
研究领域:
纳米生物学
·
催化化学
·
材料科学
✓ 已验证
DOI
RSC Advances
·
2015
·Vol. 5
·期 23
·17458-17465
·
IF 3.84
Qun Wei Shu,Chun Mei Li,Peng Fei Gao,Ming Xuan Gao,Cheng Zhi Huang
CuS materials with peroxidase activity have been prepared but greatly limited by the large dosage and low peroxidase activity. In this paper, porous hollow CuS nanospheres with a variety of sizes were fabricated by one-pot method based on a facile template-assisted hydrothermal approach. The size of the porous hollow CuS nanospheres could be simply tuned by adjusting the molar ratios of reactants, the reaction temperature, and time. The as-prepared porous hollow CuS nanospheres were demonstrated to exhibit more prominent intrinsic peroxidase-like activity using TMB as a peroxidase substrate in the presence of hydrogen peroxide (H2O2). This is a significant comparison to previous reports, demonstrating that the new developed synthetic porous hollow CuS nanospheres can be a new kind of candidate for peroxidase mimics, and the nanospheres are promising for applications in biosensors, environmental monitoring, and so on.
研究领域:
材料科学
·
生物医学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）