Nanomaterials
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2024
·Vol. 14
·期 23
·1958
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IF 4.4
Yan Mo,Xiaoping Zhang,Ke Zou,Wen Xing,Xiayang Hou,Yu Zeng,Yugang Cai,Ruixiang Xu,Hongwen Zhang,Weiping Cai
Etomidate (ET), a medical anesthetic, is increasingly being incorporated into e-liquids for consumption and abuse as a new psychoactive substance (NPS), leading to significant social issues. In this work, large-area Au micro- and nano-structured ordered arrays were engineered as surface-enhanced Raman spectroscopy (SERS) substrates for fast detection and precise identification of ET and its metabolites. This ordered array, characterized by abundant electromagnetic enhancement hotspots and structural uniformity, imparts unique properties to the SERS substrate, including ultra-sensitivity, spectral signal reproducibility, and precise quantitative capabilities. Furthermore, it effectively mitigates interference from the complex matrix of e-liquids, facilitating the rapid detection of trace amounts of ET molecules. This SERS rapid detection technology can act as a preliminary screening method for gold-standard spectroscopic analysis, facilitating the on-site rapid screening of suspicious samples and thereby enabling efficient detection and precise verification.
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Journal of Biomedical Nanotechnology
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2009
·Vol. 5
·期 5
·505-510
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IF 0.989
Li Zhiyang,He Lei,Shi Zhiyang,Wang Hua,Li Song,Liu Hongna,Wang Zhifei,He Nongyue
This paper described that Fe3O4 nanoparticles were encapsulated with methyl methacrylate (MMA) using linolenic acid (LA) as a crosslinking agent, after which the resulting polymethyl methacrylate (PMMA) embed Fe3O4 nanoparticles (PMMA/Fe3O4) were coated with silica, forming SiO2/(PMMA/Fe3O4) core–shell structure particles. Then these magnetic nanoparticles (MNPs) were applied in the developed system of chemiluminescent magnetic enzyme-linked immunoassay. E. coli O157:H7 was sandwiched between rabbits anti-E. coli O157:H7 polyclonal antibody-coated magnetite nanoparticles (immunomagnetic nanoparticles or IMNPs) and mouse anti-E. coli O157:H7 monoclonal antibody (E. coli O157-McAb). Commercial alkaline phosphatase conjugated horse anti-mouse immunoglobulin (ALP-Ab) was used to bond with the monoclonal antibody, finally the chemiluminescent signals were detected by adding 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane (AMPPD) which was the substrate reagent of ALP. The specificity and sensitivity of this system for detecting E. coli O157:H7 were researched. The results indicated that this method was of good specificity when using E. coli Top 10F′ and Vibrio cholera as negative controls. The detection limit was 103 cells mL−1 when the antigen solution was 1 mL, and the procedure duration was about 3 h.
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PLoS One
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2025
·Vol. 20
·期 5
·e0324164
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IF 2.6
Bingbing Cao,Jiali Zhou,Bo Xia,Xiaoqing Li,Rui Wang,Yiqiao Xu,Chunqi Li
Choline has been proven to be effective in maintaining liver function. However, the effect of choline, in combination with other nutrients, on the improvement of non-alcoholic fatty liver disease (NAFLD) remains unclear. This study aimed to investigate the potential effect of the nutraceutical complex containing choline bitartrate, zinc citrate, and dl-α-Tocopheryl acetate on NAFLD in the zebrafish model. The NAFLD model was induced in zebrafish by administering thioacetamide. Experimental groups were established, including a normal control group, the model control group, the positive control group, the nutraceutical complex intervention group, and the choline bitartrate alone intervention group. The intervention was administered to the zebrafish in a water-soluble form, while the positive control group received polyene phosphatidylcholine at a concentration of 50.0 μg/mL. Notably, the protective effect of the nutraceutical complex against NAFLD is more pronounced than that observed with choline bitartrate supplementation alone. The results of transcriptomics and quantitative real-time PCR showed that the potential mechanisms underlying the effects of the nutraceutical complex might involve the upregulation of acacia, acsl1a , fbp2 gene expression, and the downregulation of tbc1d1 gene expression. These results were further validated by western blotting and overexpression experiments. Our findings indicated that choline bitartrate, zinc citrate, and dl-α-Tocopheryl acetate can help improve NAFLD. The results of this study provide evidence for the application of the nutraceutical complex in the improvement of NAFLD.
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CHEMISTRY OF MATERIALS
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2016
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IF 9.407
Xiaofeng Fang,Yu-Mo Zhang,Kaiwen Chang,Zhihe Liu,Xing Su,Haobin Chen,Sean Xiao-An Zhang,Yifei Liu,Changfeng Wu
Control of stereochemistry plays a key role in medicinal chemistry, material and life science. As a prominent AIE luminogen, tetraphenylethene (TPE) derivatives have E/Z isomers which are challenging to separate even by HPLC. Herein, we designed oxetane-substituted TPE (TPE-2OXE) and separated pure isomers by simple column chromatography with high yields, as confirmed by mass spectrometry, IR and NMR spectroscopy. The isomerization of the two isomers can occur by photo- and thermo-activation. Importantly, (Z)-TPE-2OXE isomer solid shows bathochromic emission with a quantum yield 5 times higher than that of (E)-TPE-2OXE. The differences in emission wavelength and quantum yield are derived from distinct emission mechanisms of locally excited (LE) state emission of (E)-TPE-2OXE and charge transfer (CT) state emission of (Z)-TPE-2OXE. The two isomers are also good piezochromic luminescent materials, which have not only an obvious emission color shift but also significantly enhanced luminescence brightness by external force. In addition, (E)-TPE-2OXE solids show self-healing ability, which can crystallize spontaneously from ground amorphous state. The higher brightness of (E)-TPE-2OXE can be retained in solution, so fluorescent AIE nanodots are prepared from the two isomers. Cell-labeling experiments also show that (Z)-TPE-2OXE AIE dots have higher labeling brightness as compared to the (E)-TPE-2OXE isomer. The synthesis and distinct properties of E/Z isomers are beneficial to further development of new TPE derivatives for various applications.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2025
·
IF 3.9
Dengliang Cai,Guangxin Zhao,Wenan Tie,Ting Lei,Jiachun Feng
Polypropylene (PP) combines excellent mechanical properties with cost-effectiveness, yet its practical applications are hindered by poor thermo-oxidative resistance and slow crystallization kinetics. While conventional antioxidants and nucleating agents can mitigate these issues, increasing safety concerns over synthetic additives have spurred interest in natural alternatives. However, natural antioxidants such as vitamin E (VE) often exhibit inadequate stabilization at low loadings, and few industrially available biobased nucleating agents were developed. Here, we reported that myo-inositol (Ins), a bioderived compound, acted as a dual-functional additive, simultaneously improving both the thermo-oxidative stability and crystallization behavior of VE-containing PP. Incorporating 0.2 wt % Ins into PP stabilized with 0.1 wt % VE dramatically extended the oxidation induction time from 7.5 to 45.3 min, indicating strong synergistic antioxidant activity. Furthermore, Ins enhanced crystallization kinetics, increasing the peak crystallization temperature by 5.4 °C (10 °C/min cooling rate) and reducing the half-crystallization time by 69% during isothermal crystallization at 120 °C compared to VE-only PP. The dual role of Ins was attributed to its function as a metal chelator and a heterogeneous nucleation agent, offering a sustainable strategy for high-performance PP modification.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
He Zhao,Chu Xu,Caobin Zhou,Ling Zhang,Chunzhong Li
Existing triboelectric nanogenerators (TENGs) for wearable sweat sensors are plagued by low power density, which restricts their practical application. This study focuses on boosting TENGs output power via a fluorinated bi-layer electronic textile (e-textile) fabricated by electrospinning rotary collection, enabling self-powered dual functionality: real-time electrochemical sweat sensing and human motion detection. The key design includes a PCLU-HFDD negative triboelectric layer (molecularly incorporated with hexadecafluorodecanediol to enhance electron affinity) and a poly(lactic- co -glycolic acid) (PLGA) positive layer. Rotary collection creates vein-like nanostructures on PCLU-HFDD yarns (6.2 μm roughness, 114.8° water contact angle), increasing contact area. The PCLU-HFDD TENG achieves a high power density of 0.66 W m −2 , an open-circuit voltage (Voc) of 117.08 V, and excellent stability over 35,000 contact-separation cycles. Critically, integrated with both sweat-sensing and motion-detection capabilities, the e-textile enables 180-min continuous glucose monitoring (under sedentary conditions) and recognition of human motions. This work confirms that the power enhancement not only overcomes the bottleneck of TENGs for wearable sensors but also facilitates dual-modal health monitoring, advancing the development of practical wearable bioelectronics.
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Biomolecules
·
2026
·Vol. 16
·期 1
·152
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IF 4.8
Zeyan Xu,Chuyu Qin,Ruohan Zhang,Mengting Wu,Anqi Cui,Wei Chen,Lu Chen,Daqing Gao,Ruihua Shi
Adherent-invasiveE. coli(AIEC) is closely related to inflammatory bowel disease (IBD). However, its pathogenic mechanism has not yet been fully elucidated. Using a BLASTP search, we discovered that the amino acid sequence of a putative protein (UFP37798.1) in the AIEC LF82 strain is highly homologous to some regulators in the SlyA family. We named it EruA. We displayed the secondary structures of EruA using bioinformatics, overexpressed the His6-tagged EruA protein using SDS-PAGE, and dissected the genetic organization of theeruAchromosomal region using 5′RACE. We constructed aneruAdeletion mutant (ΔeruA) and a complementary strain (CΔeruA) of the LF82 strain. The transcriptomes of wild-type (WT) and ΔeruA bacteria were compared using RNA sequencing and qRT-PCR, thereby identifying 32 differentially expressed genes (DEGs). Based on YASARA software and EMSA analysis, EruA directly binds to the consensus sequences (PfimAand PtnaB) in the promoter region of thefimAandtnaBgenes from these DEGs. By using a super-resolution confocal microscope (SCM), counting CFUs of colonies on plates, indole quantification, and crystal violet staining of biofilms adhered to tubes or 96-well plates, we found that EruA activates thefimAto promote bacterial adhesion to intestinal epithelial cells and activates thetnaBto enhance bacterial indole production and biofilm formation. Moreover, EruA helps AIEC resist environmental stress and enhances bacterial survival within macrophages as well as loading in mouse tissues. Notably, EruA promotes AIEC colonization in the colons of mice and exacerbates intestinal inflammation caused by bacterial infection in mice with DSS-induced inflammatory colitis, manifested by weight loss, colon length shortening, and pathological changes in colon tissues. Therefore, EruA plays a key role in the pathogenicity of AIEC.
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ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
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2026
·
IF 3.5
Margarita A. Arbuzova,Olga Yu Selyutina,Nikolay E. Polyakov
Cholesterol drives E-protein depth redistribution via a matrix-mediated helix tilt adjustment. • Escin-induced peptide localization shifts strictly require the presence of charged DMPS lipids. • Escin is hypothesized to modulate the viroporin conformation via localized helix kinking rather than matrix disruption. • Escin is able to extract cholesterol from lipid membrane. β-Escin is a well-known saponin derived from the horse chestnut tree. Saponins, including escin, exhibit a wide range of biological activities, among which membrane-modifying activity is particularly notable. The latter is key to understanding the molecular mechanisms underlying escin's biological effects, including its antiviral properties. For example, altering membrane properties can strongly influence the life cycle of enveloped viruses such as SARS-CoV-2 by modulating the activity of its envelope protein (E protein). In the present paper, we studied the localization of both escin and the E protein transmembrane domain (ETM) in model membranes with different lipid compositions. We demonstrated that escin can alter ETM localization in membranes containing the negatively charged lipid DMPS, and that escin is also capable of extracting cholesterol from model lipid membranes. Download: Download high-res image (423KB) Download: Download full-size image
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Frontiers in Chemistry
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2026
·
IF 5.3
Pengfei Yang,Lingqi Kong,Qiongbo Wang,Qiang Liu,Xiujin Duan,Chen Hu,Zhengbo Feng,Qi Yang,Huabo Jv
Introduction To investigate the impact of different processing methods on the volatile components in Aglaia odorata flower extract (AOFEs). Methods Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry and electronic nose (E-Nose) analysis were employed to characterize volatiles of extracts obtained by ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), and heated reflux extraction (HRE). Multidimensional assessment using aroma radar charts, orthogonal partial least squares-discriminant analysis (OPLS-DA), K-means clustering, and relative odor activity value (ROAV) revealed significant processing-dependent variations. Results and discussion The results indicated that the fiber coated with DVB/CAR/PDMS had optimal extraction efficiency. A total of 46 compounds were identified, including eight alcohols, four aldehydes, one acid, 25 terpenes, seven ketones, and one heterocyclic compound. UAE and MAE had 36 and 38 compounds respectively, sharing similar compositional profiles but differing in concentrations, while HRE produced only 25 compounds Sensory evaluation and E-Nose results revealed differences in the aroma profiles of the extracts, with UAE and MAE extracts exhibiting intensified floral and sweet notes, whereas HRE displayed prominent green and spicy characteristics. K-means clustering categorized volatile evolution trends into four distinct subclasses. OPLS-DA identified 13 differential volatiles with variable importance in projection greater than 1, with ROAV analysis further selecting eight key markers, including (1R,7 S )-germacra-4(15),5,10(14)-trien-1 β -ol, α -humulene, copaene, β -cadinene, (E)- β -caryophylene, Γ -cadinene, humulene oxide II, and caryophyllene oxide. These compounds collectively contribute to the sweet and floral attributes of AOFEs. This study elucidated extraction-method-dependent volatile profiles and aroma characteristics, providing theoretical guidance for process optimization and quality enhancement in AOFEs production.
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FOOD RESEARCH INTERNATIONAL
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2026
·
IF 8.8
Ke Han,Jiankang Ren,Boyan Sun,Xiaomei Wang,Qian Ran,Haiyang Jiang
A novel phage HH3 of E. coli was isolated and characterized. • A colorimetric sensor was developed for E. coli detection within 35 min. • The biosensor shows high sensitivity and accuracy in various food and water matrices. • The biosensor exhibited a wide linear range and a low LOD of 3.2 CFU/mL. • It distinguishes viable E. coli from dead cells, beyond conventional methods. A phage HH3-functionalized Ce-UiO-66-NH₂ nanozyme-based colorimetric biosensor was developed for the quantification of viable Escherichia coli ( E. coli ), overcoming the drawbacks of slow detection and inadequate live/dead bacterial discrimination in conventional analytical methods. The proposed platform achieves target detection within 35 min, with a low limit of detection (LOD) of 3.2 CFU/mL and satisfactory spiked recoveries ranging from 82.75% to 122.06%. Mechanistically, Ce-UiO-66-NH₂ exhibits intrinsic oxidase-like activity that directly catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-colored oxTMB. The color intensity of this chromogenic reaction is positively correlated with E. coli concentration, enabling quantitative analysis. Benefiting from the specific recognition capability of phage HH3, the biosensor shows negligible responses to non-host bacteria and heat-inactivated E. coli . This distinctive advantage cannot be achieved by antibody- or PCR-based strategies. This nanozyme-phage platform outperforms most reported sensors and possesses promising potential for rapid and selective quantification of viable E. coli in complex food and environmental matrices. Download: Download high-res image (252KB) Download: Download full-size image
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Journal of Mechanical Science and Technology
·
2026
·
IF 2.2
Khalil Muhammad Kaleem Ullah,Gul Rizwan M.,Shah Nouman Ali,Jan Syed Rooh Ullah,Ahmad Adnan,Kamran M. Ali,Xu Jia-Zhuang,Li Zhong-Ming
Wear and oxidation resistance are among the primary requirements for successful and sustainable total joint replacements (TJRs). Both of these factors are major causes of TJRs failure. To address these issues and enhance the service life of TJRs, the effects of vitamin E (VE) and vitamin E-D-sorbitol (VE-DS), which are antioxidants (AOs), on stabilizing ultrahigh-molecular-weight polyethylene (UHMWPE) are presented. These antioxidants were blended with control-UHMWPE at a concentration of 0.3 % by weight and consolidated by compression molding. Wear occurs on the surface, and restricting the crosslinking to a layer on the surface is desirable, as bulk crosslinking reduces the toughness and fatigue strength of the implant. In this study, surface chemical crosslinking of control-UHMWPE, VE-UHMWPE and VE-DS-UHMWPE with dicumyl peroxide (DCP) is presented. The crosslinked density, crystallinity, wear volume, mechanical performance, biocompatibility and oxidation stability assessed by oxidation induction time (OIT) of the UHMWPE blends were characterized through different techniques. The control-UHMWPE blend showed the highest wear resistance but the lowest OIT, while the VE-DS-UHMWPE blend displayed highest crystallinity, best mechanical performance and the highest OIT, exhibiting the synergistic effect of vitamin E and D-sorbitol on the oxidation resistance of UHMWPE.
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Advanced Sustainable Systems
·
2019
·Vol. 3
·期 5
·1900026
·
IF 0
Yanyan Wang,Xue Yu,Gen Liu,Xiaodong Zhu,Rui Xu,Mei Ji,Yanqing Ma,Lei Ma
Proton exchange membrane water electrolysis (PEMWE) is known as an effective and environmental-friendly method for hydrogen production. However, the process is hindered by the necessity of large loading of high-priced noble metals in the conventional approaches. To resolve this problem, a highly efficient Nafion-based catalyst and proton exchange system are developed, which only need ultralow platinum loading by direct e-beam evaporation deposition. Scanning electron microscopy is performed before and after the deposition, which reveals a very smooth morphology. By assembling the PEMWE device with above-mentioned cathode and conventional iridium black anode, the current density reaches 500 mA cm −2 with a platinum loading of only 0.00379 mg cm −2 at 1.64 V under 80 °C. A mass activity of ≈132 A mg −1 is achieved, which is one order of magnitude higher than previously reported results. Moreover, powered by a commercial silicon solar cell, it realizes a solar-to-hydrogen conversion efficiency up to 8.26%. This work opens up an avenue of fabricating PEMWE with an ultralow catalyst loading, which may have enormous potential for future industrial scale applications.
研究领域:
能源材料
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电化学
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氢气生产
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薄膜沉积
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