Natural Products and Bioprospecting
·
2024
·Vol. 14
·期 1
·1-16
·
IF 4.8
Yang Ya-Ya,Jing Shun-Li,Shao Jia-Li,Chen Ji-Xuan,Zhang Wei-Feng,Wan Si-Yuan,Shen Yu-Ping,Yang Huan,Yu Wei
Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl- γ -aminopropyl trimethoxy (R). R-SBA-15 was employed to purify and immobilize recombinant β -glucosidase from Terrabacter ginsenosidimutans (BgpA) in one step for the first time. Optimum pH of the constructed R-SBA-15@BgpA were 7.0, and it has 20 ℃ higher optimal temperature than free enzyme. Relative activity of R-SBA-15@BgpA still retained > 70% at 42 ℃ after 8-h incubation. The investigation on organic reagent resistance revealed that the immobilized enzyme can maintain strong stability in 15% DMSO. In leaching test and evaluation of storage stability, only trace amount of protein was detected in buffer of the immobilized enzyme after storage at 4 ℃ for 33 days, and the immobilized BgpA still maintained > 50% relative activity. It also demonstrated good reusability, with 76.1% relative activity remaining after fourteen successive enzymatic hydrolyses of epimedin A to sagittatoside A. The newly proposed strategy is an effective approach for the purification and immobilization of BgpA concurrently. In addition, R-SBA-15@BgpA was demonstrated to have high efficiency and stability in this application, suggesting its great feasibility and potential to produce bioactive compounds such as secondary glycosides or aglycones from natural products. Graphical Abstract
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RADIOCHIMICA ACTA
·
2018
·Vol. 106
·期 9
·709-717
·
IF 1.202
Junhua Luo,Li Jiang,Long He
The (n,2n) cross sections and their isomeric cross-section ratios (σ m /σ g ) in the neutron energy range 13–15 MeV have been measured for 85,87 Rb by an activation and off-line γ-ray spectrometric technique using the Pd-300 Neutron Generator at the Chinese Academy of Engineering Physics (CAEP). The natural Rb samples and Nb monitor foils were activated together to determine the reaction cross section and the incident neutron flux. The neutrons were produced via the 3 H( d , n ) 4 He reaction. The pure cross section of the ground-state was derived from the absolute cross section of the metastable state and the residual nuclear decay analysis. The 85 Rb(n,2n) 84m,g Rb and 87 Rb(n,2n) 86m,g Rb reaction excitation functions and their isomeric cross-section ratios were also calculated theoretically using the TALYS-1.8 code with different level density options. Results are discussed and compared with the corresponding literature data.
研究领域:
核物理学
·
放射化学
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FUEL
·
2026
·
IF 7.8
Xingping Kai,Zhaowei Liu,Tianhua Yang,Tao Zhang,Bingshuo Li,Shiyu Feng,Rundong Li
The development of efficient metal-modified core–shell molecular sieve catalysts for the catalytic co-pyrolysis of lignin and plastics to produce aromatics is an effective way to achieve high-value utilization of waste materials. In this study, x-Fe@HZSM-5/SBA-15 (x-Fe@H/S) catalysts (x represents Si/Al) of HZSM-5 cores with Fe-Si-Al as the backbone were synthesized by isomorphous substitution and covered with an SBA-15 shell. The effects of the Si/Al ratio and Fe isomorphously substituted Al in the catalysts on the product distribution, and the formation characteristics of aromatics were investigated during the co-pyrolysis of dealkalized lignin (DL) and high-density polyethylene (HDPE). The catalyst’s cyclic regeneration performance was also analyzed. The results indicated that the pore size and acidic site distribution of the catalysts could be regulated by the Si/Al ratio, thereby promoting aromatics formation. Among them, 50-Fe@H/S exhibited the best performance, with high activity, appropriate mesopore size, and moderate acidity (0.25 mmol/g), achieving the highest bio-oil content (20.54 wt%) and aromatics content (25.96 area%). Meanwhile, the regenerated 50-Fe@H/S maintained satisfactory catalytic performance. Based on the experimental results, the aromatics formation pathways during catalytic co-pyrolysis over x-Fe@H/S were proposed, and the effects of Fe incorporation were analyzed using density functional theory.
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INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Pengchao Qiao,Ao Zhang,Rui Sun,Wei Wang
This work presents a simple P123-based quaternary templating system using titanyl sulfate (TS) as the TiO2 precursor and self-contained sulfuric acid as the catalyst (TS/TEOS/P123/H2O). A unique structural configuration of SBA-15-type mesopore frameworks embedded with high-content TiO2 nanoparticles can be directly obtained. Even with a high TiO2 content (29.1 wt %), well-defined mesostructures free of pore blocking can be secured. A new structural formation mechanism is unveiled: a self-assembly process between inorganic species and P123 micelles yields ordered mesostructures catalyzed by self-contained TS in the low-temperature step, while sol–gel reaction and crystallization of TS coincide with processes of mesostructural re-organization and partial evacuation of P123 from mesopores. The incorporation of high-content TiO2 nanoparticles into mesopore frameworks mainly happens during the hydrothermal treatment step. Not surprisingly, thanks to well-defined mesostructures containing high-content accessible TiO2 nanoparticles, such TiO2/SBA-15 composites show high activity and good reusability in photodegrading Rhodamine B and humic acids and photoreducing highly toxic Cr6+ in water under UV irradiation.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2021
·
IF 3.764
Yufei Huang,Xiaowen Zhang,Xiao Luo,Hongxia Gao,Zain Ali Saleh Bairq,Paitoon Tontiwachwuthikul,Zhiwu Liang
The huge energy consumption of rich amine solution regeneration severely restricts the large-scale application and promotion of the CO2 capture process by the amine method. In order to reduce the operating energy consumption during the CO2 capture process using amines, especially the energy consumption of absorbent regeneration, zeolite β/SBA-15 (BS) has been synthesized in this work using the hydrothermal method with zeolite beta (β) as the silicon source; β was used to prepare the novel Zr@BS and Fe–Zr@BS catalysts for amine regeneration. Experiments for CO2 stripping were performed at 370.15 K using amine solvent [monoethanolamine (MEA)] with an initial CO2 loading of 0.50 mol CO2/mol amine. Additionally, all the materials were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, N2 adsorption–desorption experiment, ammonia temperature-programmed desorption, and pyridine adsorption infrared spectroscopy. Also, the catalytic CO2 desorption performances of seven different catalysts (β, SBA-15, three BS catalysts, Zr@BS, and Fe–Zr@BS) were investigated and assessed from the aspects of the cyclic capacity, desorption rate, and energy consumption. Experimental results indicated that the Fe–Zr@BS catalysts exhibited superior catalytic behaviors than other catalysts studied in this work, improving the desorption factor of MEA solution by 212% and bringing down the energy heat by 33% compared with 5 M MEA solution without catalysts. Furthermore, the Fe–Zr@BS catalysts show good stability and easy regeneration and do not influence absorption performance of the amine solution. Moreover, the possible catalytic mechanism for amine regeneration was proposed, and the stability of the material in terms of the structure of the material and the regeneration heat duty were also studied.
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JOURNAL OF MOLECULAR LIQUIDS
·
2019
·
IF 4.561
Wenbo Zhu,Yongzhong Jia,Jinhe Sun,Pengrui Zhang,Fei Shao,Yan Jing
A novel synergistic extraction system was studied for the separation of lithium isotopes using lithium salts solution as aqueous phase and dichloromethane containing 4′-acetylbenzo-15-crown-5 and [BMIm][NTf 2 ] as organic phase with solvent extraction method . The key factors such as anions of lithium salts, concentrations of LiNTf 2 , initial pH values of aqueous phase, solvents, volume ratio of dichloromethane and [BMIm][NTf 2 ] were investigated in this study. Results showed that the maximum extraction rate of lithium was 68.36% and the maximum single stage separation factor of 6 Li and 7 Li was 1.056 ± 0.002. The formed complex in organic phase was studied by the slope analysis method, which indicated that the complex was composed of Li-ion and 4′-actylbenzo-15-crown-5 by 1:1. The thermodynamic parameters of the 6 Li and 7 Li exchange reaction were calculated by data obtained on temperature during experiments, which explained that the exchange behavior of 6 Li and 7 Li in this system was a spontaneous exothermic reaction . In addition, this system was able to efficiently separate lithium isotopes at lower temperature.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xin Xiong,Yao Zhang,YinFang Wang,HaiFeng Sha,Nengqin Jia
By virtue of the metal organic framework UiO-66-NH 2, an ingenious electrochemiluminescence immunosensor was successfully constructed for subtle exploration of the breast cancer biomarker CA 15-3. In this protocol, Ru(bpy) 3 Cl 2 ·6H 2 O (Ru(bpy) 3 2+ ), as a conventional luminescent reagent, provides a stable and strong luminescent signal. The UiO-66-NH 2 serves as a carrier to provide a large specific surface area for enhancing the electrochemical luminescence signal response of Ru(bpy) 3 2+ . UiO-66-NH 2 , Ru(bpy) 3 2+ are fixed together with Nafion membrane, and CA 15-3 antibody is covalently conjugated onto the membrane through the amide reaction. Ru(bpy) 3 2+ @UiO-66-NH 2 is used as the detection system. The experimental results reveal that the ECL signal decreased obviously after the CA 15-3 antigen molecule is captured on the immunosensor, with the capture of manifesting the principle of electrochemical luminescence quenching detection. Under optimal conditions, the sensor is sensitive and selective for detecting CA 15-3 with the detection range from 5 × 10 −4 to 5 × 10 2 U mL -1 and the low LOD (1.7705 × 10 -5 U mL -1 ). Furthermore, multiple experiments elucidate that the as-prepared sensor could be used for susceptibly detect CA 15-3 in actual samples, which has underlying application prospects in biological analysis.
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Polymers
·
2017
·Vol. 9
·期 12
·653
·
IF 3.364
Chenghong Sun,Jinhua Wang,Jiaojiao Huang,Dandan Yao,Chong-Zhi Wang,Lei Zhang,Shuying Hou,Lina Chen,Chun-Su Yuan
The feasible, reliable and selective multi-template molecularly imprinted polymers (MT-MIPs) based on SBA-15 (SBA-15@MT-MIPs) for the selective separation and determination of the trace level of ginsenoside Rb1(Rb1), ginsenoside Rg1(Rg1) and notoginsenoside R1(R1) simultaneously from biological samples were developed. The polymers were constructed by SBA-15 as support, Rb1, Rg1, R1as multi-template, acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The new synthetic SBA-15@MT-MIPs were satisfactorily applied to solid-phase extraction (SPE) coupled with high performance liquid chromatography (HPLC) for the separation and determination of trace Rb1, Rg1and R1in plasma samples. Under the optimized conditions, the limits of detection (LODs) and quantitation (LOQs) of the proposed method for Rb1, Rg1and R1were in the range of 0.63–0.75 ng·mL−1and 2.1–2.5 ng·mL−1, respectively. The recoveries of R1, Rb1and Rg1were obtained between 93.4% and 104.3% with relative standard deviations (RSDs) in the range of 3.3–4.2%. All results show that the obtained SBA-15@MT-MIPs could be a promising prospect for the practical application in the selective separation and enrichment of tracePanax notoginsengsaponins (PNS) in the biological samples.Keywords:multi-template molecularly imprinted polymers;SBA-15;Panax notoginsengsaponins;separation and determination;solid-phase extraction
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JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Shun Zhang,Ting Cai,Jing Lin,Jia-Juan Liu,Yong-Gang Zhao,Min-Yi Cao
Because of the “enterohepatic circulation” of bile acid, liver damage can be reflected by monitoring the content of bile acid in the serum of the organism. To monitor the concentration of 15 bile acids in plasma samples, a new technique of PRiME (process, ruggedness, improvement, matrix effect, ease of use) pass-through cleanup procedure combined with high performance liquid chromatography-tandem quadrupole mass spectrometry (HPLC-MS/MS) was developed. The sorbent used in the PRiME pass-through cleanup procedure is a new type of magnetic organic resin composite nano-material modified by C18 (C18-PS-DVB-GMA-Fe 3 O 4 ), which has high cleanup efficiency of plasma samples. It also shows good performance in the separation and analysis of 15 kinds of bile acids. Under the optimal conditions, the results show higher cleanup efficiency of C18-PS-DVB-GMA-Fe 3 O 4 with recoveries in the range of 82.1–115 %. The limit of quantitative (LOQs) of 15 bile acids were in the range of 0.033 µg/L-0.19 µg/L, and the RSD values of 15 bile acids were in the range of 3.00–11.9 %. Validation results on linearity, specificity, accuracy and precision, as well as on the application to analysis of 15 bile acids in 100 human plasma samples demonstrate the applicability to clinical studies.
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CHEMISTRY OF MATERIALS
·
2016
·
IF 9.407
Jun Shao,Songwang Yang,Lei Lei,Qipeng Cao,Yu Yu,Yan Liu
Pore size and porosity of the porous materials play an important role in catalysis, dye-sensitized solar cells and mesoscopic perovskite solar cells (PSC), etc. Increasing pore size and porosity of mesoporous TiO2 is crucial for facilitating pore-filling of perovskite, charge extraction on TiO2/CH3NH3PbI3 interface and thus cell performance enhancement. Highly porous TiO2 films (TFs) with a large pore size that extends the limit of particle size have been achieved through a novel TiO2 paste using copolymer P123 as a pore-adjusting agent and 2-butoxyethyl acetate as a solvent. A highly porous structure with the pore size of 34.2 nm and porosity of 73.5% has been obtained, the porosity of which is the largest that has ever been reported in the screen-printed TiO2 thick films. The pore size and porosity of TFs can be successively adjusted in a certain range by tuning the P123 content in the pastes. As particle size and surface area of TFs are kept almost constant, the specific investigation on the effect of varied pore size on the performance of bilayer-structured PSCs becomes possible. The hysteresis phenomenon, the notorious problem of PSCs, is found to depend greatly on pore size and porosity of TFs, that is, pore-filling of perovskite. The suppressing effect of highly porous TFs on hysteresis by avoiding charges accumulation on the interface due to enhanced interfacial contact is proved by the invariable photocurrent response after prebias treatment. A hysteresis-free solar cell with an efficiency of 15.47% was achieved by depositing a 242 nm-thick perovskite capping layer upon 350 nm-thick TF with a pore size of 34.2 nm. This method developed for the preparation of highly porous TFs provides a new way to fabricate hysteresis-free PSCs and is widely applicable for the fabrication of other mesoporous metal oxide films with large pore sizes.
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EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
·
2014
·Vol. 2014
·期 14
·2337-2344
·
IF 2.965
Ling-Yan Meng,Shang-Ru Zhai,Shi Li,Bin Zhai,Qing-Da An,Xiao-Wei Song
Graphical A layer-by-layer deposition strategy was used for the preparation of a tungstophosphoric acid/pentaethylenehexamine/ZrSBA-15 composite. This recyclable material was stable at high temperatures and in polar solvents, and it exhibited excellent catalytic properties (84.7 % conversion, >99 % selectivity) in the selective oxidation of benzyl alcohol (BnOH) to benzaldehyde (BzH). A layer-by-layer deposition strategy was employed for the immobilization of tungstophosphoric acid (HPW) onto a pentaethylenehexamine (PEHA)-grafted ZrSBA-15 framework to a give HPW/PEHA/ZrSBA-15 composite. The resulting composite was characterized by XRD, SEM, TEM, FTIR, thermogravimetric analysis, N 2 sorption, and X-ray photoelectron spectroscopy, and these methods showed that the composite was stable in polar environments. These properties were attributed to the method of preparation, which ensured that a dense and stable array of HPW was encapsulated within the pores of PEHA-modified ZrSBA-15. In the H 2 O 2 -mediated oxidation of benzyl alcohol, the 25 % HPW/PEHA/ZrSBA-15 catalyst exhibited high selectivity (>99 %) for benzaldehyde. Moreover, the oxidation reaction was performed under solvent-free conditions, and the catalyst was recycled several times with negligible loss in selectivity and conversion. This HPW-based catalyst was also efficient at selectively oxidizing other benzylic and alkyl alcohols to the corresponding aldehydes.
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DALTON TRANSACTIONS
·
2014
·Vol. 43
·期 37
·14123-14131
·
IF 4.097
Lei Chen,Bing Yan
Both zeolite A/L and SBA-15 have been used to assemble novel photofunctional multi-component lanthanide hybrid materials. Microporous zeolites A/L are first functionalized by embedding lanthanide or zinc complexes (TTA = thenoyltrifluoroacetone, TAA = trifluoroacetone, bipy = 2,2′-bipyridyl) into the pores of zeolite A/L, and then the surface of functionalized zeolite A/L is modified via covalent linkers from aromatic carboxylic acids (para-aminobenzoic acid ABA and para-hydroxyl benzoic acid HBA) grafted by 3-(triethoxysilyl)-propyl isocyanate (TEPIC). On the other hand, SBA-15 is modified by grafting covalent linker phen-Si from the modification of 1,10-phenanthroline (phen) with TEPIC. Further, another lanthanide ion is used to link both functionalized zeolites, A/L and SBA-15, through the coordination with the two covalent linkers, ABS-Si (HBA-Si) and phen-Si, respectively. Subsequently, the obtained multicomponent microporous–mesoporous hybrid materials are assembled and characterized using XRD, FT-IR, UV-vis DRS, SEM, TEM and luminescent approaches (spectra, lifetimes and quantum efficiencies). These hybrids with both microporous zeolite and SBA-15 mesoporous host possess favourable luminescent performance and some hybrid systems present the high quantum efficiencies of 90%. Besides, the luminescent color can be tuned by adjusting the composition of these hybrids, among which four hybrids (S-phen-Eu-HBA-[ZA-Tb-bipy], S-phen-Eu-HBA-[ZA-Zn-bipy], S-phen-Eu-ABA-[ZA-Tb-TAA], S-phen-Eu-ABA-[ZL-Tb-bipy]) can be integrated to white light emission.
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