BIOMATERIALS
·
2014
·
IF 8.312
Gan Tian,Xiaopeng Zheng,Xiao Zhang,Wenyan Yin,Jie Yu,Dongliang Wang,Zhiping Zhang,Xiangliang Yang,Zhanjun Gu,Yuliang Zhao
Multi-drug resistance (MDR) is a major cause of failure in cancer chemotherapy. Tocopheryl polyethylene glycol 1000 succinate (TPGS) has been extensively investigated for overcoming MDR in cancer therapy because of its ability to inhibit P-glycoprotein (P-gp). In this work, TPGS was for the first time used as a new surface modifier to functionalize NaYbF 4 :Er upconversion nanoparticles (UNCPs) and endowed the as-prepared products (TPGS-UCNPs) with excellent water-solubility, P-gp inhibition capability and imaging-guided drug delivery property. After the chemotherapeutic drug (doxorubicin, DOX) loading, the as-formed composites (TPGS-UCNPs-DOX) exhibited potent killing ability for DOX-resistant MCF-7 cells. Flow-cytometric assessment and Western blot assay showed that the TPGS-UCNPs could potently decrease the P-gp expression and facilitate the intracellular drug accumulation, thus achieving MDR reversal. Moreover, considering that UCNPs process efficient upconversion emission and Yb element contained in UCNPs has strong X-ray attenuation ability, the as-obtained composite could also serve as a dual-modal probe for upconversion luminescence (UCL) imaging and X-ray computed tomography (CT) imaging, making them promising for imaging-guided cancer therapy.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Linlin Zhao,Juan Li,Yuhong Liu,Yuan Wei,Jin Zhang,Jinzha Zhang,Qing Xia,Qiang Zhang,Wenbo Zhao,Xiaoqiang Chen
We reported a novel ECL immunosensor for detection of carcinoembryonic antigen (CEA) based on the reduced graphene Oxide-BaYF 5 :Yb, Er (rGO-BaYF 5 :Yb, Er) nanocomposites. The rGO-BaYF 5 :Yb, Er nanocomposites, used as electrode luminescence materials, were prepared by in situ hydrothermal method, and characterized by TEM, XRD, UV–vis spectra, and upconversion fluorescence spectra. Further, poly (diallyldimethylammonium chloride) (PDDA), Au nanoparticles (AuNPs), and Anti-CEA were successively assembled on the surface of rGO-BaYF 5 :Yb, Er nanocomposites modified-electrode for fabricating ECL immunosensor. The results of the ECL measurements of CEA indicated that this novel ECL immunosensor exhibited high sensitive response to CEA in a linear range of 0.001–80 ng mL −1 with a low detection limit of 0.87 pg mL −1 , good selectivity and satisfactory stability.
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DALTON TRANSACTIONS
·
2016
·Vol. 45
·期 27
·11129-11136
·
IF 4.177
Yue Su,Xiuling Liu,Pengpeng Lei,Xia Xu,Lile Dong,Xianmin Guo,Xingxu Yan,Peng Wang,Shuyan Song,Jing Feng,Hongjie Zhang
Core–shell–shell heterostructures of α-NaLuF4:Yb/Er@NaLuF4:Yb@MF2 (M = Ca, Sr, Ba) have been successfully fabricated via the thermal decomposition method. Upconversion nanoparticles (UCNPs) were characterized by powder X-ray diffraction (XRD), transmission electron microscopy (TEM), upconversion luminescence (UCL) spectroscopy, etc. Under 980 nm excitation, the emission intensities of the UCNPs are remarkably enhanced after coating the MF2 (M = Ca, Sr, and Ba) shell. Among these samples, CaF2 coated UCNPs show the strongest overall emission, while BaF2 coated UCNPs exhibit the longest lifetime. These results demonstrate that alkaline earth metal fluorides are ideal materials to improve the UCL properties. Meanwhile, although the lattice mismatch between the ternary NaREF4 core and the binary MF2 (M = Sr and Ba) shell is relatively large, the successfully synthesized NaLuF4:Yb/Er@NaLuF4:Yb@MF2 indicates a new outlook on the fabrication of heterostructural core–shell UCNPs.
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Materials Today Communications
·
2024
·
IF 3.7
Bingxin Yuan,Meiting Li,Chi Zhang
The RE 3+ doped YbNbO 4 and Yb 3 NbO 7 nanophosphors (RE=Ho, Er and Tm) were synthesized via a calcination-assisted hydrothermal procedure. YbNbO 4 intensified almost the whole up-conversion (UC)/down-conversion (DC) emissions of RE 3+ by ∼2 times when compared with Yb 3 NbO 7 components, indicating that a lower symmetry environment in the former case is more favorable for photoluminescence. UC luminescence in each case was dominated by a green band at ∼539 nm for Ho 3+ and a NIR band at ∼803 nm for Tm 3+ , while doping YbNbO 4 and Yb 3 NbO 7 with Er 3+ separately yielded the slightly stronger green 556 nm and red 658 nm emissions. The UC/DC luminescence mechanisms were elaborated via Yb 3+ → RE 3+ forward/backward energy transfer and cross relaxation.
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高影响因子
JOURNAL OF LUMINESCENCE
·
2024
·
IF 3.3
Wei Zhou
This paper focuses on the study of core-shell rare earth NaLuF 4 :Yb,Er@NaLuF 4 :Yb,Tm@NaLuF 4 upconversion nanoparticles (UCNPs) with multispectral emission for applications in the field of ratiometric temperature sensors. This approach lays the foundation for exploring more luminous intensity ratios (LIRs) between different spectra for temperature sensing. Additionally, it facilitates the use of different LIRs for various temperatures, yielding higher sensitivity. Under 980 nm laser excitation, these UCNPs produce multispectral luminescence covering blue, green, red, and infrared luminescence intervals. Within the temperature range of 293–473 K, we measured the luminescence intensity of different emission peaks and observed that the 803 nm peak exhibits an anomalous thermal enhancement effect. The LIR between the luminescence intensities of different emission peaks is fitted using formulas to calculate the absolute sensitivity ( S a ), relative sensitivity ( S r ), uncertainty ( ΔT ), and repeatability ( R ) of the corresponding LIR. Comparisons revealed that LIR(I 803 /I 540 ) demonstrates greater S a compared to the other LIRs. Furthermore, the S r of LIR(I 803 /I 540 ) is higher in the elevated temperature range, whereas LIR(I 522 /I 540 ) shows greater values in the lower temperature range. By combining the advantages of these two LIRs at different temperatures, LIR(I 522 /I 540 ) is utilized at low temperatures and LIR(I 803 /I 540 ) at high temperatures, achieving a combined S r of 0.67–1.06 %K −1 and a combined ΔT of less than 0.045. The R of LIR(I 803 /I 540 ) is tested and found to be greater than 99 % averaged over five cycles. In summary, the NaLuF 4 :Yb,Er@NaLuF 4 :Yb,Tm@NaLuF 4 UCNPs proposed in this paper demonstrate potential applications in the field of temperature sensing.
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JOURNAL OF LUMINESCENCE
·
2018
·
IF 2.732
Dandan Ju,Feng Song,Yingdong Han,Wenjing Cui,Feifei Song,Lisa Liu,Aihua Zhou,Xueqin Wang,Shujing Liu
Trivalent lanthanide ion (Ln 3+ ) doped UC materials can emit visible light under near-infrared (NIR) excitation due to anti-stokes shift and have received wide attention. In this paper, we presented a new strategy to synthesize the uniform and smooth microstructures (rodlike and tubular) based on hexagonal phase NaYF 4 microcrystals . Moreover, we systematically discussed the method of enhancing the upconversion (UC) luminescence intensity via co-doping the ions with different valences (Li + and Lu 3+ ) in order to tailor the environment around lanthanide ions. The UC intensities in the two microstructures were significantly improved. The results suggested the great application potential of tubes and rods of β-NaYF 4 microcrystals in anti-counterfeiting, laser media, photonic crystals , and waveguides .
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Bosnian Journal of Basic Medical Sciences
·
2018
·
IF 1.432
Jing Chen,Guo Zheng,Hu Guo,Zhong-nan Shi,Jiao Jiang,Xiao-yu Wang,Xiao Yang,Xian-yu Liu
Status epilepticus (SE) is defined as continuous seizure activity lasting more than 5 minutes. It results in neuronal cell death, mediated by endoplasmic reticulum (ER) stress response. Previously, metformin demonstrated neuroprotective effects in primary cortical neurons. In this study, we analyzed the effect of metformin on ER stress via the pro-apoptotic protein kinase RNA-like endoplasmic reticulum kinase (PERK)-eukaryotic initiation factor 2α (eIF2α)-C/EBP homologous protein (CHOP) pathway. SE was induced in rats by pentylenetetrazole. Following SE, the rats were treated with salubrinal, GSK2656157, or metformin. In a control group (normal saline) SE was not induced. CHOP, eIF2α, and PERK expression was determined by Western blot; apoptosis was analyzed by TUNEL assay. CHOP expression was significantly increased at 6 and 24 hours following SE. At both time points, eIF2α and PERK levels were also increased. At 6 hours, CHOP expression was significantly reduced in salubrinal, GSK2656157 and metformin groups versus SE group. eIF2α and PERK levels were decreased in metformin compared to SE group. eIF2α expression was markedly decreased in salubrinal versus SE group, while PERK expression was markedly reduced in GSK2656157 versus SE group. At 6 and 24 hours, the apoptosis rate was significantly increased in SE versus control group, while it was significantly reduced in salubrinal, GSK2656157, and metformin groups compared to SE group. The apoptosis rate also decreased in salubrinal group at 24 hours, although not to the extent observed in metformin group. Overall, CHOP expression and apoptosis induced by SE in rats were reduced with metformin. Further studies are required to evaluate the clinical relevance of metformin for patients with SE.
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ARCHIVES OF TOXICOLOGY
·
2018
·Vol. 92
·期 4
·1421-1434
·
IF 5.728
Jiang Shengwei,Lin Yuchun,Yao Huan,Yang Chuanli,Zhang Liyin,Luo Bing,Lei Zhao,Cao Liwei,Lin Naibo,Liu Xiangyang,Lin Zhongning,He Chengyong
Unfolded protein response (UPR) and endoplasmic reticulum (ER)-phagy are essential for cell homeostasis. Quantum dots (QDs), which have been widely used for biomedical applications, can accumulate in the kidney tissues and may cause renal dysfunction. However, the molecular mechanism of QDs-induced nephrotoxicity is still obscure. The present study was aimed to elucidate the role and mechanism of UPR and ER-phagy in QDs-induced nephrotoxicity. Herein, human embyronic kidney (HEK) cells were exposed to 15, 30, 45, and 60 nM cadmium telluride (CdTe)-QDs for 12 and 24 h. And CdTe-QDs (30–60 nM) inhibited the HEK cell viability. The clathrin-dependent endocytosis was determined as the main pathway of CdTe-QDs cellular uptake. Within cells, CdTe-QDs disrupted ER ultrastructure and induced UPR and FAM134B-dependent ER-phagy. Blocking UPR with inhibitors or siRNA rescued the FAM134B-dependent ER-phagy, which was triggered by CdTe-QDs. Moreover, suppression of UPR or FAM134B-dependent ER-phagy restored the cell vability. In vivo, mice were intravenously injected with 8 and 16 nmol/kg body weight CdTe-QDs for 24 h. Kidney was shown as one of highest distributed organs of CdTe-QDs, resulting in renal dysfunction, as well as UPR and FAM134B-dependent ER-phagy in it. Thus, for the first time, we demonstrated that ER-phagy can be triggered by nanomaterials both in vitro and in vivo. In addition, blocking of UPR and ER-phagy showed protective effects against CdTe-QDs-induced toxicity in kideny cells. Notably, a secreted alkaline phosphatase reporter gene system has been developed as a sensitive and rapid method for evaluating the ER quality under the exposure of nanomaterials.
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Nanoscale
·
2017
·Vol. 10
·期 6
·2834-2840
·
IF 7.367
Bin Zhou,Bing Xu,Huimei He,Zhanjun Gu,Bing Tang,Ying Ma,Tianyou Zhai
We report the enhancement of green upconversion luminescence in tetrahedral LiYF4:Yb/Er nanoparticles by Mn2+ ion doping, which is different from the enhanced single-band red emission dominated by Mn2+ ions in cubic NaLnF4:Yb/Er (Ln = Y, Gd, Lu) nanoparticles. The energy levels of the first excited state 4T1 of Mn2+ in tetrahedral LiYF4:Gd and cubic NaGd(Y)F4 are compared by detection of emissions from Mn2+via the energy transition from Gd3+ to Mn2+ with excitation at 275 nm. The coordination environments of Mn2+ in these two host lattices have been investigated by X-ray absorption fine structure measurements. The results demonstrate that the formation of tetrahedral MnF4 in tetragonal LiYF4 arising from the replacement of Ln3+ ions with Mn2+ ions leads to a higher energy level of the Mn2+ 4T1 state than that in octahedral MnF6 in cubic NaYF4. The high-lying excited state of tetrahedral MnF4 is close to the green emitting 4S3/2 state of Er3+ and thus enhances green upconversion emission in tetragonal LiYF4:Yb/Er, while the low-lying excited state of octahedral MnF6 dominates red emission in cubic NaYF4:Yb/Er. These findings provide direct evidence for the key roles of the host lattices and more possibilities in modulating the upconversion behaviour of lanthanide-based nanoparticles by transition-metal ion doping to achieve the desired goals of specific applications.
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Analytical Methods
·
2017
·Vol. 9
·期 22
·3296-3301
·
IF 1.9
Lei Wang,Yuan Ji,Yuezhen He,Lun Wang,Hongqi Chen
Li+-Doped NaYF4:Yb,Er upconversion luminescent nanoparticles (UCLNPs) were synthesized and modified with poly(acrylic acid) (PAA). The luminescence of the carboxyl-functionalized UCLNPs at 664 nm was quenched in the presence of cupric ions (Cu2+) due to the coordination of Cu2+ with the carboxyl group of PAA. When pyrophosphate ions (PPi) were added, the luminescence of the UCLNPs was restored due to the competitive binding of PPi with Cu2+. However, pyrophosphatase (PPase) could promote the hydrolysis of PPi, thus releasing Cu2+, leading to requenching of the luminescence. Under optimal conditions, the luminescence response was virtually proportional to the activity of the PPase between 0.500 and 50.0 mU mL−1, and the limit of detection was 0.441 mU mL−1. Based on the competition assay approach, a new highly sensitive method in the red region for real-time detection of PPase activity was developed.
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RSC Advances
·
2017
·Vol. 7
·期 43
·26633-26639
·
IF 3.108
Chenxi Song,Shubiao Zhang,Quan Zhou,Lei Shi,Linying Du,Defu Zhi,Yinan Zhao,Yuhong Zhen,Defeng Zhao
β-NaYF4:Yb,Er upconversion nanoparticles (UCNPs) were synthesized by a one-pot solvothermal method. The UCNPs were characterized through X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to prove that they possessed hexagonal crystal phase with uniform particle size distribution. The cationic solid lipid nanoparticles (CSLNs) were then obtained by coating the UCNPs with a lipid. Further analysis by FTIR, XRD and TEM indicated the lipid can successfully coat UCNPs, and the coating process had no obvious influences on morphology and crystallization of the UCNPs. Furthermore, the CSLNs were used for bioimaging in cells and delivering small interfering RNA (siRNA). Cellular bioimaging and upconversion luminescence studies clearly demonstrated that the CSLNs could effectively enter into cells with the help of the lipid. Silencing assays and cytotoxicity in A549 cells convincingly showed that the CSLNs could effectively deliver siRNA and possessed low cytotoxicity and good biocompatibility. Thus, the bifunctional CSLNs could be used as a potential candidate in bioimaging and therapeutic applications.
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Nano Today
·
2020
·
IF 16.907
Feng Ren,Hanghang Liu,Hao Zhang,Zhilin Jiang,Bing Xia,Cécile Genevois,Tao He,Mathieu Allix,Qiao Sun,Zhen Li,Mingyuan Gao
Highly sensitive and specific discrimination of brain tumor margins from the surrounding parenchyma remains a formidable challenge. Limited by the short of photostable probes with deep tissue penetration and high efficiency of crossing the blood-brain-barrier (BBB), the development of fluorescence-guided surgery (FGS) of brain tumors was markedly constrained. Herein, we report the capability of the strong second near-infrared-IIb (NIR IIb, 1500−1700 nm) fluorescence from Er-based lanthanide nanoparticles in imaging-guided surgery of orthotopic glioma. We designed an energy-cascaded Er 3+ -Ce 3+ -A 3+ (A = Yb, Ho, Tm) system and prepared a series of NaErF 4 : [email protected] 4 @NaLuF 4 down-conversion nanoparticles (DCNPs) for optimizing the influence of NaAF 4 interlayer and Ce 3+ dopants. We modified the optimal NaErF 4 :2.5 % [email protected] 4 (0.9 nm)@NaLuF 4 DCNPs with Dye-brush polymer (Dye-BP) to facilitate 4 I 13/2 → 4 I 15/2 transition, which leads to an impressive 675-fold enhancement of 1525 nm fluorescence in aqueous solution under 808 nm excitation due to the excellent energy-cascaded downconversion (ECD), in comparison with that of NaErF 4 nanoparticles. We further modified these highly bright nanoparticles with tumor-targeting angiopep-2 peptide, and efficiently delivered them to the glioma by using the focused ultrasound sonication (FUS) to temporarily open the BBB. We obtained the highest tumor-to-background ratio (TBR = 12.5) ever reported in the targeted NIR IIb fluorescence imaging of small orthotopic glioma (size < 3 mm, depth> 3 mm) through intact skull and scalp, which was drastically improved to ∼150 after cardiac perfusion and craniotomy to ensure the precise resection of tumor. More importantly, the size of glioma measured from the width of fluorescence profile is very close to that from T 2 -weighted MRI images. Our work provides the insights into engineering NIR IIb fluorescence of lanthanide nanoparticles, and demonstrates the great potential of NIR IIb fluorescence imaging-guided surgery of tumor.
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