FOOD CHEMISTRY
·
2025
·
IF 9.8
Yutong Huang,Yan Luo,Xiaoqing Zhang,Shanglin Li,Mei Liu
The accurate detection of carbamate pesticides popularly employed in agricultural products is critical for reducing the threat of resultant residues to human health. In this work, a regenerable nanofilm used for SERS substrate was constructed by interfacially confined self-assembly incorporating CdS nanowires (CdSNWs) and Ag nanoparticles (AgNPs). The constructed AgNPs-CdSNWs/Nanofilm could significantly enhance the Raman signals of three carbamate pesticides (metolcarb, carbaryl and aldicarb-sulfone). A broad detection linear ranged 0.001–100 mg/L was achieved. The detection limits (LOD) of metolcarb, carbaryl and aldicarb-sulfone reached 0.00081 mg/L, 0.00081 mg/L and 0.00072 mg/L with coefficients of determination of (R 2 ) of 0.992, 0.991 and 0.992, percent recovery ranged 86.5–104.9 %，88.2–105.1 % and 87.0–104.5 %, respectively, and relative standard deviation (RSD) values were all less than 4.0 %. Results showed that the user-friendly reproducible nanofilm could be employed as simple-to-establish SERS platform for rapid detection of carbamate pesticides in food.
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Mengmeng Wang,Li Zheng,Fan Sun,Qingdan Ye,Pei Liang,Kun Pang,Zihong Ye,Yufeng Wang
Aptamer sensors based on surface-enhanced Raman scattering (SERS) technology have demonstrated great potential in the ultrasensitive and rapid detection of Escherichia coli ( E. coli ). Herein, this paper presents a digital SERS aptamer sensor. This sensor integrates ordered nanoscale array synthesis technology and digital analysis technology, enabling highly sensitive and rapid bacterial quantification. The ordered monolayer gold nanosphere arrays (Au NS) can form uniform and dense “hot spots” on the silicon wafer due to their uniform spherical structures and narrow gaps. Moreover, digital SERS is adopted to further optimize the signal uniformity so as to achieve precise quantification. The sensor modules are combined together through base pairing. The aptamers labeled with Raman tags are detached from the complementary DNA due to the competition of the target substance, thus realizing the detection of E. coli . The digital SERS aptamer sensor has been verified to possess excellent selectivity and reproducibility. It has a wide dynamic linear detection range from 1.0 * 10 1 to 1.0 * 10 9 CFU/ml and a detection limit of 0.657 CFU/ml, maintaining excellent specificity even in the presence of mixed bacterial interference. The spiked recoveries in actual samples range from 98.80 % to 99.81 %. Leveraging different aptamers and digital analysis, the sensor holds promise for food safety and environmental monitoring applications.
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PubMed
高影响因子
POLYMER BULLETIN
·
2021
·Vol. 79
·期 8
·6409-6429
·
IF 2.87
Ding Guanjun,Wang Ao,Shi Xinming,Li Jumei,You Lijun,Wang Shaoyun
Polyphosphazene microspheres (PZS) multispectral encoded by fluorescence and surface enhanced Raman spectra (SERS) were prepared and used as solid phase carrier of capture antibody for antibody detection. A variety of fluorescent encoded polyphosphazene microspheres (FPZS) incorporating single and multiple fluorescent molecules were prepared. Multiple SERS encoded polyphosphazene microspheres (SPZS) were obtained by modification of different Raman reporters on the surface of PZS@Ag, which were fabricated with rough morphology through in situ deposition of Ag nanoparticles. Fluorescence and SERS spectral combined encoded polyphosphazene microspheres (FSPZS) were prepared by encoding of fluorescent molecules and SERS reporters. The optical signals in the two independent detection channels were separable and ensured the accuracy for the encoding method. Immunoassays of goat IgG, rabbit IgG and mouse IgG were performed by the as-prepared FPZS, SPZS and FSPZS as solid phase carrier of capture antibody and fluorescent labeled second antibody, using sandwich immunoassay method. The immunoassay results showed that the multiple-spectra encoded microspheres have stable and distinguishable encoding signals, large encoding volume and multiplexed analytical capability in sandwich immunoassays.
研究领域:
材料科学
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分析化学
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生物传感器
·
免疫学
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qian Liu,Xiaoying Yang,Lisha Xiang,Xiang Li,Chao Kang,Dongmei Chen
Alternariol (AOH) is a secondary toxin produced by the metabolism of Alternaria, which poses a significant food safety risk due to its widespread presence in fruits and vegetables. In this study, a dual-mode sensor based on AOH aptamer was developed, which combined with surface-enhanced Raman scattering (SERS) and fluorescence (FL) detection technology to achieve sensitive and specific detection of AOH. The sensor significantly improves the reliability of detection through the synergistic change and mutual verification of SERS and FL signals. The linear detection range of SERS mode was 0.5–100 ng/mL ( R 2 = 0.9927), and the detection limit was as low as 0.064 ng/mL. The linear range of FL mode was 1–100 ng/mL ( R 2 = 0.9792), and the detection limit was 0.14 ng/mL. Experiments showed that the sensor could effectively distinguish AOH from other mycotoxins, and showed good recovery (SERS: 84.09 % -101.40 %, FL: 4.69 % -105.57 %) and precision (RSD < 6.3 % and 8.9 %, respectively) in complex matrices such as kiwifruit, tomato and citrus. The results were consistent with those of the traditional LC-MS method, which verified its reliability. The sensing platform constructed in this study not only provides a reliable technical means for the trace detection of AOH in agricultural products, but also opens up a new way for the rapid screening and quantitative analysis of other mycotoxins in food matrices.
研究领域:
食品科学与技术
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分析化学
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纳米技术
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生物传感器
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霉菌毒素学
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Zhuang Bo,Wei Yang,Qi Wang,Chao Song,Xun Gao
Deoxynivalenol (DON) is a secondary metabolite produced by fungi that contaminates food during the process of production and storage, and can potentially cause acute or chronic poisoning upon ingestion. This study employs surface-enhanced Raman spectroscopy (SERS) combined with density functional theory (DFT) to detect the pure DON sample. The investigation focuses on the adsorption mechanism of DON molecules on gold nanoparticles (AuNPs) and indicates that the O6 atoms in the DON molecule act as sensitive sites for nucleophilic reactions by using DFT calculations. Experimental results demonstrate that pure DON molecules exhibit a strong SERS response, displaying excellent linear and non-linear relationships across concentration ranges of 0.01–0.2 μg/mL (R 2 = 0.992) and 0.2–1 μg/mL (R 2 = 0.997), respectively. The limit of detection (LOD) and quantification (LOQ) are 0.0085 μg/mL and 0.0256 μg/mL, respectively, which show excellent detection sensitivity. These findings can provide a theoretical foundation for monitoring DON toxins in foodstuffs.
研究领域:
分析化学
·
食品安全
·
纳米毒理学
·
分子互作
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光谱学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Haoqi He,Wenyun Guan,Mingchuang Zhao,Jianxiang Gao,Xudong Liu,Yichao Bai,Yu Lei
Molecular sensing is of great importance in the field of food safety, as food safety constitutes the fundamental baseline for safeguarding public health. However, the application of surface-enhanced Raman spectroscopy (SERS) in this domain remains challenging due to significant fluctuations in signal intensity when low-concentration harmful additives are detected in food samples. To address this, we propose a digital SERS approach based on a two-dimensional (2D) flat WS2 monolayer and Poisson distribution statistics. This method utilizes a plasma-treated WS2 monolayer as a highly sensitive SERS substrate and was validated through the digital SERS detection of crystal violet (CV) in both single and mixed solutions. The results demonstrate that the detection limit for CV can reach as low as 1 × 10–18 M, with no interference observed in mixed solutions, indicating excellent quantitative detection capability. Furthermore, this strategy was applied to detect synthetic colorants in black tea, where it also exhibited outstanding sensitivity and accuracy. Specifically, the limits of detection for sunset yellow and erythrosine are 10–14 M and 10–18 M, respectively. In summary, by combining a highly uniform 2D SERS substrate with digital SERS technology, this study not only expands the application scope of 2D SERS substrates but also enhances their potential for the high-precision and efficient quantitative analysis of multiple analytes in complex food samples.
研究领域:
食品安全
·
分析化学
·
纳米材料
·
传感器
·
二维材料
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DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2025
·
IF 6.4
Xinyu Miao,Xiawei Xu,Li Sun,Xiaoxia Wu,Aochi Liu,Pan Fu,Pengtao Chen,Yujiao Xie,Jiabao Guo,Zhiwei Hou,Dong Xu,Aiguo Wu,Jie Lin
The precise breast cancer treatment heavily relies on the accurate identification of therapeutic markers. Single surface-enhanced Raman spectroscopy (SERS) bioprobes have been demonstrated for tumor primary screening. However, single biomarker detection is insufficient due to tumor heterogeneity and drug resistance with cancer progression. In this study, we have developed a dual-targeted SERS sensing platform using IO NPs@AR@PDA@aPD-L1 and Au NPs@4-MBA@PDA@aHER2 bioprobes for simultaneous detection of dual therapeutic markers (PD-L1 and HER2) in breast cancer, achieving single-cell resolution imaging with a detection limit of 2 cells/mL. Specifically, by digitizing SERS spectra using machine learning, the dual-targeted strategy improved spatial resolution and effectively distinguished the heterogeneous expression of HER2 and PD-L1 in SK-BR-3, MCF-7, and MDA-MB-231 cell lines, improving detection accuracy by ~20% compared to single-marker methods. In the temporal dimension, transforming growth factor-β (TGF-β) was used to induce epithelial-mesenchymal transition (EMT) in MCF-7, simulating the highly invasive and metastatic characteristics of advanced tumors, thereby enabling dynamic monitoring of cancer progression from locally advanced to metastatic disease. This continuous monitoring capability of the dual-targeted strategy offers critical insights into tumor evolution, metastasis risk assessment, and recurrence prediction, thereby playing a vital role in guiding precision cancer management and treatment adaptation.
研究领域:
肿瘤学
·
纳米生物技术
·
分子成像
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精准医学
·
生物医学工程
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Bing Qi,Zhao Qi,Lingling Qiu,Chenjie Zhang,Jiayi Chen,Yurong Wei,Jie Wang,Minmin Xu
Lanthanide coordination nanoparticles (Ln-CNPs) have shown significant potential in optical biosensing. However, the conventional self-assembly of lanthanides and ligands generally produces large cross-linked coordination networks with uncontrollable morphologies and poor dispersibility. In this work, we developed a facile coordination-driven self-assembly strategy for preparing Ln-CNPs with tunable properties, demonstrating their exceptional utility in the dual-mode time-resolved photoluminescence (TRPL)/surface-enhanced Raman spectroscopy (SERS) detection of analytes in complex matrices. Specifically, a series of Ln-CNPs were prepared by the coordination-driven self-assembly of adenosine diphosphate (ADP) with Ln3+. The resulting Ln-CNPs exhibited a controllable morphology, excellent monodispersity, long-lived luminescence, and other functionalities. Notably, the luminescence of ADP-Eu and ADP-Tb CNPs remained detectable even at a delay time of 1 ms, making them ideal for TRPL biosensing; while ADP-Gd CNPs showed potential for magnetic resonance imaging. As a proof-of-concept for optical biosensing applications, we engineered a TRPL/SERS dual-mode platform leveraging the specific response of ADP-Eu CNPs to tetracycline (TC). TC binding to ADP-Eu CNPs induced up to 13-fold TRPL enhancement through the antenna effect, while unbound TC molecules enabled 10–9 M-level SERS detection via plasmonic coupling. Quantitative cross-verification between TRPL and SERS significantly enhanced the determination accuracy. This dual-mode platform achieved 97.64–108.90% recoveries for TC detection in serum samples with <3.2% RSD. Our methodology establishes a generalizable paradigm for engineering multifunctional Ln-CNPs and further opens exciting avenues for the accurate detection of analytes in complex biological systems.
研究领域:
材料科学
·
光学生物传感
·
纳米技术
·
分析化学
·
配位化学
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PubMed
高影响因子
Journal of Pharmaceutical Analysis
·
2025
·
IF 8.9
Zhe Ni,Xingshi Yuan,Zhengliang Luo,Xiaoqi Zhang,Min Chen,Lee Jia,Jie Wang,Xifu Shang
Sensitive detection of microRNA-204 (miR-204) is critical for the early diagnosis and management of osteoarthritis (OA). This work presents a novel surface-enhanced Raman scattering (SERS) biosensor for the ultrasensitive and specific detection of OA-associated miR-204. The platform integrates a self-amplifying nucleic acid circuit with DNAzyme-catalyzed etching of a plasmonic nanoprobe. At its core is a single, rationally designed overhang-containing hairpin probe (O-HP) that functions as both the recognition element and amplification initiator. Upon binding to miR-204, the O-HP triggers polymerase-mediated extension, generating G-quadruplex structures. These structures bind hemin to form DNAzymes that catalyze the localized production of reactive oxygen species (ROS), which subsequently etch the silver shell of the AuNS/Ag@4-ATP SERS nanoprobe. This etching causes the desorption of Raman reporters and a quantifiable 'signal-off' response. This biosensor achieves a remarkably low detection limit of 8.13 fM with a broad dynamic range from 10 fM to 150 nM, and exhibits high specificity, capable of discriminating single-nucleotide variants. Furthermore, it successfully quantified miR-204 in clinical cartilage samples, showing a strong correlation with real-time quantitative polymerase chain reaction results. The modular design of the O-HP also facilitated the adaptation of the platform for detecting miR-21, demonstrating its generalizability. This work provides a robust and versatile biosensing strategy with significant potential for clinical miRNA diagnostics.
研究领域:
生物传感器
·
分子诊断
·
纳米生物技术
·
微小RNA检测
·
即时检测
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DOI
高影响因子
Russian Journal of Physical Chemistry A
·
2022
·Vol. 96
·期 10
·2182-2188
·
IF 0.791
Khan Misbah Ullah,Ullah Hayat,Honey Shehla,Manan Abdul,Sarfaraz Maliha,Ullah Shaheed,Ullah Munzer,Gul Zarif
Abstract Efficient bimetallic nanostructures, which show properties related to two sole metals, have empowered inventive applications in nanosciences and nanotechnology. Here, we construct bimetallic Au@Ag nano-urchins with controlled Au tips for Catalysis and SERS application. The proposed nano-urchins were synthesized via electrochemical and simple galvanic replacement reaction route. The construction of Ag nano-flowers via electrochemical deposition use as a shell and Au tips were grown via galvanic reaction route greatly enhanced SERS properties of crystal violet (CV) detection and stability of nanostructures. In a comparison of Ag flower-like structures, the catalysis properties of Au@Ag nano-urchins are extremely efficient in degrading p -nitrophenol. As a result, these bimetallic nano-urchins show great development in SERS substrate and efficient catalysts.
研究领域:
纳米科学
·
材料科学
·
化学
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DOI
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2025
·
IF 5
Jun Dong,Yixuan Hou,Wei Gao,Chengyun Zhang,Lipeng Zhu,Yongkai Wang,Qingyan Han,Jianxia Qi
Surface-Enhanced Raman Scattering (SERS), with the exceptional single-molecule detection capability and high sensitivity, has become an advanced spectroscopic analysis method in fields such as chemical analysis and biosensing. Three-dimensional (3D) sandwich substrates, due to their unique structural characteristics, have attracted considerable attention for their ability to enhance SERS detection sensitivity. However, the controlled assembly of 3D sandwich substrates with bimetallic nanoparticles remains a challenge. In this paper, a fabrication method for constructing 3D sandwich substrates via a bottom-up approach is proposed. Using a liquid–liquid phase self-assembly method, Au@Ag nanocubes (Au@Ag NCs) were assembled on a polystyrene (PS) microsphere array with monolayer MXene (Ti 3 C 2 T X ) adsorption, successfully constructed an Au@Ag NCs/MXene/PS microsphere substrate. Furthermore, the SERS sensitivity of the substrate was evaluated using Rhodamine 6G (R6G), Crystal Violet (CV), and Aspartame (APM) molecules, and experimental results show that the detection limits are 10 −12 M, 10 −11 M, and 0.0313 g/L (1.06 × 10 −4 M), respectively, with excellent uniformity and stability of the substrate. These findings demonstrate that the constructed 3D substrate exhibits outstanding SERS performance and holds significant potential for applications in environmental pollutant detection and food safety.
研究领域:
分析化学
·
纳米生物技术
·
环境监测
·
食品安全
·
光谱学
·
材料科学
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DOI
高影响因子
Journal of Materials Chemistry C
·
2025
·
IF 5.1
Yuanjie Teng,Jie Li,Yantao Lou,Xingchen Zhang,Ze Wang,Naiwen Zhang,V. Balasubramani,Tianyu Hu,Zaifa Pan
Surface-enhanced Raman scattering (SERS) offers single-molecule sensitivity, but its practical use is constrained by the high cost and instability of noble-metal substrates. Semiconductor based SERS substrates are more stable and scalable, but typically suffer from weak charge transfer due to wide band gaps. Here we report a rare-earth doping strategy to boost the SERS activity of wide-band-gap ZnO. By introducing Er3+ with multielectron 4f orbitals into Zn1−xErxO (x = 0–8 mol%), the carrier density was simultaneously increased and new defect states were created that collaboratively enhance electromagnetic and chemical contributions. The optimized Er3+-doped ZnO substrate enables sensitive and reproducible detection of rhodamine 6G, achieving a detection limit of 2.4 × 10−8 M, an enhancement factor of 1.73 × 105, and excellent uniformity (RSD = 1.39%). Mott–Schottky analysis confirms carrier density increase upon Er doping, while DFT calculations attribute the dominant contribution to Er d-states and the introduction of 4f-induced surface defect levels that promote charge transfer. This work establishes rare-earth doping as an effective route to engineer semiconductor based SERS substrates and offers a mechanistic framework for designing non-metal SERS systems with synergistic electromagnetic and chemical enhancement.
研究领域:
材料科学
·
分析化学
·
纳米科技
·
半导体物理
·
光谱学
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DOI
高影响因子
危险品化学品经营许可证（带存储）