SENSORS
·
2019
·Vol. 19
·期 3
·674
·
IF 3.031
Hong Zhai,Yunfeng Bai,Jun Qin,Feng Feng
A novel colorimetric and ratiometric fluorescence sensor was constructed by using carbon quantum dots (CQDs) and o-diaminobenzene (ODB). Unlike ODB by itself, ODB oxide (oxODB) not only emits fluorescence, but also produces ultraviolet (UV) absorption. Therefore, on the basis of the potential optical properties of ODB, glucose oxidase (Gox) and horseradish peroxidase (HRP) were introduced into a CQDs–ODB system for the quantitative oxidation of ODB. When glucose is present, it is oxidized by oxygen under the catalytic action of its oxidase to form hydrogen peroxide. Hydrogen peroxide is a strong oxidant that can rapidly oxidize ODB through the catalysis of horseradish peroxidase. oxODB can cause changes in the fluorescence ratio (I550/I446) and absorbance ratio (A/A0). At the same time, the color of the detection solution can also change under sunlight and ultraviolet lamps. Therefore, glucose can be quantitatively detected by ratiometric fluorescence and colorimetry simultaneously, and semi-quantitatively detected by observing the colors with sunlight and ultraviolet lamps of 365 nm. This increases not only the convenience but also the accuracy of detection. In addition, this sensor has good selectivity and can be used for the determination of glucose in serum, providing a new idea for the development of blood glucose sensors.Keywords:dual-mode sensor;carbon quantum dots;o-diaminobenzene;glucose
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 3
·1-8
·
IF 5.479
Liu Shi Gang,Luo Dan,Han Lei,Li Nian Bing,Luo Hong Qun
A hybrid material composed of guanine-rich single stranded DNA (G-rich ssDNA) and cobalt oxyhydroxide (CoOOH) nanosheets is used as a nanoprobe for fluorometric turn-on detection of ascorbic acid (AA). The CoOOH nanosheets function as a recognition component for AA. The G-rich ssDNA is used to produce a G-quadruplex, and the G-quadruplex/thioflavin T (ThT) complex acts as a fluorescent reporter. In the absence of AA, p-phenylenediamine (PPD) is oxidized to form oxPPD which has a dark red color. It causes the fluorescence of the G-quadruplex/ThT complex to be quenched. However, in the presence of AA, the CoOOH nanosheets of the nanoprobe are preferentially reduced by AA. Hence, PPD is not oxidized, and fluorescence is not quenched. A fluorometric turn-on method was developed based on these findings. It has a detection limit of 94 nM and works in the concentration range from 1 to 10 and 20 to 80 μM. This method was applied to the determination of AA in (spiked) fruit juice samples.
Schematic presentation of a fluorescent assay of ascorbic acid (AA) is established using a nanoprobe composed of guanine-rich single stranded DNA (G-rich ssDNA) and cobalt oxyhydroxide (CoOOH) nanosheets. It is based on competitive reduction of CoOOH by p-phenylenediamine (PPD) and AA. Thioflavine T (ThT) induces the formation of fluorescent G-quadruplex/ThT complex. The oxidized form of PPD (oxPPD) can quench the fluorescence via fluorescence resonance energy transfer (FRET), but AA suppresses quenching.
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Food Analytical Methods
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2019
·Vol. 12
·期 5
·1086-1091
·
IF 2.413
Wu Zhengzong
Foodborne diseases arise from Listeria monocytogenes ( L. monocytogenes ) and Salmonella typhimurium ( S. typhimurium ) are global public health problems, influencing both developing and developed countries. Therefore, their rapid and accurate detection is of much concern in food safety and point-of-care diagnostics. In this study, a surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for simultaneous detection of them was developed. Under optimal conditions, the proposed assay showed good linear responses in the ranges of 10 2 –10 7 cfu/mL and 10 2 –10 7 cfu/mL with limits of detection of 75 cfu/mL and 75 cfu/mL, respectively, for L. monocytogenes and S. typhimurium , respectively. The practicality of the LFA in real food sample (milk) was also validated. Overall, the proposed assay is promising and has a good chance to be employed for multiple pathogen detection in food products.
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JOURNAL OF CELLULAR PHYSIOLOGY
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2019
·Vol. 235
·期 1
·465-479
·
IF 4.522
Ling-Hong Huang,Jin Han,Jian-Ming Ouyang,Bao-Song Gui
The interaction between nanohydroxyapatite (HAP) and smooth muscle cells is an important step in vascular calcification. However, the effect of the shape of HAP on adhesion and endocytosis to aortic smooth muscle cells has been rarely reported. Four different morphological HAP crystals (H-Rod, H-Needle, H-Sphere, and H-Plate) were selected to interact with rat aortic smooth muscle cells (A7R5). Fluorescence-labeled HAP was used to detect crystal adhesion and endocytosis and then pretreated with different endocytic inhibitors to explore the pathway of endocytotic crystals. The distribution of crystals inside and outside the cells and the crystal localization in lysosomes was observed through laser confocal microscopy. The effect of crystal on the cell cycle and the changes in the expression of phosphatidylserine, osteopontin, α-actin, core binding factor alpha 1, and osterix on the surface of A7R5 cells were detected. The adhesion and endocytosis of HAP on A7R5 cells were closely related to crystal shapes and ranked as follows: H-Plate > H-Sphere > H-Needle > H-Rod. H-Sphere and H-Needle were internalized into the cells mainly via the clathrin-mediated pathway, whereas H-Plate and H-Rod were internalized into the cells mainly via macropinocytosis. The endocytosed nano-HAP was mainly distributed in the cell lysosome. The adhesion and endocytosis of HAP to A7R5 cells were positively correlated with the specific surface area, and contact area of HAP and negatively correlated with the absolute value of Zeta and contact angle of HAP. This study provided insights into the effect of crystal morphology on vascular calcification and its mechanism.
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ANALYTICAL CHEMISTRY
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2019
·
IF 6.35
Zhiwei Qie,Qiqi Liu,Wenliang Yan,Zichen Gao,Wu Meng,Rui Xiao,Shengqi Wang
A universal and ultrasensitive immunochromatographic assay (ICA) was established using antigen as a bifunctional element and antialbumin antibody in a test line. Preincubation was introduced for competitive recognition. After optimization, the linear detection of aflatoxin M1 (AFM1) with quantum dot bead (QB)-based ICA (QB-ICA) sensor ranged from 10 to 52 pg mL–1, with a 50% inhibitory concentration (IC50) of 23 pg mL–1, which was nearly 49.6-fold lower than those of ICA on a traditional structure with traditional pretreatment (IC50 = 1.10 ng mL–1) and 10-fold lower than those of ICA on a traditional structure with acid aid pretreatment (IC50 = 0.25 ng mL–1). The limit of detection (LOD) for AFM1 was 16 pg mL–1 in milk, which was approximately 16.3-fold times higher than those of ICA on a traditional structure with traditional pretreatment and 6.3-fold higher than those of ICA on a traditional structure with acid aid pretreatment. The LOD improved by 20-fold by using the proposed structure compared to that of conventional enzyme-linked immunosorbent assay (ELISA) for AFM1-spiked milk samples (IC10 = 0.12 ng mL–1). The performance and practicability of the established QB-ICA sensor were validated with a commercial ELISA kit. To evaluate universality, we successfully detected chloramphenicol, with IC50 of 0.42 ng mL–1. Given its high sensitivity and universality, the proposed QB-ICA can be used as an alternative for rapid, sensitive, and universal quantitative detection of all small-molecule analytes.
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GREEN CHEMISTRY
·
2019
·Vol. 21
·期 14
·3930-3939
·
IF 9.405
Guo Qiu,Chongpin Huang,Xiuliang Sun,Biaohua Chen
Bio-based platform chemical 5-hydroxymethylfurfural (5-HMF) can be converted into large-scale, high value-added compounds generally obtained from petroleum. Hence, developing an efficient catalytic system to transform glucose (a cheap and abundant branch of biomass) into 5-HMF has drawn significant attention. Earlier reports have concentrated on a two-step conversion of glucose to 5-HMF involving glucose isomerization into fructose and subsequent dehydration. In this work, efficient heterogeneous niobium-loaded montmorillonite (Nb-MMT) catalysts containing Lewis and Brønsted acid sites have been synthesized by a readily cation-exchanged synthetic method and were used to convert glucose and showed high selectivity and conversion. An optimum 5-HMF yield of 70.52% with a 99% conversion of glucose was achieved in a biphasic solvent of methyl isobutyl ketone (MIBK)/water at 170 °C for 3 h. The catalytic system also exhibited an excellent activity in the conversion of disaccharides and polysaccharides to 5-HMF with satisfactory yields. In addition, Nb-MMT was effectively reused four times without significant loss of activity. Nb-MMT also featured good catalytic activity and selectivity towards carbohydrate conversion in water. Furthermore, the reaction mechanism for the transformation of glucose to 5-HMF over Nb-MMT was investigated by density functional theory (DFT) calculations, and the results showed that niobium oxyhydroxide species of Nb-MMT played an essential role in glucose conversion, and the catalytic mechanism was a synergetic proton transfer process via hydrogen bonding. This study paves the way for improvements in Nb-containing solid acid catalysts for conversion of carbohydrates.
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Toxins
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2019
·Vol. 11
·期 5
·289
·
IF 3.895
Yu Wu,Taotao Li,Liang Gong,Yong Wang,Yueming Jiang
Fusarium proliferatumcan infect many crops and then produce fumonisins that are very harmful to humans and animals. Previous study indicates that carbon sources play important roles in regulating the fumonisin biosynthesis. Unfortunately, there is limited information on the effects of carbon starvation in comparison with the carbon sources present in the host of fumonisin production inF. proliferatum. Our results indicated thatF. proliferatumcultivated in the Czapek’s broth (CB) medium in the absence of sucrose could greatly induce production of fumonisin, while an additional supplementation of sucrose to the culture medium significantly reduced the fumonisin production. Furthermore, cellulose and hemicellulose, and polysaccharide extracted from banana peel, which replaced sucrose as the carbon source, can reduce the production of fumonisin byF. proliferatum. Further work showed that these genes related to the synthesis of fumonisin, such asFUM1andFUM8, were significantly up-regulated in the culture medium in the absence of sucrose. Consistent with fumonisin production, the expressions ofFUMgene cluster andZFR1gene decreased after the addition of sucrose. Moreover, these genes were also significantly down-regulated in the presence of cellulose, hemicellulose or polysaccharide extracted from peel. Altogether, our results suggested that fumonisin production was regulated inF. proliferatumin response to different carbon source conditions, and this regulation might be mainly via the transcriptional level. Future work on these expressions of the fumonisin biosynthesis-related genes is needed to further clarify the response under different carbon conditions during the infection ofF. proliferatumon banana fruit hosts. The findings in this study will provide a new clue regarding the biological effect of the fumonisin production in response to environmental stress.Keywords:Fusarium proliferatum;fumonisin biosynthesis;carbon source;environmental stress;gene expressionKey Contribution:F. proliferatumcan produce fumonisins which are harmful to human health. This study aimed to explore the effect in the absence; followed by addition of carbon sources on fumonisin production; which will be beneficial for understanding the infection ofF. proliferatuminto banana fruit.
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ACS Omega
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2019
·
IF 2.584
Meng Li,Haiping Su,Yan Tu,Yazhuo Shang,Yu Liu,Changjun Peng,Honglai Liu
The alkylimidazolium tetrafluoroborate ionic liquids (ILs) ([Cnmim][BF4] n = 2, 4, 6, 8, 10) and anionic surfactant sodium dodecyl sulfate (SDS) were combined together to produce effective mediums for chromogenic catalysis of tetramethylbenzidine (TMB) with horseradish peroxidase (HRP) in the presence of H2O2. The chromogenic performance, kinetic behavior, and the possible influencing mechanism for the chromogenic catalysis of HRP-H2O2-TMB were discussed in detail. Therein, the roles of ionic liquids (ILs) were highlighted by the combination of experiments and theoretical calculations. The SDS/[C4mim][BF4] combination displayed superiority in chromogenic catalysis by improving both the substrate solubility and product stability to the maximum extent possible. Furthermore, SDS/[C4mim][BF4] combination showed uniqueness for TMB in improving the chromogenic performance compared with other chromogenic substrates of HRP. Inspired by the efficient chromogenic system, an enhanced enzyme-linked immunosorbent assay strategy for the detection of human immunoglobulin G was established and the sensitive colorimetric strategies for the detection of H2O2 and glucose were further developed by employing SDS/[C4mim][BF4] combination as the medium of chromogenic catalysis of HRP-H2O2-TMB. This unique chromogenic system is endowed with multitude of potential applications in biological systems.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
·
IF 2.931
Liqing Du,Zhongping Li,Jiaoli Yao,Guangming Wen,Chuan Dong,Hung-Wing Li
Silicon quantum dots have become one of the most popular nanomaterials in biological applications for their excellent biocompatibility and optical properties . Herein, we synthesized amino-functionalized silicon quantum dots (NH 2 @SiQDs) via a simple microemulsion method, in which silicon tetrachloride and allylamine were used as source of silicon and functional group. NH 2 @SiQDs exhibits good water-solubility, high fluorescence quantum yield and optical stability. A non-enzymatic biosensor of glucose was developed based on the fluorescence quenching of NH 2 @SiQDs in response to glucose. The fluorescence response was linearly proportional to glucose in the concentration range of 1.0 × 10 −6 –9.0 × 10 −5 mol/L and the detection limit was determined to be 3.0 × 10 −7 mol/L. The developed glucose sensor was successfully applied in blood glucose analysis of human serum. Satisfactory result that agreed very well with traditional method was obtained.
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ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Fusheng Zhang,Dongdong Wang,Haijuan Qin,Liang Feng,Xinmiao Liang,Guangyan Qing
Biological photonic nanostructures comprising a hierarchically self-assembled cellulose nanocrystal (CNC) have been exploited for the development of sensing, optoelectronics, and energy materials. Although multiple techniques are used for controlling the optical response and chiral nematic structure of CNC-derived materials, the presence of external studies that pristine CNC has chemoselectivity is not yet reported to implement this destination. Here, we report that the CNC film without modification shows a high optical sensitivity for glucose through color variation from blue to red. Moreover, various glucose homologs or analogs that only differ in terms of the orientation of a hydroxyl group are selectively distinguished through the naked eye. The excellent chemoselectivity of CNC is attributed to carbohydrate–carbohydrate selective hydrogen-bonding interactions. Close binding with glucose induces the rearrangement of a CNC chain and strengthens the repulsive interaction, thus increasing the helical pitch of the chiral nematic structure of the CNC film and changing its macroscopic color. This CNC chemoselectivity presents an unprecedented control of chiral nematic mesoporous carbon through monosaccharide species. The results provide a simple but highly efficient method to tune the optical and structural properties of CNC nanomaterials and to apply them for practical biosensors, chiral separation, and energy applications.
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JOURNAL OF CLUSTER SCIENCE
·
2019
·Vol. 31
·期 2
·337-346
·
IF 2.125
Zhao Chenyu,Jiao Junfeng,Zhou Wencui,Zhang Yong,Liu Han,Yang Xiaogang,Pandi Boomi,Cai Yurong
In this study, a new HAP/rGO/AuNPs-AO modified carbon electrode (GCE) was fabricated for amperometric detection of ascorbic acid. The HAP/rGO/AuNPs composite was prepared by one-step hydrothermal method. The large specific surface area, strong adsorption and excellent biocompatibility of HAP nanowires enabled fast, stable and accurate detection of ascorbic acid. As array of quantum dot, AuNPs had strong electrocatalytic activity in addition to good adsorption to enzymes, which could provide high sensitivity and low limit of detection for biosensor. Then rGO ensured that current signal could be quickly transferred from the composite to electrode. Under the optimum conditions, the prepared biosensor demonstrated a wide linear range from 0.39 to 36 mM (R 2 = 0.99845), a low limit of detection (3.39 μM, S/N = 3) and a high sensitivity (15.949 mA moL −1 ) with good selectivity, stability (10.8%) and repeatability (3.9%), which might providing a reasonable and reliable way for ascorbic acid detection.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-7
·
IF 5.479
Qiao Juan,Liu Qianrong,Wu Han,Cai Huiwu,Qi Li
A fluorescent probe is described for the determination of serum glucose after hepatotoxin-induced liver injury. The probe is based on the use of a water-soluble polymer and has been prepared from a multi-functional azlactone polymer as the linker, amino boronic acid, and Alizarin Red as the signalling moiety. The excitation/emission peaks of the polymeric fluorescent probe are at 468/567 nm. Fluorescence is reduced on addition of glucose. Intensity drops linearly in the 0.1 mM to 14 mM glucose concentration range. The probe was applied to non-enzymatic detection of glucose in rat serum after CCl 4 -induced liver damage.
A polymer based fluorescent probe has been constructed and applied for non-enzymatic monitoring of serum glucose following hepatotoxin induced liver injury.
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