Science Advances
·
2020
·Vol. 6
·期 6
·
IF 13.117
Zecong Xiao,Zhenwei Su,Shisong Han,Jinsheng Huang,Liteng Lin,Xintao Shuai
The response to programmed cell death protein-1 (PD-1)/programmed death ligand-1 (PD-L1) blockade in cancer immunotherapy is limited because of multiple immune evasion mechanisms. Here, a previously unknown strategy is proposed to synergize the nuclear factor κB (NF-κB) inhibition and PD-1 blockade for antitumor immunotherapy. A dual pH-sensitive nanocarrier loading curcumin (CUR) and anti–PD-1 monoclonal antibody (aPD-1) may bind to circulating PD-1+ T cells and then follow their infiltration into the tumor. Furthermore, the nanodrug bound to PD-1+ T cells may be released in the tumor microenvironment, leaving aPD-1 to block PD-1 on T cells and generating a CUR-encapsulated cationic nanodrug that can be easily taken up by tumor cells/tumor associated macrophages (TAMs). Thus, not only the antitumor T cells mediate efficient CUR delivery to tumor but also the efficient CUR delivery promotes the tumor infiltration of antitumor T cells, thereby resulting in effective activation of antitumor immunity.
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高影响因子
JOURNAL OF APPLIED TOXICOLOGY
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2020
·Vol. 40
·期 8
·1153-1161
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IF 2.997
Zhang Youyou,Yu Yalei,Zhang Jie,Guan Chuhuai,Liu Liang,Ren Liang
Early diagnosis of cantharidin-induced myocardial injury is the key to reduce the fatality rate in clinical practice. The purpose of the present study was to explore biomarkers that can be used for the prediction and diagnosis of cantharidin-induced myocardial injury. Of 65 male Sprague-Dawley rats weighing 200-230 g, 25 rats were divided into five groups according to the administration dose of cantharidin (0, 1.34, 2.67, 4 and 5.34 mg/kg; n = 5 per group) and the other 40 rats were treated with 2.67 mg/kg cantharidin and divided into nine groups according to the administration time (0, 1, 2, 4, 6, 8, 12, 24, 48 and 72 hours; n = 4 per group). Pathological changes of hypoxia, necrosis and inflammation were confirmed in heart samples that were exposed to cantharidin by hematoxylin-eosin staining and overall scores of pathological changes among heart samples in cantharidin exposure groups showed an increasing trend compared with in the control group. Coexpression of vascular endothelial growth factor (VEGF), hypoxia inducible factor-1α (HIF-1α) and caspase9 was shown in the myocardium by immunofluorescence staining. Western blotting results showed that expression of VEGF, HIF-1α and caspase9 in cantharidin-treated rat hearts showed an increasing trend compared with in the control group. Results of enzyme-linked immunosorbent assay suggested that plasma levels of troponin T (TN-T), VEGF and HIF-1α were elevated at different intervals after cantharidin administration, and VEGF and HIF-1α had a significant linear relationship with TN-T that was verified by multiple linear regression analysis. Preliminary results serve to illustrate that TN-T, VEGF and HIF-1α might be valuable molecular markers in cantharidin-induced myocardial injury and that diagnostic accuracy needs to be studied further.
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JOURNAL OF MOLECULAR LIQUIDS
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2020
·
IF 5.065
Xiaojie Liu,Chunying Zhu,Taotao Fu,Youguang Ma
The densities and viscosities of ternary solution of piperazine (PZ) + 2-Amino-2-methyl-1-propanol (AMP) + H 2 O were measured at P = 101 kPa and T = (303.15–343.15) K. The effects of temperature and concentration on the density and viscosity of ternary solution were investigated. The apparent molar volume ( V φ ), the limiting partial molar volume ( V φ 0 ), viscosity B -coefficient, Helper's constant and the free energies of activation per mole of solvent ( Δμ 1 0≠ ) and solute ( Δμ 2 0≠ ) were calculated from the density and viscosity, respectively. The solute-solute and solvent-solute interactions were analyzed from the volumetric and viscometric properties. The results show that the PZ has a destructive effect on the structure of mixed solvent.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2019
·
IF 2.298
Xing Cui,Hai Zhu,Chao-Hong He,Ke-Jun Wu
The thermal conductivities of 1-butyl-3-methylimidazolium l-tryptophan ([BMIM][l-trp]), water, ethanol, [BMIM][l-trp] + water, [BMIM][l-trp] + ethanol, water + ethanol, and [BMIM][l-trp] + water + ethanol were investigated from 283.15 to 333.15 K covering the whole scale of concentration at atmospheric pressure with the transient hot wire method. The thermal conductivities of pure [BMIM][l-trp] decease linearly from 0.180 to 0.177 W·m–1·K–1 with increasing temperature. The uncertainty of the measurement was less than 2% at the 0.95 confidence level. The experimental data of binary and ternary mixtures were correlated by a second-order Scheffé polynomial as a function of composition and temperature. The average relative deviation of the calculated values with experimental data was 0.58%.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Yahuan Wang,Chengyu Lin,Zhiwei Wang,Zhimin Chen,Jiafu Chen,Yong Chen,Shaohua Liu,Jianwei Fu
The development of novel adsorbents with high adsorption capacity and easy recovery property is imperative in the field of wastewater treatment . In this study, a hard template-induced assembly strategy was developed to fabricate the magnetic hollow poly(cyclotriphosphazene- co -4,4′-sulfonyldiphenol)-Fe 3 O 4 (PZS-Fe 3 O 4 ) hybrid nanocapsules , in which Fe 3 O 4 nanoparticles were well embedded in the cross-linked PZS shell. The resulting samples were well characterized using SEM, TEM , EDS , FT-IR, VSM , XPS , XRD and N 2 sorption . Then, using Safranine T (ST) as model dye, the adsorption behavior of as-prepared hollow PZS-Fe 3 O 4 nanocapsules including adsorption kinetics , adsorption isotherms , adsorption mechanism, and recyclability were systematically evaluated and discussed. The results revealed that the magnetic hollow PZS-Fe 3 O 4 nanocapsules own high adsorption capacity towards ST dye and outstanding magnetic separation functionality. The pseudo-second-order kinetic model and the Langmuir model can well describe the experimental data, and the adsorption process is controlled by more than one diffusion step. The interaction between ST dye and hollow PZS-Fe 3 O 4 nanocapsules is ascribed to π-π interaction and electrostatic interaction. The thermodynamic parameters demonstrated that the adsorption processes were physical, endothermic, and spontaneous. Additionally, the magnetic hollow PZS-Fe 3 O 4 nanocapsules also shows excellent peroxidase-like catalytic activity in the oxidation of 3,3′,5,5′-tetramethylbenzidine with H 2 O 2 , indirectly confirming the adsorption kinetic results.
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JOURNAL OF CHEMICAL THERMODYNAMICS
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2012
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IF 2.422
Yingjie Xu,Bin Chen,Wu Qian,Haoran Li
Values of the density and viscosity of the pure ionic liquid n -butylammonium nitrate (N4NO 3 ) and its binary mixtures with methanol, ethanol, 1-propanol, and 1-butanol were measured at temperature ranging from T = (293.15 to 313.15) K. The thermal expansion coefficient, molecular volume, standard entropy, and lattice energy of N4NO 3 were deduced from the experimental density results. The temperature dependence of the viscosity of N4NO 3 was fitted to the fluidity equation. Excess molar volumes V E and viscosity deviations Δ η for the binary mixtures were calculated and fitted to the Redlich–Kister equation with satisfactory results. Both excess molar volumes and viscosity deviations show negative deviations for (N4NO 3 + alcohol) mixtures. The effect of the temperature and the size of the alcohol on the excess molar volumes and viscosity deviations are discussed and analysed. Other derived properties, such as the apparent molar volume, partial molar volume, excess partial molar volume, thermal expansion coefficient, and excess thermal expansion coefficient of the above-mentioned systems were also calculated.
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MOLECULES
·
2015
·Vol. 21
·期 1
·27
·
IF 2.416
Xiya Zhang,Chao Wu,Kai Wen,Haiyang Jiang,Jianzhong Shen,Suxia Zhang,Zhanhui Wang
A new highly specific and sensitive monoclonal antibody (MAb) to T-2 toxin (T-2) was produced, providing an IC50value of 1.02 ng/mL and negligible cross-reactivity (CR) to other related mycotoxins. Based on the new MAb, a lateral-flow immunochromatographic assay (LFIA) using colloidal gold (CG) and fluorescent microspheres (FMs) as labels was proposed for T-2. Under the optimized conditions, in rapid qualitative assay, the cut-off values of the CG-LFIA were 400 μg/kg in rice and 50 μg/L in fresh milk, and the cut-off values of the FMs-LFIA were 100 μg/kg in both rice and chicken feed. For the quantitative assay with the FMs-LFIA, the limit of detection (LOD) were 0.23 μg/kg and 0.41 μg/kg in rice and chicken feed, respectively, and the average recoveries ranged from 80.2% to 100.8% with the coefficient of variation (CV) below 10.8%. In addition, we found that the CG-LFIA could tolerate the matrix effect of fresh milk better than the FMs-LFIA, while the FMs-LFIA could tolerate the matrix effect of chicken feed better than CG-LFIA under the same experimental conditions. These results provide a certain reference for the selection of appropriate labels to establish a rapid LFIA in various biological samples.Keywords:monoclonal antibody;colloidal gold;fluorescent microsphere;lateral-flow immuno-chromatographic assay;T-2 toxin
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POLYMER BULLETIN
·
2016
·Vol. 74
·期 4
·1091-1101
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IF 1.371
Li Hu,Zhou Zhen,Liu Jingcheng,Xu Wenjia,Liu Ren,Liu Xiaoya
Positive chemically amplified molecular glass with pendant t -butyloxy carbonyl ( t -BOC) groups based on β-cyclodextrin (β-CD) was prepared. The β-CD derivatives were obtained from the reaction of β-CD and di- tert -butyl dicarbonate (DBDC) in the presence of 4-dimethylaminopyridine, and the protecting ratio was controlled using various feed amounts of DBDC. Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance were employed to characterize the chemical structure, indicating that the synthesis was successful. Thermal behavior was characterized by thermogravimetric analysis; the results showed that the initial thermal decomposition temperature occurred at approximately 160 °C, which could satisfy the lithography process. Ultraviolet–visible spectrophotometer indicated that the t -BOC-protected β-CD derivatives had low absorbance at 365 nm wavelength. Additionally, X-ray diffraction analysis results showed that the β-CD derivatives had amorphous form, and exhibited excellent film-forming property. The photoresists consisting of different t -BOC protecting groups showed high sensitivity when exposed to 365 nm light and post-baked at 105 °C for 90 s, followed by developing in 2.38 wt% aqueous tetramethylammonium hydroxide solution at room temperature. An enhanced line and space positive patterns with 1 μm resolution were delineated according to digital microscope.
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ACS Nano
·
2024
·
IF 15.8
Bin Wang,Maoping Tang,Qijing Chen,William Ho,Yilong Teng,Xiaojian Xiong,Zhitong Jia,Xiuling Li,Xiaoyang Xu,Xue-Qing Zhang
T cell exhaustion has emerged as a major hurdle that impedes the clinical translation of stimulator of interferon genes (STING) agonists. It is crucial to explore innovative strategies to rejuvenate exhausted T cells and potentiate the antitumor efficacy. Here, we propose an approach utilizing MSA-2 as a STING agonist, along with nanoparticle-mediated delivery of mRNA encoding interleukin-12 (IL-12) to restore the function of T cells. We developed a lipid nanoparticle (DMT7-IL12 LNP) that encapsulated IL12 mRNA. Our findings convincingly demonstrated that the combination of MSA-2 and DMT7-IL12 LNP can effectively reverse the exhausted T cell phenotype, as evidenced by the enhanced secretion of cytokines, such as tumor necrosis factor alpha, interferon gamma, and Granzyme B, coupled with reduced levels of inhibitory molecules such as T cell immunoglobulin and mucin domain-3 and programmed cell death protein-1 on CD8+ T cells. Furthermore, this approach led to improved survival and tumor regression without causing any systemic toxicity in melanoma and lung metastasis models. These findings suggest that mRNA encoding IL-12 in conjunction with STING agonists has the potential to confer superior clinical outcomes, representing a promising advancement in cancer immunotherapy.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·Vol. 141
·期 31
·e55738
·
IF 2.7
Liangyong He,Yuqing Yang,Bin Yang,Weiqiang Hu,Zhengzhi Zheng,Tianyu Lu,Haijia He,Jiasheng Qian,Yaoyang Chen,Weiguo Zhang,Yuchao Ke
In recent years, optical diffusion film materials with high transmittance and high haze have been widely reported, while there is relatively rare research on their functionalization and practical application especially in real scenario. In this work, optical diffusion films using polymethyl methacrylate (PMMA)@TiO 2 @SiO 2 (P@T@S) composite microspheres were fabricated, which exhibited sufficiently high light transmittance and haze. The binding mode of PMMA microspheres and nanoparticles suggested that PMMA microspheres with a positive surface potential combined with nanoparticles with a negative surface potential through electrostatic attraction to achieve mutual binding. The prepared P@T@S composite microspheres showed better heat and swelling resistance than those of PMMA microspheres. The P@T@S diffusion film, whose excellent light diffusion effect was clearly demonstrated, exhibited the best optical performance among all optical diffusion films prepared. After passing through the P@T@S diffusion film, the laser light was not only uniform but also bright. The findings from this work can provide some insight into further research on optical diffusion films applied in optoelectronic device areas.
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ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Rihong Xiao,Jianping Yang,Yili Zhang,Zhuo Xiong,Junying Zhang,Yongchun Zhao
Industrial flue gas mercury emissions account for 30% of global atmospheric mercury emissions. While selenium-based materials show potential in reducing mercury emissions, the aspect of mercury recovery is neglected amidst the pressing need for mercury resources. Unlike conventional adsorbent regeneration cycles, elemental selenium anchoring mercury directly yields valuable by-products such as HgSe, a semiconductor with a narrow bandgap, considered ideal for optoelectronic devices. Amorphous selenium (α-Se) exhibits high mercury removal efficiency at room temperature but converts to less active trigonal selenium (t-Se) in high-temperature industrial flue gas. The literature on the mercury removal behavior of t-Se is limited. To address this issue, rod-shaped selenium (Se-rod) with specific exposed active crystal facets (101) and vacancy defects is synthesized, achieving an exceptional mercury adsorption capacity of 430.10 mg g −1 . Moreover, the optical performance of self-produced HgSe surpasses those of commercial samples. Se-rod can be utilized for mercury removal while also bridging the gap in mercury resource supply.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2012
·
IF 1.693
Xianjie Gong,Yongsheng Guo,Juan Xiao,Yuzhong Yang,Wenjun Fang
Densities, viscosities, and refractive indices are investigated for binary mixtures of 1,2,3,4-tetrahydronaphthalene with several n-alkanes, namely, octane, nonane, and decane, at different temperatures (293.15 K, 298.15 K, 303.15 K, and 313.15 K) and atmospheric pressure. The values of density (ρ) of the compounds were used to compute excess molar volume (VmE) of the solution. The refractive indices of these binary systems were also determined over the whole concentration range at 293.15 K and 303.15 K.
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