BIOCHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 3.8
Linsong Luo,Xiao Han,Xiaoyan Dong,Qinghong Shi,Yan Sun
The growth of biodiesel and oleochemical industries results in substantial surpluses of glycerol and consistently low prices. To absorb this surplus, we report a polymer-assisted in situ enzymes encapsulation strategy in which glycerol dehydrogenase (GDH) is encapsulated with polyvinylpyrrolidone (PVP) and cysteine (Cys) within ZIF-8 for glycerol conversion. The results showed that the encapsulated GDH/ZIF-8 composites presented stronger substrate affinities and higher catalytic efficiencies than free GDH as well as good stability. Among the free GDH and GDH/ZIF-8 composites, GDH/Cys/PVP@ZIF-8 presented the highest relative activity of 300.7 %. This could be attributed to the structural evolution of the encapsulated GDH and the formation of a more hydrophilic microenvironment around GDH during complexation with PVP. To increase cofactor utilization, a cofactor self-sufficient cascade system was constructed by encapsulating GDH and forming H 2 O NADH oxidase (Nox) with PVP and Cys within ZIF-8 for the conversion of glycerol to 1,3-dihydroxyacetone (DHA). At the optimal GDH:Nox molar ratio (4:1) and a substrate concentration of 100 mM, the DHA yield reached approximately 4.2 mM in GDH-Nox/Cys/PVP@ZIF-8, and the total turnover number of GDH-Nox/Cys/PVP@ZIF-8 reached 2787 at 0.05 µM NAD + , indicating its feasibility for large-scale DHA production. This work has thus provided a promising procedure of co-immobilizing enzymes via Cys/PVP-assisted in situ encapsulation within ZIF-8 for the development of economically viable multienzyme biocatalysts for glycerol conversion.
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TETRAHEDRON-ASYMMETRY
·
2014
·
IF 2.165
Rui-Jie Chen,Gao-Wei Zheng,Yan Ni,Bu-Bing Zeng,Jian-He Xu
A new NADH-dependent carbonyl reductase Rh CR capable of efficiently reducing the ε-ketoester ethyl 8-chloro-6-oxooctanoate (ECOO) to give ethyl ( S )-8-chloro-6-hydroxyoctanoate [( S )-ECHO], an important chiral precursor for the synthesis of ( R )-α-lipoic acid, was identified from Rhodococcus sp. ECU1014. Using recombinant Escherichia coli cells expressing Rh CR and glucose dehydrogenase used for the regeneration of cofactor, 440 g L −1 (2 M) of ECOO were stoichiometrically converted to ( S )-ECHO in a space–time yield of 1580 g L −1 d −1 without the external addition of any expensive cofactor.
研究领域:
生物催化
·
酶工程
·
合成化学
·
生物技术
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JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2022
·
IF 3.262
Lingzhi Zhang,Yinghao Wang,Fang Zheng,Di Zhu,Yongmei Liang,Quan Shi
The collision cross-section (CCS) values of ions determined by ion mobility-mass spectrometry (IM-MS) can be used to deduce the shape and size of the ions. For each compound, as well as its isomer or tautomer, a unique arrival time peak was obtained in extracted ion mobility (EIM) spectra, which corresponded to a specific CCS value. However, the generation of solvated ions by electrospray ionization (ESI) increases the number of mobility peaks, which makes the EIM spectra difficult to interpret. In this study, solvent clusters formed by acetonitrile and methanol around 1,8-naphthalic anhydride (1,8-NA) cations ([C12H6O3 + H]+1,8-NA) were investigated using trapped ion mobility spectrometry-time-of-flight mass spectrometry (TIMS-TOF MS). The effects of infusion flow rate, nebulizer gas pressure, drying gas rate, and drying gas temperature on the formation of solvent clusters from acetonitrile and methanolic solution were systematically studied. The formation of solvent clusters was observed with infusion flow rates increased, which was manifested by the larger experimental CCS values of [C12H6O3 + H]+1,8-NA. Acetonitrile tended to form solvent clusters around ions more readily than methanol. These solvent clusters were stable enough to be detected by TIMS, but they cannot survive under ion activation conditions of mass spectrometry (MS). Increasing the nebulizer gas pressure seems to be a better way to eliminate the formation of solvent clusters in TIMS-TOF MS and give a “cleaner” EIM spectra. The current research demonstrates that more attention should be paid to the solvent effect on CCS values and their interpretation.
研究领域:
分析化学
·
质谱学
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DOI
PubMed
高影响因子
Micro & Nano Letters
·
2020
·Vol. 15
·期 7
·469-473
·
IF 0.975
Meiling Yang,Mingwang Liu,Zipeng Wu,Yu He,Yili Ge,Gongwu Song,Jiangang Zhou
A facile method for fabricating polymer carbon dots (PCDs) with characteristic dual-emission at 570 and 620 nm by 1,8-naphthalenediol in n-propanol solvents was designed. The synthesised PCDs possessed fluorescence enhancement performance toward the water in organic solvents. Based on this peculiar phenomenon, a highly efficient fluorescent probe for water detection in organic solvents such as N,N-dimethylformamide, dimethyl sulfoxide and n-propanol was established with a wide detection range from 0 to 50%. The probe can also be utilised to determine water content in other organic solvents. The practical application was explored by studying the response of PCDs toward the water in various spirit samples. This method may offer a new way of detecting water in organic solvents by PCDs.
研究领域:
材料科学
·
分析化学
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PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2025
·
IF 1.9
Yang Li,Wei Zhao,Qifeng Li,Jiayu Zhang,Anquan Yang,Min Xie,Ranhua Xiong,Chaobo Huang
Erigeron breviscapus, a medicinal plant rich in flavonoids with diverse pharmacological properties is an important resource. To maximize the utilization of these valuable bioactive compounds, ultrasound-assisted extraction combined with response surface methodology was used to optimize the extraction conditions for total flavonoids. After comparing six macroporous resins’ adsorption and desorption characteristics, AB-8 was selected for enriching total flavonoids, and its adsorption kinetics and thermodynamics were investigated. The following parameters were chosen as optimal for the dynamic adsorption/desorption experiments using an AB-8 filled column: sample loading concentration of 1.14 mg/mL, sample loading flow rate of 2 BV/h, elution solvent of 70% ethanol, desorption flow rate of 1 BV/h, elution solvent volume of 5.5 BV, pH of 2, temperature of 25 °C. After enrichment, the concentration of flavonoids was 5.16 times higher than that of crude extract. LC-MS identified 54 flavonoid molecules with excellent antioxidant properties.
研究领域:
植物化学
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药学
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分析化学
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PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2022
·
IF 10.53
Yinghui Xu,Xuan Zhao,Ran Chang,Hongqiang Qu,Jianzhong Xu,Jing Ma
Rationally regulating the separation pores is an effective way to achieve efficient separation membrane . However, the developed metal-organic framework (MOF) membranes for water treatment are all formed by one kind of MOF, which can only provide rigid pores, makes it difficult to achieve high flux and excellent rejection ratio simultaneity. Here, we proposed a facile strategy for the synthesis of heterogeneous MOF-on-MOF membrane with hierarchical pores, by site-specific grown ZIF-8 layer on UiO-66-NH 2 layer. Thus unique architecture of the obtained double-layered MOF membrane (UiO-66-NH 2 @ZIF-8) endows multidimensional separation pores: (i) the large pores of UiO-66-NH 2 can facilitate water transportation, (ii) the molecular-sieving pores of ZIF-8 can intercept dyes, (iii) UiO-66-NH 2 acting as active sites can promote the growth of ZIF-8. As a result, the optimized UiO-66-NH 2 @ZIF-8 membrane exhibits high flux (36.7 L m −2 h −1 bar −1 ) and excellent dye rejections (>97.0%). Through analysis and comparison, the regulated multi-type pores play an important role in membrane performance. Coupled with the facile membrane preparing method, this work can used as a potential approach of designing other advanced MOF membrane for water treatment.
研究领域:
材料科学
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环境工程
·
化学工程
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Annals of Translational Medicine
·
2022
·Vol. 10
·期 18
·971-971
·
IF 3.616
Shuqi Zhao,Meifeng Chen,Zhu Yu,Thi Thai Hoa Pham,Shutian Mo,Yongfei He,Tianyi Liang,Wenlong Cao,Chuangye Han
BackgroundBreast cancer (BC) is the most common type of cancer affecting females. It is also a leading cause of cancer-related death in women worldwide.MethodsSonodynamic therapy (SDT) is an emerging therapeutic strategy for cancer treatment. SDT ensures non-invasive penetration of deep tumors and results in activation of non-toxic sonosensitizers administered in deep tumor sites to become cytotoxic. It has been reported that 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD) has a significant anti-tumor effect against various cancer types including BC. However, DMDD is hydrophobic. Therefore, a one-step encapsulation method was used in the current study to construct zeolitic imidazole frameworks-8 (ZIF-8) loaded with DMDD and sonosensitizer chlorin e6 (Ce6). ZIF-8 was further modified by coating it with a biomimetic cell membrane to improve targeted delivery.ResultsIn vitro and in vivo results indicated that the nanomedicines had great biocompatibility properties and targeting ability. The nanocomposite exhibited a higher release rate under an acidic tumor microenvironment. The tumor killing effect of reactive oxygen species (ROS) generated from Ce6 and inhibition of tumor growth was enhanced after ultrasound (US) treatment, which might be caused by the increase in apoptosis rate.ConclusionsThese findings show that the combination of nanomedicine and SDT provides a potential therapeutic method for BC.Keywords: Breast cancer (BC), 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD), metal-organic framework, sonodynamic therapy, ZIF-8 nanoparticles
研究领域:
肿瘤学
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纳米技术
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药学
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治疗学
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DOI
PubMed
高影响因子
Lipids in Health and Disease
·
2018
·Vol. 17
·期 1
·1-7
·
IF 2.663
Luo Mengdie,Su Xin,Yi Yuhong,Yang Yang,Peng Daoquan
Background Angiopoietin-like protein 8(ANGPTL8) and apolipoprotein CIII (apoCIII) were found to inhibit the activity of lipoprotein lipase (LPL) and disrupt the clearance of triglyceride-rich lipoproteins (TRLs), leading to hypertriglyceridemia. Whether any relationship exists between these two important modulators of triglyceride metabolism has not been reported. Besides, whether ANGPTL8 concentration is altered in the patients with coronary artery disease (CAD) is still unclear. Methods A hospital-based case-control study was conducted. Sixty-eight CAD subjects and fifty-two nonCAD controls were recruited. Plasma apoCIII, ANGPTL8 was measured. Results ANGPTL8 and apoCIII concentration exhibited no significant difference between CAD group and nonCAD group. Both ANGPTL8 and apoCIII were significantly correlated with triglyceride level( r = − 0.243, P = 0.008; r = 0.335, P < 0.001, respectively). Regression analysis revealed that apoCIII was an independent contributor to triglyceride level independent of ANGPTL8 concentration (standardized β = 0.230, P < 0.01). Conclusion ApoCIII may mediate the effects of ANGPTL8 on triglyceride metabolism.
研究领域:
医学
·
分子生物学
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PubMed
高影响因子
Materials Today Chemistry
·
2026
·
IF 7.4
Mingjun Cui,Shijun Huang,Jiate Xue,Xinyu Chen,Siming Ren
2D nanocontainers are prepared with barrier, corrosion inhibition and monitoring. • BZ-P/EP coating shows higher .|Z| f = 0.01 Hz of 4.1 × 10 10 Ω cm 2 after 60 days immersion. • PH-responsive ZIF-8 enables the rapid release of Phen under acidic conditions. • Visual corrosion warning is achieved via Fe 2+ –Phen coordination. To overcome the limitations of conventional anticorrosion coatings, which typically exhibit single functionality and lack early-stage corrosion warning capability, this study presents the design and fabrication of a two-dimensional intelligent nanocontainer system. Specifically, a pH-responsive zeolitic imidazolate framework (ZIF-8) was grown in situ on hexagonal boron nitride (h-BN) nanosheets, and subsequently loaded with 1,10-phenanthroline (Phen), enabling the integration of physical barrier protection, active corrosion inhibition, and visual corrosion monitoring. UV–vis absorption spectra and electrochemical impedance spectroscopy (EIS) results demonstrate that the BZ-P nanocontainers not only provide effective corrosion inhibition but also exhibit rapid, pH-triggered release of Phen in mildly acidic conditions. After incorporation into an epoxy matrix, the resulting BZ-P/EP composite coating exhibits a high low-frequency impedance modulus (|Z| f = 0.01 Hz ) of 4.1 × 10 10 Ω cm 2 after 60 days of immersion in 3.5 wt% NaCl solution, which is approximately 2270 times higher than that of the neat epoxy coating. This improvement is accompanied by a significant reduction in oxygen content on the underlying steel surface (from 19.56 wt% to 0.62 wt%), indicating markedly suppressed corrosion. Under damaged coating conditions, the BZ-P/EP system maintains effective corrosion protection, as evidenced by a relatively high impedance modulus and low local admittance. This enhanced performance can be attributed to the synergistic effects of BN and ZIF-8, which combines structural barrier reinforcement with active corrosion inhibition. Furthermore, the released Phen molecules at damaged sites coordinate with Fe 2+ ions to form a red complex, enabling rapid and intuitive visual detection of corrosion initiation. This work provides a novel strategy for developing intelligent coating systems that integrate corrosion protection, active corrosion inhibition, and real-time monitoring, offering significant potential for applications in harsh service environments.
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Precision Chemistry
·
2026
·
IF 7.7
Liu Jibiao,Xia Hongjin,Zhang Zheng,Yu Zhixuan,Hu Yidong,Wu Zexian,Zhang Qiang,Li Qingyang
A zeolitic
imidazolate framework-8 film is controllably synthesized
on various substrates in 5 min through integrating zinc electrodeposition
and microwave-solvothermal treatment first, representing the cheapest
and fastest route until now. This rapid and cheap strategy opens up
a new avenue for the rational design and synthesis of metal–organic
framework films.
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JOURNAL OF FLUORESCENCE
·
2026
·
IF 3.4
Huang Zewei,Wang Yingjie,Ye Meiying,Cheng Heyong
Real-time, online and accurate detection of ferric/ferrous ions is quite important for environmental protection and healthcare, which is preferred by portable fluorescent sensors (in solid state) compared to previous sensors (dominant in liquid or turbid suspensions) associated with susceptibility to oxidation and precipitation. Considering the scarcity of the solid-state fluorescent sensors, a nitrogen-doped carbon quantum dots (NCQDs) encapsulated ZIF-8 (NCQD@ZIF-8) photonic crystal (PC) sensor for Fe 3+ and ascorbic acid detection in solid state was constructed by rapid evaporation self-assembly technique. The fluorescence intensity of NCQD was enhanced with slow light effect of ZIF-8 PC. Fe³⁺ was selectively detected based on its ability to quench the fluorescence of QDs@ZIF-8 PC through a combination of the internal filter effect and possible Fe 3+ −QDs complexation. Detection of ascorbic acid (AA) was based on the fact that AA can recover the fluorescence intensity of the NCQD@ZIF-8 PC quenched by Fe 3+ due to AA reduction of Fe 3+ to Fe 2+ . Both Fe 3+ and ascorbic acid can be quickly and selectively detected by the NCQD@ZIF-8 PC sensor in the linear range of 0. 1− 10 mmol L − 1 (R 2 = 0.996) with detection limits of 0.06 mmol·L − 1 and 100 µL sample consumption. Iron speciation analysis (Fe 3+ and Fe 2+ ) in simulated water samples was realized with good precision and accuracy. The NCQD@ZIF-8 PC sensor provided a rapid and portable way for determination of Fe 2+ /Fe 3+ in environmental samples and new possibilities for device fabrication. Graphical
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2026
·
IF 2.8
Haowei Bai,Yingbo Chen,DengChang Zhu,Yongxiang Shi
To address the limitation of traditional minoxidil sulfate (MXS) nano-drug delivery systems—namely, short dosing cycles, frequent replacement requirements, and inability to fulfill cosmetic coverage needs simultaneously—this study aims to develop a composite fiber with dual functions: sustained release and cosmetic coverage. Employing a green and simple wet spinning technique, MXS-loaded ZIF-8 nanoparticles were functionally composite with a polyvinyl alcohol (PVA) polymer matrix, successfully yielding PVA/ZIF-8 composite fibers. The resulting composite fiber exhibits excellent mechanical properties, with a tensile strength reaching 2.5 cN/dtex and good aging resistance. The fiber inherits the high drug-loading capacity of ZIF-8 nanoparticles, achieving sustained release for up to 220 h. Additionally, the composite fiber possesses outstanding processability, enabling its further weaving into wigs. This study successfully developed a novel PVA/ZIF-8 composite fiber integrating sustained drug release with physical hair coverage. It expands new application directions for functional polymer composites in the biomedical field.
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