JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Meng-Meng Du,Zhan-Tao Zhu,Ge-Ge Zhang,Yun-Qiu Zhao,Bei Gao,Xin-Yi Tao,Min Liu,Yu-Hong Ren,Feng-Qing Wang,Dong-Zhi Wei
The study aims to enhance β-amyrin production in Saccharomyces cerevisiae by peroxisome compartmentalization. First, overaccumulated squalene was determined as a key limiting factor for the production of β-amyrin since it could inhibit the activity of β-amyrin synthase GgbAs1. Second, to mitigate the inhibition effect, the enhanced squalene synthesis pathway was compartmentalized into peroxisomes to insulate overaccumulated squalene from GgbAs1, and thus the specific titer of β-amyrin reached 57.8 mg/g dry cell weight (DCW), which was 2.6-fold higher than that of the cytosol engineering strain. Third, by combining peroxisome compartmentalization with the “push-pull-restrain” strategy (ERG1 and GgbAs1 overexpression and ERG7 weakening), the production of β-amyrin was further increased to 81.0 mg/g DCW (347.0 mg/L). Finally, through fed-batch fermentation in a 5 L fermenter, the titer of β-amyrin reached 2.6 g/L, which is the highest reported to date. The study provides a new perspective to engineering yeasts as a platform for triterpene production.
研究领域:
生物技术
·
代谢工程
·
合成生物学
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 7
·3917-3930
·
IF 6.317
Yan Qiao,Xianfeng Ye,Lingli Zhong,Chengyao Xia,Lei Zhang,Fan Yang,Yongkai Li,Xiaodong Fang,Lei Fu,Yan Huang,Hui Cao,Zhoukun Li,Zhongli Cui
The β-glucan from Saccharomyces cerevisiae is a potent adjuvant that exhibits a broad spectrum of biological activities and health benefits, and different processes have been established to prepare active β-glucan from yeast. However, studies concerning the effect of β-1,6-glucanase enzymolysis on the structure and immunomodulatory activity of yeast β-1,3-glucan are scarce. In this study, we aim to develop a novel enzymatic process for the preparation of immunologically active β-glucan (BYG) from baker's yeast using a β-1,6-glucanase GluM. The β-1,6-glucan in fungal cell wall was specifically hydrolyzed by GluM, and resulted in cell wall decomposition and β-glucan release. Batch production of BYG was realized with 17.8% yield, 85.3% purity and 75.4% recovery rate. Structural characterization indicated that BYG exhibits rod-like structures with natural triplex and nanoparticle-like substructures compared with the commercial Glucan 300. BYG ameliorated inflammation in a DSS-induced mouse model of colitis through inhibiting oxidative stress (NO, MDA and MPO), inflammatory mediators (NLRP3, ASC, caspase-1, iNOS and COX-2), and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, IFN-γ), increasing the expression levels of tight junction proteins (ZO-1, occludin and claudin-1) and modulating the production of gut microbiota-synthesized SCFAs compared to the control. Our results showed that yeast β-1,3-glucan prepared with β-1,6-glucanase exhibits structural integrity that is responsible for its favorable immunomodulatory activity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 104
·期 11
·4927-4943
·
IF 3.53
Deng Pengjun,Meng Chunyu,Wu Yan,Xu Jie,Tang Xiaoqing,Zhang Xuecheng,Xiao Yazhong,Wang Xiaotang,Fang Zemin,Fang Wei
A novel β -glucosidase, BglD1 with high β -galactosidase and transglycosidation activities, was screened and cloned from the deep-sea bacterium Bacillus sp. D1. BglD1 exhibited the maximal β -glucosidase and β -galactosidase activities at 55–60 °C and pH 5.5–6.0. The enzyme maintained approximately 50% of its original activity at 35 °C and pH 6.0 after 120-h incubation. When applied to synthesize galacto-oligosaccharides (GOS), BglD1 generated 118.3 g/L GOS (33.8% (w/w)) from 350 g/L lactose, with trisaccharide Gal- β (1 → 3)-Lac and disaccharide Gal- β (1 → 4)-Gal as the main components. Furthermore, BglD1 could hydrolyze lactose in milk and produce GOS simultaneously. Using milk as the substrate, BglD1 hydrolyzed 88.5% lactose and produced 3.3 g/L GOS after incubation at 30 °C for 1 h. To improve the transglycosidation activity, a mutant BglD1:E224T was generated based on the semi-rational design. The GOS yield of BglD1:E224T was 11.5% higher than that of BglD1 when using lactose solution as the substrate. Thus, BglD1 and the mutant could be used as beneficial alternatives of the existing β -galactosidases for the production of GOS.
✓ 已验证
DOI
PubMed
高影响因子
ACS Synthetic Biology
·
2023
·
IF 4.7
Surui Lu,Hong Deng,Chenyao Zhou,Zhengda Du,Xuena Guo,Yanfei Cheng,Xiuping He
β-Caryophyllene is a plant-derived bicyclic sesquiterpene with multiple biological functions. β-Caryophyllene production by engineered Saccharomyces cerevisiae represents a promising technological route. However, the low catalytic activity of β-caryophyllene synthase (CPS) is one of the main restrictive factors for β-caryophyllene production. Here, directed evolution of the Artemisia annua CPS was performed, and variants of CPS enhancing the β-caryophyllene biosynthesis in S. cerevisiae were obtained, in which an E353D mutant enzyme presented large improvements in Vmax and Kcat. The Kcat/Km of the E353D mutant was 35.5% higher than that of wild-type CPS. Moreover, the E353D variant exhibited higher catalytic activity in much wider pH and temperature ranges. Thus, both the higher catalytic activity and the robustness of the E353D variant contribute to the 73.3% increase in β-caryophyllene production. Furthermore, the S. cerevisiae chassis was engineered by overexpressing genes related to β-alanine metabolism and MVA pathway to enhance the synthesis of the precursor, and ATP-binding cassette transporter gene variant STE6T1025N to improve the transmembrane transport of β-caryophyllene. The combined engineering of CPS and chassis resulted in 70.45 mg/L of β-caryophyllene after 48 h of cultivation in a test tube, which was 2.93-fold of that of the original strain. Finally, a β-caryophyllene yield of 594.05 mg/L was obtained by fed-batch fermentation, indicating the potential of β-caryophyllene production by yeast.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2014
·Vol. 28
·期 8
·635-640
·
IF 2.017
Xiaohua Zhao,Xiang Liu,Ming Lu
Low-melting β-cyclodextrin/ N -methylurea (NMU) mixture, an efficient catalytic system for ligand-free Suzuki and Heck couplings in the presence of fresh native β-CD-capped Pd 0 nanoparticles, has been successfully reported. This natural and convenient system can be performed in air and could afford the corresponding cross-coupled products in good to excellent isolated yields after a simple workup under every low Pd loading (0.05 mol%). Remarkably, the catalytic system can be recycled and reused without loss of catalytic activity. Copyright © 2014 John Wiley & Sons, Ltd.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Yulin Fan,Fang Fu,Langqiu Chen,Jiping Li,Jing Zhang,Guochao Zhang,Jingyi Liao
Based on reconstructing conventional alkyl β - d -glucopyranosides, alkyltri(oxyethyl) β - d -glucopyranosides ( 4a ~ 4h , n = 6 ~ 16) were prepared by introducing a tri(oxyethyl) group ((–OCH 2 CH 2 –) 3 ) as a hydrophilic spacer to improve effectively the related water solubility. Their foaming performance, emulsifying property, hygroscopicity, solubility, and thermotropic phase transitions were further investigated. The experimental results showed that the phase transition temperature (T iso ) increased with increasing their alkyl chain length. Such β - d -glucopyranosides ( 4b ~ 4h , n = 7 ~ 16) showed a smectic focal conic-shaped texture. Their water solubility, surface tension, CMC value, and hygroscopicity decreased with increasing their alkyl chain length. Meanwhile, 4e (n = 10) had the best foamability. In the rapeseed oil/water system, 4f (n = 12) had excellent emulsifying property. Furthermore, 4 (n = 8, 10, 12) and SDS or CTAC had the synergistic effect because their experimental CMC value was smaller than their ideal CMC value (CMC id ).
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Parbeen Singh,Xiaohong Ren,Tao Guo,Li Wu,Shailendra Shakya,Yaping He,Caifen Wang,Abi Maharjan,Vikramjeet Singh,Jiwen Zhang
Cyclodextrins nanosponges (CD-NSPs) are highly microporous crosslinked polymers with potential applications in the delivery of small and macro-molecular therapeutic agents. Despite the potent host-guest inclusion property, their inherent lack of cellular binding ability has limited applications in drug delivery. Herein, we functionalized the surface of β-cyclodextrin nanosponge (β-CD-NSP) with cholesterol, which is endogenous physiological molecules, widely distributed in all cells, and responsible for cell interactions and protein binding. The surface grafting of synthesized β-CD-NSP was confirmed with spectroscopic, microscopic, thermogravimetric, and chromatographic techniques. Moreover, β-CD-NSP was found to be safe in cytotoxicity assay. Doxorubicin (Dox) was selected as a model drug for drug adsorption study of cholesterol hydrogen succinate (CHS) grafted β-CD-NSP. The cellular uptake of NSP was found to be enhanced after CHS modification confirmed by confocal laser scanning microscopy (CLSM). Thus, proposed CHS modified β-CD-NSP system could be used as a site-specific drug delivery carrier.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
·
2026
·
IF 21.8
Xie Fang,Liang Wenwen,Liu Xin,Wang Yuehui,He Zhijun,Cai Jie
Despite the promising potential of emulsions as oral delivery vehicles, challenges such as insufficient interfacial stability, poor encapsulation efficiency, and limited mucosal adhesion still hinder their effectiveness in delivering lipophilic bioactives. In this study, we developed a high internal phase Pickering emulsion (HIPPE) system stabilized by zein–tannic acid–β-glucan (ZTB) ternary complex particles to enhance the oral delivery of β-carotene. The incorporation of β-glucan markedly improved the droplet size uniformity, interfacial protein adsorption, and storage and digestion stability of HIPPEs. The optimized ZTB − 0.6 -stabilized HIPPEs achieved a high encapsulation efficiency of ~ 88.77%, β-carotene retention of ~85.57% after 28 days of storage at 4 °C, and bioaccessibility of ~ 24.10% during in vitro digestion. Rheological tests confirmed desirable shear-thinning and thixotropic properties, suitable for 3D food printing. Furthermore, in vivo evaluations using a DSS-induced murine colitis model revealed that β-carotene-loaded ZTB-HIPPEs exerted multifaceted therapeutic effects: they effectively mitigated colonic inflammatory responses, bolstered the endogenous antioxidant capacity, and contributed to the restoration of intestinal barrier integrity. These findings highlight the potential of ZTB-stabilized HIPPEs as a multifunctional platform for targeted nutrient delivery and intestinal health improvement. Graphical
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Siyi Du,Haozhe Xu,Mengdan Yang,Ning Pan,Tiefeng Zheng,Chenyi Xu,Yan Li,Zhaojiang Zuo
Volatile organic compounds (VOCs) promote cyanobacteria dominating eutrophicated waters, with aquatic plant decrease and even disappearance. To uncover the toxic mechanism of cyanobacterial VOCs on aquatic plants, we investigated the growth, photosynthetic pigment levels, photosynthetic abilities and related gene expression in duckweed treated with β-cyclocitral and β-ionone, 2 main components in the VOCs. The levels of chlorophylls and carotenoids gradually declined with raising the concentration of the 2 compounds and prolonging the treatment time. Their decline should result from the down-regulation of 8 genes associated with photosynthetic pigment biosynthesis and up-regulation of 2 genes involved in carotenoid degradation. The reduction was also found in the photosystem II (PSII) efficiency and O 2 evolution rate, which should result from the lowered photosynthetic pigment levels and down-regulation of 38 genes related with photosynthetic process. The frond numbers, total frond area and fresh weight gradually decreased with raising the 2 compound concentration, which may result from the lowered photosynthetic abilities as well as down-regulated expression of 7 genes associated with growth-promoting hormone biosynthesis and signal transduction. It can be speculated that cyanobacterial VOCs may poison aquatic plants by lowering the photosynthesis and growth through altering related gene expression.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CRYSTENGCOMM
·
2021
·Vol. 23
·期 21
·3892-3900
·
IF 3.545
Dandan Ju,Xiaoli Gao,Shouchao Zhang,Yang Li,Wenjing Cui,Yinhui Yang,Mingyan Luo,Shujing Liu
Temperature is one of the physical parameters that affects the emission luminescence properties of upconversion (UC) materials. Temperature-dependent emission color tuning and good temperature sensitivity in multifunctional β-NaYF4:Yb/Er@β-NaYF4:Yb/Tm microcrystals were achieved under 980 nm excitation. Based on the different emission bands (blue, green and red bands) and spectral sensitivities of the core–shell structured microcrystals to temperature, intense white-light emission was obtained at room temperature and a real-time naked-eye recognizable emission color change from white to the purplish-pink region was realized with the temperature increasing from 300 to 500 K. Furthermore, optical temperature sensing properties of the sample with good sensitivity (from 0.0034 to 0.0044 K−1) were investigated by using a fluorescence intensity ratio between 521 nm (2H11/2 → 4I15/2 transition) and 540 nm (4S3/2 → 4I15/2 transition) in the temperature range from 300 to 500 K. The results indicate that the sample has high sensitivity in a relatively wide range of temperature intervals, especially in the low temperature region. We believe that the multifunctional core–shell structured microcrystals exhibit promising applications in white-light emitting diodes, the anti-counterfeiting field, and temperature sensing.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 49
·34884-34890
·
IF 3.9
Quanfang Wang,Yuefeng Sun,Shidi Zhao,Chuqi Bai,Zhiwei Cong,Yalin Dong,Taotao Wang
Residual antibiotics in nature are an important cause of antimicrobial drug resistance, and how to deal with residual β-lactam antibiotics in aqueous environments has become an urgent issue. In this work, magnetic zeolitic imidazolate frameworks-8 (ZIF-8) for immobilizing metallo-β-lactamases (MBLs), or Fe3O4@ZIF-8@MBLs, were successfully synthesized using the one-pot method in aqueous solution. The morphology and chemical structure of Fe3O4@ZIF-8@MBLs were characterized by scanning electron microscopy, energy dispersive spectra, X-ray diffraction, infrared spectra, physical adsorption, and zeta potential. Further, the degradation performance of Fe3O4@ZIF-8@MBLs for β-lactam antibiotics (penicillin G, cefoperazone, meropenem) in an aqueous environment was investigated by UV-visible absorption spectrophotometry. The results indicated that Fe3O4@ZIF-8@MBLs, compared to control ZIF-8, exhibited superior degradation ability, excellent reusability, and better stability under several harsh conditions. The strategy of combining ZIF-8 and MBLs to form magnetic porous polymers may be suitable for removing β-lactam antibiotics from an aqueous environment. This work provided an original insight into future studies on the degradation of β-lactam antibiotics employing MBLs immobilized by magnetic metal–organic frameworks.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2023
·Vol. 16
·期 7
·10026-10033
·
IF 9.9
Jiang Shihui,Ke Jianxi,Li Guowei,Liu Aijun,Li Xiaohan,Ye Lixiang,Jiang Feilong,Liu Yongsheng,Hong Maochun
Hexagonal-phase NaYF 4 (β-NaYF 4 ) has been acknowledged to be one of the most efficient doping hosts to prepare bright lanthanide-doped luminescent nano-bioprobes for various biomedical applications. However, to date, it remains a great challenge to synthesize ultra-bright lanthanide-doped β-NaYF 4 nano-bioprobes under a low reaction temperature by using conventional synthetic methods. Herein, we first develop an acetic acid (HAc)-mediated coprecipitation method for the preparation of ultra-bright lanthanide-doped β-NaYF 4 nanoprobes under a low reaction temperature at 200 °C. Based on a series of comparative spectroscopic investigations, we show that the use of HAc in the reaction environment can not only promote the rapid α–β phase transformation of NaYF 4 host at 200 °C within 1 h but also boost the absolute photoluminescence quantum yield (PLQY) of NaYF 4 nanocrystals to 30.68% for near-infrared emission and to 3.79% for upconversion luminescence, both of which are amongst the highest values for diverse lanthanide-doped luminescent nanocrystals ever reported. By virtue of their superior near-infrared luminescence, we achieve optical-guided dynamic vasculature imaging in vivo of the whole body at a high spatial resolution (23.8 µm) under 980 nm excitation, indicating its potential for the diagnosis and treatment evaluation of vasculature-related diseases.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）