CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
Weide Wang,Dongxu Yao,Hanqin Liang,Yongfeng Xia,Kaihui Zuo,Jinwei Yin,Yu-Ping Zeng
The effect of the GdH 2 on the shrinkage behavior, microstructure, thermal conductivity , and flexural strength was investigated. The pre-sintering enables the elimination of native SiO 2 and the simultaneous formation of Gd 2 O 3 . The N/O ratio in the liquid phase was increased because of the increasing Gd 2 O 3 /SiO 2 ratio, resulting in the restricted shrinkage during gas pressure sintering. The high N/O ratio in the liquid phase benefits not only the nucleation and growth of β-Si 3 N 4 but also the removal of lattice oxygen, leading to the increase of thermal conductivity . Although the replacement of Gd 2 O 3 by GdH 2 leads to enhanced flexural strength at low temperature, it tends to reduce the flexural strength at high temperature. This variation related largely to the exaggerated bimodal microstructure. This work signifies that GdH 2 is an effective sintering additive for obtaining Si 3 N 4 ceramics with both high flexural strength and high thermal conductivity.
研究领域:
材料科学
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陶瓷工程
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DOI
高影响因子
CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Yun Liu,Ruixiang Liu,Yuanhang Zheng,Jixue Zhao,Tianyi Sui,Xiaolei Li,Bin Lin
To investigate the mechanism of the influence of the sintering additive ratio on the microstructure evolution of silicon nitride (Si 3 N 4 ) ceramics, Si 3 N 4 ceramics were prepared by gas pressure sintering using different ratios of Y 2 O 3 /MgSiN 2 as additives. Varying the ratio of Y 2 O 3 /MgSiN 2 changed the composition of the liquid phase, thereby affecting the densification process and phase transformation rate of Si 3 N 4 ceramics, and ultimately changing their microstructure. The reduction in the Y 2 O 3 /MgSiN 2 ratio raised the N/O and Si/O ratios in the system, which increased the liquid phase viscosity. The Si 3 N 4 grain distribution varied from "normal to bimodal". In the case of increasing the proportion of MgSiN 2 and prolonging the sintering time, more of the glass phase evaporated, which reduced the content of the low thermal conductivity phase and increased the continuity of Si 3 N 4 /Si 3 N 4 . Therefore, Si 3 N 4 ceramics with 1 wt% Y 2 O 3 and 5 wt% MgSiN 2 exhibited excellent properties after sintering at 1900 °C for 12 h, with thermal conductivity, bending strength , and fracture toughness of 106.7 Wm −1 K −1 , 776 MPa, and 11.66 MPa m 1/2 , respectively.
研究领域:
材料科学
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陶瓷工程
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Kunlong Yang,Yue Luo,Tongzheng Sun,Han Qiu,Qingru Geng,Yongxin Li,Man Liu,Nancy P. Keller,Fengqin Song,Jun Tian
Nitric oxide (NO) is a signaling molecule with diverse roles in various organisms. However, its role in the opportunistic pathogen Aspergillus flavus remains unclear. This study investigates the potential of NO, mediated by metabolites from A. oryzae (AO), as an antifungal strategy against A . flavus . We demonstrated that AO metabolites effectively suppressed A . flavus asexual development, a critical stage in its lifecycle. Transcriptomic analysis revealed that AO metabolites induced NO synthesis genes, leading to increased intracellular NO levels. Reducing intracellular NO content rescued A . flavus spores from germination inhibition caused by AO metabolites. Furthermore, exogenous NO treatment and dysfunction of flavohemoglobin Fhb1, a key NO detoxification enzyme, significantly impaired A . flavus asexual development. RNA-sequencing and metabolomic analyses revealed significant metabolic disruptions within tricarboxylic acid (TCA) cycle upon AO treatment. NO treatment significantly reduced mitochondrial membrane potential (Δψm) and ATP generation. Additionally, aberrant metabolic flux within the TCA cycle was observed upon NO treatment. Further analysis revealed that NO induced S-nitrosylation of five key TCA cycle enzymes. Genetic analysis demonstrated that the S-nitrosylated Aconitase Acon and one subunit of succinate dehydrogenase Sdh2 played crucial roles in A . flavus development by regulating ATP production. This study highlights the potential of NO as a novel antifungal strategy to control A . flavus by compromising its mitochondrial function and energy metabolism.
研究领域:
微生物学
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分子生物学
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真菌学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Ranran Ma,Weiwei Peng,Yanli Xie,Nazish Muzaffar,Weibin Ma,Yuhui Yang,Qian Li,Hang Jia
Aflatoxin B1 (AFB1), the most toxic and prevalent mycotoxin in food and feed, and its hydroxylated metabolite aflatoxin M1 (AFM1), common in dairy products, pose serious health risks. Effective degradation of these toxins is thus a key goal in food safety. In this study, Bacillus subtilis HNGD-Mq02 was isolated and assessed for its ability to degrade AFB1 and AFM1. Under optimal conditions (50 °C, pH 7), the fermentation broth achieved 94.06 and 98.26% degradation, respectively. Ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC-MS) analysis confirmed that both mycotoxins were converted into less-toxic compounds. Zebrafish assays and Ames tests further verified the reduced toxicity of the degradation products. In practical application, the strain reduced AFB1 by 72.90% in corn flour and AFM1 by 42.25% in milk powder. Fermentation also led to partial improvements in nutritional quality. These results highlight the potential of B. subtilis HNGD-Mq02 for enhancing the safety and value of food and feed products.
研究领域:
食品科学
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微生物学
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毒理学
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生物技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2023
·
IF 5
Jingyi Zhang,Yu Wang,Yuxi Sun,Shuaishuai Sun,Zhenbang Xu,Jun Li,Ji Li,Xinglong Gong
The soft actuators capable of responding to multiple stimuli and adapting to changing environments have attracted growing interest in the flexible multifunctional materials. However, how to achieve high degree of freedom (DoF), precise control, and complex shape transformation of the multi-stimuli responsive soft actuator, still remains challenging. Here, we report a multi-responsive soft actuator with various controllable sophisticated deformations by integrating a magnetically sensitive elastomer (MSE) with a liquid crystal elastomer (LCE). Through regulating the stimuli strength and the geometrical dimensions and material parameters of elastomers, the bending angle and curling curvature of the actuator are accurately controlled ranging from 0 to 58.9° and from 0.23 to 1.29 cm–1, respectively. The facile material-structural synergistic design drives the complex 3D shape deformations (e.g., bidirectional bending, shrinkage/bending, rolling/bending, and twisting/bending) of the actuator. More importantly, due to its photosensitive characteristics, the shape-morphing of the actuator can be manipulated locally and sequentially, which markedly enriches the DoFs. The flower-shaped actuator displays multiple deformation modes, and the hand-shaped actuator transforms between 8 gestures under the control of laser and magnetic field, proving that the multi-responsive soft actuators have great application potentials in future bioengineering, soft manipulators, and flexible electronics.
研究领域:
材料科学
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机器人技术
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生物医学工程
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机械工程
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软物质物理
✓ 已验证
产品关联
DOI
高影响因子
Infection and Drug Resistance
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2025
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IF 2.9
Kaixuan Yuan,Benshun Tian,Annan Zhan,Zhuoxi Chen,Long Ye,Yong Ling,Suiling Liu,Xuejiao Bai,Yunhu Zhao
Purpose Inactivating filamentous fungi (molds) reduce toxin production and transmission. This study evaluated the efficacy of formic acid (FA) fumigation to identify optimal conditions and its effects on fungal morphology. We aim to develop an efficient inactivation protocol for molds and provide valuable insights into laboratory techniques for fungal inactivation.Methods Initially, 238 clinical isolates from 23 species of molds were evaluated for the inactivation efficacy of formic acid, formaldehyde, and peracetic acid. After identifying the optimal sterilizing agent, 188 isolates from 16 clinically significant species were tested. Four concentrations (30%, 50%, 70%, and 100%) and six-time intervals (4 to 14 hours) were tested to optimize fumigation conditions. Morphological changes were analyzed using Aspergillus fumigatus ATCC MYA-3626 as the standard strain.Results The inactivation rates of 238 molds fumigated with formic acid, formaldehyde, and peracetic acid for 24 hours were 99.16%, 89.08%, and 90.35% (P < 0.05), indicating that formic acid was more effective than formaldehyde and peracetic acid. Subsequently, 188 isolates from 16 species were fumigated with formic acid at concentrations of 30%, 50%, 70%, and 100% for 24 hours, resulting in inactivation rate of 73.9%, 98.4%, 100%, and 100% (P < 0.05). Inactivation rate increased significantly with longer exposure times: 51.1% at 4 hours, 80.9% at 6 hours, 89.4% at 8 hours, 94.7% at 10 hours, 100% at 12 hours, and 100% after 14 hours (P < 0.05). After fumigation, A. fumigatus ATCC MYA-3626 growth ceased. Electron microscopy revealed irregular folds and roughness on spore surfaces, as well as collapsed or dead spores.Conclusion Formic acid demonstrated superior inactivation efficacy compared to formaldehyde and peracetic acid. At a concentration of 70% and a fumigation duration of 12 hours, the inactivation of molds was most effective.
研究领域:
真菌学
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微生物学
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临床实验室技术
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真菌病理学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Lihui Zhang,Jialing Zhu,Shaojin Wang,Long Chen,Zihan Song,Lei Zhang,Haile Ma
Effectively controlling molds in dried shiitake mushrooms is important for food industry to maintain the product quality. To improve the edible safety of shiitake mushrooms, this study applied radio frequency (RF) energy combined with natamycin to inactivate Aspergillus niger spores, and evaluate the quality changes after the treatment. The results showed that the population of A. niger spores in shiitake mushrooms with different moisture contents (MCs) could achieve a 5-log (CFU/g) reduction after the combined treatment at different temperatures. Weibull distribution ( R 2 = 0.978 to 1.000) provided a better fit for the inactivation kinetics of A. niger spores than Linear model ( R 2 = 0.954 to 0.990) and Logistic one ( R 2 = 0.953 to 0.988). Meanwhile, the three-dimensional inactivation curves were constructed based on the sample MCs, RF heating time, and holding time. In addition, the MCs of treated shiitake mushrooms decreased by 4.12%–7.66% (w.b.) after natural cooling, achieving synchronous drying. There was no significant difference in textural properties of shiitake mushrooms after the combined treatment. The changes in color, structure, rehydration ratio, and total sugar content were affected by the treatment time and temperatures. As one of the main aroma substances, the content of sulfur-containing compounds in shiitake mushrooms after treatment increased compared to that of the control samples. This study indicates that the combination of RF energy and natamycin is feasible for inactivating pathogens in shiitake mushroom and ensuring the safety of their consumption.
研究领域:
食品科学
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微生物学
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产品关联
DOI
高影响因子
Toxins
·
2024
·Vol. 17
·期 1
·11
·
IF 3.9
Hui-Qin Pan,Rui Feng,Yan-Nan Tan,Xiao-Ya Qin,Yi-Min Cao,Xiu-Hong Mao,Qing Hu,Heng Zhou
Puberulic acid (PA) is a mycotoxin produced by a species ofPenicillium. It has received widespread attention as a significant contributor to the reported fatalities associated with red yeast rice dietary supplements. However, the detection of PA, especially at low concentration levels, poses a considerable challenge, with no detection methods reported thus far. Here, we present a simple and sensitive derivatization-based LC-MS/MS method, requiring no purification processes, for determination of PA in the red yeast rice. The methylating derivatization with trimethylsilyldiazomethane (TMSCHN2) was performed to enhance its analytical performance. To achieve optimal detection sensitivity, the amount of solvent and TMSCHN2for the derivatization reaction, along with the reaction time, were individually optimized. Moreover, sample extraction solvent was carefully chosen to improve recoveries in real sample analyses. Comparatively, the proposed LC-MS/MS method achieved a superior detection sensitivity, over 100-fold higher than that of the liquid chromatography method. A good linear relationship within the concentration range of 5 ng/mL to 200 ng/mL (with a linear correlation coefficient of 0.99952) was demonstrated by the method validation. The average recovery rate was between 82.2% and 84.2%, and the repeatability (RSD of 2.1% to 10.4%, n = 6) was satisfactory. The derivatized PA remained stable within 48 h. The limit of detection and the limit of quantification could reach 2 μg/kg and 50 μg/kg, respectively. As a result, the method was successfully applied to detect PA in over 42 batches of the red yeast rice samples. It indicated a low risk of PA contaminations in the red yeast rice products made in China. Furthermore, its application to the other health food products containing red yeast rice demonstrated the applicability of the established method.
研究领域:
食品科学
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分析化学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Biomolecules
·
2021
·Vol. 11
·期 8
·1133
·
IF 4.879
Kevin Adi Kurnia,Fiorency Santoso,Bonifasius Putera Sampurna,Gilbert Audira,Jong-Chin Huang,Kelvin H.-C. Chen,Chung-Der Hsiao
Tail coiling is a reflection response in fish embryos that can be used as a model for neurotoxic analysis. The previous method to analyze fish tail coiling is largely based on third-party software. In this study, we aim to develop a simple and cost-effective method called TCMacro by using ImageJ macro to reduce the operational complexity. The basic principle of the current method is based on the dynamic change of pixel intensity in the region of interest (ROI). When the fish tail is moving, the average intensity is increasing. In time when the fish freeze, the peak of mean intensity is maintaining at a relatively low level. By using the optimized macro settings and excel VBA scripts, all the tail coiling measurement processes can be archived with few operation steps with high precision. Three major endpoints of tail coiling counts, tail coiling duration and tail coiling intervals can be obtained in batch. To validate this established method, we tested the potential neurotoxic activity of Tricaine (methanesulfonate, MS-222) and psychoactive compound of caffeine. Zebrafish embryos after Tricaine exposure displayed significantly less tail coiling activity in a dose-dependent manner, and were comparable to manual counting through the Wilcoxon test and Pearson correlation double validation. Zebrafish embryos after caffeine exposure displayed significantly high tail coiling activity. In conclusion, the TCMacro method presented in this study provides a simple and robust method that is able to measure the relative tail coiling activities in zebrafish embryos in a high-throughput manner.Keywords:ImageJ;chorion;tail coiling;ROI;zebrafish;embryo
研究领域:
发育生物学
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毒理学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CONTROL
·
2015
·
IF 2.806
Yanzhen Wang,Xiaobin Zeng,Zhengkun Zhou,Ke Xing,Akalate Tessema,Hong Zeng,Jun Tian
The antifungal activity of Nerol (NEL) was evaluated against Aspergillus niger , a known cause of grape spoilage, and possible modes of action were explored. The antifungal efficacy of NEL against A. niger has been proven in a dose-dependent manner through in vitro (mycelial growth and spore germination) and in vivo (grapes) tests. An obvious increase in the membrane permeability was detected through the determination of extracellular pH and conductivity. The damage in the cell membrane was measured by monitoring the influx of Propidium iodide (PI) using flow cytometry and was further verified through the inhibition of ergosterol synthesis in the cell membrane. NEL induced morphological changes, and the integrity of the A. niger fungal cells decreased with increasing NEL concentrations. A dose-dependent decrease in ergosterol production was also seen in A. niger cell membranes upon exposure to NEL. The mechanism-of-action study revealed that NEL activated a membrane-active mechanism that inhibits ergosterol synthesis and consequently compromised the membrane integrity of A. niger . As a result, the membrane permeability changes and causes cell death. The membrane lesion mechanism makes NEL a natural alternative to commercial fungicide for control of stored grape spoilage.
研究领域:
食品科学
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微生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2024
·
IF 5.8
Zhouxi Tan,Mingkang Zhang,Shuai Zhao,Yihua Huang,Zhengren Huang
Low strength and poor performance of SiC–WC composite for seals, attributable to the use of a large amount of liquid phase sintering additives, limit the application prospects of these materials. This study focuses on preparing high-strength, high-toughness, and wear-resistant SiC–WC composite using a small amount of aluminum and carbon black as sintering additives. The results revealed that the specimen containing 50 wt% WC exhibited the maximum flexural strength and fracture toughness of 645.9 MPa and 7.49 Mpa m 1/2 , respectively. These improvements were attributed to the deoxidizing and solid-solution effects of Al and the reinforcing effect of the WC second phase. Moreover, the specific wear rate was measured at 8.06 × 10 −7 mm 3 N −1 m −1 , which was 56.9 % lower than that of monolithic SiC. Results from Raman spectral analysis provided evidence of the presence of W–O bonds on the wear surface, indicating that the SiO 2 film could be successfully replaced by a WO 3 film.
研究领域:
材料科学
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机械工程
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产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 5
·835
·
IF 5.1
Chuling Liu,Li Cheng,Mingtian Yang,Zhengli He,Yanan Jia,Li Xu,Yuansong Zhang
Monascusis a fungus widely used in food fermentation. This study employed microbial technology, combined with microscopic morphological observations and ITS sequence analysis, to isolate, purify, and identify 10 strains of red yeast mold from variousMonascusproducts. After the HPLC detection of metabolic products, the M8 strain containing the toxic substance citrinin was excluded. Using the EWM-TOPSIS model, the remaining nine safeMonascusstrains were evaluated for their inhibitory activities against pancreatic lipase, α-glucosidase, α-amylase, and the angiotensin-converting enzyme. The M2 strain with the highest comprehensive scores for lowering blood sugar, blood lipids, and blood pressure was selected. Its fermentation product at a concentration of 3 mg/mL had inhibition rates of 96.938%, 81.903%, and 72.215%, respectively. The contents of the blood lipid-lowering active substance Monacolin K and the blood sugar and blood pressure-lowering active substance GABA were 18.078 mg/g and 5.137 mg/g, respectively. This strain can be utilized for the biosynthesis of important active substances such as Monacolin K and GABA, as well as for the fermentation production of safe and effective functional foods to address health issues like high blood lipids, high blood sugar, and high blood pressure in people. This study also provides insights into the use of natural fungi to produce healthy foods for combating chronic diseases in humans.
研究领域:
微生物学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）