INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Jiaojiao Su,Yaning Zhang,Mingli Yue,Jian Liu
Cell chirality is a complex phenomenon that imparts unique properties. The creation of biomimetic artificial cells chirality is a rapidly evolving research topic that has had a profound impact in multiple fields, including materials science, catalysis, biotechnology, and biomedicine. Herein, we report a simple method that combines L- or D-penicillamine (L/D-Pen) with proteinosomes on the surface of gold nanoparticles to afford nanoparticle hybrid microcapsules. Encapsulation of the enzyme in the microcapsule enables a chiral recognition effect on the membrane surface, thereby affording control over the enantioselective catalytic reaction of the d -glucose (D-Glu) substrate loaded inside the hybrid microcapsule. The adsorption energy of D-Glu and D-Pen (−0.095) calculated by MS is less than that of D-Glu and L-Pen (−0.064). Thus enantiomeric selective recognition is a stronger interaction force than adsorption for the same configuration. These findings highlight the development of biomimetic chiral microcompartments, and provide a platform for driving cellular chirality in various organisms.
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VIBRATIONAL SPECTROSCOPY
·
2022
·
IF 2.382
Chang Wang,Qingqiang Cui,Tian Yue,Anxin Jiao,Hui Ma,Mengya Zhang,Linqi Zheng,Shuang Li,Guanhua Li,Ming Chen
As for the ultrasensitive diagnosis of biomolecules via surface-enhanced Raman scattering spectroscopy (SERS), the primary requirement is to effectively enhance the activity of nano-substrates. Herein, we report an interesting strategy for further boosting SERS activity by thermal annealing treatment of as-prepared hybrid substrates. Initially, highly dense and monodisperse plasmonic Au nanoparticles (NPs) were loaded on two-dimensional (2D) graphitic carbon nitride (g-C 3 N 4 ) nanosheets . By adopting uric acids (UAs) as probe biomolecules, the g-C 3 N 4 /Au NPs with appropriate ~2.1% Au compositions exhibit much higher Raman signals than that of the hybrids with other Au contents. Besides the enhancement by routinely controlling hybrid components, the further maximizing SERS activity with a ~2.6-fold improvement than the original one can be obtained by thermal annealing of g-C 3 N 4 /Au NPs at 350 ℃ condition. It enables the thermal modified g-C 3 N 4 /Au NPs to be applied for highly sensitive monitoring of UAs in artificial urine. The corresponding detection limit is achieved at the ultralow level of 10 −11 M, which is nearly two orders of magnitude better than that of the unannealed sample. It should be attributed to the stronger synergistic coupling effect by thermal annealing treatment that gives rise to the higher-efficiency photoinduced electron transfer (PIET) between g-C 3 N 4 supports and plasmonic Au NPs. The present work provides a new opportunity for further promoting the SERS activity of prefabricating nano-substrates, holding great potential for practical monitoring of ultra-trace biomolecules in the near future.
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ANALYTICAL CHEMISTRY
·
2023
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IF 7.4
Fanglei Liu,Jiayi Zhao,Xinyu Liu,Xi Zhen,Qiumei Feng,Yingqiu Gu,Guohai Yang,Lulu Qu,Jun-Jie Zhu
In this paper, a photoelectrochemical (PEC)-surface-enhanced Raman scattering (SERS) dual-mode biosensor is constructed coupled with a dual-recognition binding-induced DNA walker with a carbon nitride nanosheet (C3N4)/MXene-gold nanoparticles (C/M-Au NPs) accelerator, which is reliable and capable for sensitive and accurate detection of Staphylococcus aureus (S. aureus). Initially, a photoactive heterostructure is formed by combining C3N4 and MXene via a simple electrostatic self-assembly as they possess well-matched band-edge energy levels. Subsequently, in situ growth of gold nanoparticles on the formed surface results in better PEC performance and SERS activity, because of the synergistic effects of surface plasmon resonance and Schottky barrier. Furthermore, a three-dimensional, bipedal, and dual-recognition binding-induced DNA walker is introduced with the formation of Pb2+-dependent DNAzyme. In the presence of S. aureus, a significant quantity of intermediate DNA (I-DNA) is generated, which can open the hairpin structure of Methylene Blue-tagged hairpin DNA (H-MB) on the electrode surface, thereby enabling the switch of signals for the quantitative determination of S. aureus. The constructed PEC-SERS dual-mode biosensor that can be mutually verified under one reaction effectively addresses the problem of the low detection accuracy of traditional sensors. Experimental results revealed that the effective combination of PEC and SERS is achieved for amplification detection of S. aureus with a detection range of 5–108 CFU/mL (PEC) and 10–108 CFU/mL (SERS), and a detection of limit of 0.70 CFU/mL (PEC) and 1.35 CFU/mL (SERS), respectively. Therefore, this study offers a novel and effective dual-mode sensing strategy, which has important implications for bioanalysis and health monitoring.
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RSC Advances
·
2021
·Vol. 11
·期 32
·19813-19818
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IF 3.361
Yazhou Qin,Yuanzhao Wu,Binjie Wang,Jiye Wang,Xingsen Zong,Weixuan Yao
Branched Au nanoparticles (Au NPs) can significantly enhance the Raman signal of trace chemical substances, and have attracted the interest of researchers. However, there are still challenges to accurately prepare the morphology of branched Au NPs. In this work, we have successfully prepared sea urchin-like Au NPs and Au nanowires by using the seed-mediate growth method, with cetyltrimethylammonium bromide (CTAB) and glutathione as ligands, and ascorbic acid as a reducing agent. Using Au NPs with a tetrahexahedron (THH) morphology as seeds, and by simply changing the concentration of glutathione, we explored the growth process of sea urchin-like Au and Au nanowires. At low concentrations of glutathione, Au NPs will preferentially grow along the edges and corners of the THH Au seed, forming a core/satellite structure. As the concentration of glutathione increases, Au NPs will grow along the direction of glutathione, forming sea urchin-like Au NPs. To further increase the concentration of glutathione, we will prepare Au nanowires. In addition, we use the prepared Au NPs as a substrate material for surface-enhanced Raman (SERS) high-sensitivity detection. By using 4-aminothiophenol (4-ATP) as the test molecule, we evaluated the SERS effect of the prepared Au NPs with different morphologies. The results showed that sea urchin-like Au NPs have the best enhancement effect. The lowest concentrations of Rhodamine 6G and 4-ATP were 10−10 M and 10−12 M, respectively, using sea urchin Au NPs as the base material. Furthermore, we conducted a highly sensitive SERS detection of the poison atropine monohydrate, and the lowest detected concentration was 10−10 M.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
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IF 4.539
Qixia Guan,Hao Guo,Ning Wu,Yujuan Cao,Mingyue Wang,Longwen Zhang,Wu Yang
As a new kind of porous organic material, covalent organic framework materials (COFs) have many advantages and have been widely used in catalysis, sensing, drug delivery, gas separation and other fields. In this paper, A COF was successfully prepared based on Schiff base reaction of 1, 3, 6, 8-tetrakis(4-formyl phenyl) pyrene (TFPPy) and 1, 3, 6, 8-tetrakis(4-amino phenyl) pyrene (PyTTA) named as TF-Py COF, and its structure was characterized in detail. By physically combining a pre-prepared three-dimensional carbon material containing nitrogen (3D NCNF-T) and TF-Py COF, followed by deposition of AuNPs, relatively poor conductivity of TF-Py COF was efficiently overcome and a novel electrochemical sensing platform with high sensitivity and high selectivity for simultaneous detection of acetaminophen (ACOP) and 4-aminophenol (4-AP) was successfully constructed. The synergistic effect between TF-Py COF, 3D NCNF-T and AuNPs effectively improves the analytical sensitivity of target analytes. Under optimized experimental conditions, the electrochemical sensing platform has a sensitive volt-ampere response and a wide linear range to both ACOP and 4-AP, and the detection limits are 0.13 μM and 0.05 μM (S/N = 3), respectively. The sensor has been successfully applied to the determination of ACOP and 4-AP in acetaminophen tablets and Yellow River water samples, with high recovery rate, good selectivity and reproducibility, and strong anti-interference ability. It is expected to be widely used in the actual sample detection.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yuchen Hou,Yongjiao Sun,Yimeng Dong,Bingliang Wang,Zhiyuan Lu,Zihan Wei,Zhaomin Tong,Wendong Zhang,Koichi Suematsu,Jie Hu
A series of Au NPs decorated hierarchical In 2 O 3 /SnIn 4 S 8 microcubes were prepared by in-situ growing SnIn 4 S 8 nanosheets on the surface of In 2 O 3 microcubes and followed by reducing Au NPs on the surface of the hierarchical composite. The gas sensing performance of the sensors based on the as-prepared materials were systematically assessed. And the results showed that coverage of SnIn 4 S 8 and introduction of appropriate amount of Au NPs improved the gas sensing response of In 2 O 3 toward NO 2 at room temperature (RT). In particular, the sensor fabricated with 3 wt% Au NPs decorated In 2 O 3 /SnIn 4 S 8 exhibited excellent selectivity (S NO2 /S other > 12.6), high response (155.65–7 ppm NO 2 ), low theoretical limit of detection (LOD, 21.43 ppb), fast response/recovery time (132/191 s), good anti-interference performance, as well as repeatability and stability. The outstanding sensing performance at RT might be associated with a synergistic effect of heterostructures and the spillover effect of Au NPs, which could enlarge the electron depletion layer and promote the adsorption and dissociation of oxygen and nitrogen dioxide molecules.
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MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Guoliang Zhang,Cong Xue,Ruxangul Jamal,Tursun Abdiryim,Feng Xu,Qian Cheng
L-Tyrosine (L-Tyr) is one of the 21 essential amino acids for the human body. Its accurate detection is of great clinical and practical significance in monitoring, food quality and safety. Functional groups critically govern electrochemical sensor performance by mediating target adsorption. Conventional poly (3,4-ethylenedioxythiophene) (PEDOT)-based sensors face limited flexibility and active site accessibility. Amino-grafted PEDOT was developed by in situ growth on Ti 3 C 2 T X nanosheets, incorporating small-sized Au nanoparticles to create a highly conductive and active substrate for selective detection of L-Tyr. The resulting molecularly imprinted electrochemical sensor, fabricated on the PEDOT-CH 2 NH 2 /Ti 3 C 2 T X /Au NPs/GCE platform, exhibited enhanced catalytic activity and efficient L-Tyr enrichment. The combination of components improved electron transfer, MIP stability, and imprinting cavity accessibility. The sensor demonstrated a broad linear range (10 −6 –100 μM) with an ultralow detection limit (3.64 × 10 −6 μM), and exhibited excellent selectivity, reproducibility, and operational stability. Practical tests in milk matrices confirmed high recovery rates (96.65 %–101.35 %), showcasing its potential for use in complex food systems and it also has detection capabilities similar to those of standard methods. This work provides a sensitive and reliable strategy for amino acid monitoring in food, with significant applications in food safety assurance.
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JOURNAL OF HAZARDOUS MATERIALS
·
2021
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IF 10.588
Hanyu Wang,Yanting Qi,Dan Wu,Qin Wei
In this study, lead(II) sulfide (PbS) nanocrystals were modified on nickel(II)oxide nanosheets (NiO NSs) via the chemical bath method. Afterwards, Au nanoparticles (NPs) were also modified successfully. A photoelectrochemical (PEC) self-powered platform for detecting sarcosine with high PEC activity was constructed. The capacity of NiO NSs to be loaded with other sensitizing materials was mainly attributed to its porous structure and large specific surface area. Under optimum conditions, the constructed PEC self-powered cathodic sensor for detecting sarcosine exhibited a linear range in 5.0 × 10 −8 –5.0 × 10 −2 mol/L with a detection limit (LOD) of 1.7 × 10 −8 mol/L. The biosensor demonstrated good reproducibility, acceptable stability and high specificity, thus confirming its potential application in the detection of other similar substances.
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Biosurface and Biotribology
·
2025
·Vol. 11
·期 1
·e12090
·
IF 1.8
Yi Duan,Qi Wu,Jiangtao Lin,Yourong Duan,Qi Wang,Yuanyuan Li
In this study, the authors designed a paper-based electrochemical immunodevice modified with copper embedded in copper sulphide hollow nanocages wrapped with Au nanoparticles (Cu@CuS@Au NPs) for the specific detection of prostate-specific antigen (PSA), aiming to advance point-of-care testing. The large specific surface area of Cu@CuS nanocages enables efficient capture of biotin antibodies, leading to the direct amplification of the signal through the inhibition of electron transport in the redox process of Cu, eliminating the need for universal redox electron mediators. Additionally, Au NPs on the surface of Cu@CuS can accelerate charge transfer and conjugate with anti-PSA. The hierarchical morphology and structure of Cu@CuS nanocages were characterised using scanning electron microscopy and transmission electron microscopy. The fabrication process of the immunodevice was monitored using cyclic voltammetry and electrochemical impedance spectroscopy analyses. PSA was sensitively detected using differential pulse voltammetry on this proposed immunodevice within a linear range from 0 to 100 ng/ml ( R 2 = 0.996), achieving a low detection limit of 0.077 ng/ml. In addition, the practicality of the developed immunosensor has been proven by successfully detecting PSA in human serum samples obtained from clinical settings. The integration of electrochemical sensors and microfluidic devices holds promise for developing cost-effective approaches in clinical immunoassays.
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Analytical Methods
·
2023
·Vol. 15
·期 37
·4842-4850
·
IF 3.1
Zhiyou Zeng,Xiaoyu Yang,Yongguo Cao,Sanshan Pu,Xinyu Zhou,Rongli Gu,Yiqian Zhang,Caijun Wu,Xiaojun Luo,Yi He
Nowadays, the presence of highly toxic and persistent residues of pesticides in water and food around the world is becoming a serious problem, and so their rapid and sensitive detection is critical to human health. In this work, a 3D composite nanoparticle of porous PDA (polydopamine) microspheres and Au NPs (PPDA@Au NPs) was proposed as a SERS substrate to detect pesticides. Porous PDA as a substrate was first synthesized with F127 (Pluronic F127), dopamine hydrochloride, and 1,3,5-TMB (1,3,5-trimethylbenzene) under weakly alkaline conditions by a one-step method. Then, HAuCl4 was in situ reduced in the pores of PPDA spheres and grew sequentially for effecting the reducibility of PPDA. As a result, uniform 3D PPDA@Au NPs with “hot spots” were successfully synthesized as SERS substrates, which could effectively avoid the agglomeration of gold nanoparticles to greatly improve the sensitivity and uniformity of the SERS platform. At the same time, methyl parathion, 4-chlorophenol, and 2,4-D as representatives of pesticides were detected with the proposed PPDA@Au NP-based SERS platform, with detection limits lower than 7.26, 7.52, and 11 ng mL−1, separately. The current work presents a simple preparation method to prepare sensitive and uniform SERS platform PPDA@Au NPs, which have potential for applications in actual pesticide and drug testing.
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TALANTA
·
2022
·
IF 6.556
Xiaoli Wang,Junwang Ji,Pengfei Yang,Xinyu Li,Yiquan Pang,Panpan Lu
The detection of cancer markers still has shortages of low sensitivity, time-consuming operation, the use of unstable and expensive antibodies. In this work, a novel Love-mode surface acoustic wave (LSAW) aptasensor with dummy fingers based on the monolayer molybdenum disulfide/gold nanoparticles (monolayer MoS 2 /Au NPs) was developed for the highly sensitive and rapid determination of alpha-fetoprotein (AFP) in serum. Interdigital electrodes (IDTs) with dummy fingers were designed and applied to improve the acoustic characteristic of the LSAW aptasensor. The less energy dissipation and wave-front distortion of the LSAW aptasensor were confirmed by COMSOL simulation and test results. The newly-developed sensing film monolayer MoS 2 /Au NPs/Apt/6-mercaptohexanol (MCH) was applied for the specific detection of AFP and significantly improved the sensitivity of the LSAW aptasensor. The excellent performance of the LSAW aptasensor allowed the sensitive and rapid detection of AFP in serum in the range of 0.01 ⁓ 100 ng/mL with a low detection limit of 4.79 pg/mL. Additionally, the proposed LSAW aptasensor exhibited excellent selectivity , long-term stability, and reproducibility, and could be used to detect other cancer biomarkers.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Hangqing Xie,Haogang Zhu,Lei Lei,Xiaolei Yang,Youjie Hua,Renguang Ye,Gongxun Bai,Shiqing Xu
Surface-enhanced Raman scattering (SERS) has attracted much attention with its powerful trace detection and analysis capabilities, especially biological and environmental molecules. However, building a protein SERS detection platform based on semiconductor devices is a huge challenge. Herein, through the synergy of NH 3 and nickel foam, a large-sized semiconductor tungsten oxide hydrate platform (WOHP) was synthesized. The crystal plane of a single WOHP particle is larger than the excitation spot. As a SERS substrate, WOHP can make full use of the excitation light without destroying the structure during the protein molecules detection process. Through the synergy of WOHP and Au NPs, the enhancement factor is 1.5 × 10 4 . Raman peaks of WOHP can be used as references for the detection of typical protein cytochrome C (Cyt C). As the Cyt C concentration decreases, the I Cyt C /I WOHP ratio decreases, and the signal can still be obtained when the concentration is as low as 5 × 10 −9 mol L −1 . More importantly, the method does not affect the catalytic activity of Cyt C and can be applied to the detection of Cyt C concentration in serum.
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